Pseudobythopirellula maris

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Lacipirellulaceae; Pseudobythopirellula

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3893 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C5ZTJ2|A0A5C5ZTJ2_9BACT Bifunctional purine biosynthesis protein PurH OS=Pseudobythopirellula maris OX=2527991 GN=purH PE=3 SV=1
MM1 pKa = 7.34LVDD4 pKa = 3.87TGMIDD9 pKa = 3.2TPRR12 pKa = 11.84TVRR15 pKa = 11.84LQSPSILTALAFTLLLAAPALGAKK39 pKa = 9.55YY40 pKa = 9.09PAYY43 pKa = 10.72VDD45 pKa = 3.71GSFTFGDD52 pKa = 3.93EE53 pKa = 4.09FAEE56 pKa = 4.31APYY59 pKa = 10.71GLEE62 pKa = 4.12STFSLSSNPNATKK75 pKa = 10.05TIYY78 pKa = 10.84LDD80 pKa = 3.28FTGFHH85 pKa = 6.44SVGNSWGHH93 pKa = 5.97NIVFPAYY100 pKa = 10.32DD101 pKa = 3.94LDD103 pKa = 4.8GDD105 pKa = 4.07SGSFSDD111 pKa = 4.66EE112 pKa = 3.77EE113 pKa = 4.76LIEE116 pKa = 4.12IQQIFQNVAEE126 pKa = 5.03DD127 pKa = 3.94YY128 pKa = 11.06IPFNVNVTTADD139 pKa = 3.44PGATALRR146 pKa = 11.84KK147 pKa = 10.21LGDD150 pKa = 4.09GDD152 pKa = 4.02THH154 pKa = 6.42WGMRR158 pKa = 11.84VVMTQATAGFGVGIGGDD175 pKa = 3.55SGSSRR180 pKa = 11.84FDD182 pKa = 3.55DD183 pKa = 4.76AADD186 pKa = 3.54NPVFTFNKK194 pKa = 9.04GARR197 pKa = 11.84KK198 pKa = 9.76GGLTASHH205 pKa = 6.24EE206 pKa = 4.41VGHH209 pKa = 5.96TFGLAHH215 pKa = 7.43DD216 pKa = 4.47SYY218 pKa = 11.73YY219 pKa = 10.87DD220 pKa = 3.53QEE222 pKa = 4.4YY223 pKa = 10.65HH224 pKa = 6.98PGTGGYY230 pKa = 10.41GRR232 pKa = 11.84TSWGPLMGAPFDD244 pKa = 4.36ASITQWSNGDD254 pKa = 3.5YY255 pKa = 10.67AGSSNTFDD263 pKa = 4.38DD264 pKa = 4.3YY265 pKa = 12.18SFITSKK271 pKa = 10.99GFGFRR276 pKa = 11.84EE277 pKa = 3.65DD278 pKa = 4.87DD279 pKa = 3.95YY280 pKa = 11.66LNSLADD286 pKa = 3.53PHH288 pKa = 8.11LLEE291 pKa = 6.09SIDD294 pKa = 4.15GEE296 pKa = 3.95ISEE299 pKa = 4.28WGIIEE304 pKa = 3.96EE305 pKa = 4.59RR306 pKa = 11.84YY307 pKa = 10.37DD308 pKa = 3.37RR309 pKa = 11.84DD310 pKa = 3.42YY311 pKa = 11.66FRR313 pKa = 11.84FTTTTGEE320 pKa = 3.86VSFDD324 pKa = 3.62VNGFAQDD331 pKa = 3.61PNIDD335 pKa = 3.92VGVVIYY341 pKa = 10.61DD342 pKa = 3.43SLGEE346 pKa = 4.21VVVEE350 pKa = 4.63LDD352 pKa = 3.74PSVTPNINYY361 pKa = 10.09DD362 pKa = 3.79LFLTAGDD369 pKa = 3.56YY370 pKa = 10.92TFFVDD375 pKa = 5.87GVDD378 pKa = 3.46NPGRR382 pKa = 11.84YY383 pKa = 8.89SDD385 pKa = 3.77YY386 pKa = 11.25GSLGFYY392 pKa = 10.08DD393 pKa = 3.77IQIGLPIAGDD403 pKa = 3.84LNSDD407 pKa = 3.9GFVDD411 pKa = 3.64TDD413 pKa = 3.15DD414 pKa = 3.79WLLLVAGAEE423 pKa = 4.24TDD425 pKa = 3.59LSGLSAEE432 pKa = 4.46EE433 pKa = 4.03AFLAGDD439 pKa = 4.03LNGDD443 pKa = 4.23GLNDD447 pKa = 3.36IYY449 pKa = 11.56DD450 pKa = 3.83FGLFKK455 pKa = 10.96DD456 pKa = 4.46AFTSAHH462 pKa = 5.89GAAEE466 pKa = 4.57FAALLAPEE474 pKa = 4.14PAGLGLATAMVLMLAGYY491 pKa = 7.33VRR493 pKa = 11.84CGVRR497 pKa = 11.84TNN499 pKa = 3.28

Molecular weight: 53.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C5ZRC2|A0A5C5ZRC2_9BACT PSP1 C-terminal domain-containing protein OS=Pseudobythopirellula maris OX=2527991 GN=Mal64_04850 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84LANKK6 pKa = 9.79HH7 pKa = 5.74AMITGAASGIGRR19 pKa = 11.84AIAMRR24 pKa = 11.84LASRR28 pKa = 11.84MTHH31 pKa = 5.65LTLVDD36 pKa = 3.38IDD38 pKa = 3.77AAGLEE43 pKa = 4.31RR44 pKa = 11.84TARR47 pKa = 11.84EE48 pKa = 3.91ARR50 pKa = 11.84EE51 pKa = 3.78LGAAVDD57 pKa = 3.73SQRR60 pKa = 11.84RR61 pKa = 11.84DD62 pKa = 3.0LTLRR66 pKa = 11.84DD67 pKa = 3.64EE68 pKa = 4.58VDD70 pKa = 3.06ATVQEE75 pKa = 4.75SLDD78 pKa = 3.49QSAGVDD84 pKa = 3.52LLVNNAGVTYY94 pKa = 10.19HH95 pKa = 6.83GPTHH99 pKa = 7.05AMPAGDD105 pKa = 4.86LEE107 pKa = 4.8RR108 pKa = 11.84LMSLNLLAHH117 pKa = 5.88VRR119 pKa = 11.84LTHH122 pKa = 6.59GLLPSLLARR131 pKa = 11.84PEE133 pKa = 3.78AHH135 pKa = 6.31VLNVCSVLGLAGMPRR150 pKa = 11.84VSAYY154 pKa = 10.45CAGKK158 pKa = 9.16FAMVGFSEE166 pKa = 4.48SLRR169 pKa = 11.84SEE171 pKa = 4.17YY172 pKa = 10.96GRR174 pKa = 11.84VGLGVTAFCPGFVRR188 pKa = 11.84TEE190 pKa = 4.13LFGSAPLPEE199 pKa = 4.84GQSKK203 pKa = 10.11PKK205 pKa = 10.55SPPAWICLSPEE216 pKa = 3.57RR217 pKa = 11.84VARR220 pKa = 11.84RR221 pKa = 11.84AVLAVEE227 pKa = 4.55RR228 pKa = 11.84NRR230 pKa = 11.84GRR232 pKa = 11.84VVLDD236 pKa = 4.31PYY238 pKa = 10.4CWWAIGLKK246 pKa = 9.95RR247 pKa = 11.84RR248 pKa = 11.84LPAAFDD254 pKa = 3.43WALSLGRR261 pKa = 11.84RR262 pKa = 11.84RR263 pKa = 11.84RR264 pKa = 11.84VEE266 pKa = 3.82RR267 pKa = 11.84KK268 pKa = 9.32RR269 pKa = 11.84RR270 pKa = 11.84EE271 pKa = 4.0LAALAPDD278 pKa = 4.0PQTALRR284 pKa = 11.84MKK286 pKa = 10.23LGLGEE291 pKa = 4.2RR292 pKa = 11.84PRR294 pKa = 11.84LARR297 pKa = 11.84AAA299 pKa = 3.71

Molecular weight: 32.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3893

0

3893

1431816

30

7557

367.8

39.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.795 ± 0.054

1.051 ± 0.018

6.251 ± 0.04

6.715 ± 0.046

3.314 ± 0.023

8.764 ± 0.046

2.034 ± 0.015

3.885 ± 0.026

2.838 ± 0.032

10.041 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.03 ± 0.019

2.66 ± 0.03

5.507 ± 0.035

3.235 ± 0.024

7.04 ± 0.046

6.036 ± 0.033

5.372 ± 0.035

7.524 ± 0.031

1.493 ± 0.018

2.413 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski