Amino acid dipepetide frequency for Pneumocystis jirovecii (strain RU7) (Human pneumocystis pneumonia agent)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	2.742AlaAla: 2.742 ± 0.054	0.984AlaCys: 0.984 ± 0.028	1.838AlaAsp: 1.838 ± 0.035	2.78AlaGlu: 2.78 ± 0.045	2.312AlaPhe: 2.312 ± 0.035	2.03AlaGly: 2.03 ± 0.043	0.948AlaHis: 0.948 ± 0.025	3.579AlaIle: 3.579 ± 0.054	3.327AlaLys: 3.327 ± 0.049	5.261AlaLeu: 5.261 ± 0.074	1.052AlaMet: 1.052 ± 0.028	2.128AlaAsn: 2.128 ± 0.033	1.526AlaPro: 1.526 ± 0.033	1.667AlaGln: 1.667 ± 0.036	2.175AlaArg: 2.175 ± 0.042	3.797AlaSer: 3.797 ± 0.051	2.323AlaThr: 2.323 ± 0.039	2.528AlaVal: 2.528 ± 0.042	0.517AlaTrp: 0.517 ± 0.018	1.606AlaTyr: 1.606 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	0.738CysAla: 0.738 ± 0.025	0.266CysCys: 0.266 ± 0.015	0.941CysAsp: 0.941 ± 0.028	1.213CysGlu: 1.213 ± 0.043	1.012CysPhe: 1.012 ± 0.025	0.912CysGly: 0.912 ± 0.024	0.483CysHis: 0.483 ± 0.017	1.51CysIle: 1.51 ± 0.026	1.911CysLys: 1.911 ± 0.079	1.895CysLeu: 1.895 ± 0.038	0.341CysMet: 0.341 ± 0.014	1.102CysAsn: 1.102 ± 0.033	0.719CysPro: 0.719 ± 0.021	0.671CysGln: 0.671 ± 0.02	0.772CysArg: 0.772 ± 0.022	1.508CysSer: 1.508 ± 0.034	1.026CysThr: 1.026 ± 0.033	0.912CysVal: 0.912 ± 0.023	0.15CysTrp: 0.15 ± 0.009	0.765CysTyr: 0.765 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	2.51AspAla: 2.51 ± 0.042	0.983AspCys: 0.983 ± 0.032	3.459AspAsp: 3.459 ± 0.059	3.987AspGlu: 3.987 ± 0.049	2.814AspPhe: 2.814 ± 0.039	2.243AspGly: 2.243 ± 0.047	1.026AspHis: 1.026 ± 0.022	5.267AspIle: 5.267 ± 0.06	3.844AspLys: 3.844 ± 0.048	5.377AspLeu: 5.377 ± 0.065	1.156AspMet: 1.156 ± 0.025	2.966AspAsn: 2.966 ± 0.049	2.081AspPro: 2.081 ± 0.039	1.668AspGln: 1.668 ± 0.031	1.878AspArg: 1.878 ± 0.036	4.299AspSer: 4.299 ± 0.058	2.875AspThr: 2.875 ± 0.046	2.91AspVal: 2.91 ± 0.049	0.556AspTrp: 0.556 ± 0.018	1.908AspTyr: 1.908 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	3.3GluAla: 3.3 ± 0.051	1.196GluCys: 1.196 ± 0.037	3.708GluAsp: 3.708 ± 0.055	5.736GluGlu: 5.736 ± 0.09	2.714GluPhe: 2.714 ± 0.04	2.484GluGly: 2.484 ± 0.046	1.43GluHis: 1.43 ± 0.03	5.705GluIle: 5.705 ± 0.068	7.899GluLys: 7.899 ± 0.108	6.547GluLeu: 6.547 ± 0.085	1.51GluMet: 1.51 ± 0.031	5.581GluAsn: 5.581 ± 0.073	1.794GluPro: 1.794 ± 0.054	2.361GluGln: 2.361 ± 0.048	3.119GluArg: 3.119 ± 0.052	4.816GluSer: 4.816 ± 0.057	3.866GluThr: 3.866 ± 0.053	2.882GluVal: 2.882 ± 0.044	0.758GluTrp: 0.758 ± 0.023	2.459GluTyr: 2.459 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	1.739PheAla: 1.739 ± 0.035	0.906PheCys: 0.906 ± 0.024	2.651PheAsp: 2.651 ± 0.042	2.905PheGlu: 2.905 ± 0.042	2.644PhePhe: 2.644 ± 0.059	2.193PheGly: 2.193 ± 0.046	1.118PheHis: 1.118 ± 0.026	3.575PheIle: 3.575 ± 0.056	3.316PheLys: 3.316 ± 0.045	5.035PheLeu: 5.035 ± 0.063	0.975PheMet: 0.975 ± 0.024	2.489PheAsn: 2.489 ± 0.045	1.872PhePro: 1.872 ± 0.033	1.908PheGln: 1.908 ± 0.032	1.891PheArg: 1.891 ± 0.036	5.056PheSer: 5.056 ± 0.063	2.001PheThr: 2.001 ± 0.039	2.36PheVal: 2.36 ± 0.04	0.562PheTrp: 0.562 ± 0.018	1.862PheTyr: 1.862 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	1.988GlyAla: 1.988 ± 0.044	0.727GlyCys: 0.727 ± 0.023	2.065GlyAsp: 2.065 ± 0.038	2.357GlyGlu: 2.357 ± 0.04	2.23GlyPhe: 2.23 ± 0.038	2.37GlyGly: 2.37 ± 0.057	1.084GlyHis: 1.084 ± 0.029	3.782GlyIle: 3.782 ± 0.057	3.65GlyLys: 3.65 ± 0.047	4.162GlyLeu: 4.162 ± 0.053	0.924GlyMet: 0.924 ± 0.026	2.309GlyAsn: 2.309 ± 0.04	1.32GlyPro: 1.32 ± 0.031	1.344GlyGln: 1.344 ± 0.031	2.118GlyArg: 2.118 ± 0.046	3.409GlySer: 3.409 ± 0.055	2.398GlyThr: 2.398 ± 0.048	2.504GlyVal: 2.504 ± 0.045	0.626GlyTrp: 0.626 ± 0.02	1.691GlyTyr: 1.691 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	0.997HisAla: 0.997 ± 0.027	0.424HisCys: 0.424 ± 0.016	1.154HisAsp: 1.154 ± 0.024	1.475HisGlu: 1.475 ± 0.03	1.123HisPhe: 1.123 ± 0.027	1.037HisGly: 1.037 ± 0.025	0.586HisHis: 0.586 ± 0.018	1.886HisIle: 1.886 ± 0.034	1.526HisLys: 1.526 ± 0.031	2.279HisLeu: 2.279 ± 0.036	0.452HisMet: 0.452 ± 0.017	1.169HisAsn: 1.169 ± 0.028	1.065HisPro: 1.065 ± 0.026	0.809HisGln: 0.809 ± 0.022	0.99HisArg: 0.99 ± 0.023	1.88HisSer: 1.88 ± 0.035	1.204HisThr: 1.204 ± 0.023	1.215HisVal: 1.215 ± 0.023	0.244HisTrp: 0.244 ± 0.011	0.768HisTyr: 0.768 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	3.491IleAla: 3.491 ± 0.051	1.535IleCys: 1.535 ± 0.038	4.426IleAsp: 4.426 ± 0.061	5.437IleGlu: 5.437 ± 0.069	4.026IlePhe: 4.026 ± 0.064	3.314IleGly: 3.314 ± 0.056	1.92IleHis: 1.92 ± 0.036	6.288IleIle: 6.288 ± 0.085	6.289IleLys: 6.289 ± 0.066	8.285IleLeu: 8.285 ± 0.092	1.643IleMet: 1.643 ± 0.03	4.773IleAsn: 4.773 ± 0.068	3.251IlePro: 3.251 ± 0.05	3.248IleGln: 3.248 ± 0.042	3.371IleArg: 3.371 ± 0.042	7.567IleSer: 7.567 ± 0.08	3.841IleThr: 3.841 ± 0.05	3.923IleVal: 3.923 ± 0.054	0.873IleTrp: 0.873 ± 0.024	3.09IleTyr: 3.09 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	3.663LysAla: 3.663 ± 0.054	1.867LysCys: 1.867 ± 0.073	4.931LysAsp: 4.931 ± 0.064	7.126LysGlu: 7.126 ± 0.109	2.869LysPhe: 2.869 ± 0.04	3.052LysGly: 3.052 ± 0.043	1.941LysHis: 1.941 ± 0.035	6.905LysIle: 6.905 ± 0.07	9.473LysLys: 9.473 ± 0.12	7.216LysLeu: 7.216 ± 0.082	1.771LysMet: 1.771 ± 0.032	6.804LysAsn: 6.804 ± 0.09	2.594LysPro: 2.594 ± 0.042	3.035LysGln: 3.035 ± 0.046	3.961LysArg: 3.961 ± 0.052	5.983LysSer: 5.983 ± 0.064	4.972LysThr: 4.972 ± 0.062	3.625LysVal: 3.625 ± 0.048	0.809LysTrp: 0.809 ± 0.021	3.156LysTyr: 3.156 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	4.437LeuAla: 4.437 ± 0.056	1.952LeuCys: 1.952 ± 0.043	5.379LeuAsp: 5.379 ± 0.056	7.624LeuGlu: 7.624 ± 0.105	4.585LeuPhe: 4.585 ± 0.066	4.091LeuGly: 4.091 ± 0.049	2.156LeuHis: 2.156 ± 0.033	6.525LeuIle: 6.525 ± 0.081	9.263LeuLys: 9.263 ± 0.104	9.452LeuLeu: 9.452 ± 0.104	1.815LeuMet: 1.815 ± 0.032	5.991LeuAsn: 5.991 ± 0.057	3.754LeuPro: 3.754 ± 0.052	3.951LeuGln: 3.951 ± 0.056	4.767LeuArg: 4.767 ± 0.056	9.092LeuSer: 9.092 ± 0.077	4.555LeuThr: 4.555 ± 0.053	4.585LeuVal: 4.585 ± 0.061	1.113LeuTrp: 1.113 ± 0.029	3.554LeuTyr: 3.554 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	1.06MetAla: 1.06 ± 0.028	0.285MetCys: 0.285 ± 0.013	1.22MetAsp: 1.22 ± 0.029	1.5MetGlu: 1.5 ± 0.028	0.873MetPhe: 0.873 ± 0.02	0.926MetGly: 0.926 ± 0.029	0.496MetHis: 0.496 ± 0.018	1.567MetIle: 1.567 ± 0.031	1.721MetLys: 1.721 ± 0.035	1.925MetLeu: 1.925 ± 0.035	0.433MetMet: 0.433 ± 0.015	1.276MetAsn: 1.276 ± 0.023	0.756MetPro: 0.756 ± 0.024	0.853MetGln: 0.853 ± 0.028	0.938MetArg: 0.938 ± 0.026	1.816MetSer: 1.816 ± 0.033	1.056MetThr: 1.056 ± 0.027	1.055MetVal: 1.055 ± 0.027	0.15MetTrp: 0.15 ± 0.01	0.635MetTyr: 0.635 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.633AsnAla: 2.633 ± 0.037	1.037AsnCys: 1.037 ± 0.03	3.576AsnAsp: 3.576 ± 0.055	4.408AsnGlu: 4.408 ± 0.062	2.69AsnPhe: 2.69 ± 0.049	2.478AsnGly: 2.478 ± 0.042	1.265AsnHis: 1.265 ± 0.024	6.28AsnIle: 6.28 ± 0.074	5.112AsnLys: 5.112 ± 0.069	5.443AsnLeu: 5.443 ± 0.066	1.4AsnMet: 1.4 ± 0.031	4.458AsnAsn: 4.458 ± 0.06	2.133AsnPro: 2.133 ± 0.039	2.127AsnGln: 2.127 ± 0.036	2.002AsnArg: 2.002 ± 0.033	5.078AsnSer: 5.078 ± 0.071	3.872AsnThr: 3.872 ± 0.055	3.096AsnVal: 3.096 ± 0.045	0.523AsnTrp: 0.523 ± 0.019	2.175AsnTyr: 2.175 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	1.437ProAla: 1.437 ± 0.032	0.576ProCys: 0.576 ± 0.016	1.84ProAsp: 1.84 ± 0.036	2.62ProGlu: 2.62 ± 0.054	1.79ProPhe: 1.79 ± 0.037	1.633ProGly: 1.633 ± 0.037	0.824ProHis: 0.824 ± 0.023	2.754ProIle: 2.754 ± 0.048	2.663ProLys: 2.663 ± 0.043	3.663ProLeu: 3.663 ± 0.047	0.634ProMet: 0.634 ± 0.023	1.926ProAsn: 1.926 ± 0.038	2.028ProPro: 2.028 ± 0.074	1.314ProGln: 1.314 ± 0.036	1.444ProArg: 1.444 ± 0.038	3.515ProSer: 3.515 ± 0.058	2.032ProThr: 2.032 ± 0.038	2.001ProVal: 2.001 ± 0.038	0.387ProTrp: 0.387 ± 0.016	1.48ProTyr: 1.48 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	1.749GlnAla: 1.749 ± 0.036	0.677GlnCys: 0.677 ± 0.024	1.853GlnAsp: 1.853 ± 0.03	2.867GlnGlu: 2.867 ± 0.043	1.418GlnPhe: 1.418 ± 0.026	1.444GlnGly: 1.444 ± 0.033	0.806GlnHis: 0.806 ± 0.02	2.759GlnIle: 2.759 ± 0.042	3.743GlnLys: 3.743 ± 0.055	3.37GlnLeu: 3.37 ± 0.048	0.785GlnMet: 0.785 ± 0.021	2.752GlnAsn: 2.752 ± 0.049	1.185GlnPro: 1.185 ± 0.03	1.661GlnGln: 1.661 ± 0.049	1.801GlnArg: 1.801 ± 0.036	2.683GlnSer: 2.683 ± 0.042	2.09GlnThr: 2.09 ± 0.031	1.671GlnVal: 1.671 ± 0.031	0.4GlnTrp: 0.4 ± 0.014	1.296GlnTyr: 1.296 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	2.31ArgAla: 2.31 ± 0.044	0.82ArgCys: 0.82 ± 0.023	2.176ArgAsp: 2.176 ± 0.037	2.732ArgGlu: 2.732 ± 0.052	1.976ArgPhe: 1.976 ± 0.037	2.07ArgGly: 2.07 ± 0.051	1.023ArgHis: 1.023 ± 0.029	3.548ArgIle: 3.548 ± 0.049	3.881ArgLys: 3.881 ± 0.055	4.387ArgLeu: 4.387 ± 0.056	0.969ArgMet: 0.969 ± 0.025	2.502ArgAsn: 2.502 ± 0.041	1.514ArgPro: 1.514 ± 0.036	1.725ArgGln: 1.725 ± 0.032	2.745ArgArg: 2.745 ± 0.053	3.174ArgSer: 3.174 ± 0.056	2.133ArgThr: 2.133 ± 0.039	2.234ArgVal: 2.234 ± 0.039	0.509ArgTrp: 0.509 ± 0.018	1.523ArgTyr: 1.523 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	3.446SerAla: 3.446 ± 0.048	1.451SerCys: 1.451 ± 0.033	4.502SerAsp: 4.502 ± 0.055	5.55SerGlu: 5.55 ± 0.058	4.41SerPhe: 4.41 ± 0.057	3.839SerGly: 3.839 ± 0.055	1.722SerHis: 1.722 ± 0.03	6.847SerIle: 6.847 ± 0.08	6.871SerLys: 6.871 ± 0.069	8.849SerLeu: 8.849 ± 0.086	1.637SerMet: 1.637 ± 0.028	5.136SerAsn: 5.136 ± 0.069	3.133SerPro: 3.133 ± 0.062	3.151SerGln: 3.151 ± 0.043	3.565SerArg: 3.565 ± 0.062	8.42SerSer: 8.42 ± 0.112	4.817SerThr: 4.817 ± 0.074	4.203SerVal: 4.203 ± 0.046	0.87SerTrp: 0.87 ± 0.024	2.959SerTyr: 2.959 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	2.36ThrAla: 2.36 ± 0.039	1.125ThrCys: 1.125 ± 0.029	2.609ThrAsp: 2.609 ± 0.042	3.347ThrGlu: 3.347 ± 0.045	2.465ThrPhe: 2.465 ± 0.04	2.497ThrGly: 2.497 ± 0.041	1.276ThrHis: 1.276 ± 0.032	4.053ThrIle: 4.053 ± 0.056	4.002ThrLys: 4.002 ± 0.053	5.472ThrLeu: 5.472 ± 0.06	1.01ThrMet: 1.01 ± 0.025	3.038ThrAsn: 3.038 ± 0.053	2.22ThrPro: 2.22 ± 0.046	2.019ThrGln: 2.019 ± 0.034	2.168ThrArg: 2.168 ± 0.036	4.95ThrSer: 4.95 ± 0.066	3.376ThrThr: 3.376 ± 0.076	2.8ThrVal: 2.8 ± 0.037	0.587ThrTrp: 0.587 ± 0.018	1.842ThrTyr: 1.842 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	2.461ValAla: 2.461 ± 0.04	0.991ValCys: 0.991 ± 0.029	2.688ValAsp: 2.688 ± 0.043	3.217ValGlu: 3.217 ± 0.048	2.78ValPhe: 2.78 ± 0.04	2.112ValGly: 2.112 ± 0.044	1.072ValHis: 1.072 ± 0.026	3.674ValIle: 3.674 ± 0.053	3.676ValLys: 3.676 ± 0.046	5.457ValLeu: 5.457 ± 0.066	1.017ValMet: 1.017 ± 0.025	2.591ValAsn: 2.591 ± 0.034	1.967ValPro: 1.967 ± 0.037	1.817ValGln: 1.817 ± 0.031	2.16ValArg: 2.16 ± 0.033	4.524ValSer: 4.524 ± 0.056	2.4ValThr: 2.4 ± 0.044	2.667ValVal: 2.667 ± 0.044	0.533ValTrp: 0.533 ± 0.02	1.77ValTyr: 1.77 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.507TrpAla: 0.507 ± 0.016	0.191TrpCys: 0.191 ± 0.011	0.687TrpAsp: 0.687 ± 0.023	0.671TrpGlu: 0.671 ± 0.022	0.423TrpPhe: 0.423 ± 0.017	0.496TrpGly: 0.496 ± 0.016	0.231TrpHis: 0.231 ± 0.011	0.925TrpIle: 0.925 ± 0.027	0.964TrpLys: 0.964 ± 0.026	0.898TrpLeu: 0.898 ± 0.023	0.25TrpMet: 0.25 ± 0.012	0.671TrpAsn: 0.671 ± 0.021	0.353TrpPro: 0.353 ± 0.014	0.305TrpGln: 0.305 ± 0.013	0.536TrpArg: 0.536 ± 0.019	0.843TrpSer: 0.843 ± 0.023	0.552TrpThr: 0.552 ± 0.019	0.632TrpVal: 0.632 ± 0.022	0.143TrpTrp: 0.143 ± 0.009	0.344TrpTyr: 0.344 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.552TyrAla: 1.552 ± 0.033	0.853TyrCys: 0.853 ± 0.027	2.103TyrAsp: 2.103 ± 0.04	2.316TyrGlu: 2.316 ± 0.039	1.904TyrPhe: 1.904 ± 0.035	1.838TyrGly: 1.838 ± 0.035	0.876TyrHis: 0.876 ± 0.023	3.162TyrIle: 3.162 ± 0.049	2.624TyrLys: 2.624 ± 0.043	3.667TyrLeu: 3.667 ± 0.055	0.767TyrMet: 0.767 ± 0.023	2.125TyrAsn: 2.125 ± 0.041	1.296TyrPro: 1.296 ± 0.027	1.377TyrGln: 1.377 ± 0.029	1.551TyrArg: 1.551 ± 0.033	2.964TyrSer: 2.964 ± 0.049	1.825TyrThr: 1.825 ± 0.033	1.772TyrVal: 1.772 ± 0.034	0.312TyrTrp: 0.312 ± 0.014	1.451TyrTyr: 1.451 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3746 proteins (1771070 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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