Coprinopsis marcescibilis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Psathyrellaceae; Coprinopsis

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14526 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C3KL82|A0A5C3KL82_9AGAR Uncharacterized protein OS=Coprinopsis marcescibilis OX=230819 GN=FA15DRAFT_658624 PE=4 SV=1
MM1 pKa = 7.5YY2 pKa = 8.44STLLAVALFVAPVFSAYY19 pKa = 10.17AVNNPDD25 pKa = 4.84LVQCQTARR33 pKa = 11.84ISWPHH38 pKa = 4.58TTYY41 pKa = 10.45QYY43 pKa = 11.8NVIAVAADD51 pKa = 4.09EE52 pKa = 4.69PCGEE56 pKa = 4.31PLVDD60 pKa = 4.97IGDD63 pKa = 3.91LTEE66 pKa = 6.08SFIDD70 pKa = 3.43WQVNIPAGTVVQLSVADD87 pKa = 3.99SGDD90 pKa = 3.63AEE92 pKa = 4.2SWTRR96 pKa = 11.84NLTVAASDD104 pKa = 5.11DD105 pKa = 4.36SSCLPGFIASSSAAAASRR123 pKa = 11.84PTSAPARR130 pKa = 11.84NLAGSSAAPRR140 pKa = 11.84ASPSAGSDD148 pKa = 3.14DD149 pKa = 4.52SAFDD153 pKa = 4.46PIGAVNAADD162 pKa = 4.6LPFSAAPSTRR172 pKa = 11.84QLGVPALALGALAALAALALL192 pKa = 4.24

Molecular weight: 19.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C3L8P5|A0A5C3L8P5_9AGAR Cation/H+ exchanger OS=Coprinopsis marcescibilis OX=230819 GN=FA15DRAFT_691488 PE=4 SV=1
MM1 pKa = 7.78PFMTRR6 pKa = 11.84RR7 pKa = 11.84SRR9 pKa = 11.84ATTTHH14 pKa = 5.69VPASLPSKK22 pKa = 10.29GLGIFRR28 pKa = 11.84QFKK31 pKa = 10.27LRR33 pKa = 11.84RR34 pKa = 11.84NPNRR38 pKa = 11.84VAGGHH43 pKa = 6.2KK44 pKa = 10.04AALSNPHH51 pKa = 5.42TTRR54 pKa = 11.84QGRR57 pKa = 11.84KK58 pKa = 7.96HH59 pKa = 5.91AKK61 pKa = 9.55KK62 pKa = 9.55EE63 pKa = 3.67LRR65 pKa = 11.84RR66 pKa = 11.84MGRR69 pKa = 11.84GNATHH74 pKa = 6.5VPILTKK80 pKa = 10.17IKK82 pKa = 9.72RR83 pKa = 11.84ALGITSSRR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84HH94 pKa = 4.18TQRR97 pKa = 11.84RR98 pKa = 11.84TVIVV102 pKa = 3.42

Molecular weight: 11.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14526

0

14526

6217334

49

4884

428.0

47.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.191 ± 0.017

1.265 ± 0.008

5.469 ± 0.014

5.905 ± 0.023

3.767 ± 0.014

6.56 ± 0.022

2.539 ± 0.01

4.961 ± 0.016

4.681 ± 0.02

9.335 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.965 ± 0.008

3.727 ± 0.011

6.444 ± 0.027

3.915 ± 0.014

6.135 ± 0.019

8.726 ± 0.029

5.995 ± 0.014

6.333 ± 0.016

1.414 ± 0.007

2.672 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski