Amino acid dipepetide frequency for Bacillus phage W.Ph.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.888AlaAla: 3.888 ± 0.654
0.291AlaCys: 0.291 ± 0.077
3.306AlaAsp: 3.306 ± 0.304
4.179AlaGlu: 4.179 ± 0.355
2.432AlaPhe: 2.432 ± 0.233
3.306AlaGly: 3.306 ± 0.386
1.102AlaHis: 1.102 ± 0.161
3.971AlaIle: 3.971 ± 0.302
4.615AlaLys: 4.615 ± 0.298
4.345AlaLeu: 4.345 ± 0.29
1.746AlaMet: 1.746 ± 0.254
2.952AlaAsn: 2.952 ± 0.28
1.518AlaPro: 1.518 ± 0.242
1.933AlaGln: 1.933 ± 0.229
2.308AlaArg: 2.308 ± 0.208
2.911AlaSer: 2.911 ± 0.334
3.701AlaThr: 3.701 ± 0.456
3.326AlaVal: 3.326 ± 0.252
0.603AlaTrp: 0.603 ± 0.102
2.474AlaTyr: 2.474 ± 0.219
0.0AlaXaa: 0.0 ± 0.0
Cys
0.312CysAla: 0.312 ± 0.089
0.187CysCys: 0.187 ± 0.077
0.79CysAsp: 0.79 ± 0.146
0.728CysGlu: 0.728 ± 0.151
0.291CysPhe: 0.291 ± 0.076
0.686CysGly: 0.686 ± 0.135
0.249CysHis: 0.249 ± 0.076
0.561CysIle: 0.561 ± 0.108
0.79CysLys: 0.79 ± 0.142
0.374CysLeu: 0.374 ± 0.085
0.374CysMet: 0.374 ± 0.098
0.478CysAsn: 0.478 ± 0.107
0.395CysPro: 0.395 ± 0.1
0.229CysGln: 0.229 ± 0.071
0.312CysArg: 0.312 ± 0.09
0.437CysSer: 0.437 ± 0.096
0.561CysThr: 0.561 ± 0.111
0.707CysVal: 0.707 ± 0.113
0.187CysTrp: 0.187 ± 0.061
0.374CysTyr: 0.374 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
3.68AspAla: 3.68 ± 0.302
0.644AspCys: 0.644 ± 0.13
4.033AspAsp: 4.033 ± 0.335
5.717AspGlu: 5.717 ± 0.425
2.848AspPhe: 2.848 ± 0.228
4.137AspGly: 4.137 ± 0.347
0.748AspHis: 0.748 ± 0.132
5.468AspIle: 5.468 ± 0.334
6.029AspLys: 6.029 ± 0.368
5.26AspLeu: 5.26 ± 0.355
2.121AspMet: 2.121 ± 0.235
3.534AspAsn: 3.534 ± 0.243
1.58AspPro: 1.58 ± 0.213
0.811AspGln: 0.811 ± 0.132
2.931AspArg: 2.931 ± 0.219
3.805AspSer: 3.805 ± 0.258
3.119AspThr: 3.119 ± 0.253
4.99AspVal: 4.99 ± 0.333
0.977AspTrp: 0.977 ± 0.149
3.16AspTyr: 3.16 ± 0.259
0.0AspXaa: 0.0 ± 0.0
Glu
3.805GluAla: 3.805 ± 0.315
0.894GluCys: 0.894 ± 0.144
4.657GluAsp: 4.657 ± 0.335
8.711GluGlu: 8.711 ± 0.904
3.597GluPhe: 3.597 ± 0.31
4.844GluGly: 4.844 ± 0.325
1.58GluHis: 1.58 ± 0.19
5.135GluIle: 5.135 ± 0.319
6.008GluLys: 6.008 ± 0.458
8.378GluLeu: 8.378 ± 0.514
3.181GluMet: 3.181 ± 0.245
4.262GluAsn: 4.262 ± 0.377
1.933GluPro: 1.933 ± 0.188
2.807GluGln: 2.807 ± 0.239
3.638GluArg: 3.638 ± 0.298
4.511GluSer: 4.511 ± 0.272
3.971GluThr: 3.971 ± 0.238
6.466GluVal: 6.466 ± 0.58
0.998GluTrp: 0.998 ± 0.157
3.326GluTyr: 3.326 ± 0.264
0.0GluXaa: 0.0 ± 0.0
Phe
2.1PheAla: 2.1 ± 0.238
0.437PheCys: 0.437 ± 0.096
3.015PheAsp: 3.015 ± 0.224
2.786PheGlu: 2.786 ± 0.244
1.372PhePhe: 1.372 ± 0.174
1.954PheGly: 1.954 ± 0.195
0.811PheHis: 0.811 ± 0.117
2.64PheIle: 2.64 ± 0.227
3.326PheLys: 3.326 ± 0.31
2.89PheLeu: 2.89 ± 0.24
1.31PheMet: 1.31 ± 0.149
2.703PheAsn: 2.703 ± 0.23
1.06PhePro: 1.06 ± 0.151
0.894PheGln: 0.894 ± 0.134
1.538PheArg: 1.538 ± 0.171
2.391PheSer: 2.391 ± 0.206
3.222PheThr: 3.222 ± 0.254
2.765PheVal: 2.765 ± 0.24
0.395PheTrp: 0.395 ± 0.104
1.684PheTyr: 1.684 ± 0.177
0.0PheXaa: 0.0 ± 0.0
Gly
3.056GlyAla: 3.056 ± 0.589
0.561GlyCys: 0.561 ± 0.093
3.285GlyAsp: 3.285 ± 0.276
4.428GlyGlu: 4.428 ± 0.25
2.516GlyPhe: 2.516 ± 0.196
4.844GlyGly: 4.844 ± 0.949
1.04GlyHis: 1.04 ± 0.152
4.054GlyIle: 4.054 ± 0.376
4.802GlyLys: 4.802 ± 0.36
4.22GlyLeu: 4.22 ± 0.379
1.58GlyMet: 1.58 ± 0.163
3.035GlyAsn: 3.035 ± 0.29
0.541GlyPro: 0.541 ± 0.089
1.622GlyGln: 1.622 ± 0.225
2.62GlyArg: 2.62 ± 0.204
3.742GlySer: 3.742 ± 0.355
4.096GlyThr: 4.096 ± 0.389
5.135GlyVal: 5.135 ± 0.294
1.143GlyTrp: 1.143 ± 0.169
3.368GlyTyr: 3.368 ± 0.28
0.0GlyXaa: 0.0 ± 0.0
His
0.873HisAla: 0.873 ± 0.129
0.229HisCys: 0.229 ± 0.061
1.06HisAsp: 1.06 ± 0.154
1.435HisGlu: 1.435 ± 0.174
0.665HisPhe: 0.665 ± 0.126
1.081HisGly: 1.081 ± 0.161
0.416HisHis: 0.416 ± 0.103
1.351HisIle: 1.351 ± 0.168
1.455HisLys: 1.455 ± 0.189
1.393HisLeu: 1.393 ± 0.172
0.561HisMet: 0.561 ± 0.1
1.081HisAsn: 1.081 ± 0.164
0.561HisPro: 0.561 ± 0.118
0.561HisGln: 0.561 ± 0.111
0.769HisArg: 0.769 ± 0.117
1.247HisSer: 1.247 ± 0.162
1.102HisThr: 1.102 ± 0.164
1.538HisVal: 1.538 ± 0.185
0.249HisTrp: 0.249 ± 0.062
1.04HisTyr: 1.04 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
3.992IleAla: 3.992 ± 0.292
0.707IleCys: 0.707 ± 0.113
5.426IleAsp: 5.426 ± 0.315
5.551IleGlu: 5.551 ± 0.36
1.622IlePhe: 1.622 ± 0.158
3.742IleGly: 3.742 ± 0.346
1.247IleHis: 1.247 ± 0.195
4.699IleIle: 4.699 ± 0.311
6.466IleLys: 6.466 ± 0.393
4.304IleLeu: 4.304 ± 0.279
1.933IleMet: 1.933 ± 0.186
3.971IleAsn: 3.971 ± 0.305
2.121IlePro: 2.121 ± 0.233
2.121IleGln: 2.121 ± 0.19
3.16IleArg: 3.16 ± 0.252
4.47IleSer: 4.47 ± 0.279
4.615IleThr: 4.615 ± 0.415
4.678IleVal: 4.678 ± 0.353
0.478IleTrp: 0.478 ± 0.099
2.37IleTyr: 2.37 ± 0.209
0.0IleXaa: 0.0 ± 0.0
Lys
4.74LysAla: 4.74 ± 0.408
0.582LysCys: 0.582 ± 0.123
5.509LysAsp: 5.509 ± 0.312
8.482LysGlu: 8.482 ± 0.565
2.62LysPhe: 2.62 ± 0.223
5.489LysGly: 5.489 ± 0.367
1.913LysHis: 1.913 ± 0.209
4.636LysIle: 4.636 ± 0.319
6.112LysLys: 6.112 ± 0.458
6.778LysLeu: 6.778 ± 0.397
2.682LysMet: 2.682 ± 0.269
4.116LysAsn: 4.116 ± 0.296
2.578LysPro: 2.578 ± 0.234
3.243LysGln: 3.243 ± 0.26
3.659LysArg: 3.659 ± 0.279
4.595LysSer: 4.595 ± 0.366
4.428LysThr: 4.428 ± 0.307
5.447LysVal: 5.447 ± 0.334
1.06LysTrp: 1.06 ± 0.134
3.555LysTyr: 3.555 ± 0.291
0.0LysXaa: 0.0 ± 0.0
Leu
4.283LeuAla: 4.283 ± 0.284
0.748LeuCys: 0.748 ± 0.141
5.593LeuAsp: 5.593 ± 0.34
7.027LeuGlu: 7.027 ± 0.533
2.682LeuPhe: 2.682 ± 0.249
4.366LeuGly: 4.366 ± 0.336
1.185LeuHis: 1.185 ± 0.181
4.782LeuIle: 4.782 ± 0.321
6.029LeuLys: 6.029 ± 0.348
5.696LeuLeu: 5.696 ± 0.385
2.037LeuMet: 2.037 ± 0.205
4.137LeuAsn: 4.137 ± 0.292
2.516LeuPro: 2.516 ± 0.219
2.931LeuGln: 2.931 ± 0.228
3.451LeuArg: 3.451 ± 0.268
5.53LeuSer: 5.53 ± 0.299
5.489LeuThr: 5.489 ± 0.349
5.301LeuVal: 5.301 ± 0.34
0.832LeuTrp: 0.832 ± 0.136
3.285LeuTyr: 3.285 ± 0.29
0.0LeuXaa: 0.0 ± 0.0
Met
1.58MetAla: 1.58 ± 0.206
0.187MetCys: 0.187 ± 0.06
1.705MetAsp: 1.705 ± 0.193
2.121MetGlu: 2.121 ± 0.223
1.247MetPhe: 1.247 ± 0.145
1.601MetGly: 1.601 ± 0.212
0.541MetHis: 0.541 ± 0.1
1.746MetIle: 1.746 ± 0.188
3.119MetLys: 3.119 ± 0.276
2.869MetLeu: 2.869 ± 0.233
0.936MetMet: 0.936 ± 0.165
1.975MetAsn: 1.975 ± 0.188
0.686MetPro: 0.686 ± 0.115
0.686MetGln: 0.686 ± 0.114
1.414MetArg: 1.414 ± 0.151
2.64MetSer: 2.64 ± 0.214
2.536MetThr: 2.536 ± 0.265
1.726MetVal: 1.726 ± 0.173
0.249MetTrp: 0.249 ± 0.07
1.58MetTyr: 1.58 ± 0.177
0.0MetXaa: 0.0 ± 0.0
Asn
2.931AsnAla: 2.931 ± 0.237
0.374AsnCys: 0.374 ± 0.113
3.451AsnAsp: 3.451 ± 0.239
4.137AsnGlu: 4.137 ± 0.321
2.474AsnPhe: 2.474 ± 0.231
3.992AsnGly: 3.992 ± 0.302
0.873AsnHis: 0.873 ± 0.133
3.909AsnIle: 3.909 ± 0.264
4.865AsnLys: 4.865 ± 0.316
3.721AsnLeu: 3.721 ± 0.277
2.141AsnMet: 2.141 ± 0.206
3.888AsnAsn: 3.888 ± 0.322
2.328AsnPro: 2.328 ± 0.293
1.746AsnGln: 1.746 ± 0.19
2.723AsnArg: 2.723 ± 0.263
2.911AsnSer: 2.911 ± 0.246
3.805AsnThr: 3.805 ± 0.298
3.763AsnVal: 3.763 ± 0.301
0.644AsnTrp: 0.644 ± 0.115
2.682AsnTyr: 2.682 ± 0.257
0.0AsnXaa: 0.0 ± 0.0
Pro
1.538ProAla: 1.538 ± 0.19
0.249ProCys: 0.249 ± 0.076
2.578ProAsp: 2.578 ± 0.334
2.37ProGlu: 2.37 ± 0.253
1.435ProPhe: 1.435 ± 0.195
0.0ProGly: 0.0 ± 0.0
0.541ProHis: 0.541 ± 0.125
1.746ProIle: 1.746 ± 0.181
2.37ProLys: 2.37 ± 0.274
1.684ProLeu: 1.684 ± 0.205
0.665ProMet: 0.665 ± 0.119
1.975ProAsn: 1.975 ± 0.286
0.707ProPro: 0.707 ± 0.12
0.936ProGln: 0.936 ± 0.131
1.164ProArg: 1.164 ± 0.147
1.83ProSer: 1.83 ± 0.204
2.37ProThr: 2.37 ± 0.209
1.954ProVal: 1.954 ± 0.215
0.229ProTrp: 0.229 ± 0.07
1.268ProTyr: 1.268 ± 0.165
0.0ProXaa: 0.0 ± 0.0
Gln
2.121GlnAla: 2.121 ± 0.268
0.229GlnCys: 0.229 ± 0.061
1.913GlnAsp: 1.913 ± 0.221
2.308GlnGlu: 2.308 ± 0.215
1.227GlnPhe: 1.227 ± 0.172
1.767GlnGly: 1.767 ± 0.181
0.915GlnHis: 0.915 ± 0.156
1.83GlnIle: 1.83 ± 0.194
1.933GlnLys: 1.933 ± 0.236
2.703GlnLeu: 2.703 ± 0.242
1.04GlnMet: 1.04 ± 0.182
1.476GlnAsn: 1.476 ± 0.183
0.811GlnPro: 0.811 ± 0.145
1.393GlnGln: 1.393 ± 0.191
1.538GlnArg: 1.538 ± 0.176
1.58GlnSer: 1.58 ± 0.191
1.892GlnThr: 1.892 ± 0.208
2.412GlnVal: 2.412 ± 0.235
0.312GlnTrp: 0.312 ± 0.078
1.164GlnTyr: 1.164 ± 0.144
0.0GlnXaa: 0.0 ± 0.0
Arg
2.682ArgAla: 2.682 ± 0.252
0.416ArgCys: 0.416 ± 0.096
2.807ArgAsp: 2.807 ± 0.3
4.054ArgGlu: 4.054 ± 0.336
1.705ArgPhe: 1.705 ± 0.212
2.786ArgGly: 2.786 ± 0.234
0.852ArgHis: 0.852 ± 0.142
3.015ArgIle: 3.015 ± 0.284
3.181ArgLys: 3.181 ± 0.275
3.68ArgLeu: 3.68 ± 0.312
1.663ArgMet: 1.663 ± 0.222
2.765ArgAsn: 2.765 ± 0.213
1.206ArgPro: 1.206 ± 0.155
1.351ArgGln: 1.351 ± 0.191
1.892ArgArg: 1.892 ± 0.226
1.933ArgSer: 1.933 ± 0.264
2.432ArgThr: 2.432 ± 0.25
3.68ArgVal: 3.68 ± 0.262
0.437ArgTrp: 0.437 ± 0.105
1.933ArgTyr: 1.933 ± 0.189
0.0ArgXaa: 0.0 ± 0.0
Ser
3.451SerAla: 3.451 ± 0.325
0.499SerCys: 0.499 ± 0.106
3.805SerAsp: 3.805 ± 0.307
3.617SerGlu: 3.617 ± 0.288
2.723SerPhe: 2.723 ± 0.244
3.659SerGly: 3.659 ± 0.38
1.04SerHis: 1.04 ± 0.149
4.532SerIle: 4.532 ± 0.283
5.052SerLys: 5.052 ± 0.332
4.99SerLeu: 4.99 ± 0.298
1.996SerMet: 1.996 ± 0.186
3.597SerAsn: 3.597 ± 0.294
1.767SerPro: 1.767 ± 0.228
1.642SerGln: 1.642 ± 0.239
2.931SerArg: 2.931 ± 0.222
3.472SerSer: 3.472 ± 0.305
3.597SerThr: 3.597 ± 0.263
3.617SerVal: 3.617 ± 0.276
0.769SerTrp: 0.769 ± 0.108
2.931SerTyr: 2.931 ± 0.258
0.0SerXaa: 0.0 ± 0.0
Thr
3.555ThrAla: 3.555 ± 0.424
0.561ThrCys: 0.561 ± 0.097
4.096ThrAsp: 4.096 ± 0.293
4.719ThrGlu: 4.719 ± 0.319
3.056ThrPhe: 3.056 ± 0.294
4.387ThrGly: 4.387 ± 0.412
1.123ThrHis: 1.123 ± 0.146
4.74ThrIle: 4.74 ± 0.321
4.782ThrLys: 4.782 ± 0.303
5.405ThrLeu: 5.405 ± 0.374
1.663ThrMet: 1.663 ± 0.174
3.451ThrAsn: 3.451 ± 0.257
2.121ThrPro: 2.121 ± 0.268
1.518ThrGln: 1.518 ± 0.193
3.056ThrArg: 3.056 ± 0.265
3.617ThrSer: 3.617 ± 0.285
3.41ThrThr: 3.41 ± 0.29
4.761ThrVal: 4.761 ± 0.317
0.457ThrTrp: 0.457 ± 0.092
2.827ThrTyr: 2.827 ± 0.26
0.0ThrXaa: 0.0 ± 0.0
Val
3.597ValAla: 3.597 ± 0.268
0.624ValCys: 0.624 ± 0.134
4.802ValAsp: 4.802 ± 0.325
5.551ValGlu: 5.551 ± 0.402
2.64ValPhe: 2.64 ± 0.262
3.867ValGly: 3.867 ± 0.248
1.289ValHis: 1.289 ± 0.17
4.657ValIle: 4.657 ± 0.336
6.424ValLys: 6.424 ± 0.375
4.99ValLeu: 4.99 ± 0.34
1.83ValMet: 1.83 ± 0.206
4.366ValAsn: 4.366 ± 0.325
1.996ValPro: 1.996 ± 0.231
2.37ValGln: 2.37 ± 0.195
3.015ValArg: 3.015 ± 0.296
4.387ValSer: 4.387 ± 0.323
5.239ValThr: 5.239 ± 0.37
5.343ValVal: 5.343 ± 0.413
0.769ValTrp: 0.769 ± 0.147
3.555ValTyr: 3.555 ± 0.279
0.0ValXaa: 0.0 ± 0.0
Trp
0.686TrpAla: 0.686 ± 0.103
0.146TrpCys: 0.146 ± 0.05
1.019TrpAsp: 1.019 ± 0.136
0.998TrpGlu: 0.998 ± 0.129
0.395TrpPhe: 0.395 ± 0.091
0.748TrpGly: 0.748 ± 0.119
0.312TrpHis: 0.312 ± 0.075
0.769TrpIle: 0.769 ± 0.12
0.852TrpLys: 0.852 ± 0.133
0.852TrpLeu: 0.852 ± 0.146
0.249TrpMet: 0.249 ± 0.064
0.644TrpAsn: 0.644 ± 0.125
0.0TrpPro: 0.0 ± 0.0
0.395TrpGln: 0.395 ± 0.097
0.312TrpArg: 0.312 ± 0.088
0.624TrpSer: 0.624 ± 0.115
0.811TrpThr: 0.811 ± 0.179
0.894TrpVal: 0.894 ± 0.138
0.208TrpTrp: 0.208 ± 0.061
0.644TrpTyr: 0.644 ± 0.101
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.037TyrAla: 2.037 ± 0.211
0.457TyrCys: 0.457 ± 0.105
2.744TyrAsp: 2.744 ± 0.245
3.784TyrGlu: 3.784 ± 0.349
1.746TyrPhe: 1.746 ± 0.175
2.432TyrGly: 2.432 ± 0.21
0.894TyrHis: 0.894 ± 0.147
3.43TyrIle: 3.43 ± 0.243
4.033TyrLys: 4.033 ± 0.315
3.368TyrLeu: 3.368 ± 0.25
1.414TyrMet: 1.414 ± 0.159
2.952TyrAsn: 2.952 ± 0.225
1.185TyrPro: 1.185 ± 0.133
1.372TyrGln: 1.372 ± 0.161
2.037TyrArg: 2.037 ± 0.201
3.056TyrSer: 3.056 ± 0.229
2.911TyrThr: 2.911 ± 0.243
2.807TyrVal: 2.807 ± 0.186
0.561TyrTrp: 0.561 ± 0.102
2.1TyrTyr: 2.1 ± 0.242
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 274 proteins (48101 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski