Diaporthe helianthi

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Diaporthales; Diaporthaceae; Diaporthe

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P5ICH9|A0A2P5ICH9_9PEZI TLP1_add_C domain-containing protein OS=Diaporthe helianthi OX=158607 GN=DHEL01_v201359 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.34ACAEE6 pKa = 4.08PALLALLLAVTASAKK21 pKa = 9.22VVRR24 pKa = 11.84IPVARR29 pKa = 11.84NSPGATVSRR38 pKa = 11.84RR39 pKa = 11.84HH40 pKa = 5.97HH41 pKa = 6.43PRR43 pKa = 11.84LTPRR47 pKa = 11.84ASFTEE52 pKa = 4.07SLVNNITGGGYY63 pKa = 8.88YY64 pKa = 10.59ASVSVGTPGQDD75 pKa = 2.42IDD77 pKa = 4.59MILDD81 pKa = 3.81TGSSDD86 pKa = 3.54AFIVAAGADD95 pKa = 3.92LCQSPRR101 pKa = 11.84LQLIYY106 pKa = 9.38QTQCGQTFDD115 pKa = 4.26PSRR118 pKa = 11.84SSTFDD123 pKa = 3.07VVVPDD128 pKa = 4.37GFQIQYY134 pKa = 10.66LDD136 pKa = 3.53GSTASGDD143 pKa = 3.87YY144 pKa = 9.63ITDD147 pKa = 3.28HH148 pKa = 6.83FEE150 pKa = 4.58IGDD153 pKa = 3.92TVIEE157 pKa = 4.14SLQMGLANQTASGTGVLGIGYY178 pKa = 6.69TANEE182 pKa = 4.23AAEE185 pKa = 4.17TLYY188 pKa = 10.66PNLVDD193 pKa = 4.89QMVAQDD199 pKa = 4.83FIPTKK204 pKa = 10.5AYY206 pKa = 10.58SLYY209 pKa = 10.95LNDD212 pKa = 4.1YY213 pKa = 9.53QSSTGSILFGGVDD226 pKa = 3.45TEE228 pKa = 4.26KK229 pKa = 10.88FIGGLTVIPVLRR241 pKa = 11.84DD242 pKa = 3.15AQTQDD247 pKa = 3.19FTSFTVGMTAISYY260 pKa = 8.17AFPNGTTGDD269 pKa = 3.53ASLQGGSLDD278 pKa = 4.87AILDD282 pKa = 3.76SGTTLSYY289 pKa = 10.87FPDD292 pKa = 3.83SVATPLFEE300 pKa = 5.31ALGAFTYY307 pKa = 10.72SSLGSVGLTMVEE319 pKa = 4.27CSLATSGLEE328 pKa = 3.56LTFRR332 pKa = 11.84FNDD335 pKa = 3.47SVSIVVPADD344 pKa = 3.28EE345 pKa = 4.33VVIDD349 pKa = 3.92AFGPIEE355 pKa = 4.29GNIPPEE361 pKa = 4.39IPLEE365 pKa = 4.13QPCLFGIQNSGDD377 pKa = 3.8SLPSEE382 pKa = 4.75GARR385 pKa = 11.84QIDD388 pKa = 3.84FALLGDD394 pKa = 3.95TFLRR398 pKa = 11.84SAYY401 pKa = 9.94VVYY404 pKa = 10.81DD405 pKa = 3.9LDD407 pKa = 3.98NNEE410 pKa = 3.9IAMAQANLNSTDD422 pKa = 3.51TNVQEE427 pKa = 4.31LTEE430 pKa = 4.41SSDD433 pKa = 3.31LSSFEE438 pKa = 4.76GVEE441 pKa = 4.01QQQQSSGTSGGGSSGTDD458 pKa = 3.11SSSSAGSSGTLSQDD472 pKa = 3.39FGRR475 pKa = 11.84CEE477 pKa = 3.76FGLEE481 pKa = 3.87CGYY484 pKa = 9.99GQRR487 pKa = 11.84GRR489 pKa = 11.84GGDD492 pKa = 3.76CDD494 pKa = 5.19DD495 pKa = 6.16DD496 pKa = 3.66EE497 pKa = 4.78HH498 pKa = 8.29QRR500 pKa = 11.84DD501 pKa = 3.63LLFDD505 pKa = 4.54CGLGAVRR512 pKa = 11.84PPFTFGQCQQDD523 pKa = 3.41RR524 pKa = 11.84AQQQ527 pKa = 3.21

Molecular weight: 55.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P5HX61|A0A2P5HX61_9PEZI Uncharacterized protein OS=Diaporthe helianthi OX=158607 GN=DHEL01_v206758 PE=3 SV=1
MM1 pKa = 7.82DD2 pKa = 5.28SDD4 pKa = 4.27MTTMHH9 pKa = 6.94HH10 pKa = 6.22SLSGSSQCPATLASPQLLARR30 pKa = 11.84SVDD33 pKa = 3.76AILRR37 pKa = 11.84PDD39 pKa = 3.39HH40 pKa = 6.58VFSLSRR46 pKa = 11.84LVSISLPGIYY56 pKa = 9.21TRR58 pKa = 11.84PHH60 pKa = 5.27TKK62 pKa = 9.96GVNGVGLGPWYY73 pKa = 10.32RR74 pKa = 11.84AVLGLLPPNYY84 pKa = 9.87EE85 pKa = 3.95NLLCRR90 pKa = 11.84RR91 pKa = 11.84LGVQSIWASPIDD103 pKa = 4.27LLQPPAASVRR113 pKa = 11.84VCSKK117 pKa = 10.15ISTSGSFPAADD128 pKa = 3.9FRR130 pKa = 11.84IALIGRR136 pKa = 11.84MPQQLFVHH144 pKa = 6.67LSSAPRR150 pKa = 11.84CHH152 pKa = 6.92DD153 pKa = 3.23VALRR157 pKa = 11.84RR158 pKa = 11.84SPKK161 pKa = 10.04LIMRR165 pKa = 11.84STHH168 pKa = 6.28PPGSFCAPNLQARR181 pKa = 11.84SMWASWAAGHH191 pKa = 5.95QRR193 pKa = 11.84VNHH196 pKa = 6.1GLPLATWGLGQRR208 pKa = 11.84LKK210 pKa = 10.01TLPGDD215 pKa = 3.11SRR217 pKa = 11.84YY218 pKa = 9.54RR219 pKa = 11.84VSRR222 pKa = 11.84AGFTGPHH229 pKa = 5.98KK230 pKa = 10.72CWLTGMAKK238 pKa = 10.12KK239 pKa = 9.91RR240 pKa = 11.84DD241 pKa = 3.88KK242 pKa = 9.93PTEE245 pKa = 3.68LAEE248 pKa = 4.05QGRR251 pKa = 11.84LQIPRR256 pKa = 11.84TLSDD260 pKa = 3.54LGGSSSQNPALIGRR274 pKa = 11.84TPAVWCTIRR283 pKa = 11.84GIAMLIATDD292 pKa = 3.97SLLWWLEE299 pKa = 3.88CALVMAIAPQLGSTAPVVPQII320 pKa = 4.18

Molecular weight: 34.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13137

0

13137

6232127

26

5754

474.4

52.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.218 ± 0.019

1.195 ± 0.008

5.886 ± 0.016

5.931 ± 0.02

3.628 ± 0.013

7.458 ± 0.02

2.379 ± 0.01

4.471 ± 0.015

4.581 ± 0.021

8.643 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.188 ± 0.007

3.553 ± 0.011

6.233 ± 0.023

4.028 ± 0.015

6.147 ± 0.019

8.102 ± 0.026

5.927 ± 0.015

6.271 ± 0.016

1.508 ± 0.008

2.649 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski