Amino acid dipepetide frequency for bacterium HR07

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.027AlaAla: 8.027 ± 0.391
0.915AlaCys: 0.915 ± 0.08
3.708AlaAsp: 3.708 ± 0.156
6.072AlaGlu: 6.072 ± 0.213
3.649AlaPhe: 3.649 ± 0.145
6.736AlaGly: 6.736 ± 0.219
2.641AlaHis: 2.641 ± 0.134
5.554AlaIle: 5.554 ± 0.179
3.883AlaLys: 3.883 ± 0.167
13.401AlaLeu: 13.401 ± 0.357
1.808AlaMet: 1.808 ± 0.106
1.96AlaAsn: 1.96 ± 0.107
4.215AlaPro: 4.215 ± 0.14
5.397AlaGln: 5.397 ± 0.215
6.981AlaArg: 6.981 ± 0.208
3.953AlaSer: 3.953 ± 0.162
4.106AlaThr: 4.106 ± 0.16
6.965AlaVal: 6.965 ± 0.203
1.095AlaTrp: 1.095 ± 0.09
2.298AlaTyr: 2.298 ± 0.135
0.0AlaXaa: 0.0 ± 0.0
Cys
0.953CysAla: 0.953 ± 0.077
0.114CysCys: 0.114 ± 0.024
0.49CysAsp: 0.49 ± 0.054
0.626CysGlu: 0.626 ± 0.053
0.387CysPhe: 0.387 ± 0.047
1.024CysGly: 1.024 ± 0.079
0.702CysHis: 0.702 ± 0.149
0.343CysIle: 0.343 ± 0.045
0.37CysLys: 0.37 ± 0.053
0.724CysLeu: 0.724 ± 0.058
0.169CysMet: 0.169 ± 0.034
0.174CysAsn: 0.174 ± 0.029
0.588CysPro: 0.588 ± 0.06
0.349CysGln: 0.349 ± 0.043
0.61CysArg: 0.61 ± 0.057
0.534CysSer: 0.534 ± 0.057
0.572CysThr: 0.572 ± 0.057
0.751CysVal: 0.751 ± 0.069
0.136CysTrp: 0.136 ± 0.025
0.3CysTyr: 0.3 ± 0.043
0.0CysXaa: 0.0 ± 0.0
Asp
4.084AspAla: 4.084 ± 0.154
0.43AspCys: 0.43 ± 0.054
1.949AspAsp: 1.949 ± 0.102
3.523AspGlu: 3.523 ± 0.142
2.173AspPhe: 2.173 ± 0.109
3.959AspGly: 3.959 ± 0.227
1.002AspHis: 1.002 ± 0.073
2.815AspIle: 2.815 ± 0.122
1.694AspLys: 1.694 ± 0.097
5.233AspLeu: 5.233 ± 0.16
0.681AspMet: 0.681 ± 0.053
0.86AspAsn: 0.86 ± 0.076
3.295AspPro: 3.295 ± 0.157
1.568AspGln: 1.568 ± 0.088
3.011AspArg: 3.011 ± 0.124
1.797AspSer: 1.797 ± 0.106
1.89AspThr: 1.89 ± 0.11
3.452AspVal: 3.452 ± 0.147
0.702AspTrp: 0.702 ± 0.069
1.269AspTyr: 1.269 ± 0.077
0.0AspXaa: 0.0 ± 0.0
Glu
6.268GluAla: 6.268 ± 0.224
0.572GluCys: 0.572 ± 0.052
2.777GluAsp: 2.777 ± 0.136
5.113GluGlu: 5.113 ± 0.195
3.093GluPhe: 3.093 ± 0.13
4.52GluGly: 4.52 ± 0.184
1.933GluHis: 1.933 ± 0.109
5.701GluIle: 5.701 ± 0.205
3.888GluLys: 3.888 ± 0.178
8.653GluLeu: 8.653 ± 0.256
1.28GluMet: 1.28 ± 0.083
1.857GluAsn: 1.857 ± 0.113
2.941GluPro: 2.941 ± 0.112
2.821GluGln: 2.821 ± 0.153
7.063GluArg: 7.063 ± 0.247
2.995GluSer: 2.995 ± 0.134
3.082GluThr: 3.082 ± 0.147
4.618GluVal: 4.618 ± 0.15
0.686GluTrp: 0.686 ± 0.069
1.944GluTyr: 1.944 ± 0.123
0.0GluXaa: 0.0 ± 0.0
Phe
3.845PheAla: 3.845 ± 0.151
0.447PheCys: 0.447 ± 0.057
2.009PheAsp: 2.009 ± 0.122
2.81PheGlu: 2.81 ± 0.116
1.906PhePhe: 1.906 ± 0.125
3.284PheGly: 3.284 ± 0.15
0.773PheHis: 0.773 ± 0.06
2.02PheIle: 2.02 ± 0.115
1.416PheLys: 1.416 ± 0.09
4.405PheLeu: 4.405 ± 0.182
0.67PheMet: 0.67 ± 0.058
1.056PheAsn: 1.056 ± 0.083
2.004PhePro: 2.004 ± 0.118
1.41PheGln: 1.41 ± 0.118
2.167PheArg: 2.167 ± 0.118
2.445PheSer: 2.445 ± 0.125
2.222PheThr: 2.222 ± 0.129
3.463PheVal: 3.463 ± 0.122
0.572PheTrp: 0.572 ± 0.053
1.356PheTyr: 1.356 ± 0.08
0.0PheXaa: 0.0 ± 0.0
Gly
7.112GlyAla: 7.112 ± 0.235
0.92GlyCys: 0.92 ± 0.074
3.518GlyAsp: 3.518 ± 0.159
5.228GlyGlu: 5.228 ± 0.189
3.082GlyPhe: 3.082 ± 0.134
5.527GlyGly: 5.527 ± 0.188
1.661GlyHis: 1.661 ± 0.103
4.732GlyIle: 4.732 ± 0.157
3.796GlyLys: 3.796 ± 0.147
8.375GlyLeu: 8.375 ± 0.248
1.704GlyMet: 1.704 ± 0.103
1.612GlyAsn: 1.612 ± 0.105
3.229GlyPro: 3.229 ± 0.144
3.224GlyGln: 3.224 ± 0.136
5.331GlyArg: 5.331 ± 0.186
3.616GlySer: 3.616 ± 0.182
4.258GlyThr: 4.258 ± 0.168
6.513GlyVal: 6.513 ± 0.218
1.171GlyTrp: 1.171 ± 0.079
2.195GlyTyr: 2.195 ± 0.12
0.0GlyXaa: 0.0 ± 0.0
His
2.135HisAla: 2.135 ± 0.115
0.278HisCys: 0.278 ± 0.035
1.035HisAsp: 1.035 ± 0.062
1.753HisGlu: 1.753 ± 0.113
0.833HisPhe: 0.833 ± 0.067
1.792HisGly: 1.792 ± 0.111
0.561HisHis: 0.561 ± 0.055
1.176HisIle: 1.176 ± 0.088
1.029HisLys: 1.029 ± 0.071
2.146HisLeu: 2.146 ± 0.13
0.349HisMet: 0.349 ± 0.04
0.615HisAsn: 0.615 ± 0.061
1.487HisPro: 1.487 ± 0.089
0.92HisGln: 0.92 ± 0.072
1.579HisArg: 1.579 ± 0.103
1.171HisSer: 1.171 ± 0.085
1.116HisThr: 1.116 ± 0.085
1.541HisVal: 1.541 ± 0.091
0.305HisTrp: 0.305 ± 0.042
0.702HisTyr: 0.702 ± 0.066
0.0HisXaa: 0.0 ± 0.0
Ile
6.948IleAla: 6.948 ± 0.202
0.686IleCys: 0.686 ± 0.069
2.783IleAsp: 2.783 ± 0.123
4.95IleGlu: 4.95 ± 0.176
2.456IlePhe: 2.456 ± 0.127
4.689IleGly: 4.689 ± 0.165
1.073IleHis: 1.073 ± 0.077
3.153IleIle: 3.153 ± 0.138
2.652IleLys: 2.652 ± 0.11
5.794IleLeu: 5.794 ± 0.227
0.893IleMet: 0.893 ± 0.079
1.459IleAsn: 1.459 ± 0.1
3.093IlePro: 3.093 ± 0.131
1.96IleGln: 1.96 ± 0.123
3.338IleArg: 3.338 ± 0.134
3.409IleSer: 3.409 ± 0.131
3.578IleThr: 3.578 ± 0.146
5.527IleVal: 5.527 ± 0.186
0.741IleTrp: 0.741 ± 0.068
1.786IleTyr: 1.786 ± 0.115
0.0IleXaa: 0.0 ± 0.0
Lys
3.812LysAla: 3.812 ± 0.157
0.316LysCys: 0.316 ± 0.042
2.14LysAsp: 2.14 ± 0.116
3.589LysGlu: 3.589 ± 0.156
1.547LysPhe: 1.547 ± 0.119
3.033LysGly: 3.033 ± 0.15
0.79LysHis: 0.79 ± 0.075
3.866LysIle: 3.866 ± 0.143
2.434LysLys: 2.434 ± 0.134
5.353LysLeu: 5.353 ± 0.166
0.779LysMet: 0.779 ± 0.067
1.4LysAsn: 1.4 ± 0.079
2.625LysPro: 2.625 ± 0.129
1.323LysGln: 1.323 ± 0.099
3.387LysArg: 3.387 ± 0.174
1.955LysSer: 1.955 ± 0.112
2.445LysThr: 2.445 ± 0.117
3.186LysVal: 3.186 ± 0.127
0.359LysTrp: 0.359 ± 0.048
1.154LysTyr: 1.154 ± 0.081
0.0LysXaa: 0.0 ± 0.0
Leu
11.299LeuAla: 11.299 ± 0.279
1.046LeuCys: 1.046 ± 0.096
5.375LeuAsp: 5.375 ± 0.167
8.152LeuGlu: 8.152 ± 0.264
4.335LeuPhe: 4.335 ± 0.191
9.034LeuGly: 9.034 ± 0.256
2.173LeuHis: 2.173 ± 0.14
6.219LeuIle: 6.219 ± 0.195
5.685LeuLys: 5.685 ± 0.186
11.49LeuLeu: 11.49 ± 0.363
2.358LeuMet: 2.358 ± 0.119
2.587LeuAsn: 2.587 ± 0.113
5.092LeuPro: 5.092 ± 0.148
4.22LeuGln: 4.22 ± 0.156
7.526LeuArg: 7.526 ± 0.218
6.398LeuSer: 6.398 ± 0.204
5.832LeuThr: 5.832 ± 0.172
8.359LeuVal: 8.359 ± 0.238
1.481LeuTrp: 1.481 ± 0.115
3.055LeuTyr: 3.055 ± 0.156
0.0LeuXaa: 0.0 ± 0.0
Met
1.851MetAla: 1.851 ± 0.103
0.114MetCys: 0.114 ± 0.025
0.692MetAsp: 0.692 ± 0.058
1.105MetGlu: 1.105 ± 0.074
0.425MetPhe: 0.425 ± 0.045
1.634MetGly: 1.634 ± 0.095
0.441MetHis: 0.441 ± 0.051
1.209MetIle: 1.209 ± 0.079
1.176MetLys: 1.176 ± 0.08
1.857MetLeu: 1.857 ± 0.104
0.408MetMet: 0.408 ± 0.065
0.594MetAsn: 0.594 ± 0.052
1.105MetPro: 1.105 ± 0.071
0.681MetGln: 0.681 ± 0.057
1.71MetArg: 1.71 ± 0.097
1.193MetSer: 1.193 ± 0.092
1.127MetThr: 1.127 ± 0.074
1.182MetVal: 1.182 ± 0.081
0.218MetTrp: 0.218 ± 0.036
0.316MetTyr: 0.316 ± 0.042
0.0MetXaa: 0.0 ± 0.0
Asn
2.026AsnAla: 2.026 ± 0.105
0.289AsnCys: 0.289 ± 0.035
0.991AsnAsp: 0.991 ± 0.083
1.318AsnGlu: 1.318 ± 0.095
1.203AsnPhe: 1.203 ± 0.082
1.792AsnGly: 1.792 ± 0.11
0.419AsnHis: 0.419 ± 0.063
1.601AsnIle: 1.601 ± 0.113
0.833AsnLys: 0.833 ± 0.072
2.815AsnLeu: 2.815 ± 0.12
0.381AsnMet: 0.381 ± 0.046
0.708AsnAsn: 0.708 ± 0.079
1.949AsnPro: 1.949 ± 0.104
0.713AsnGln: 0.713 ± 0.07
1.503AsnArg: 1.503 ± 0.085
1.024AsnSer: 1.024 ± 0.083
1.231AsnThr: 1.231 ± 0.079
1.819AsnVal: 1.819 ± 0.094
0.332AsnTrp: 0.332 ± 0.041
0.534AsnTyr: 0.534 ± 0.055
0.0AsnXaa: 0.0 ± 0.0
Pro
4.367ProAla: 4.367 ± 0.165
0.387ProCys: 0.387 ± 0.05
3.115ProAsp: 3.115 ± 0.146
3.812ProGlu: 3.812 ± 0.149
1.868ProPhe: 1.868 ± 0.108
4.171ProGly: 4.171 ± 0.166
1.383ProHis: 1.383 ± 0.089
2.794ProIle: 2.794 ± 0.129
2.625ProLys: 2.625 ± 0.14
4.514ProLeu: 4.514 ± 0.166
1.16ProMet: 1.16 ± 0.086
1.503ProAsn: 1.503 ± 0.094
2.902ProPro: 2.902 ± 0.149
2.298ProGln: 2.298 ± 0.117
2.875ProArg: 2.875 ± 0.126
2.576ProSer: 2.576 ± 0.123
3.251ProThr: 3.251 ± 0.135
3.638ProVal: 3.638 ± 0.135
0.762ProTrp: 0.762 ± 0.065
1.547ProTyr: 1.547 ± 0.092
0.0ProXaa: 0.0 ± 0.0
Gln
4.002GlnAla: 4.002 ± 0.204
0.283GlnCys: 0.283 ± 0.037
1.312GlnAsp: 1.312 ± 0.071
2.968GlnGlu: 2.968 ± 0.133
1.389GlnPhe: 1.389 ± 0.098
3.169GlnGly: 3.169 ± 0.154
0.811GlnHis: 0.811 ± 0.063
3.202GlnIle: 3.202 ± 0.145
2.14GlnLys: 2.14 ± 0.109
4.166GlnLeu: 4.166 ± 0.14
0.942GlnMet: 0.942 ± 0.086
1.073GlnAsn: 1.073 ± 0.081
1.835GlnPro: 1.835 ± 0.105
1.623GlnGln: 1.623 ± 0.107
3.327GlnArg: 3.327 ± 0.15
1.759GlnSer: 1.759 ± 0.109
2.037GlnThr: 2.037 ± 0.125
2.32GlnVal: 2.32 ± 0.106
0.441GlnTrp: 0.441 ± 0.054
0.893GlnTyr: 0.893 ± 0.074
0.0GlnXaa: 0.0 ± 0.0
Arg
7.292ArgAla: 7.292 ± 0.258
0.795ArgCys: 0.795 ± 0.073
3.643ArgAsp: 3.643 ± 0.149
6.039ArgGlu: 6.039 ± 0.212
2.941ArgPhe: 2.941 ± 0.111
4.438ArgGly: 4.438 ± 0.154
1.503ArgHis: 1.503 ± 0.088
3.801ArgIle: 3.801 ± 0.149
2.674ArgLys: 2.674 ± 0.132
7.488ArgLeu: 7.488 ± 0.233
1.323ArgMet: 1.323 ± 0.08
1.405ArgAsn: 1.405 ± 0.087
3.322ArgPro: 3.322 ± 0.151
2.679ArgGln: 2.679 ± 0.114
5.424ArgArg: 5.424 ± 0.202
3.202ArgSer: 3.202 ± 0.128
3.131ArgThr: 3.131 ± 0.142
6.202ArgVal: 6.202 ± 0.214
1.214ArgTrp: 1.214 ± 0.081
1.966ArgTyr: 1.966 ± 0.089
0.0ArgXaa: 0.0 ± 0.0
Ser
4.465SerAla: 4.465 ± 0.165
0.561SerCys: 0.561 ± 0.063
2.331SerAsp: 2.331 ± 0.127
3.621SerGlu: 3.621 ± 0.158
2.325SerPhe: 2.325 ± 0.134
4.013SerGly: 4.013 ± 0.173
0.964SerHis: 0.964 ± 0.067
2.429SerIle: 2.429 ± 0.132
1.732SerLys: 1.732 ± 0.109
5.435SerLeu: 5.435 ± 0.17
0.986SerMet: 0.986 ± 0.069
0.953SerAsn: 0.953 ± 0.064
2.941SerPro: 2.941 ± 0.171
2.091SerGln: 2.091 ± 0.101
3.006SerArg: 3.006 ± 0.13
2.592SerSer: 2.592 ± 0.153
1.999SerThr: 1.999 ± 0.087
4.204SerVal: 4.204 ± 0.166
0.806SerTrp: 0.806 ± 0.073
1.35SerTyr: 1.35 ± 0.081
0.0SerXaa: 0.0 ± 0.0
Thr
4.346ThrAla: 4.346 ± 0.152
0.572ThrCys: 0.572 ± 0.074
2.336ThrAsp: 2.336 ± 0.12
3.213ThrGlu: 3.213 ± 0.124
1.808ThrPhe: 1.808 ± 0.093
4.035ThrGly: 4.035 ± 0.154
1.171ThrHis: 1.171 ± 0.088
3.229ThrIle: 3.229 ± 0.157
2.146ThrLys: 2.146 ± 0.112
5.979ThrLeu: 5.979 ± 0.185
1.035ThrMet: 1.035 ± 0.081
1.176ThrAsn: 1.176 ± 0.085
3.354ThrPro: 3.354 ± 0.123
1.775ThrGln: 1.775 ± 0.086
2.75ThrArg: 2.75 ± 0.138
2.238ThrSer: 2.238 ± 0.122
2.592ThrThr: 2.592 ± 0.107
5.13ThrVal: 5.13 ± 0.176
0.583ThrTrp: 0.583 ± 0.058
1.389ThrTyr: 1.389 ± 0.094
0.0ThrXaa: 0.0 ± 0.0
Val
6.758ValAla: 6.758 ± 0.222
0.795ValCys: 0.795 ± 0.069
3.158ValAsp: 3.158 ± 0.143
4.89ValGlu: 4.89 ± 0.173
3.224ValPhe: 3.224 ± 0.141
6.349ValGly: 6.349 ± 0.2
1.596ValHis: 1.596 ± 0.105
5.053ValIle: 5.053 ± 0.177
3.561ValLys: 3.561 ± 0.149
9.208ValLeu: 9.208 ± 0.23
1.405ValMet: 1.405 ± 0.095
1.661ValAsn: 1.661 ± 0.1
3.692ValPro: 3.692 ± 0.128
2.979ValGln: 2.979 ± 0.141
5.696ValArg: 5.696 ± 0.18
4.139ValSer: 4.139 ± 0.151
4.471ValThr: 4.471 ± 0.148
5.538ValVal: 5.538 ± 0.204
0.871ValTrp: 0.871 ± 0.066
2.32ValTyr: 2.32 ± 0.12
0.0ValXaa: 0.0 ± 0.0
Trp
1.193TrpAla: 1.193 ± 0.083
0.12TrpCys: 0.12 ± 0.022
0.555TrpAsp: 0.555 ± 0.056
0.904TrpGlu: 0.904 ± 0.076
0.359TrpPhe: 0.359 ± 0.043
1.367TrpGly: 1.367 ± 0.097
0.31TrpHis: 0.31 ± 0.043
0.577TrpIle: 0.577 ± 0.055
0.621TrpLys: 0.621 ± 0.068
1.634TrpLeu: 1.634 ± 0.108
0.316TrpMet: 0.316 ± 0.044
0.229TrpAsn: 0.229 ± 0.037
0.561TrpPro: 0.561 ± 0.05
0.659TrpGln: 0.659 ± 0.074
1.062TrpArg: 1.062 ± 0.088
0.561TrpSer: 0.561 ± 0.058
0.506TrpThr: 0.506 ± 0.052
1.035TrpVal: 1.035 ± 0.086
0.261TrpTrp: 0.261 ± 0.039
0.24TrpTyr: 0.24 ± 0.041
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.581TyrAla: 2.581 ± 0.134
0.278TyrCys: 0.278 ± 0.04
1.47TyrAsp: 1.47 ± 0.109
2.118TyrGlu: 2.118 ± 0.113
1.28TyrPhe: 1.28 ± 0.088
2.238TyrGly: 2.238 ± 0.104
0.615TyrHis: 0.615 ± 0.061
1.492TyrIle: 1.492 ± 0.094
1.138TyrLys: 1.138 ± 0.093
3.044TyrLeu: 3.044 ± 0.135
0.387TyrMet: 0.387 ± 0.049
0.599TyrAsn: 0.599 ± 0.06
1.318TyrPro: 1.318 ± 0.094
1.002TyrGln: 1.002 ± 0.08
2.135TyrArg: 2.135 ± 0.111
1.247TyrSer: 1.247 ± 0.08
1.41TyrThr: 1.41 ± 0.093
1.928TyrVal: 1.928 ± 0.122
0.332TyrTrp: 0.332 ± 0.045
0.8TyrTyr: 0.8 ± 0.071
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 726 proteins (183638 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski