Acholeplasma oculi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Acholeplasma

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1468 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A061AGP1|A0A061AGP1_9MOLU Uncharacterized protein OS=Acholeplasma oculi OX=35623 GN=Aocu_00540 PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 10.14KK3 pKa = 10.42VYY5 pKa = 8.72TALFILAAVFILNACGSNEE24 pKa = 3.72LVIYY28 pKa = 8.67MPKK31 pKa = 10.23EE32 pKa = 4.01YY33 pKa = 9.81ISEE36 pKa = 4.17DD37 pKa = 3.15VVMAFEE43 pKa = 4.35AEE45 pKa = 4.12KK46 pKa = 10.64GIKK49 pKa = 9.86VSLRR53 pKa = 11.84YY54 pKa = 9.28FDD56 pKa = 4.35SNEE59 pKa = 3.83VLLTNAKK66 pKa = 9.77VNSYY70 pKa = 11.24DD71 pKa = 4.44LIIPSDD77 pKa = 3.78YY78 pKa = 10.95AIEE81 pKa = 4.04EE82 pKa = 4.29LATEE86 pKa = 4.74GFLKK90 pKa = 10.78KK91 pKa = 10.43LDD93 pKa = 3.35WEE95 pKa = 4.91KK96 pKa = 10.91IDD98 pKa = 4.46FEE100 pKa = 4.42SSEE103 pKa = 4.26MNEE106 pKa = 4.22SLVTTLNQLEE116 pKa = 4.26SDD118 pKa = 3.57GFDD121 pKa = 3.08FLEE124 pKa = 4.3YY125 pKa = 10.79SMPYY129 pKa = 9.23FWGTVGLIYY138 pKa = 10.92NNTIEE143 pKa = 4.45GLEE146 pKa = 4.09SDD148 pKa = 4.5VEE150 pKa = 4.28TLEE153 pKa = 4.0WAILNEE159 pKa = 4.31SKK161 pKa = 10.33YY162 pKa = 11.36SKK164 pKa = 9.99MIYY167 pKa = 10.01DD168 pKa = 4.08SSRR171 pKa = 11.84DD172 pKa = 3.25AFMAGLYY179 pKa = 11.02ANDD182 pKa = 3.7LRR184 pKa = 11.84MANPTQTNINTAKK197 pKa = 10.77DD198 pKa = 3.65FLIDD202 pKa = 4.17ANSKK206 pKa = 10.47SNASIKK212 pKa = 10.28SDD214 pKa = 3.86EE215 pKa = 4.57ILTEE219 pKa = 4.66AIGGQTPYY227 pKa = 10.68DD228 pKa = 3.46VAMVYY233 pKa = 10.49SGDD236 pKa = 3.39AVYY239 pKa = 10.52ILQEE243 pKa = 3.67TDD245 pKa = 2.69NYY247 pKa = 10.86SFYY250 pKa = 11.14VPEE253 pKa = 4.46WSNVWIDD260 pKa = 3.95GFVIPNNTKK269 pKa = 10.29HH270 pKa = 6.55EE271 pKa = 4.07DD272 pKa = 2.97WAYY275 pKa = 10.36EE276 pKa = 4.1FINFMSSYY284 pKa = 9.76EE285 pKa = 3.84ASLEE289 pKa = 3.74NTYY292 pKa = 11.74AMGYY296 pKa = 7.43TPVRR300 pKa = 11.84QDD302 pKa = 3.32VLEE305 pKa = 4.28EE306 pKa = 3.96LLADD310 pKa = 5.52EE311 pKa = 4.68EE312 pKa = 4.62FAWDD316 pKa = 4.23DD317 pKa = 4.04RR318 pKa = 11.84ISYY321 pKa = 10.44AFTVPVVNFEE331 pKa = 4.19FYY333 pKa = 10.47RR334 pKa = 11.84YY335 pKa = 9.54NATLKK340 pKa = 11.2SMIDD344 pKa = 3.73DD345 pKa = 3.24AWEE348 pKa = 3.77LVILSNN354 pKa = 5.04

Molecular weight: 40.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A061AF12|A0A061AF12_9MOLU Glutamine--tRNA ligase OS=Acholeplasma oculi OX=35623 GN=glnS PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.95QPSKK9 pKa = 9.64IKK11 pKa = 10.09HH12 pKa = 4.75QRR14 pKa = 11.84THH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.93VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84HH40 pKa = 4.39VLTVV44 pKa = 3.21

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1468

0

1468

484631

37

6297

330.1

37.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.322 ± 0.059

0.408 ± 0.016

5.848 ± 0.048

6.491 ± 0.06

5.016 ± 0.048

5.879 ± 0.08

2.115 ± 0.033

9.109 ± 0.068

7.851 ± 0.102

10.193 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.587 ± 0.03

5.405 ± 0.051

3.064 ± 0.033

3.398 ± 0.038

3.36 ± 0.044

6.26 ± 0.05

5.646 ± 0.098

6.596 ± 0.067

0.752 ± 0.02

4.701 ± 0.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski