Hypocrea atroviridis (strain ATCC 20476 / IMI 206040) (Trichoderma atroviride)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma; Trichoderma atroviride

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11815 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9NZ68|G9NZ68_HYPAI MFS domain-containing protein OS=Hypocrea atroviridis (strain ATCC 20476 / IMI 206040) OX=452589 GN=TRIATDRAFT_79036 PE=3 SV=1
MM1 pKa = 7.47AVLSKK6 pKa = 10.39LLPVAAVMFGCVNPATALAVPEE28 pKa = 4.43RR29 pKa = 11.84GQRR32 pKa = 11.84WDD34 pKa = 3.17VHH36 pKa = 5.04YY37 pKa = 10.55HH38 pKa = 4.75VSLNSRR44 pKa = 11.84LNSKK48 pKa = 9.33GFPPMPHH55 pKa = 5.76EE56 pKa = 4.68AGRR59 pKa = 11.84NASAVLAEE67 pKa = 4.56RR68 pKa = 11.84GEE70 pKa = 4.19EE71 pKa = 3.88HH72 pKa = 7.66DD73 pKa = 5.35ADD75 pKa = 4.87CDD77 pKa = 4.27CQVHH81 pKa = 5.39TVILTQKK88 pKa = 10.44DD89 pKa = 3.37YY90 pKa = 11.72DD91 pKa = 4.36RR92 pKa = 11.84IQSEE96 pKa = 5.13GEE98 pKa = 4.08GHH100 pKa = 7.23PSPSTHH106 pKa = 6.14GKK108 pKa = 7.52TSEE111 pKa = 4.05NGNHH115 pKa = 5.92NEE117 pKa = 3.98RR118 pKa = 11.84GGSGEE123 pKa = 4.03YY124 pKa = 10.16KK125 pKa = 10.49EE126 pKa = 4.23SAKK129 pKa = 10.78YY130 pKa = 10.36YY131 pKa = 10.4SEE133 pKa = 4.44NGTSGEE139 pKa = 4.0YY140 pKa = 10.08RR141 pKa = 11.84EE142 pKa = 4.21SAKK145 pKa = 10.96YY146 pKa = 10.05SDD148 pKa = 3.8NGASVEE154 pKa = 4.0HH155 pKa = 6.67RR156 pKa = 11.84ASGKK160 pKa = 10.08YY161 pKa = 9.81GGSGNHH167 pKa = 5.76YY168 pKa = 10.87EE169 pKa = 3.97NGTYY173 pKa = 10.3GGSGNHH179 pKa = 5.75YY180 pKa = 10.87EE181 pKa = 3.97NGTYY185 pKa = 10.26GGSGRR190 pKa = 11.84YY191 pKa = 8.53EE192 pKa = 3.85EE193 pKa = 4.2NDD195 pKa = 3.1NYY197 pKa = 11.18GEE199 pKa = 4.07SGRR202 pKa = 11.84YY203 pKa = 8.38EE204 pKa = 4.13EE205 pKa = 4.37NDD207 pKa = 3.1NYY209 pKa = 11.18GEE211 pKa = 4.02SGRR214 pKa = 11.84YY215 pKa = 8.43EE216 pKa = 4.19EE217 pKa = 4.99IGQHH221 pKa = 6.27GEE223 pKa = 3.27IDD225 pKa = 3.81NYY227 pKa = 9.53GGRR230 pKa = 11.84YY231 pKa = 9.1KK232 pKa = 10.7EE233 pKa = 3.95VGKK236 pKa = 11.06YY237 pKa = 8.86GANGNHH243 pKa = 6.66GEE245 pKa = 4.05NEE247 pKa = 4.22KK248 pKa = 11.13YY249 pKa = 10.74GEE251 pKa = 4.1NGNYY255 pKa = 9.71GDD257 pKa = 3.51NGYY260 pKa = 10.29YY261 pKa = 10.18GKK263 pKa = 9.99NVDD266 pKa = 4.81GIADD270 pKa = 3.85QGPPGPPGPQGPPGPPGPQGNRR292 pKa = 11.84GPQGYY297 pKa = 7.63TGTKK301 pKa = 9.72GDD303 pKa = 4.05QGTQGPQGLTGSQGPAGSRR322 pKa = 11.84GPQGFTGPKK331 pKa = 9.26GDD333 pKa = 3.88QGIQGFPGATGSQGPQGNRR352 pKa = 11.84GPQGFTGPKK361 pKa = 9.63GDD363 pKa = 4.45QGNQGPQGLTGSQGPAGNRR382 pKa = 11.84GPQGLTGPAGPTGADD397 pKa = 3.34GAVGPVGPVGPVGPVGPAGADD418 pKa = 3.45GADD421 pKa = 3.82GAVGPAGPAGADD433 pKa = 3.55GAVGPVGPVGPVGPAGADD451 pKa = 3.45GADD454 pKa = 3.82GAVGPAGPAGADD466 pKa = 3.55GAVGPVGPAGADD478 pKa = 3.49GAVGPTGDD486 pKa = 4.83AGPEE490 pKa = 4.33GPIGPAGPAGADD502 pKa = 3.66GAVGPAGPVGPTGDD516 pKa = 4.35AGPEE520 pKa = 4.26GPAGADD526 pKa = 3.45GAVGPVGPAGPAGDD540 pKa = 3.95VGPTGDD546 pKa = 4.82AGPEE550 pKa = 4.43GPAGPAGPAGADD562 pKa = 3.54GAVGPTGADD571 pKa = 3.36GADD574 pKa = 3.9GADD577 pKa = 4.07GAPGPAGPAGPAGPAGADD595 pKa = 3.38GAVGPEE601 pKa = 4.47GPIGPAGADD610 pKa = 3.56GTDD613 pKa = 3.65GADD616 pKa = 3.59GAVGPAGPAGPIGPAGPAGADD637 pKa = 3.66GAVGPAGPAGADD649 pKa = 3.55GAVGPVGPAGPVGPTGDD666 pKa = 4.35AGPEE670 pKa = 4.26GPAGADD676 pKa = 3.54GAVGPAGPAGPAGDD690 pKa = 4.18VGPTGDD696 pKa = 4.89PGPEE700 pKa = 4.26GPAGPAGADD709 pKa = 3.66GAVGPAGPVGPAGDD723 pKa = 3.77VGPAGDD729 pKa = 5.0AGPEE733 pKa = 4.45GPAGPAGADD742 pKa = 3.66GAVGPAGPAGADD754 pKa = 3.51GADD757 pKa = 3.76GADD760 pKa = 3.77GAVGPAGPAGPAGPAGPAGADD781 pKa = 3.63GTDD784 pKa = 3.63GTDD787 pKa = 3.49GAVGPAGPAGPAGADD802 pKa = 3.66GAVGPAGPAGPAGADD817 pKa = 3.58GTDD820 pKa = 3.65GADD823 pKa = 3.59GAVGPAGPAGPAGADD838 pKa = 3.66GAVGPAGPAGADD850 pKa = 3.66GAVGPAGPAGPTGPTGPQGPQGPQGDD876 pKa = 3.94IADD879 pKa = 4.19YY880 pKa = 10.91DD881 pKa = 4.03YY882 pKa = 11.44LSCFTSPGDD891 pKa = 3.79STNGLAAPFATFVEE905 pKa = 5.05TVSIDD910 pKa = 3.64DD911 pKa = 4.96CATQCATIRR920 pKa = 11.84PGNPALYY927 pKa = 10.14FSLATVAGDD936 pKa = 4.04SVCSCGDD943 pKa = 3.4ALAADD948 pKa = 4.07ATANDD953 pKa = 3.73GHH955 pKa = 6.61NICNTQCTFTGQSGRR970 pKa = 11.84PVFCGGAGVVSVFASVV986 pKa = 2.7

Molecular weight: 90.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9NJ98|G9NJ98_HYPAI Protein kish OS=Hypocrea atroviridis (strain ATCC 20476 / IMI 206040) OX=452589 GN=TRIATDRAFT_255048 PE=3 SV=1
MM1 pKa = 7.87PSHH4 pKa = 5.85KK5 pKa = 9.4TFRR8 pKa = 11.84TKK10 pKa = 10.46QKK12 pKa = 9.86LAKK15 pKa = 9.55AQKK18 pKa = 8.93QNRR21 pKa = 11.84PVPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11770

45

11815

5388752

43

21901

456.1

50.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.836 ± 0.019

1.277 ± 0.009

5.763 ± 0.016

6.083 ± 0.02

3.815 ± 0.015

6.804 ± 0.025

2.365 ± 0.009

5.171 ± 0.015

4.932 ± 0.022

9.028 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.009

3.76 ± 0.011

5.724 ± 0.024

4.055 ± 0.018

5.876 ± 0.018

8.199 ± 0.024

5.729 ± 0.015

6.038 ± 0.017

1.509 ± 0.008

2.817 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski