Amino acid dipepetide frequency for bacterium 1xD42-67

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.85AlaAla: 11.85 ± 0.174	1.399AlaCys: 1.399 ± 0.044	5.227AlaAsp: 5.227 ± 0.079	6.353AlaGlu: 6.353 ± 0.086	3.21AlaPhe: 3.21 ± 0.072	8.259AlaGly: 8.259 ± 0.122	1.441AlaHis: 1.441 ± 0.04	4.841AlaIle: 4.841 ± 0.084	4.26AlaLys: 4.26 ± 0.072	10.855AlaLeu: 10.855 ± 0.151	2.714AlaMet: 2.714 ± 0.053	2.318AlaAsn: 2.318 ± 0.06	4.071AlaPro: 4.071 ± 0.082	3.895AlaGln: 3.895 ± 0.074	5.173AlaArg: 5.173 ± 0.082	4.479AlaSer: 4.479 ± 0.069	3.488AlaThr: 3.488 ± 0.085	7.867AlaVal: 7.867 ± 0.096	0.965AlaTrp: 0.965 ± 0.034	2.625AlaTyr: 2.625 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	1.527CysAla: 1.527 ± 0.041	0.352CysCys: 0.352 ± 0.019	0.829CysAsp: 0.829 ± 0.029	0.768CysGlu: 0.768 ± 0.028	0.598CysPhe: 0.598 ± 0.028	1.753CysGly: 1.753 ± 0.046	0.318CysHis: 0.318 ± 0.016	0.812CysIle: 0.812 ± 0.029	0.591CysLys: 0.591 ± 0.025	1.424CysLeu: 1.424 ± 0.039	0.378CysMet: 0.378 ± 0.019	0.44CysAsn: 0.44 ± 0.023	0.897CysPro: 0.897 ± 0.03	0.551CysGln: 0.551 ± 0.024	1.097CysArg: 1.097 ± 0.039	0.891CysSer: 0.891 ± 0.033	0.814CysThr: 0.814 ± 0.026	1.098CysVal: 1.098 ± 0.034	0.209CysTrp: 0.209 ± 0.014	0.521CysTyr: 0.521 ± 0.032	0.0CysXaa: 0.0 ± 0.0
Asp	4.827AspAla: 4.827 ± 0.076	0.969AspCys: 0.969 ± 0.037	2.871AspAsp: 2.871 ± 0.066	3.735AspGlu: 3.735 ± 0.069	2.351AspPhe: 2.351 ± 0.057	5.348AspGly: 5.348 ± 0.094	1.226AspHis: 1.226 ± 0.038	3.703AspIle: 3.703 ± 0.066	2.593AspLys: 2.593 ± 0.061	6.142AspLeu: 6.142 ± 0.082	1.664AspMet: 1.664 ± 0.04	1.568AspAsn: 1.568 ± 0.044	3.151AspPro: 3.151 ± 0.059	2.061AspGln: 2.061 ± 0.044	4.101AspArg: 4.101 ± 0.075	3.044AspSer: 3.044 ± 0.069	3.265AspThr: 3.265 ± 0.076	3.725AspVal: 3.725 ± 0.062	0.835AspTrp: 0.835 ± 0.029	2.303AspTyr: 2.303 ± 0.053	0.0AspXaa: 0.0 ± 0.0
Glu	6.19GluAla: 6.19 ± 0.087	0.712GluCys: 0.712 ± 0.029	4.353GluAsp: 4.353 ± 0.072	6.238GluGlu: 6.238 ± 0.109	1.994GluPhe: 1.994 ± 0.048	5.371GluGly: 5.371 ± 0.083	1.461GluHis: 1.461 ± 0.038	4.079GluIle: 4.079 ± 0.071	3.88GluLys: 3.88 ± 0.075	7.345GluLeu: 7.345 ± 0.095	1.901GluMet: 1.901 ± 0.047	2.281GluAsn: 2.281 ± 0.054	2.318GluPro: 2.318 ± 0.064	3.147GluGln: 3.147 ± 0.064	4.744GluArg: 4.744 ± 0.076	2.549GluSer: 2.549 ± 0.052	3.321GluThr: 3.321 ± 0.054	4.237GluVal: 4.237 ± 0.063	0.674GluTrp: 0.674 ± 0.029	1.991GluTyr: 1.991 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.186PheAla: 3.186 ± 0.059	0.766PheCys: 0.766 ± 0.024	2.424PheAsp: 2.424 ± 0.054	2.008PheGlu: 2.008 ± 0.047	1.618PhePhe: 1.618 ± 0.049	3.033PheGly: 3.033 ± 0.06	0.728PheHis: 0.728 ± 0.028	1.816PheIle: 1.816 ± 0.045	1.298PheLys: 1.298 ± 0.035	3.749PheLeu: 3.749 ± 0.074	0.785PheMet: 0.785 ± 0.033	1.048PheAsn: 1.048 ± 0.035	1.578PhePro: 1.578 ± 0.044	1.628PheGln: 1.628 ± 0.041	1.999PheArg: 1.999 ± 0.046	2.564PheSer: 2.564 ± 0.05	2.313PheThr: 2.313 ± 0.055	2.317PheVal: 2.317 ± 0.051	0.502PheTrp: 0.502 ± 0.024	1.353PheTyr: 1.353 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	7.11GlyAla: 7.11 ± 0.117	1.443GlyCys: 1.443 ± 0.038	4.469GlyAsp: 4.469 ± 0.07	5.303GlyGlu: 5.303 ± 0.087	2.965GlyPhe: 2.965 ± 0.057	6.992GlyGly: 6.992 ± 0.137	1.506GlyHis: 1.506 ± 0.041	4.719GlyIle: 4.719 ± 0.089	4.247GlyLys: 4.247 ± 0.069	7.656GlyLeu: 7.656 ± 0.12	2.545GlyMet: 2.545 ± 0.056	2.271GlyAsn: 2.271 ± 0.065	2.647GlyPro: 2.647 ± 0.055	2.975GlyGln: 2.975 ± 0.055	5.195GlyArg: 5.195 ± 0.079	4.661GlySer: 4.661 ± 0.079	4.845GlyThr: 4.845 ± 0.111	5.504GlyVal: 5.504 ± 0.083	1.059GlyTrp: 1.059 ± 0.04	3.121GlyTyr: 3.121 ± 0.062	0.0GlyXaa: 0.0 ± 0.0
His	1.215HisAla: 1.215 ± 0.035	0.346HisCys: 0.346 ± 0.018	0.974HisAsp: 0.974 ± 0.03	0.966HisGlu: 0.966 ± 0.032	0.79HisPhe: 0.79 ± 0.029	1.402HisGly: 1.402 ± 0.036	0.432HisHis: 0.432 ± 0.023	1.275HisIle: 1.275 ± 0.037	0.669HisLys: 0.669 ± 0.025	1.707HisLeu: 1.707 ± 0.043	0.5HisMet: 0.5 ± 0.02	0.517HisAsn: 0.517 ± 0.022	1.125HisPro: 1.125 ± 0.038	0.639HisGln: 0.639 ± 0.023	1.11HisArg: 1.11 ± 0.038	0.942HisSer: 0.942 ± 0.035	1.097HisThr: 1.097 ± 0.031	1.097HisVal: 1.097 ± 0.033	0.23HisTrp: 0.23 ± 0.015	0.708HisTyr: 0.708 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.151IleAla: 5.151 ± 0.076	0.994IleCys: 0.994 ± 0.032	3.415IleAsp: 3.415 ± 0.06	3.069IleGlu: 3.069 ± 0.06	2.049IlePhe: 2.049 ± 0.049	4.153IleGly: 4.153 ± 0.091	1.112IleHis: 1.112 ± 0.03	2.766IleIle: 2.766 ± 0.07	2.204IleLys: 2.204 ± 0.052	5.769IleLeu: 5.769 ± 0.093	1.325IleMet: 1.325 ± 0.037	1.661IleAsn: 1.661 ± 0.042	2.985IlePro: 2.985 ± 0.061	2.325IleGln: 2.325 ± 0.055	3.434IleArg: 3.434 ± 0.061	3.677IleSer: 3.677 ± 0.061	3.203IleThr: 3.203 ± 0.076	3.984IleVal: 3.984 ± 0.076	0.613IleTrp: 0.613 ± 0.029	1.786IleTyr: 1.786 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	4.351LysAla: 4.351 ± 0.076	0.484LysCys: 0.484 ± 0.028	2.891LysAsp: 2.891 ± 0.057	3.616LysGlu: 3.616 ± 0.067	1.204LysPhe: 1.204 ± 0.038	3.528LysGly: 3.528 ± 0.061	0.699LysHis: 0.699 ± 0.027	2.733LysIle: 2.733 ± 0.053	3.316LysLys: 3.316 ± 0.071	4.29LysLeu: 4.29 ± 0.072	1.28LysMet: 1.28 ± 0.038	1.712LysAsn: 1.712 ± 0.046	1.759LysPro: 1.759 ± 0.055	1.609LysGln: 1.609 ± 0.042	2.775LysArg: 2.775 ± 0.059	2.247LysSer: 2.247 ± 0.055	2.651LysThr: 2.651 ± 0.055	3.177LysVal: 3.177 ± 0.067	0.471LysTrp: 0.471 ± 0.02	1.536LysTyr: 1.536 ± 0.042	0.0LysXaa: 0.0 ± 0.0
Leu	9.874LeuAla: 9.874 ± 0.14	1.905LeuCys: 1.905 ± 0.045	6.409LeuAsp: 6.409 ± 0.082	7.201LeuGlu: 7.201 ± 0.103	3.892LeuPhe: 3.892 ± 0.08	7.176LeuGly: 7.176 ± 0.103	1.647LeuHis: 1.647 ± 0.042	4.971LeuIle: 4.971 ± 0.082	4.322LeuLys: 4.322 ± 0.068	10.31LeuLeu: 10.31 ± 0.16	2.639LeuMet: 2.639 ± 0.057	2.762LeuAsn: 2.762 ± 0.058	4.728LeuPro: 4.728 ± 0.072	2.87LeuGln: 2.87 ± 0.058	5.849LeuArg: 5.849 ± 0.105	6.927LeuSer: 6.927 ± 0.104	5.996LeuThr: 5.996 ± 0.092	6.145LeuVal: 6.145 ± 0.09	1.157LeuTrp: 1.157 ± 0.043	3.256LeuTyr: 3.256 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	2.832MetAla: 2.832 ± 0.049	0.307MetCys: 0.307 ± 0.017	2.063MetAsp: 2.063 ± 0.05	2.348MetGlu: 2.348 ± 0.057	0.813MetPhe: 0.813 ± 0.03	2.23MetGly: 2.23 ± 0.061	0.314MetHis: 0.314 ± 0.018	1.387MetIle: 1.387 ± 0.046	1.61MetLys: 1.61 ± 0.043	2.533MetLeu: 2.533 ± 0.05	0.756MetMet: 0.756 ± 0.031	0.896MetAsn: 0.896 ± 0.028	1.178MetPro: 1.178 ± 0.036	0.72MetGln: 0.72 ± 0.03	1.393MetArg: 1.393 ± 0.036	1.668MetSer: 1.668 ± 0.046	1.631MetThr: 1.631 ± 0.042	1.875MetVal: 1.875 ± 0.049	0.206MetTrp: 0.206 ± 0.013	0.662MetTyr: 0.662 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.665AsnAla: 2.665 ± 0.056	0.493AsnCys: 0.493 ± 0.025	1.65AsnAsp: 1.65 ± 0.04	1.606AsnGlu: 1.606 ± 0.046	1.022AsnPhe: 1.022 ± 0.034	2.843AsnGly: 2.843 ± 0.081	0.515AsnHis: 0.515 ± 0.025	1.984AsnIle: 1.984 ± 0.042	1.279AsnLys: 1.279 ± 0.037	2.485AsnLeu: 2.485 ± 0.046	0.805AsnMet: 0.805 ± 0.03	0.985AsnAsn: 0.985 ± 0.042	1.312AsnPro: 1.312 ± 0.033	0.948AsnGln: 0.948 ± 0.03	1.581AsnArg: 1.581 ± 0.046	1.612AsnSer: 1.612 ± 0.038	1.739AsnThr: 1.739 ± 0.051	2.054AsnVal: 2.054 ± 0.055	0.328AsnTrp: 0.328 ± 0.02	1.119AsnTyr: 1.119 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	4.668ProAla: 4.668 ± 0.089	0.627ProCys: 0.627 ± 0.027	3.097ProAsp: 3.097 ± 0.064	4.072ProGlu: 4.072 ± 0.081	1.667ProPhe: 1.667 ± 0.045	3.578ProGly: 3.578 ± 0.078	0.708ProHis: 0.708 ± 0.026	2.173ProIle: 2.173 ± 0.052	1.889ProLys: 1.889 ± 0.052	3.874ProLeu: 3.874 ± 0.067	1.155ProMet: 1.155 ± 0.035	1.223ProAsn: 1.223 ± 0.036	1.869ProPro: 1.869 ± 0.049	1.68ProGln: 1.68 ± 0.038	1.974ProArg: 1.974 ± 0.05	2.244ProSer: 2.244 ± 0.053	1.947ProThr: 1.947 ± 0.055	3.568ProVal: 3.568 ± 0.059	0.515ProTrp: 0.515 ± 0.029	1.477ProTyr: 1.477 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	3.814GlnAla: 3.814 ± 0.06	0.42GlnCys: 0.42 ± 0.022	1.956GlnAsp: 1.956 ± 0.043	2.939GlnGlu: 2.939 ± 0.08	1.254GlnPhe: 1.254 ± 0.034	2.78GlnGly: 2.78 ± 0.055	0.572GlnHis: 0.572 ± 0.024	2.274GlnIle: 2.274 ± 0.047	1.915GlnLys: 1.915 ± 0.047	3.392GlnLeu: 3.392 ± 0.063	1.104GlnMet: 1.104 ± 0.028	1.13GlnAsn: 1.13 ± 0.035	1.432GlnPro: 1.432 ± 0.042	1.382GlnGln: 1.382 ± 0.051	2.397GlnArg: 2.397 ± 0.057	1.917GlnSer: 1.917 ± 0.04	1.978GlnThr: 1.978 ± 0.048	2.786GlnVal: 2.786 ± 0.054	0.43GlnTrp: 0.43 ± 0.023	1.254GlnTyr: 1.254 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	5.368ArgAla: 5.368 ± 0.084	0.935ArgCys: 0.935 ± 0.033	3.34ArgAsp: 3.34 ± 0.059	4.632ArgGlu: 4.632 ± 0.083	2.4ArgPhe: 2.4 ± 0.05	3.88ArgGly: 3.88 ± 0.068	1.023ArgHis: 1.023 ± 0.033	3.293ArgIle: 3.293 ± 0.053	2.83ArgLys: 2.83 ± 0.067	5.901ArgLeu: 5.901 ± 0.1	1.851ArgMet: 1.851 ± 0.045	1.616ArgAsn: 1.616 ± 0.041	2.752ArgPro: 2.752 ± 0.058	2.531ArgGln: 2.531 ± 0.062	4.575ArgArg: 4.575 ± 0.088	3.293ArgSer: 3.293 ± 0.061	3.087ArgThr: 3.087 ± 0.056	3.338ArgVal: 3.338 ± 0.059	0.812ArgTrp: 0.812 ± 0.033	2.467ArgTyr: 2.467 ± 0.057	0.0ArgXaa: 0.0 ± 0.0
Ser	5.353SerAla: 5.353 ± 0.081	0.9SerCys: 0.9 ± 0.03	3.013SerAsp: 3.013 ± 0.064	3.138SerGlu: 3.138 ± 0.066	2.344SerPhe: 2.344 ± 0.044	5.568SerGly: 5.568 ± 0.083	1.044SerHis: 1.044 ± 0.033	3.176SerIle: 3.176 ± 0.063	2.351SerLys: 2.351 ± 0.049	5.444SerLeu: 5.444 ± 0.078	1.53SerMet: 1.53 ± 0.041	1.599SerAsn: 1.599 ± 0.049	2.574SerPro: 2.574 ± 0.055	2.126SerGln: 2.126 ± 0.05	3.258SerArg: 3.258 ± 0.063	3.387SerSer: 3.387 ± 0.08	2.715SerThr: 2.715 ± 0.055	3.904SerVal: 3.904 ± 0.077	0.654SerTrp: 0.654 ± 0.023	2.045SerTyr: 2.045 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	5.933ThrAla: 5.933 ± 0.102	0.698ThrCys: 0.698 ± 0.025	3.165ThrAsp: 3.165 ± 0.073	3.097ThrGlu: 3.097 ± 0.059	2.105ThrPhe: 2.105 ± 0.043	5.072ThrGly: 5.072 ± 0.1	0.911ThrHis: 0.911 ± 0.029	3.351ThrIle: 3.351 ± 0.079	2.032ThrLys: 2.032 ± 0.041	5.051ThrLeu: 5.051 ± 0.087	1.414ThrMet: 1.414 ± 0.036	1.535ThrAsn: 1.535 ± 0.05	2.514ThrPro: 2.514 ± 0.06	1.725ThrGln: 1.725 ± 0.044	2.398ThrArg: 2.398 ± 0.049	2.769ThrSer: 2.769 ± 0.06	2.779ThrThr: 2.779 ± 0.073	4.996ThrVal: 4.996 ± 0.124	0.662ThrTrp: 0.662 ± 0.032	1.832ThrTyr: 1.832 ± 0.061	0.0ThrXaa: 0.0 ± 0.0
Val	5.549ValAla: 5.549 ± 0.092	1.294ValCys: 1.294 ± 0.04	4.202ValAsp: 4.202 ± 0.079	4.709ValGlu: 4.709 ± 0.076	2.618ValPhe: 2.618 ± 0.049	4.749ValGly: 4.749 ± 0.078	1.109ValHis: 1.109 ± 0.031	3.803ValIle: 3.803 ± 0.066	3.181ValLys: 3.181 ± 0.064	7.503ValLeu: 7.503 ± 0.105	1.939ValMet: 1.939 ± 0.047	1.901ValAsn: 1.901 ± 0.045	3.307ValPro: 3.307 ± 0.06	2.374ValGln: 2.374 ± 0.053	4.032ValArg: 4.032 ± 0.067	4.646ValSer: 4.646 ± 0.071	4.383ValThr: 4.383 ± 0.129	4.919ValVal: 4.919 ± 0.092	0.826ValTrp: 0.826 ± 0.026	2.299ValTyr: 2.299 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	1.014TrpAla: 1.014 ± 0.033	0.227TrpCys: 0.227 ± 0.017	0.73TrpAsp: 0.73 ± 0.028	0.829TrpGlu: 0.829 ± 0.034	0.421TrpPhe: 0.421 ± 0.026	0.866TrpGly: 0.866 ± 0.031	0.22TrpHis: 0.22 ± 0.015	0.523TrpIle: 0.523 ± 0.022	0.601TrpLys: 0.601 ± 0.028	1.231TrpLeu: 1.231 ± 0.04	0.347TrpMet: 0.347 ± 0.019	0.44TrpAsn: 0.44 ± 0.02	0.425TrpPro: 0.425 ± 0.021	0.448TrpGln: 0.448 ± 0.025	0.734TrpArg: 0.734 ± 0.031	0.715TrpSer: 0.715 ± 0.027	0.658TrpThr: 0.658 ± 0.027	0.656TrpVal: 0.656 ± 0.027	0.15TrpTrp: 0.15 ± 0.013	0.451TrpTyr: 0.451 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.815TyrAla: 2.815 ± 0.055	0.598TyrCys: 0.598 ± 0.024	2.403TyrAsp: 2.403 ± 0.07	2.157TyrGlu: 2.157 ± 0.044	1.398TyrPhe: 1.398 ± 0.036	2.652TyrGly: 2.652 ± 0.048	0.754TyrHis: 0.754 ± 0.023	1.998TyrIle: 1.998 ± 0.046	1.259TyrLys: 1.259 ± 0.04	3.374TyrLeu: 3.374 ± 0.064	0.788TyrMet: 0.788 ± 0.027	1.123TyrAsn: 1.123 ± 0.038	1.387TyrPro: 1.387 ± 0.037	1.49TyrGln: 1.49 ± 0.041	2.106TyrArg: 2.106 ± 0.051	1.891TyrSer: 1.891 ± 0.048	2.129TyrThr: 2.129 ± 0.06	2.096TyrVal: 2.096 ± 0.049	0.389TyrTrp: 0.389 ± 0.021	1.346TyrTyr: 1.346 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3363 proteins (1021562 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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