Amino acid dipepetide frequency for Vibrio phage VspDsh_1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.59AlaAla: 8.59 ± 1.531
0.577AlaCys: 0.577 ± 0.204
4.836AlaAsp: 4.836 ± 0.696
5.558AlaGlu: 5.558 ± 1.076
2.743AlaPhe: 2.743 ± 0.391
5.341AlaGly: 5.341 ± 0.837
1.371AlaHis: 1.371 ± 0.316
4.114AlaIle: 4.114 ± 0.537
6.424AlaLys: 6.424 ± 1.179
7.579AlaLeu: 7.579 ± 0.931
2.382AlaMet: 2.382 ± 0.501
3.97AlaAsn: 3.97 ± 0.659
3.393AlaPro: 3.393 ± 0.653
3.176AlaGln: 3.176 ± 0.537
3.898AlaArg: 3.898 ± 0.651
4.331AlaSer: 4.331 ± 0.524
6.641AlaThr: 6.641 ± 0.587
5.775AlaVal: 5.775 ± 0.594
1.444AlaTrp: 1.444 ± 0.249
2.815AlaTyr: 2.815 ± 0.59
0.0AlaXaa: 0.0 ± 0.0
Cys
0.722CysAla: 0.722 ± 0.271
0.072CysCys: 0.072 ± 0.061
0.866CysAsp: 0.866 ± 0.268
1.155CysGlu: 1.155 ± 0.3
0.361CysPhe: 0.361 ± 0.166
0.794CysGly: 0.794 ± 0.249
0.722CysHis: 0.722 ± 0.296
0.794CysIle: 0.794 ± 0.261
0.722CysLys: 0.722 ± 0.229
1.011CysLeu: 1.011 ± 0.242
0.65CysMet: 0.65 ± 0.195
0.722CysAsn: 0.722 ± 0.228
0.577CysPro: 0.577 ± 0.182
0.361CysGln: 0.361 ± 0.139
1.083CysArg: 1.083 ± 0.325
1.227CysSer: 1.227 ± 0.336
0.577CysThr: 0.577 ± 0.22
0.361CysVal: 0.361 ± 0.153
0.0CysTrp: 0.0 ± 0.0
0.289CysTyr: 0.289 ± 0.13
0.0CysXaa: 0.0 ± 0.0
Asp
4.259AspAla: 4.259 ± 0.466
0.722AspCys: 0.722 ± 0.21
4.042AspAsp: 4.042 ± 0.807
3.898AspGlu: 3.898 ± 0.491
2.021AspPhe: 2.021 ± 0.412
5.269AspGly: 5.269 ± 0.627
2.31AspHis: 2.31 ± 0.399
3.609AspIle: 3.609 ± 0.447
3.465AspLys: 3.465 ± 0.485
3.609AspLeu: 3.609 ± 0.445
1.877AspMet: 1.877 ± 0.396
2.454AspAsn: 2.454 ± 0.355
2.31AspPro: 2.31 ± 0.299
1.588AspGln: 1.588 ± 0.288
2.671AspArg: 2.671 ± 0.37
3.248AspSer: 3.248 ± 0.452
3.248AspThr: 3.248 ± 0.66
3.681AspVal: 3.681 ± 0.423
1.444AspTrp: 1.444 ± 0.296
2.165AspTyr: 2.165 ± 0.443
0.0AspXaa: 0.0 ± 0.0
Glu
7.579GluAla: 7.579 ± 1.176
1.011GluCys: 1.011 ± 0.243
2.599GluAsp: 2.599 ± 0.411
3.609GluGlu: 3.609 ± 0.629
3.465GluPhe: 3.465 ± 0.484
4.042GluGly: 4.042 ± 0.571
1.227GluHis: 1.227 ± 0.315
3.753GluIle: 3.753 ± 0.525
5.125GluLys: 5.125 ± 0.931
6.352GluLeu: 6.352 ± 0.614
1.949GluMet: 1.949 ± 0.38
3.032GluAsn: 3.032 ± 0.591
2.382GluPro: 2.382 ± 0.414
3.465GluGln: 3.465 ± 0.407
4.114GluArg: 4.114 ± 0.725
2.743GluSer: 2.743 ± 0.541
3.753GluThr: 3.753 ± 0.445
4.403GluVal: 4.403 ± 0.599
0.722GluTrp: 0.722 ± 0.179
2.021GluTyr: 2.021 ± 0.344
0.0GluXaa: 0.0 ± 0.0
Phe
3.248PheAla: 3.248 ± 0.496
0.289PheCys: 0.289 ± 0.164
2.887PheAsp: 2.887 ± 0.44
2.454PheGlu: 2.454 ± 0.437
0.938PhePhe: 0.938 ± 0.343
3.104PheGly: 3.104 ± 0.495
0.794PheHis: 0.794 ± 0.213
2.165PheIle: 2.165 ± 0.388
1.949PheLys: 1.949 ± 0.324
2.165PheLeu: 2.165 ± 0.353
1.155PheMet: 1.155 ± 0.245
1.949PheAsn: 1.949 ± 0.321
0.794PhePro: 0.794 ± 0.259
1.444PheGln: 1.444 ± 0.316
2.382PheArg: 2.382 ± 0.407
1.66PheSer: 1.66 ± 0.316
2.887PheThr: 2.887 ± 0.53
3.32PheVal: 3.32 ± 0.431
0.505PheTrp: 0.505 ± 0.226
1.083PheTyr: 1.083 ± 0.28
0.0PheXaa: 0.0 ± 0.0
Gly
5.63GlyAla: 5.63 ± 0.631
1.011GlyCys: 1.011 ± 0.283
3.753GlyAsp: 3.753 ± 0.568
4.475GlyGlu: 4.475 ± 0.585
2.743GlyPhe: 2.743 ± 0.358
6.713GlyGly: 6.713 ± 0.632
1.66GlyHis: 1.66 ± 0.399
3.898GlyIle: 3.898 ± 0.706
5.197GlyLys: 5.197 ± 0.491
5.702GlyLeu: 5.702 ± 0.567
2.093GlyMet: 2.093 ± 0.47
4.403GlyAsn: 4.403 ± 0.564
1.949GlyPro: 1.949 ± 0.348
3.248GlyGln: 3.248 ± 0.541
3.681GlyArg: 3.681 ± 0.48
4.836GlySer: 4.836 ± 0.571
4.908GlyThr: 4.908 ± 0.788
5.702GlyVal: 5.702 ± 0.811
1.227GlyTrp: 1.227 ± 0.389
2.887GlyTyr: 2.887 ± 0.384
0.0GlyXaa: 0.0 ± 0.0
His
2.238HisAla: 2.238 ± 0.421
0.361HisCys: 0.361 ± 0.153
0.794HisAsp: 0.794 ± 0.277
1.444HisGlu: 1.444 ± 0.448
0.866HisPhe: 0.866 ± 0.302
1.516HisGly: 1.516 ± 0.325
0.361HisHis: 0.361 ± 0.2
1.732HisIle: 1.732 ± 0.289
0.505HisLys: 0.505 ± 0.176
1.805HisLeu: 1.805 ± 0.42
0.722HisMet: 0.722 ± 0.236
1.299HisAsn: 1.299 ± 0.304
0.938HisPro: 0.938 ± 0.326
0.433HisGln: 0.433 ± 0.155
0.938HisArg: 0.938 ± 0.234
1.083HisSer: 1.083 ± 0.216
1.444HisThr: 1.444 ± 0.346
2.021HisVal: 2.021 ± 0.344
0.433HisTrp: 0.433 ± 0.165
0.65HisTyr: 0.65 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
5.197IleAla: 5.197 ± 0.639
0.722IleCys: 0.722 ± 0.202
3.681IleAsp: 3.681 ± 0.416
3.753IleGlu: 3.753 ± 0.654
1.516IlePhe: 1.516 ± 0.339
3.826IleGly: 3.826 ± 0.551
0.65IleHis: 0.65 ± 0.209
3.032IleIle: 3.032 ± 0.476
3.393IleLys: 3.393 ± 0.52
3.176IleLeu: 3.176 ± 0.431
1.805IleMet: 1.805 ± 0.332
3.104IleAsn: 3.104 ± 0.415
2.31IlePro: 2.31 ± 0.387
2.238IleGln: 2.238 ± 0.379
2.671IleArg: 2.671 ± 0.325
2.454IleSer: 2.454 ± 0.469
3.032IleThr: 3.032 ± 0.531
3.609IleVal: 3.609 ± 0.421
0.433IleTrp: 0.433 ± 0.222
1.444IleTyr: 1.444 ± 0.273
0.0IleXaa: 0.0 ± 0.0
Lys
5.63LysAla: 5.63 ± 1.083
0.794LysCys: 0.794 ± 0.302
3.104LysAsp: 3.104 ± 0.431
4.403LysGlu: 4.403 ± 0.678
2.887LysPhe: 2.887 ± 0.396
4.331LysGly: 4.331 ± 0.548
1.227LysHis: 1.227 ± 0.25
2.599LysIle: 2.599 ± 0.419
3.681LysLys: 3.681 ± 0.829
5.847LysLeu: 5.847 ± 0.637
1.299LysMet: 1.299 ± 0.287
1.805LysAsn: 1.805 ± 0.396
2.815LysPro: 2.815 ± 0.51
3.97LysGln: 3.97 ± 0.529
3.393LysArg: 3.393 ± 0.555
3.104LysSer: 3.104 ± 0.423
3.681LysThr: 3.681 ± 0.423
4.475LysVal: 4.475 ± 0.582
0.866LysTrp: 0.866 ± 0.236
1.732LysTyr: 1.732 ± 0.372
0.0LysXaa: 0.0 ± 0.0
Leu
6.352LeuAla: 6.352 ± 0.704
0.866LeuCys: 0.866 ± 0.234
4.836LeuAsp: 4.836 ± 0.677
6.135LeuGlu: 6.135 ± 0.691
3.104LeuPhe: 3.104 ± 0.341
5.558LeuGly: 5.558 ± 0.453
1.444LeuHis: 1.444 ± 0.362
3.104LeuIle: 3.104 ± 0.46
4.62LeuLys: 4.62 ± 0.585
5.919LeuLeu: 5.919 ± 0.505
2.671LeuMet: 2.671 ± 0.428
2.526LeuAsn: 2.526 ± 0.422
3.826LeuPro: 3.826 ± 0.472
2.887LeuGln: 2.887 ± 0.382
5.197LeuArg: 5.197 ± 0.526
4.403LeuSer: 4.403 ± 0.603
5.053LeuThr: 5.053 ± 0.494
4.042LeuVal: 4.042 ± 0.557
0.866LeuTrp: 0.866 ± 0.25
2.382LeuTyr: 2.382 ± 0.372
0.0LeuXaa: 0.0 ± 0.0
Met
2.238MetAla: 2.238 ± 0.395
0.65MetCys: 0.65 ± 0.179
1.805MetAsp: 1.805 ± 0.378
2.959MetGlu: 2.959 ± 0.695
0.938MetPhe: 0.938 ± 0.277
1.588MetGly: 1.588 ± 0.305
0.722MetHis: 0.722 ± 0.206
0.938MetIle: 0.938 ± 0.236
2.526MetLys: 2.526 ± 0.427
2.093MetLeu: 2.093 ± 0.51
1.011MetMet: 1.011 ± 0.272
1.444MetAsn: 1.444 ± 0.257
1.299MetPro: 1.299 ± 0.361
1.083MetGln: 1.083 ± 0.269
1.371MetArg: 1.371 ± 0.297
1.877MetSer: 1.877 ± 0.4
1.877MetThr: 1.877 ± 0.405
2.021MetVal: 2.021 ± 0.438
0.505MetTrp: 0.505 ± 0.21
0.577MetTyr: 0.577 ± 0.176
0.0MetXaa: 0.0 ± 0.0
Asn
3.826AsnAla: 3.826 ± 0.607
0.866AsnCys: 0.866 ± 0.255
1.877AsnAsp: 1.877 ± 0.423
2.165AsnGlu: 2.165 ± 0.342
1.299AsnPhe: 1.299 ± 0.286
4.547AsnGly: 4.547 ± 0.623
1.227AsnHis: 1.227 ± 0.383
3.537AsnIle: 3.537 ± 0.415
2.671AsnLys: 2.671 ± 0.503
3.393AsnLeu: 3.393 ± 0.456
1.516AsnMet: 1.516 ± 0.362
2.599AsnAsn: 2.599 ± 0.415
2.743AsnPro: 2.743 ± 0.369
1.877AsnGln: 1.877 ± 0.323
1.877AsnArg: 1.877 ± 0.365
2.093AsnSer: 2.093 ± 0.405
2.815AsnThr: 2.815 ± 0.466
2.815AsnVal: 2.815 ± 0.513
0.722AsnTrp: 0.722 ± 0.192
1.732AsnTyr: 1.732 ± 0.399
0.0AsnXaa: 0.0 ± 0.0
Pro
2.959ProAla: 2.959 ± 0.443
0.505ProCys: 0.505 ± 0.215
3.176ProAsp: 3.176 ± 0.573
3.826ProGlu: 3.826 ± 0.567
1.732ProPhe: 1.732 ± 0.359
2.31ProGly: 2.31 ± 0.457
0.65ProHis: 0.65 ± 0.217
2.382ProIle: 2.382 ± 0.376
2.093ProLys: 2.093 ± 0.332
2.599ProLeu: 2.599 ± 0.386
0.866ProMet: 0.866 ± 0.24
2.165ProAsn: 2.165 ± 0.539
1.877ProPro: 1.877 ± 0.365
2.021ProGln: 2.021 ± 0.681
2.021ProArg: 2.021 ± 0.43
2.671ProSer: 2.671 ± 0.46
2.454ProThr: 2.454 ± 0.458
2.526ProVal: 2.526 ± 0.532
0.794ProTrp: 0.794 ± 0.279
1.949ProTyr: 1.949 ± 0.294
0.0ProXaa: 0.0 ± 0.0
Gln
3.537GlnAla: 3.537 ± 0.561
0.938GlnCys: 0.938 ± 0.245
1.877GlnAsp: 1.877 ± 0.437
2.454GlnGlu: 2.454 ± 0.4
1.877GlnPhe: 1.877 ± 0.35
3.176GlnGly: 3.176 ± 0.62
0.866GlnHis: 0.866 ± 0.286
1.805GlnIle: 1.805 ± 0.259
1.66GlnLys: 1.66 ± 0.276
3.753GlnLeu: 3.753 ± 0.509
1.66GlnMet: 1.66 ± 0.327
1.227GlnAsn: 1.227 ± 0.295
1.877GlnPro: 1.877 ± 0.539
5.847GlnGln: 5.847 ± 4.044
2.815GlnArg: 2.815 ± 0.353
2.454GlnSer: 2.454 ± 0.386
1.516GlnThr: 1.516 ± 0.265
2.093GlnVal: 2.093 ± 0.488
0.794GlnTrp: 0.794 ± 0.298
1.227GlnTyr: 1.227 ± 0.247
0.0GlnXaa: 0.0 ± 0.0
Arg
4.259ArgAla: 4.259 ± 0.561
0.433ArgCys: 0.433 ± 0.159
2.599ArgAsp: 2.599 ± 0.508
4.042ArgGlu: 4.042 ± 0.636
2.093ArgPhe: 2.093 ± 0.386
3.97ArgGly: 3.97 ± 0.463
1.444ArgHis: 1.444 ± 0.355
3.104ArgIle: 3.104 ± 0.429
3.681ArgLys: 3.681 ± 0.557
4.114ArgLeu: 4.114 ± 0.658
1.083ArgMet: 1.083 ± 0.321
2.743ArgAsn: 2.743 ± 0.426
1.66ArgPro: 1.66 ± 0.333
1.732ArgGln: 1.732 ± 0.365
2.599ArgArg: 2.599 ± 0.508
3.032ArgSer: 3.032 ± 0.424
3.32ArgThr: 3.32 ± 0.415
4.259ArgVal: 4.259 ± 0.455
1.299ArgTrp: 1.299 ± 0.275
1.805ArgTyr: 1.805 ± 0.352
0.0ArgXaa: 0.0 ± 0.0
Ser
4.259SerAla: 4.259 ± 0.578
1.083SerCys: 1.083 ± 0.289
3.032SerAsp: 3.032 ± 0.362
2.31SerGlu: 2.31 ± 0.401
2.093SerPhe: 2.093 ± 0.364
4.908SerGly: 4.908 ± 0.758
1.155SerHis: 1.155 ± 0.272
2.021SerIle: 2.021 ± 0.447
3.826SerLys: 3.826 ± 0.598
3.826SerLeu: 3.826 ± 0.442
1.732SerMet: 1.732 ± 0.284
2.743SerAsn: 2.743 ± 0.454
2.093SerPro: 2.093 ± 0.28
1.805SerGln: 1.805 ± 0.278
3.681SerArg: 3.681 ± 0.69
3.465SerSer: 3.465 ± 0.564
2.815SerThr: 2.815 ± 0.393
3.826SerVal: 3.826 ± 0.495
0.722SerTrp: 0.722 ± 0.212
2.815SerTyr: 2.815 ± 0.411
0.0SerXaa: 0.0 ± 0.0
Thr
6.208ThrAla: 6.208 ± 0.598
0.505ThrCys: 0.505 ± 0.202
3.898ThrAsp: 3.898 ± 0.574
5.053ThrGlu: 5.053 ± 0.718
2.382ThrPhe: 2.382 ± 0.338
6.641ThrGly: 6.641 ± 0.719
1.227ThrHis: 1.227 ± 0.258
3.609ThrIle: 3.609 ± 0.573
2.382ThrLys: 2.382 ± 0.426
4.836ThrLeu: 4.836 ± 0.559
1.083ThrMet: 1.083 ± 0.268
2.599ThrAsn: 2.599 ± 0.362
3.32ThrPro: 3.32 ± 0.487
2.238ThrGln: 2.238 ± 0.376
3.032ThrArg: 3.032 ± 0.466
3.176ThrSer: 3.176 ± 0.618
4.62ThrThr: 4.62 ± 0.885
4.764ThrVal: 4.764 ± 0.961
1.155ThrTrp: 1.155 ± 0.316
1.805ThrTyr: 1.805 ± 0.354
0.0ThrXaa: 0.0 ± 0.0
Val
5.269ValAla: 5.269 ± 0.721
0.722ValCys: 0.722 ± 0.201
4.403ValAsp: 4.403 ± 0.498
4.475ValGlu: 4.475 ± 0.563
2.454ValPhe: 2.454 ± 0.371
4.908ValGly: 4.908 ± 0.643
1.588ValHis: 1.588 ± 0.418
3.898ValIle: 3.898 ± 0.654
4.547ValLys: 4.547 ± 0.682
4.259ValLeu: 4.259 ± 0.565
2.31ValMet: 2.31 ± 0.344
2.815ValAsn: 2.815 ± 0.359
2.743ValPro: 2.743 ± 0.475
2.526ValGln: 2.526 ± 0.599
3.393ValArg: 3.393 ± 0.472
3.248ValSer: 3.248 ± 0.488
6.641ValThr: 6.641 ± 0.903
6.208ValVal: 6.208 ± 0.888
1.227ValTrp: 1.227 ± 0.212
2.165ValTyr: 2.165 ± 0.403
0.0ValXaa: 0.0 ± 0.0
Trp
0.722TrpAla: 0.722 ± 0.211
0.289TrpCys: 0.289 ± 0.149
1.083TrpAsp: 1.083 ± 0.304
1.444TrpGlu: 1.444 ± 0.353
0.577TrpPhe: 0.577 ± 0.177
0.505TrpGly: 0.505 ± 0.203
0.217TrpHis: 0.217 ± 0.134
0.433TrpIle: 0.433 ± 0.186
1.155TrpLys: 1.155 ± 0.277
1.155TrpLeu: 1.155 ± 0.35
0.722TrpMet: 0.722 ± 0.207
1.083TrpAsn: 1.083 ± 0.266
0.938TrpPro: 0.938 ± 0.195
0.65TrpGln: 0.65 ± 0.285
0.65TrpArg: 0.65 ± 0.197
1.516TrpSer: 1.516 ± 0.392
0.577TrpThr: 0.577 ± 0.196
1.155TrpVal: 1.155 ± 0.308
0.361TrpTrp: 0.361 ± 0.122
1.011TrpTyr: 1.011 ± 0.3
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.238TyrAla: 2.238 ± 0.3
0.65TyrCys: 0.65 ± 0.2
2.743TyrAsp: 2.743 ± 0.454
1.732TyrGlu: 1.732 ± 0.291
1.083TyrPhe: 1.083 ± 0.331
2.671TyrGly: 2.671 ± 0.452
0.722TyrHis: 0.722 ± 0.19
1.516TyrIle: 1.516 ± 0.348
2.021TyrLys: 2.021 ± 0.401
2.599TyrLeu: 2.599 ± 0.46
0.866TyrMet: 0.866 ± 0.234
1.516TyrAsn: 1.516 ± 0.355
1.732TyrPro: 1.732 ± 0.331
0.866TyrGln: 0.866 ± 0.241
1.805TyrArg: 1.805 ± 0.324
1.66TyrSer: 1.66 ± 0.324
2.671TyrThr: 2.671 ± 0.44
2.743TyrVal: 2.743 ± 0.457
0.722TyrTrp: 0.722 ± 0.23
1.444TyrTyr: 1.444 ± 0.377
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (13855 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski