Amino acid dipepetide frequency for Chloroflexi bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.503AlaAla: 9.503 ± 0.245
0.558AlaCys: 0.558 ± 0.051
5.483AlaAsp: 5.483 ± 0.12
4.71AlaGlu: 4.71 ± 0.154
3.256AlaPhe: 3.256 ± 0.112
6.771AlaGly: 6.771 ± 0.17
1.637AlaHis: 1.637 ± 0.078
6.884AlaIle: 6.884 ± 0.162
5.241AlaLys: 5.241 ± 0.161
7.948AlaLeu: 7.948 ± 0.176
2.199AlaMet: 2.199 ± 0.098
3.505AlaAsn: 3.505 ± 0.101
3.325AlaPro: 3.325 ± 0.125
3.54AlaGln: 3.54 ± 0.096
4.089AlaArg: 4.089 ± 0.125
5.909AlaSer: 5.909 ± 0.147
6.215AlaThr: 6.215 ± 0.147
6.793AlaVal: 6.793 ± 0.149
0.931AlaTrp: 0.931 ± 0.052
2.732AlaTyr: 2.732 ± 0.096
0.0AlaXaa: 0.0 ± 0.0
Cys
0.612CysAla: 0.612 ± 0.064
0.06CysCys: 0.06 ± 0.014
0.366CysAsp: 0.366 ± 0.04
0.325CysGlu: 0.325 ± 0.035
0.24CysPhe: 0.24 ± 0.029
0.527CysGly: 0.527 ± 0.04
0.133CysHis: 0.133 ± 0.02
0.363CysIle: 0.363 ± 0.034
0.233CysLys: 0.233 ± 0.031
0.577CysLeu: 0.577 ± 0.043
0.164CysMet: 0.164 ± 0.021
0.224CysAsn: 0.224 ± 0.026
0.252CysPro: 0.252 ± 0.029
0.252CysGln: 0.252 ± 0.033
0.306CysArg: 0.306 ± 0.035
0.401CysSer: 0.401 ± 0.034
0.416CysThr: 0.416 ± 0.039
0.413CysVal: 0.413 ± 0.033
0.221CysTrp: 0.221 ± 0.062
0.265CysTyr: 0.265 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
5.465AspAla: 5.465 ± 0.137
0.407AspCys: 0.407 ± 0.037
3.859AspAsp: 3.859 ± 0.133
3.862AspGlu: 3.862 ± 0.115
2.395AspPhe: 2.395 ± 0.082
4.676AspGly: 4.676 ± 0.133
1.095AspHis: 1.095 ± 0.06
4.307AspIle: 4.307 ± 0.117
3.265AspLys: 3.265 ± 0.094
4.559AspLeu: 4.559 ± 0.123
1.398AspMet: 1.398 ± 0.063
2.395AspAsn: 2.395 ± 0.091
2.18AspPro: 2.18 ± 0.08
2.534AspGln: 2.534 ± 0.095
3.076AspArg: 3.076 ± 0.105
3.695AspSer: 3.695 ± 0.11
3.915AspThr: 3.915 ± 0.114
4.303AspVal: 4.303 ± 0.112
0.757AspTrp: 0.757 ± 0.047
2.253AspTyr: 2.253 ± 0.092
0.0AspXaa: 0.0 ± 0.0
Glu
4.357GluAla: 4.357 ± 0.15
0.303GluCys: 0.303 ± 0.029
2.515GluAsp: 2.515 ± 0.09
2.795GluGlu: 2.795 ± 0.118
2.174GluPhe: 2.174 ± 0.084
2.985GluGly: 2.985 ± 0.1
1.152GluHis: 1.152 ± 0.07
3.568GluIle: 3.568 ± 0.126
3.616GluLys: 3.616 ± 0.114
6.099GluLeu: 6.099 ± 0.147
1.265GluMet: 1.265 ± 0.065
2.051GluAsn: 2.051 ± 0.08
1.764GluPro: 1.764 ± 0.068
2.698GluGln: 2.698 ± 0.108
3.335GluArg: 3.335 ± 0.118
3.802GluSer: 3.802 ± 0.108
3.029GluThr: 3.029 ± 0.092
3.796GluVal: 3.796 ± 0.116
0.596GluTrp: 0.596 ± 0.044
1.962GluTyr: 1.962 ± 0.079
0.0GluXaa: 0.0 ± 0.0
Phe
3.559PheAla: 3.559 ± 0.122
0.259PheCys: 0.259 ± 0.027
2.565PheAsp: 2.565 ± 0.096
1.915PheGlu: 1.915 ± 0.093
1.483PhePhe: 1.483 ± 0.076
3.155PheGly: 3.155 ± 0.094
0.666PheHis: 0.666 ± 0.048
2.53PheIle: 2.53 ± 0.107
1.682PheLys: 1.682 ± 0.078
2.896PheLeu: 2.896 ± 0.114
0.921PheMet: 0.921 ± 0.054
1.678PheAsn: 1.678 ± 0.074
1.104PhePro: 1.104 ± 0.054
1.006PheGln: 1.006 ± 0.053
1.612PheArg: 1.612 ± 0.073
2.704PheSer: 2.704 ± 0.098
2.672PheThr: 2.672 ± 0.093
2.937PheVal: 2.937 ± 0.09
0.489PheTrp: 0.489 ± 0.043
1.29PheTyr: 1.29 ± 0.067
0.0PheXaa: 0.0 ± 0.0
Gly
5.764GlyAla: 5.764 ± 0.16
0.429GlyCys: 0.429 ± 0.039
4.168GlyAsp: 4.168 ± 0.124
3.78GlyGlu: 3.78 ± 0.108
2.959GlyPhe: 2.959 ± 0.093
5.581GlyGly: 5.581 ± 0.165
1.385GlyHis: 1.385 ± 0.067
4.969GlyIle: 4.969 ± 0.142
4.266GlyLys: 4.266 ± 0.13
5.872GlyLeu: 5.872 ± 0.153
1.581GlyMet: 1.581 ± 0.077
2.985GlyAsn: 2.985 ± 0.132
1.735GlyPro: 1.735 ± 0.085
2.641GlyGln: 2.641 ± 0.103
3.306GlyArg: 3.306 ± 0.102
4.878GlySer: 4.878 ± 0.153
4.635GlyThr: 4.635 ± 0.138
5.701GlyVal: 5.701 ± 0.143
0.883GlyTrp: 0.883 ± 0.053
2.707GlyTyr: 2.707 ± 0.101
0.0GlyXaa: 0.0 ± 0.0
His
1.615HisAla: 1.615 ± 0.071
0.139HisCys: 0.139 ± 0.019
1.363HisAsp: 1.363 ± 0.06
1.205HisGlu: 1.205 ± 0.064
0.805HisPhe: 0.805 ± 0.059
1.344HisGly: 1.344 ± 0.054
0.669HisHis: 0.669 ± 0.054
1.395HisIle: 1.395 ± 0.066
0.88HisLys: 0.88 ± 0.048
1.565HisLeu: 1.565 ± 0.074
0.476HisMet: 0.476 ± 0.039
0.798HisAsn: 0.798 ± 0.048
0.868HisPro: 0.868 ± 0.057
0.688HisGln: 0.688 ± 0.044
0.956HisArg: 0.956 ± 0.053
1.347HisSer: 1.347 ± 0.074
1.259HisThr: 1.259 ± 0.068
1.227HisVal: 1.227 ± 0.062
0.233HisTrp: 0.233 ± 0.029
0.786HisTyr: 0.786 ± 0.05
0.0HisXaa: 0.0 ± 0.0
Ile
7.194IleAla: 7.194 ± 0.171
0.448IleCys: 0.448 ± 0.044
4.972IleAsp: 4.972 ± 0.136
4.136IleGlu: 4.136 ± 0.124
2.202IlePhe: 2.202 ± 0.094
5.155IleGly: 5.155 ± 0.147
1.212IleHis: 1.212 ± 0.057
4.733IleIle: 4.733 ± 0.141
3.43IleLys: 3.43 ± 0.123
5.364IleLeu: 5.364 ± 0.132
1.306IleMet: 1.306 ± 0.073
2.874IleAsn: 2.874 ± 0.1
2.625IlePro: 2.625 ± 0.1
2.382IleGln: 2.382 ± 0.081
2.994IleArg: 2.994 ± 0.101
4.351IleSer: 4.351 ± 0.122
4.916IleThr: 4.916 ± 0.133
6.051IleVal: 6.051 ± 0.154
0.596IleTrp: 0.596 ± 0.047
1.896IleTyr: 1.896 ± 0.087
0.0IleXaa: 0.0 ± 0.0
Lys
4.502LysAla: 4.502 ± 0.129
0.189LysCys: 0.189 ± 0.023
3.344LysAsp: 3.344 ± 0.123
2.969LysGlu: 2.969 ± 0.105
1.66LysPhe: 1.66 ± 0.075
2.997LysGly: 2.997 ± 0.096
1.06LysHis: 1.06 ± 0.059
3.66LysIle: 3.66 ± 0.107
4.259LysLys: 4.259 ± 0.13
4.928LysLeu: 4.928 ± 0.128
1.461LysMet: 1.461 ± 0.071
2.565LysAsn: 2.565 ± 0.094
2.243LysPro: 2.243 ± 0.085
2.685LysGln: 2.685 ± 0.091
3.051LysArg: 3.051 ± 0.099
3.764LysSer: 3.764 ± 0.113
3.613LysThr: 3.613 ± 0.105
3.884LysVal: 3.884 ± 0.122
0.514LysTrp: 0.514 ± 0.039
1.748LysTyr: 1.748 ± 0.081
0.0LysXaa: 0.0 ± 0.0
Leu
8.55LeuAla: 8.55 ± 0.191
0.549LeuCys: 0.549 ± 0.049
5.061LeuAsp: 5.061 ± 0.129
4.269LeuGlu: 4.269 ± 0.131
3.701LeuPhe: 3.701 ± 0.14
5.913LeuGly: 5.913 ± 0.135
1.871LeuHis: 1.871 ± 0.079
5.461LeuIle: 5.461 ± 0.146
4.376LeuLys: 4.376 ± 0.129
8.664LeuLeu: 8.664 ± 0.262
1.843LeuMet: 1.843 ± 0.067
3.531LeuAsn: 3.531 ± 0.113
4.237LeuPro: 4.237 ± 0.125
3.572LeuGln: 3.572 ± 0.111
4.919LeuArg: 4.919 ± 0.126
6.465LeuSer: 6.465 ± 0.158
5.458LeuThr: 5.458 ± 0.122
6.149LeuVal: 6.149 ± 0.161
0.811LeuTrp: 0.811 ± 0.054
2.754LeuTyr: 2.754 ± 0.1
0.0LeuXaa: 0.0 ± 0.0
Met
2.032MetAla: 2.032 ± 0.08
0.123MetCys: 0.123 ± 0.02
1.063MetAsp: 1.063 ± 0.067
0.95MetGlu: 0.95 ± 0.055
0.656MetPhe: 0.656 ± 0.046
1.262MetGly: 1.262 ± 0.067
0.366MetHis: 0.366 ± 0.037
1.555MetIle: 1.555 ± 0.068
1.521MetLys: 1.521 ± 0.067
1.884MetLeu: 1.884 ± 0.077
0.558MetMet: 0.558 ± 0.04
1.208MetAsn: 1.208 ± 0.06
1.063MetPro: 1.063 ± 0.058
1.038MetGln: 1.038 ± 0.064
1.212MetArg: 1.212 ± 0.067
1.906MetSer: 1.906 ± 0.073
1.609MetThr: 1.609 ± 0.069
1.366MetVal: 1.366 ± 0.077
0.183MetTrp: 0.183 ± 0.023
0.707MetTyr: 0.707 ± 0.046
0.0MetXaa: 0.0 ± 0.0
Asn
3.559AsnAla: 3.559 ± 0.12
0.306AsnCys: 0.306 ± 0.034
2.597AsnAsp: 2.597 ± 0.091
2.373AsnGlu: 2.373 ± 0.095
1.35AsnPhe: 1.35 ± 0.073
3.66AsnGly: 3.66 ± 0.136
0.805AsnHis: 0.805 ± 0.057
2.66AsnIle: 2.66 ± 0.094
2.152AsnLys: 2.152 ± 0.088
3.25AsnLeu: 3.25 ± 0.093
0.849AsnMet: 0.849 ± 0.041
2.01AsnAsn: 2.01 ± 0.096
2.127AsnPro: 2.127 ± 0.084
1.732AsnGln: 1.732 ± 0.076
2.174AsnArg: 2.174 ± 0.09
2.789AsnSer: 2.789 ± 0.12
2.622AsnThr: 2.622 ± 0.109
2.909AsnVal: 2.909 ± 0.097
0.495AsnTrp: 0.495 ± 0.043
1.458AsnTyr: 1.458 ± 0.063
0.0AsnXaa: 0.0 ± 0.0
Pro
3.893ProAla: 3.893 ± 0.145
0.174ProCys: 0.174 ± 0.023
2.492ProAsp: 2.492 ± 0.087
2.726ProGlu: 2.726 ± 0.098
1.495ProPhe: 1.495 ± 0.08
2.641ProGly: 2.641 ± 0.094
0.858ProHis: 0.858 ± 0.053
2.549ProIle: 2.549 ± 0.093
2.209ProLys: 2.209 ± 0.086
3.18ProLeu: 3.18 ± 0.102
0.64ProMet: 0.64 ± 0.052
1.644ProAsn: 1.644 ± 0.079
1.398ProPro: 1.398 ± 0.116
1.66ProGln: 1.66 ± 0.101
1.609ProArg: 1.609 ± 0.075
2.868ProSer: 2.868 ± 0.116
2.874ProThr: 2.874 ± 0.129
3.158ProVal: 3.158 ± 0.119
0.423ProTrp: 0.423 ± 0.038
1.164ProTyr: 1.164 ± 0.067
0.0ProXaa: 0.0 ± 0.0
Gln
3.531GlnAla: 3.531 ± 0.101
0.196GlnCys: 0.196 ± 0.024
1.918GlnAsp: 1.918 ± 0.072
1.754GlnGlu: 1.754 ± 0.071
1.467GlnPhe: 1.467 ± 0.066
2.054GlnGly: 2.054 ± 0.081
0.82GlnHis: 0.82 ± 0.05
2.698GlnIle: 2.698 ± 0.095
2.392GlnLys: 2.392 ± 0.091
4.524GlnLeu: 4.524 ± 0.127
0.95GlnMet: 0.95 ± 0.057
1.751GlnAsn: 1.751 ± 0.069
2.196GlnPro: 2.196 ± 0.128
2.6GlnGln: 2.6 ± 0.127
2.309GlnArg: 2.309 ± 0.098
2.896GlnSer: 2.896 ± 0.102
2.704GlnThr: 2.704 ± 0.091
2.433GlnVal: 2.433 ± 0.083
0.543GlnTrp: 0.543 ± 0.044
1.385GlnTyr: 1.385 ± 0.066
0.0GlnXaa: 0.0 ± 0.0
Arg
4.237ArgAla: 4.237 ± 0.119
0.281ArgCys: 0.281 ± 0.036
3.038ArgAsp: 3.038 ± 0.097
3.086ArgGlu: 3.086 ± 0.113
1.921ArgPhe: 1.921 ± 0.077
2.89ArgGly: 2.89 ± 0.104
1.215ArgHis: 1.215 ± 0.055
3.256ArgIle: 3.256 ± 0.099
2.739ArgLys: 2.739 ± 0.104
4.654ArgLeu: 4.654 ± 0.145
1.388ArgMet: 1.388 ± 0.068
2.057ArgAsn: 2.057 ± 0.081
1.887ArgPro: 1.887 ± 0.072
2.357ArgGln: 2.357 ± 0.087
3.202ArgArg: 3.202 ± 0.118
3.382ArgSer: 3.382 ± 0.109
3.098ArgThr: 3.098 ± 0.109
3.404ArgVal: 3.404 ± 0.109
0.48ArgTrp: 0.48 ± 0.042
1.915ArgTyr: 1.915 ± 0.082
0.0ArgXaa: 0.0 ± 0.0
Ser
5.79SerAla: 5.79 ± 0.133
0.486SerCys: 0.486 ± 0.048
4.398SerAsp: 4.398 ± 0.107
3.701SerGlu: 3.701 ± 0.108
2.72SerPhe: 2.72 ± 0.087
5.543SerGly: 5.543 ± 0.163
1.284SerHis: 1.284 ± 0.065
4.72SerIle: 4.72 ± 0.116
3.534SerLys: 3.534 ± 0.121
5.998SerLeu: 5.998 ± 0.135
1.584SerMet: 1.584 ± 0.077
2.899SerAsn: 2.899 ± 0.118
2.641SerPro: 2.641 ± 0.097
2.776SerGln: 2.776 ± 0.095
3.37SerArg: 3.37 ± 0.099
5.045SerSer: 5.045 ± 0.14
4.256SerThr: 4.256 ± 0.132
5.127SerVal: 5.127 ± 0.143
0.773SerTrp: 0.773 ± 0.059
2.325SerTyr: 2.325 ± 0.081
0.0SerXaa: 0.0 ± 0.0
Thr
6.014ThrAla: 6.014 ± 0.151
0.464ThrCys: 0.464 ± 0.052
3.758ThrAsp: 3.758 ± 0.105
3.013ThrGlu: 3.013 ± 0.103
2.268ThrPhe: 2.268 ± 0.075
4.796ThrGly: 4.796 ± 0.13
1.208ThrHis: 1.208 ± 0.064
5.149ThrIle: 5.149 ± 0.135
3.527ThrLys: 3.527 ± 0.097
6.026ThrLeu: 6.026 ± 0.146
1.183ThrMet: 1.183 ± 0.063
2.827ThrAsn: 2.827 ± 0.105
3.704ThrPro: 3.704 ± 0.158
2.215ThrGln: 2.215 ± 0.094
2.691ThrArg: 2.691 ± 0.085
4.635ThrSer: 4.635 ± 0.138
5.023ThrThr: 5.023 ± 0.17
5.099ThrVal: 5.099 ± 0.126
0.675ThrTrp: 0.675 ± 0.042
2.082ThrTyr: 2.082 ± 0.083
0.0ThrXaa: 0.0 ± 0.0
Val
7.32ValAla: 7.32 ± 0.197
0.508ValCys: 0.508 ± 0.041
4.54ValAsp: 4.54 ± 0.144
4.013ValGlu: 4.013 ± 0.127
2.616ValPhe: 2.616 ± 0.105
5.054ValGly: 5.054 ± 0.114
1.294ValHis: 1.294 ± 0.068
5.682ValIle: 5.682 ± 0.137
3.597ValLys: 3.597 ± 0.122
6.316ValLeu: 6.316 ± 0.139
1.552ValMet: 1.552 ± 0.063
2.855ValAsn: 2.855 ± 0.092
2.593ValPro: 2.593 ± 0.094
2.698ValGln: 2.698 ± 0.1
3.616ValArg: 3.616 ± 0.116
5.124ValSer: 5.124 ± 0.134
5.383ValThr: 5.383 ± 0.127
6.275ValVal: 6.275 ± 0.171
0.735ValTrp: 0.735 ± 0.047
2.246ValTyr: 2.246 ± 0.085
0.0ValXaa: 0.0 ± 0.0
Trp
0.719TrpAla: 0.719 ± 0.054
0.091TrpCys: 0.091 ± 0.016
0.527TrpAsp: 0.527 ± 0.047
0.388TrpGlu: 0.388 ± 0.041
0.464TrpPhe: 0.464 ± 0.04
0.827TrpGly: 0.827 ± 0.078
0.246TrpHis: 0.246 ± 0.028
0.713TrpIle: 0.713 ± 0.052
0.549TrpLys: 0.549 ± 0.044
1.142TrpLeu: 1.142 ± 0.067
0.297TrpMet: 0.297 ± 0.033
0.498TrpAsn: 0.498 ± 0.042
0.429TrpPro: 0.429 ± 0.032
0.685TrpGln: 0.685 ± 0.044
0.732TrpArg: 0.732 ± 0.052
0.767TrpSer: 0.767 ± 0.048
0.571TrpThr: 0.571 ± 0.042
0.704TrpVal: 0.704 ± 0.047
0.227TrpTrp: 0.227 ± 0.023
0.375TrpTyr: 0.375 ± 0.04
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.016TyrAla: 3.016 ± 0.111
0.382TyrCys: 0.382 ± 0.05
2.366TyrAsp: 2.366 ± 0.09
1.89TyrGlu: 1.89 ± 0.083
1.297TyrPhe: 1.297 ± 0.066
2.395TyrGly: 2.395 ± 0.098
0.644TyrHis: 0.644 ± 0.045
1.985TyrIle: 1.985 ± 0.083
1.631TyrLys: 1.631 ± 0.067
2.707TyrLeu: 2.707 ± 0.091
0.644TyrMet: 0.644 ± 0.046
1.518TyrAsn: 1.518 ± 0.087
1.284TyrPro: 1.284 ± 0.062
1.363TyrGln: 1.363 ± 0.074
1.928TyrArg: 1.928 ± 0.083
2.243TyrSer: 2.243 ± 0.084
2.089TyrThr: 2.089 ± 0.085
2.287TyrVal: 2.287 ± 0.083
0.385TyrTrp: 0.385 ± 0.036
1.363TyrTyr: 1.363 ± 0.075
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1223 proteins (316953 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski