Pneumocystis jirovecii (strain RU7) (Human pneumocystis pneumonia agent)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Pneumocystidomycetes; Pneumocystidales; Pneumocystidaceae; Pneumocystis; Pneumocystis jirovecii

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3746 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W4ZV09|A0A0W4ZV09_PNEJ7 4F5 domain-containing protein OS=Pneumocystis jirovecii (strain RU7) OX=1408657 GN=T551_00896 PE=4 SV=1
MM1 pKa = 7.43SFYY4 pKa = 11.25DD5 pKa = 4.0EE6 pKa = 5.34IEE8 pKa = 4.56IEE10 pKa = 5.03DD11 pKa = 3.75FTFDD15 pKa = 3.35TEE17 pKa = 4.2LQIYY21 pKa = 8.98HH22 pKa = 5.9YY23 pKa = 9.19PCPCGDD29 pKa = 3.35RR30 pKa = 11.84FEE32 pKa = 5.82ISLNDD37 pKa = 3.47LRR39 pKa = 11.84KK40 pKa = 9.65GDD42 pKa = 4.37EE43 pKa = 3.73IAYY46 pKa = 9.97CPSCSLMVRR55 pKa = 11.84VIYY58 pKa = 10.58DD59 pKa = 3.06QDD61 pKa = 3.41KK62 pKa = 10.55FLEE65 pKa = 4.34NDD67 pKa = 4.14DD68 pKa = 4.82EE69 pKa = 5.89ISLQSAPFIVVV80 pKa = 3.34

Molecular weight: 9.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W4ZD85|A0A0W4ZD85_PNEJ7 Ubiquitin-60S ribosomal protein L40 OS=Pneumocystis jirovecii (strain RU7) OX=1408657 GN=T551_03569 PE=4 SV=1
MM1 pKa = 7.44SLHH4 pKa = 6.81ALHH7 pKa = 7.0ALHH10 pKa = 7.17ASHH13 pKa = 6.83APKK16 pKa = 10.26RR17 pKa = 11.84ALFLRR22 pKa = 11.84GPSSSPALHH31 pKa = 7.15RR32 pKa = 11.84ILADD36 pKa = 4.16LSALAPDD43 pKa = 4.15SVSLSRR49 pKa = 11.84PNALYY54 pKa = 10.03PFDD57 pKa = 5.79DD58 pKa = 4.35PASLEE63 pKa = 4.03FLAHH67 pKa = 6.57KK68 pKa = 10.24NHH70 pKa = 6.67AALVVVASSSRR81 pKa = 11.84KK82 pKa = 9.82RR83 pKa = 11.84GDD85 pKa = 3.22VLIFARR91 pKa = 11.84LFDD94 pKa = 4.17GALLDD99 pKa = 3.8MLEE102 pKa = 4.58LCLSDD107 pKa = 3.73AHH109 pKa = 6.41PMHH112 pKa = 6.95LFSAKK117 pKa = 9.97SPCGLRR123 pKa = 11.84PLLLFAGAPFDD134 pKa = 3.63VSPPHH139 pKa = 6.57RR140 pKa = 11.84LCSSLFLDD148 pKa = 5.02LFGGPLPRR156 pKa = 11.84IHH158 pKa = 7.67ADD160 pKa = 3.35GLQHH164 pKa = 7.0AVCLTATDD172 pKa = 3.47SHH174 pKa = 6.54AHH176 pKa = 5.23LRR178 pKa = 11.84VYY180 pKa = 7.4TLCRR184 pKa = 11.84GATSVSLCEE193 pKa = 4.02AGPRR197 pKa = 11.84LDD199 pKa = 3.59FTLGRR204 pKa = 11.84VRR206 pKa = 11.84EE207 pKa = 4.01ASAAQLHH214 pKa = 6.54AATAPAAVPRR224 pKa = 11.84PPRR227 pKa = 11.84NTSLSSRR234 pKa = 11.84GDD236 pKa = 3.07RR237 pKa = 11.84VGRR240 pKa = 11.84VHH242 pKa = 6.63VAQRR246 pKa = 11.84LSLQTRR252 pKa = 11.84KK253 pKa = 9.28FRR255 pKa = 11.84ALRR258 pKa = 11.84ARR260 pKa = 11.84RR261 pKa = 11.84TGGG264 pKa = 3.07

Molecular weight: 28.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3746

0

3746

1771070

46

5096

472.8

54.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.512 ± 0.031

1.885 ± 0.029

5.381 ± 0.022

6.987 ± 0.041

4.706 ± 0.03

4.306 ± 0.036

2.228 ± 0.015

8.059 ± 0.051

8.575 ± 0.055

9.864 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.054 ± 0.013

6.179 ± 0.034

3.656 ± 0.03

3.701 ± 0.025

4.395 ± 0.025

8.538 ± 0.04

5.321 ± 0.031

4.957 ± 0.026

1.062 ± 0.011

3.634 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski