Amino acid dipepetide frequency for Rhodobacteraceae bacterium CCMM004

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	24.262AlaAla: 24.262 ± 0.291	1.191AlaCys: 1.191 ± 0.031	8.598AlaAsp: 8.598 ± 0.098	9.396AlaGlu: 9.396 ± 0.123	4.564AlaPhe: 4.564 ± 0.063	12.184AlaGly: 12.184 ± 0.116	2.691AlaHis: 2.691 ± 0.05	5.447AlaIle: 5.447 ± 0.07	2.579AlaLys: 2.579 ± 0.055	16.072AlaLeu: 16.072 ± 0.149	3.983AlaMet: 3.983 ± 0.049	2.242AlaAsn: 2.242 ± 0.048	7.69AlaPro: 7.69 ± 0.14	4.429AlaGln: 4.429 ± 0.06	11.291AlaArg: 11.291 ± 0.115	4.784AlaSer: 4.784 ± 0.057	6.424AlaThr: 6.424 ± 0.071	10.645AlaVal: 10.645 ± 0.116	1.887AlaTrp: 1.887 ± 0.041	2.641AlaTyr: 2.641 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	1.194CysAla: 1.194 ± 0.031	0.113CysCys: 0.113 ± 0.01	0.624CysAsp: 0.624 ± 0.023	0.38CysGlu: 0.38 ± 0.018	0.286CysPhe: 0.286 ± 0.016	0.981CysGly: 0.981 ± 0.029	0.28CysHis: 0.28 ± 0.017	0.384CysIle: 0.384 ± 0.019	0.132CysLys: 0.132 ± 0.009	0.803CysLeu: 0.803 ± 0.025	0.136CysMet: 0.136 ± 0.01	0.192CysAsn: 0.192 ± 0.012	0.54CysPro: 0.54 ± 0.02	0.2CysGln: 0.2 ± 0.012	0.683CysArg: 0.683 ± 0.025	0.343CysSer: 0.343 ± 0.015	0.461CysThr: 0.461 ± 0.021	0.635CysVal: 0.635 ± 0.023	0.124CysTrp: 0.124 ± 0.01	0.206CysTyr: 0.206 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	8.702AspAla: 8.702 ± 0.099	0.528AspCys: 0.528 ± 0.02	3.917AspAsp: 3.917 ± 0.084	3.318AspGlu: 3.318 ± 0.047	2.092AspPhe: 2.092 ± 0.039	6.424AspGly: 6.424 ± 0.088	1.345AspHis: 1.345 ± 0.031	2.708AspIle: 2.708 ± 0.05	1.261AspLys: 1.261 ± 0.036	7.171AspLeu: 7.171 ± 0.085	1.532AspMet: 1.532 ± 0.033	1.027AspAsn: 1.027 ± 0.03	4.537AspPro: 4.537 ± 0.065	1.443AspGln: 1.443 ± 0.032	5.79AspArg: 5.79 ± 0.078	1.851AspSer: 1.851 ± 0.037	3.263AspThr: 3.263 ± 0.058	4.383AspVal: 4.383 ± 0.06	1.349AspTrp: 1.349 ± 0.029	1.491AspTyr: 1.491 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	8.573GluAla: 8.573 ± 0.125	0.272GluCys: 0.272 ± 0.013	3.289GluAsp: 3.289 ± 0.056	3.026GluGlu: 3.026 ± 0.067	1.521GluPhe: 1.521 ± 0.039	5.18GluGly: 5.18 ± 0.074	0.955GluHis: 0.955 ± 0.027	3.335GluIle: 3.335 ± 0.045	1.384GluLys: 1.384 ± 0.04	4.237GluLeu: 4.237 ± 0.058	1.776GluMet: 1.776 ± 0.046	1.258GluAsn: 1.258 ± 0.034	2.411GluPro: 2.411 ± 0.047	1.346GluGln: 1.346 ± 0.031	4.594GluArg: 4.594 ± 0.071	1.862GluSer: 1.862 ± 0.031	3.872GluThr: 3.872 ± 0.066	4.631GluVal: 4.631 ± 0.065	0.667GluTrp: 0.667 ± 0.024	0.791GluTyr: 0.791 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.73PheAla: 4.73 ± 0.072	0.404PheCys: 0.404 ± 0.018	2.879PheAsp: 2.879 ± 0.052	1.82PheGlu: 1.82 ± 0.041	1.267PhePhe: 1.267 ± 0.041	3.422PheGly: 3.422 ± 0.06	0.698PheHis: 0.698 ± 0.024	1.245PheIle: 1.245 ± 0.032	0.662PheLys: 0.662 ± 0.023	3.232PheLeu: 3.232 ± 0.064	0.672PheMet: 0.672 ± 0.024	0.797PheAsn: 0.797 ± 0.025	1.513PhePro: 1.513 ± 0.038	0.95PheGln: 0.95 ± 0.029	2.238PheArg: 2.238 ± 0.046	1.753PheSer: 1.753 ± 0.037	1.81PheThr: 1.81 ± 0.037	2.737PheVal: 2.737 ± 0.047	0.549PheTrp: 0.549 ± 0.023	0.86PheTyr: 0.86 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	12.083GlyAla: 12.083 ± 0.146	0.91GlyCys: 0.91 ± 0.028	5.248GlyAsp: 5.248 ± 0.088	4.377GlyGlu: 4.377 ± 0.062	3.317GlyPhe: 3.317 ± 0.056	8.609GlyGly: 8.609 ± 0.108	2.113GlyHis: 2.113 ± 0.042	4.149GlyIle: 4.149 ± 0.067	2.231GlyLys: 2.231 ± 0.05	9.554GlyLeu: 9.554 ± 0.097	2.458GlyMet: 2.458 ± 0.045	1.685GlyAsn: 1.685 ± 0.046	4.706GlyPro: 4.706 ± 0.073	2.812GlyGln: 2.812 ± 0.048	7.656GlyArg: 7.656 ± 0.075	3.676GlySer: 3.676 ± 0.06	5.113GlyThr: 5.113 ± 0.066	6.805GlyVal: 6.805 ± 0.081	1.598GlyTrp: 1.598 ± 0.042	2.265GlyTyr: 2.265 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.573HisAla: 2.573 ± 0.049	0.245HisCys: 0.245 ± 0.014	1.365HisAsp: 1.365 ± 0.036	0.965HisGlu: 0.965 ± 0.028	0.695HisPhe: 0.695 ± 0.02	2.092HisGly: 2.092 ± 0.044	0.554HisHis: 0.554 ± 0.026	0.819HisIle: 0.819 ± 0.026	0.369HisLys: 0.369 ± 0.018	2.268HisLeu: 2.268 ± 0.044	0.506HisMet: 0.506 ± 0.019	0.366HisAsn: 0.366 ± 0.018	1.522HisPro: 1.522 ± 0.038	0.484HisGln: 0.484 ± 0.018	1.671HisArg: 1.671 ± 0.041	0.778HisSer: 0.778 ± 0.027	0.866HisThr: 0.866 ± 0.029	1.589HisVal: 1.589 ± 0.032	0.35HisTrp: 0.35 ± 0.017	0.537HisTyr: 0.537 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.048IleAla: 7.048 ± 0.08	0.508IleCys: 0.508 ± 0.021	3.282IleAsp: 3.282 ± 0.053	2.767IleGlu: 2.767 ± 0.051	1.474IlePhe: 1.474 ± 0.04	4.336IleGly: 4.336 ± 0.067	0.8IleHis: 0.8 ± 0.021	1.263IleIle: 1.263 ± 0.038	0.911IleLys: 0.911 ± 0.028	4.506IleLeu: 4.506 ± 0.069	0.758IleMet: 0.758 ± 0.028	0.952IleAsn: 0.952 ± 0.032	2.082IlePro: 2.082 ± 0.042	0.882IleGln: 0.882 ± 0.025	2.911IleArg: 2.911 ± 0.05	2.138IleSer: 2.138 ± 0.043	2.112IleThr: 2.112 ± 0.036	3.84IleVal: 3.84 ± 0.061	0.628IleTrp: 0.628 ± 0.025	0.955IleTyr: 0.955 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	2.731LysAla: 2.731 ± 0.054	0.136LysCys: 0.136 ± 0.011	1.247LysAsp: 1.247 ± 0.033	1.031LysGlu: 1.031 ± 0.034	0.62LysPhe: 0.62 ± 0.023	1.899LysGly: 1.899 ± 0.047	0.402LysHis: 0.402 ± 0.018	1.11LysIle: 1.11 ± 0.03	0.688LysLys: 0.688 ± 0.03	1.98LysLeu: 1.98 ± 0.049	0.597LysMet: 0.597 ± 0.022	0.482LysAsn: 0.482 ± 0.021	1.2LysPro: 1.2 ± 0.034	0.517LysGln: 0.517 ± 0.02	1.679LysArg: 1.679 ± 0.043	1.24LysSer: 1.24 ± 0.033	1.381LysThr: 1.381 ± 0.035	1.734LysVal: 1.734 ± 0.045	0.283LysTrp: 0.283 ± 0.015	0.462LysTyr: 0.462 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	14.892LeuAla: 14.892 ± 0.135	0.979LeuCys: 0.979 ± 0.03	6.882LeuAsp: 6.882 ± 0.071	4.989LeuGlu: 4.989 ± 0.066	3.342LeuPhe: 3.342 ± 0.059	8.989LeuGly: 8.989 ± 0.107	1.964LeuHis: 1.964 ± 0.037	4.964LeuIle: 4.964 ± 0.068	2.312LeuLys: 2.312 ± 0.05	8.503LeuLeu: 8.503 ± 0.105	2.387LeuMet: 2.387 ± 0.042	2.196LeuAsn: 2.196 ± 0.047	5.815LeuPro: 5.815 ± 0.077	2.101LeuGln: 2.101 ± 0.039	7.725LeuArg: 7.725 ± 0.088	6.15LeuSer: 6.15 ± 0.081	6.437LeuThr: 6.437 ± 0.079	6.823LeuVal: 6.823 ± 0.084	1.517LeuTrp: 1.517 ± 0.04	2.048LeuTyr: 2.048 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	3.705MetAla: 3.705 ± 0.051	0.166MetCys: 0.166 ± 0.01	1.351MetAsp: 1.351 ± 0.033	1.221MetGlu: 1.221 ± 0.033	0.707MetPhe: 0.707 ± 0.024	2.17MetGly: 2.17 ± 0.039	0.417MetHis: 0.417 ± 0.02	1.259MetIle: 1.259 ± 0.032	0.696MetLys: 0.696 ± 0.022	2.313MetLeu: 2.313 ± 0.041	0.643MetMet: 0.643 ± 0.025	0.617MetAsn: 0.617 ± 0.019	1.471MetPro: 1.471 ± 0.031	0.789MetGln: 0.789 ± 0.023	1.896MetArg: 1.896 ± 0.039	1.465MetSer: 1.465 ± 0.033	2.053MetThr: 2.053 ± 0.04	1.747MetVal: 1.747 ± 0.035	0.262MetTrp: 0.262 ± 0.015	0.304MetTyr: 0.304 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.678AsnAla: 2.678 ± 0.05	0.199AsnCys: 0.199 ± 0.011	1.186AsnAsp: 1.186 ± 0.042	0.814AsnGlu: 0.814 ± 0.027	0.765AsnPhe: 0.765 ± 0.024	1.819AsnGly: 1.819 ± 0.039	0.426AsnHis: 0.426 ± 0.017	0.924AsnIle: 0.924 ± 0.024	0.401AsnLys: 0.401 ± 0.018	2.067AsnLeu: 2.067 ± 0.045	0.51AsnMet: 0.51 ± 0.022	0.482AsnAsn: 0.482 ± 0.023	1.581AsnPro: 1.581 ± 0.035	0.485AsnGln: 0.485 ± 0.02	1.523AsnArg: 1.523 ± 0.036	0.775AsnSer: 0.775 ± 0.027	0.935AsnThr: 0.935 ± 0.028	1.6AsnVal: 1.6 ± 0.037	0.324AsnTrp: 0.324 ± 0.019	0.543AsnTyr: 0.543 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	7.399ProAla: 7.399 ± 0.118	0.382ProCys: 0.382 ± 0.017	4.504ProAsp: 4.504 ± 0.07	4.16ProGlu: 4.16 ± 0.062	1.928ProPhe: 1.928 ± 0.039	5.628ProGly: 5.628 ± 0.077	1.205ProHis: 1.205 ± 0.032	1.99ProIle: 1.99 ± 0.04	1.193ProLys: 1.193 ± 0.031	5.081ProLeu: 5.081 ± 0.057	1.378ProMet: 1.378 ± 0.036	1.123ProAsn: 1.123 ± 0.031	3.401ProPro: 3.401 ± 0.09	1.693ProGln: 1.693 ± 0.035	3.761ProArg: 3.761 ± 0.063	2.393ProSer: 2.393 ± 0.042	2.57ProThr: 2.57 ± 0.049	4.604ProVal: 4.604 ± 0.068	0.836ProTrp: 0.836 ± 0.026	1.154ProTyr: 1.154 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.754GlnAla: 3.754 ± 0.052	0.188GlnCys: 0.188 ± 0.012	1.516GlnAsp: 1.516 ± 0.036	1.351GlnGlu: 1.351 ± 0.033	0.869GlnPhe: 0.869 ± 0.025	2.419GlnGly: 2.419 ± 0.046	0.497GlnHis: 0.497 ± 0.019	1.608GlnIle: 1.608 ± 0.038	0.71GlnLys: 0.71 ± 0.024	2.128GlnLeu: 2.128 ± 0.043	0.863GlnMet: 0.863 ± 0.027	0.643GlnAsn: 0.643 ± 0.022	1.424GlnPro: 1.424 ± 0.034	0.79GlnGln: 0.79 ± 0.03	2.093GlnArg: 2.093 ± 0.043	1.436GlnSer: 1.436 ± 0.035	1.65GlnThr: 1.65 ± 0.038	2.193GlnVal: 2.193 ± 0.047	0.35GlnTrp: 0.35 ± 0.015	0.469GlnTyr: 0.469 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	10.628ArgAla: 10.628 ± 0.129	0.581ArgCys: 0.581 ± 0.024	5.028ArgAsp: 5.028 ± 0.068	3.841ArgGlu: 3.841 ± 0.059	2.816ArgPhe: 2.816 ± 0.052	5.791ArgGly: 5.791 ± 0.075	1.878ArgHis: 1.878 ± 0.045	3.948ArgIle: 3.948 ± 0.06	1.696ArgLys: 1.696 ± 0.035	8.744ArgLeu: 8.744 ± 0.09	2.057ArgMet: 2.057 ± 0.035	1.515ArgAsn: 1.515 ± 0.034	4.459ArgPro: 4.459 ± 0.07	2.381ArgGln: 2.381 ± 0.044	6.968ArgArg: 6.968 ± 0.099	3.302ArgSer: 3.302 ± 0.054	3.674ArgThr: 3.674 ± 0.054	5.489ArgVal: 5.489 ± 0.075	1.22ArgTrp: 1.22 ± 0.035	1.711ArgTyr: 1.711 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	5.456SerAla: 5.456 ± 0.06	0.364SerCys: 0.364 ± 0.017	2.944SerAsp: 2.944 ± 0.05	2.324SerGlu: 2.324 ± 0.049	1.85SerPhe: 1.85 ± 0.043	4.751SerGly: 4.751 ± 0.078	0.985SerHis: 0.985 ± 0.032	1.806SerIle: 1.806 ± 0.04	0.981SerLys: 0.981 ± 0.028	4.326SerLeu: 4.326 ± 0.057	1.097SerMet: 1.097 ± 0.03	0.975SerAsn: 0.975 ± 0.027	2.304SerPro: 2.304 ± 0.045	1.244SerGln: 1.244 ± 0.029	3.034SerArg: 3.034 ± 0.054	1.914SerSer: 1.914 ± 0.041	2.121SerThr: 2.121 ± 0.046	3.446SerVal: 3.446 ± 0.053	0.61SerTrp: 0.61 ± 0.023	1.12SerTyr: 1.12 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	7.322ThrAla: 7.322 ± 0.087	0.471ThrCys: 0.471 ± 0.022	3.341ThrAsp: 3.341 ± 0.048	2.761ThrGlu: 2.761 ± 0.049	1.943ThrPhe: 1.943 ± 0.038	5.409ThrGly: 5.409 ± 0.065	1.1ThrHis: 1.1 ± 0.026	2.166ThrIle: 2.166 ± 0.046	0.911ThrLys: 0.911 ± 0.03	6.245ThrLeu: 6.245 ± 0.077	1.158ThrMet: 1.158 ± 0.029	0.941ThrAsn: 0.941 ± 0.026	3.605ThrPro: 3.605 ± 0.056	1.309ThrGln: 1.309 ± 0.031	3.682ThrArg: 3.682 ± 0.058	2.176ThrSer: 2.176 ± 0.042	2.491ThrThr: 2.491 ± 0.045	4.811ThrVal: 4.811 ± 0.061	0.792ThrTrp: 0.792 ± 0.028	1.162ThrTyr: 1.162 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	10.945ValAla: 10.945 ± 0.107	0.698ValCys: 0.698 ± 0.023	4.411ValAsp: 4.411 ± 0.062	4.614ValGlu: 4.614 ± 0.059	2.82ValPhe: 2.82 ± 0.052	6.024ValGly: 6.024 ± 0.079	1.458ValHis: 1.458 ± 0.032	3.54ValIle: 3.54 ± 0.066	1.561ValLys: 1.561 ± 0.04	7.906ValLeu: 7.906 ± 0.093	1.898ValMet: 1.898 ± 0.044	1.664ValAsn: 1.664 ± 0.038	4.249ValPro: 4.249 ± 0.062	1.973ValGln: 1.973 ± 0.041	5.335ValArg: 5.335 ± 0.068	3.855ValSer: 3.855 ± 0.059	4.592ValThr: 4.592 ± 0.06	6.153ValVal: 6.153 ± 0.084	1.162ValTrp: 1.162 ± 0.03	1.539ValTyr: 1.539 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.646TrpAla: 1.646 ± 0.042	0.143TrpCys: 0.143 ± 0.012	0.927TrpAsp: 0.927 ± 0.03	0.736TrpGlu: 0.736 ± 0.024	0.572TrpPhe: 0.572 ± 0.025	1.128TrpGly: 1.128 ± 0.033	0.369TrpHis: 0.369 ± 0.019	0.747TrpIle: 0.747 ± 0.024	0.324TrpLys: 0.324 ± 0.015	1.69TrpLeu: 1.69 ± 0.041	0.406TrpMet: 0.406 ± 0.016	0.371TrpAsn: 0.371 ± 0.015	0.834TrpPro: 0.834 ± 0.029	0.568TrpGln: 0.568 ± 0.023	1.395TrpArg: 1.395 ± 0.035	0.827TrpSer: 0.827 ± 0.027	0.95TrpThr: 0.95 ± 0.032	0.965TrpVal: 0.965 ± 0.028	0.269TrpTrp: 0.269 ± 0.016	0.272TrpTyr: 0.272 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.676TyrAla: 2.676 ± 0.044	0.221TyrCys: 0.221 ± 0.012	1.596TyrAsp: 1.596 ± 0.037	1.089TyrGlu: 1.089 ± 0.036	0.791TyrPhe: 0.791 ± 0.025	2.112TyrGly: 2.112 ± 0.041	0.474TyrHis: 0.474 ± 0.019	0.742TyrIle: 0.742 ± 0.025	0.417TyrLys: 0.417 ± 0.02	2.189TyrLeu: 2.189 ± 0.042	0.437TyrMet: 0.437 ± 0.021	0.508TyrAsn: 0.508 ± 0.018	1.038TyrPro: 1.038 ± 0.03	0.554TyrGln: 0.554 ± 0.024	1.8TyrArg: 1.8 ± 0.043	0.93TyrSer: 0.93 ± 0.028	1.023TyrThr: 1.023 ± 0.033	1.57TyrVal: 1.57 ± 0.031	0.363TyrTrp: 0.363 ± 0.018	0.53TyrTyr: 0.53 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4132 proteins (1310337 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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