Amino acid dipepetide frequency for Pseudoalteromonas phage PH357

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.908AlaAla: 2.908 ± 0.396
0.875AlaCys: 0.875 ± 0.149
2.728AlaAsp: 2.728 ± 0.305
3.989AlaGlu: 3.989 ± 0.318
1.93AlaPhe: 1.93 ± 0.208
2.882AlaGly: 2.882 ± 0.282
0.515AlaHis: 0.515 ± 0.104
3.062AlaIle: 3.062 ± 0.252
4.272AlaLys: 4.272 ± 0.34
4.22AlaLeu: 4.22 ± 0.359
1.209AlaMet: 1.209 ± 0.167
2.702AlaAsn: 2.702 ± 0.316
1.132AlaPro: 1.132 ± 0.156
1.698AlaGln: 1.698 ± 0.192
2.187AlaArg: 2.187 ± 0.248
2.985AlaSer: 2.985 ± 0.287
2.342AlaThr: 2.342 ± 0.336
2.599AlaVal: 2.599 ± 0.251
0.489AlaTrp: 0.489 ± 0.112
2.393AlaTyr: 2.393 ± 0.202
0.0AlaXaa: 0.0 ± 0.0
Cys
0.721CysAla: 0.721 ± 0.13
0.515CysCys: 0.515 ± 0.114
0.926CysAsp: 0.926 ± 0.169
1.39CysGlu: 1.39 ± 0.21
1.029CysPhe: 1.029 ± 0.175
1.158CysGly: 1.158 ± 0.152
0.463CysHis: 0.463 ± 0.132
0.875CysIle: 0.875 ± 0.158
1.853CysLys: 1.853 ± 0.26
1.107CysLeu: 1.107 ± 0.192
0.437CysMet: 0.437 ± 0.104
1.004CysAsn: 1.004 ± 0.177
0.592CysPro: 0.592 ± 0.172
0.386CysGln: 0.386 ± 0.089
0.746CysArg: 0.746 ± 0.143
1.75CysSer: 1.75 ± 0.285
0.772CysThr: 0.772 ± 0.14
1.338CysVal: 1.338 ± 0.201
0.36CysTrp: 0.36 ± 0.093
0.875CysTyr: 0.875 ± 0.168
0.0CysXaa: 0.0 ± 0.0
Asp
2.831AspAla: 2.831 ± 0.296
1.338AspCys: 1.338 ± 0.202
3.911AspAsp: 3.911 ± 0.365
5.481AspGlu: 5.481 ± 0.349
3.808AspPhe: 3.808 ± 0.267
4.735AspGly: 4.735 ± 0.366
1.158AspHis: 1.158 ± 0.184
5.584AspIle: 5.584 ± 0.367
5.841AspLys: 5.841 ± 0.395
5.713AspLeu: 5.713 ± 0.423
1.698AspMet: 1.698 ± 0.191
5.147AspAsn: 5.147 ± 0.362
1.93AspPro: 1.93 ± 0.279
1.467AspGln: 1.467 ± 0.196
2.187AspArg: 2.187 ± 0.245
4.375AspSer: 4.375 ± 0.305
3.525AspThr: 3.525 ± 0.311
4.169AspVal: 4.169 ± 0.268
1.107AspTrp: 1.107 ± 0.171
4.194AspTyr: 4.194 ± 0.312
0.0AspXaa: 0.0 ± 0.0
Glu
4.143GluAla: 4.143 ± 0.335
1.544GluCys: 1.544 ± 0.183
7.643GluAsp: 7.643 ± 0.435
8.466GluGlu: 8.466 ± 0.553
3.448GluPhe: 3.448 ± 0.286
5.481GluGly: 5.481 ± 0.315
1.827GluHis: 1.827 ± 0.231
5.147GluIle: 5.147 ± 0.299
6.099GluLys: 6.099 ± 0.387
7.514GluLeu: 7.514 ± 0.42
2.316GluMet: 2.316 ± 0.231
4.297GluAsn: 4.297 ± 0.296
1.364GluPro: 1.364 ± 0.176
3.371GluGln: 3.371 ± 0.31
3.397GluArg: 3.397 ± 0.341
4.735GluSer: 4.735 ± 0.337
3.242GluThr: 3.242 ± 0.288
6.665GluVal: 6.665 ± 0.381
1.57GluTrp: 1.57 ± 0.261
4.658GluTyr: 4.658 ± 0.395
0.0GluXaa: 0.0 ± 0.0
Phe
2.007PheAla: 2.007 ± 0.233
0.798PheCys: 0.798 ± 0.133
3.345PheAsp: 3.345 ± 0.277
3.808PheGlu: 3.808 ± 0.319
1.415PhePhe: 1.415 ± 0.172
2.908PheGly: 2.908 ± 0.268
0.746PheHis: 0.746 ± 0.138
2.753PheIle: 2.753 ± 0.25
4.092PheLys: 4.092 ± 0.373
3.036PheLeu: 3.036 ± 0.352
1.081PheMet: 1.081 ± 0.202
2.65PheAsn: 2.65 ± 0.262
1.415PhePro: 1.415 ± 0.175
1.158PheGln: 1.158 ± 0.158
1.441PheArg: 1.441 ± 0.194
3.32PheSer: 3.32 ± 0.337
2.728PheThr: 2.728 ± 0.26
2.676PheVal: 2.676 ± 0.234
0.772PheTrp: 0.772 ± 0.15
2.11PheTyr: 2.11 ± 0.261
0.0PheXaa: 0.0 ± 0.0
Gly
2.342GlyAla: 2.342 ± 0.28
1.39GlyCys: 1.39 ± 0.191
4.58GlyAsp: 4.58 ± 0.344
5.533GlyGlu: 5.533 ± 0.333
2.959GlyPhe: 2.959 ± 0.276
3.911GlyGly: 3.911 ± 0.382
0.798GlyHis: 0.798 ± 0.148
4.04GlyIle: 4.04 ± 0.363
5.867GlyLys: 5.867 ± 0.395
4.272GlyLeu: 4.272 ± 0.331
1.107GlyMet: 1.107 ± 0.15
3.088GlyAsn: 3.088 ± 0.269
0.129GlyPro: 0.129 ± 0.081
1.673GlyGln: 1.673 ± 0.244
2.496GlyArg: 2.496 ± 0.284
3.345GlySer: 3.345 ± 0.361
2.702GlyThr: 2.702 ± 0.276
4.22GlyVal: 4.22 ± 0.333
1.158GlyTrp: 1.158 ± 0.167
3.062GlyTyr: 3.062 ± 0.256
0.0GlyXaa: 0.0 ± 0.0
His
0.695HisAla: 0.695 ± 0.118
0.54HisCys: 0.54 ± 0.124
1.107HisAsp: 1.107 ± 0.155
1.029HisGlu: 1.029 ± 0.142
0.695HisPhe: 0.695 ± 0.13
0.823HisGly: 0.823 ± 0.153
0.515HisHis: 0.515 ± 0.127
0.978HisIle: 0.978 ± 0.147
1.724HisLys: 1.724 ± 0.202
1.673HisLeu: 1.673 ± 0.217
0.335HisMet: 0.335 ± 0.094
1.415HisAsn: 1.415 ± 0.211
0.823HisPro: 0.823 ± 0.147
0.643HisGln: 0.643 ± 0.125
0.412HisArg: 0.412 ± 0.129
1.107HisSer: 1.107 ± 0.162
1.004HisThr: 1.004 ± 0.158
1.081HisVal: 1.081 ± 0.157
0.154HisTrp: 0.154 ± 0.057
1.132HisTyr: 1.132 ± 0.181
0.0HisXaa: 0.0 ± 0.0
Ile
3.525IleAla: 3.525 ± 0.322
0.978IleCys: 0.978 ± 0.148
5.224IleAsp: 5.224 ± 0.289
5.224IleGlu: 5.224 ± 0.397
2.316IlePhe: 2.316 ± 0.267
3.036IleGly: 3.036 ± 0.307
1.107IleHis: 1.107 ± 0.153
4.092IleIle: 4.092 ± 0.433
6.047IleLys: 6.047 ± 0.394
5.352IleLeu: 5.352 ± 0.376
1.107IleMet: 1.107 ± 0.199
3.551IleAsn: 3.551 ± 0.289
2.702IlePro: 2.702 ± 0.265
1.827IleGln: 1.827 ± 0.257
2.856IleArg: 2.856 ± 0.27
4.297IleSer: 4.297 ± 0.294
4.323IleThr: 4.323 ± 0.315
4.014IleVal: 4.014 ± 0.349
0.618IleTrp: 0.618 ± 0.119
2.573IleTyr: 2.573 ± 0.245
0.0IleXaa: 0.0 ± 0.0
Lys
4.683LysAla: 4.683 ± 0.344
1.081LysCys: 1.081 ± 0.198
6.819LysAsp: 6.819 ± 0.422
8.08LysGlu: 8.08 ± 0.461
3.577LysPhe: 3.577 ± 0.351
5.919LysGly: 5.919 ± 0.438
1.776LysHis: 1.776 ± 0.216
5.275LysIle: 5.275 ± 0.411
5.816LysLys: 5.816 ± 0.469
6.588LysLeu: 6.588 ± 0.482
2.316LysMet: 2.316 ± 0.247
3.5LysAsn: 3.5 ± 0.308
2.316LysPro: 2.316 ± 0.232
2.779LysGln: 2.779 ± 0.255
3.603LysArg: 3.603 ± 0.322
5.147LysSer: 5.147 ± 0.367
4.683LysThr: 4.683 ± 0.39
6.433LysVal: 6.433 ± 0.346
1.107LysTrp: 1.107 ± 0.177
3.68LysTyr: 3.68 ± 0.287
0.0LysXaa: 0.0 ± 0.0
Leu
4.143LeuAla: 4.143 ± 0.366
1.39LeuCys: 1.39 ± 0.187
5.43LeuAsp: 5.43 ± 0.416
8.389LeuGlu: 8.389 ± 0.487
3.242LeuPhe: 3.242 ± 0.352
4.503LeuGly: 4.503 ± 0.387
1.441LeuHis: 1.441 ± 0.186
5.069LeuIle: 5.069 ± 0.286
7.411LeuLys: 7.411 ± 0.421
6.536LeuLeu: 6.536 ± 0.459
2.393LeuMet: 2.393 ± 0.228
4.092LeuAsn: 4.092 ± 0.348
3.139LeuPro: 3.139 ± 0.287
2.779LeuGln: 2.779 ± 0.281
2.908LeuArg: 2.908 ± 0.279
5.841LeuSer: 5.841 ± 0.37
4.169LeuThr: 4.169 ± 0.391
4.58LeuVal: 4.58 ± 0.309
1.004LeuTrp: 1.004 ± 0.148
4.143LeuTyr: 4.143 ± 0.33
0.0LeuXaa: 0.0 ± 0.0
Met
1.235MetAla: 1.235 ± 0.23
0.489MetCys: 0.489 ± 0.131
1.132MetAsp: 1.132 ± 0.157
1.878MetGlu: 1.878 ± 0.201
1.338MetPhe: 1.338 ± 0.201
0.978MetGly: 0.978 ± 0.142
0.206MetHis: 0.206 ± 0.069
1.724MetIle: 1.724 ± 0.202
2.496MetLys: 2.496 ± 0.237
1.904MetLeu: 1.904 ± 0.216
0.515MetMet: 0.515 ± 0.111
2.033MetAsn: 2.033 ± 0.175
0.437MetPro: 0.437 ± 0.098
0.618MetGln: 0.618 ± 0.112
0.746MetArg: 0.746 ± 0.143
2.213MetSer: 2.213 ± 0.226
0.746MetThr: 0.746 ± 0.139
1.312MetVal: 1.312 ± 0.173
0.335MetTrp: 0.335 ± 0.083
1.209MetTyr: 1.209 ± 0.162
0.0MetXaa: 0.0 ± 0.0
Asn
2.136AsnAla: 2.136 ± 0.225
1.209AsnCys: 1.209 ± 0.175
2.985AsnAsp: 2.985 ± 0.3
2.65AsnGlu: 2.65 ± 0.245
2.65AsnPhe: 2.65 ± 0.288
3.603AsnGly: 3.603 ± 0.308
0.952AsnHis: 0.952 ± 0.149
3.911AsnIle: 3.911 ± 0.378
4.761AsnLys: 4.761 ± 0.391
4.863AsnLeu: 4.863 ± 0.352
1.235AsnMet: 1.235 ± 0.16
3.577AsnAsn: 3.577 ± 0.318
2.342AsnPro: 2.342 ± 0.278
1.518AsnGln: 1.518 ± 0.176
2.033AsnArg: 2.033 ± 0.221
3.731AsnSer: 3.731 ± 0.292
3.165AsnThr: 3.165 ± 0.252
3.268AsnVal: 3.268 ± 0.304
0.746AsnTrp: 0.746 ± 0.14
3.036AsnTyr: 3.036 ± 0.28
0.0AsnXaa: 0.0 ± 0.0
Pro
1.209ProAla: 1.209 ± 0.189
0.618ProCys: 0.618 ± 0.113
1.93ProAsp: 1.93 ± 0.281
3.088ProGlu: 3.088 ± 0.247
1.57ProPhe: 1.57 ± 0.175
0.051ProGly: 0.051 ± 0.034
0.566ProHis: 0.566 ± 0.127
1.827ProIle: 1.827 ± 0.236
2.316ProLys: 2.316 ± 0.26
2.367ProLeu: 2.367 ± 0.229
0.592ProMet: 0.592 ± 0.133
1.467ProAsn: 1.467 ± 0.227
0.926ProPro: 0.926 ± 0.191
1.029ProGln: 1.029 ± 0.186
0.901ProArg: 0.901 ± 0.133
2.264ProSer: 2.264 ± 0.224
1.647ProThr: 1.647 ± 0.223
2.084ProVal: 2.084 ± 0.198
0.386ProTrp: 0.386 ± 0.1
1.544ProTyr: 1.544 ± 0.193
0.0ProXaa: 0.0 ± 0.0
Gln
1.878GlnAla: 1.878 ± 0.265
0.437GlnCys: 0.437 ± 0.1
1.595GlnAsp: 1.595 ± 0.219
3.422GlnGlu: 3.422 ± 0.299
1.338GlnPhe: 1.338 ± 0.198
2.162GlnGly: 2.162 ± 0.231
0.669GlnHis: 0.669 ± 0.124
1.801GlnIle: 1.801 ± 0.213
2.496GlnLys: 2.496 ± 0.265
3.062GlnLeu: 3.062 ± 0.236
0.746GlnMet: 0.746 ± 0.122
1.364GlnAsn: 1.364 ± 0.244
0.721GlnPro: 0.721 ± 0.134
1.209GlnGln: 1.209 ± 0.18
1.673GlnArg: 1.673 ± 0.21
1.904GlnSer: 1.904 ± 0.236
1.441GlnThr: 1.441 ± 0.191
1.956GlnVal: 1.956 ± 0.187
0.257GlnTrp: 0.257 ± 0.08
1.338GlnTyr: 1.338 ± 0.197
0.0GlnXaa: 0.0 ± 0.0
Arg
1.364ArgAla: 1.364 ± 0.177
0.798ArgCys: 0.798 ± 0.196
2.934ArgAsp: 2.934 ± 0.281
3.062ArgGlu: 3.062 ± 0.323
1.776ArgPhe: 1.776 ± 0.238
2.342ArgGly: 2.342 ± 0.27
0.746ArgHis: 0.746 ± 0.135
2.29ArgIle: 2.29 ± 0.237
3.5ArgLys: 3.5 ± 0.318
3.294ArgLeu: 3.294 ± 0.3
1.055ArgMet: 1.055 ± 0.171
1.673ArgAsn: 1.673 ± 0.218
1.184ArgPro: 1.184 ± 0.18
1.338ArgGln: 1.338 ± 0.182
1.39ArgArg: 1.39 ± 0.196
2.625ArgSer: 2.625 ± 0.317
1.878ArgThr: 1.878 ± 0.218
2.753ArgVal: 2.753 ± 0.286
0.386ArgTrp: 0.386 ± 0.094
1.981ArgTyr: 1.981 ± 0.24
0.0ArgXaa: 0.0 ± 0.0
Ser
3.088SerAla: 3.088 ± 0.33
1.261SerCys: 1.261 ± 0.195
4.683SerAsp: 4.683 ± 0.321
6.047SerGlu: 6.047 ± 0.396
2.753SerPhe: 2.753 ± 0.255
3.963SerGly: 3.963 ± 0.384
1.518SerHis: 1.518 ± 0.182
3.808SerIle: 3.808 ± 0.336
5.352SerLys: 5.352 ± 0.388
5.327SerLeu: 5.327 ± 0.417
1.415SerMet: 1.415 ± 0.209
3.268SerAsn: 3.268 ± 0.26
1.595SerPro: 1.595 ± 0.179
2.213SerGln: 2.213 ± 0.254
2.393SerArg: 2.393 ± 0.243
4.117SerSer: 4.117 ± 0.517
3.242SerThr: 3.242 ± 0.266
4.915SerVal: 4.915 ± 0.331
1.055SerTrp: 1.055 ± 0.144
3.551SerTyr: 3.551 ± 0.295
0.0SerXaa: 0.0 ± 0.0
Thr
2.625ThrAla: 2.625 ± 0.274
0.643ThrCys: 0.643 ± 0.13
3.191ThrAsp: 3.191 ± 0.356
3.525ThrGlu: 3.525 ± 0.365
2.702ThrPhe: 2.702 ± 0.258
3.32ThrGly: 3.32 ± 0.361
0.798ThrHis: 0.798 ± 0.161
3.886ThrIle: 3.886 ± 0.323
4.143ThrLys: 4.143 ± 0.384
5.249ThrLeu: 5.249 ± 0.396
0.978ThrMet: 0.978 ± 0.138
2.47ThrAsn: 2.47 ± 0.269
2.162ThrPro: 2.162 ± 0.229
1.415ThrGln: 1.415 ± 0.192
1.801ThrArg: 1.801 ± 0.191
3.422ThrSer: 3.422 ± 0.347
3.32ThrThr: 3.32 ± 0.45
3.628ThrVal: 3.628 ± 0.312
0.54ThrTrp: 0.54 ± 0.125
2.367ThrTyr: 2.367 ± 0.243
0.0ThrXaa: 0.0 ± 0.0
Val
2.959ValAla: 2.959 ± 0.275
1.158ValCys: 1.158 ± 0.15
5.404ValAsp: 5.404 ± 0.43
6.407ValGlu: 6.407 ± 0.445
3.011ValPhe: 3.011 ± 0.243
3.525ValGly: 3.525 ± 0.352
1.029ValHis: 1.029 ± 0.149
4.323ValIle: 4.323 ± 0.329
6.124ValLys: 6.124 ± 0.39
4.941ValLeu: 4.941 ± 0.323
1.673ValMet: 1.673 ± 0.208
3.757ValAsn: 3.757 ± 0.29
1.904ValPro: 1.904 ± 0.224
1.981ValGln: 1.981 ± 0.249
2.11ValArg: 2.11 ± 0.249
4.426ValSer: 4.426 ± 0.324
3.577ValThr: 3.577 ± 0.308
4.889ValVal: 4.889 ± 0.322
0.823ValTrp: 0.823 ± 0.135
2.856ValTyr: 2.856 ± 0.262
0.0ValXaa: 0.0 ± 0.0
Trp
0.566TrpAla: 0.566 ± 0.157
0.335TrpCys: 0.335 ± 0.095
1.132TrpAsp: 1.132 ± 0.157
1.698TrpGlu: 1.698 ± 0.222
0.566TrpPhe: 0.566 ± 0.124
0.54TrpGly: 0.54 ± 0.115
0.232TrpHis: 0.232 ± 0.073
0.875TrpIle: 0.875 ± 0.154
1.055TrpLys: 1.055 ± 0.17
1.107TrpLeu: 1.107 ± 0.143
0.412TrpMet: 0.412 ± 0.102
0.669TrpAsn: 0.669 ± 0.13
0.103TrpPro: 0.103 ± 0.052
0.463TrpGln: 0.463 ± 0.102
0.798TrpArg: 0.798 ± 0.144
0.669TrpSer: 0.669 ± 0.127
0.669TrpThr: 0.669 ± 0.115
1.235TrpVal: 1.235 ± 0.174
0.232TrpTrp: 0.232 ± 0.069
0.489TrpTyr: 0.489 ± 0.107
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.059TyrAla: 2.059 ± 0.236
0.901TyrCys: 0.901 ± 0.175
3.5TyrAsp: 3.5 ± 0.314
3.783TyrGlu: 3.783 ± 0.321
2.136TyrPhe: 2.136 ± 0.254
2.831TyrGly: 2.831 ± 0.287
0.823TyrHis: 0.823 ± 0.133
3.448TyrIle: 3.448 ± 0.308
3.911TyrLys: 3.911 ± 0.295
4.477TyrLeu: 4.477 ± 0.329
0.901TyrMet: 0.901 ± 0.175
2.65TyrAsn: 2.65 ± 0.269
1.467TyrPro: 1.467 ± 0.17
1.904TyrGln: 1.904 ± 0.228
2.29TyrArg: 2.29 ± 0.251
3.294TyrSer: 3.294 ± 0.319
3.011TyrThr: 3.011 ± 0.257
3.062TyrVal: 3.062 ± 0.301
0.746TyrTrp: 0.746 ± 0.131
2.29TyrTyr: 2.29 ± 0.288
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 242 proteins (38862 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski