Amino acid dipepetide frequency for Methylobacterium tarhaniae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.152AlaAla: 23.152 ± 0.215
1.382AlaCys: 1.382 ± 0.031
6.915AlaAsp: 6.915 ± 0.08
9.065AlaGlu: 9.065 ± 0.077
4.731AlaPhe: 4.731 ± 0.054
13.537AlaGly: 13.537 ± 0.099
2.418AlaHis: 2.418 ± 0.043
5.721AlaIle: 5.721 ± 0.062
3.415AlaLys: 3.415 ± 0.056
16.541AlaLeu: 16.541 ± 0.12
3.521AlaMet: 3.521 ± 0.048
2.301AlaAsn: 2.301 ± 0.044
7.423AlaPro: 7.423 ± 0.09
4.139AlaGln: 4.139 ± 0.053
11.826AlaArg: 11.826 ± 0.102
6.524AlaSer: 6.524 ± 0.068
6.739AlaThr: 6.739 ± 0.074
10.806AlaVal: 10.806 ± 0.086
1.867AlaTrp: 1.867 ± 0.038
2.823AlaTyr: 2.823 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
1.057CysAla: 1.057 ± 0.025
0.095CysCys: 0.095 ± 0.008
0.488CysAsp: 0.488 ± 0.016
0.394CysGlu: 0.394 ± 0.017
0.259CysPhe: 0.259 ± 0.012
0.92CysGly: 0.92 ± 0.025
0.202CysHis: 0.202 ± 0.012
0.319CysIle: 0.319 ± 0.015
0.124CysLys: 0.124 ± 0.009
0.922CysLeu: 0.922 ± 0.026
0.119CysMet: 0.119 ± 0.008
0.16CysAsn: 0.16 ± 0.01
0.491CysPro: 0.491 ± 0.019
0.175CysGln: 0.175 ± 0.011
0.736CysArg: 0.736 ± 0.023
0.345CysSer: 0.345 ± 0.014
0.443CysThr: 0.443 ± 0.015
0.579CysVal: 0.579 ± 0.019
0.101CysTrp: 0.101 ± 0.009
0.15CysTyr: 0.15 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.371AspAla: 7.371 ± 0.072
0.395AspCys: 0.395 ± 0.016
2.844AspAsp: 2.844 ± 0.05
3.296AspGlu: 3.296 ± 0.053
1.842AspPhe: 1.842 ± 0.036
5.59AspGly: 5.59 ± 0.077
1.111AspHis: 1.111 ± 0.026
2.109AspIle: 2.109 ± 0.041
1.158AspLys: 1.158 ± 0.032
6.717AspLeu: 6.717 ± 0.078
0.862AspMet: 0.862 ± 0.025
0.83AspAsn: 0.83 ± 0.02
4.217AspPro: 4.217 ± 0.057
1.427AspGln: 1.427 ± 0.029
4.827AspArg: 4.827 ± 0.063
1.675AspSer: 1.675 ± 0.031
2.387AspThr: 2.387 ± 0.04
3.958AspVal: 3.958 ± 0.052
0.834AspTrp: 0.834 ± 0.024
1.264AspTyr: 1.264 ± 0.026
0.0AspXaa: 0.0 ± 0.0
Glu
9.896GluAla: 9.896 ± 0.089
0.273GluCys: 0.273 ± 0.012
2.425GluAsp: 2.425 ± 0.043
2.983GluGlu: 2.983 ± 0.049
1.326GluPhe: 1.326 ± 0.029
4.719GluGly: 4.719 ± 0.056
1.066GluHis: 1.066 ± 0.026
3.013GluIle: 3.013 ± 0.043
1.375GluLys: 1.375 ± 0.033
4.134GluLeu: 4.134 ± 0.053
1.245GluMet: 1.245 ± 0.026
1.089GluAsn: 1.089 ± 0.024
3.157GluPro: 3.157 ± 0.047
1.565GluGln: 1.565 ± 0.033
5.723GluArg: 5.723 ± 0.07
2.166GluSer: 2.166 ± 0.041
3.542GluThr: 3.542 ± 0.043
3.991GluVal: 3.991 ± 0.048
0.593GluTrp: 0.593 ± 0.019
0.766GluTyr: 0.766 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
4.495PheAla: 4.495 ± 0.05
0.361PheCys: 0.361 ± 0.015
2.123PheAsp: 2.123 ± 0.039
1.769PheGlu: 1.769 ± 0.032
1.137PhePhe: 1.137 ± 0.032
3.533PheGly: 3.533 ± 0.054
0.614PheHis: 0.614 ± 0.021
1.091PheIle: 1.091 ± 0.028
0.681PheLys: 0.681 ± 0.018
3.267PheLeu: 3.267 ± 0.053
0.571PheMet: 0.571 ± 0.018
0.753PheAsn: 0.753 ± 0.022
1.584PhePro: 1.584 ± 0.031
0.869PheGln: 0.869 ± 0.022
2.463PheArg: 2.463 ± 0.041
1.863PheSer: 1.863 ± 0.033
1.911PheThr: 1.911 ± 0.035
2.667PheVal: 2.667 ± 0.044
0.451PheTrp: 0.451 ± 0.017
0.763PheTyr: 0.763 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
11.298GlyAla: 11.298 ± 0.078
0.823GlyCys: 0.823 ± 0.023
4.381GlyAsp: 4.381 ± 0.062
4.955GlyGlu: 4.955 ± 0.059
3.56GlyPhe: 3.56 ± 0.045
8.106GlyGly: 8.106 ± 0.113
2.014GlyHis: 2.014 ± 0.036
4.321GlyIle: 4.321 ± 0.06
2.342GlyLys: 2.342 ± 0.042
11.189GlyLeu: 11.189 ± 0.1
2.033GlyMet: 2.033 ± 0.034
1.721GlyAsn: 1.721 ± 0.047
4.924GlyPro: 4.924 ± 0.057
2.899GlyGln: 2.899 ± 0.042
8.329GlyArg: 8.329 ± 0.08
4.609GlySer: 4.609 ± 0.058
5.224GlyThr: 5.224 ± 0.066
6.255GlyVal: 6.255 ± 0.061
1.373GlyTrp: 1.373 ± 0.029
2.215GlyTyr: 2.215 ± 0.041
0.0GlyXaa: 0.0 ± 0.0
His
2.58HisAla: 2.58 ± 0.041
0.201HisCys: 0.201 ± 0.012
1.208HisAsp: 1.208 ± 0.025
0.947HisGlu: 0.947 ± 0.024
0.689HisPhe: 0.689 ± 0.019
2.084HisGly: 2.084 ± 0.034
0.58HisHis: 0.58 ± 0.022
0.626HisIle: 0.626 ± 0.02
0.349HisLys: 0.349 ± 0.013
2.182HisLeu: 2.182 ± 0.036
0.352HisMet: 0.352 ± 0.016
0.363HisAsn: 0.363 ± 0.015
1.517HisPro: 1.517 ± 0.032
0.496HisGln: 0.496 ± 0.016
1.681HisArg: 1.681 ± 0.032
0.698HisSer: 0.698 ± 0.02
0.717HisThr: 0.717 ± 0.02
1.452HisVal: 1.452 ± 0.032
0.295HisTrp: 0.295 ± 0.014
0.495HisTyr: 0.495 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
6.498IleAla: 6.498 ± 0.066
0.368IleCys: 0.368 ± 0.012
2.932IleAsp: 2.932 ± 0.045
2.859IleGlu: 2.859 ± 0.042
1.125IlePhe: 1.125 ± 0.027
4.55IleGly: 4.55 ± 0.061
0.766IleHis: 0.766 ± 0.023
1.281IleIle: 1.281 ± 0.032
0.926IleLys: 0.926 ± 0.025
4.036IleLeu: 4.036 ± 0.056
0.607IleMet: 0.607 ± 0.019
0.944IleAsn: 0.944 ± 0.027
2.09IlePro: 2.09 ± 0.039
1.03IleGln: 1.03 ± 0.024
3.116IleArg: 3.116 ± 0.042
1.742IleSer: 1.742 ± 0.038
2.177IleThr: 2.177 ± 0.035
3.707IleVal: 3.707 ± 0.049
0.427IleTrp: 0.427 ± 0.016
0.821IleTyr: 0.821 ± 0.024
0.0IleXaa: 0.0 ± 0.0
Lys
3.635LysAla: 3.635 ± 0.054
0.095LysCys: 0.095 ± 0.009
1.22LysAsp: 1.22 ± 0.03
1.068LysGlu: 1.068 ± 0.029
0.564LysPhe: 0.564 ± 0.02
2.154LysGly: 2.154 ± 0.039
0.355LysHis: 0.355 ± 0.015
1.013LysIle: 1.013 ± 0.027
0.688LysLys: 0.688 ± 0.025
2.178LysLeu: 2.178 ± 0.038
0.434LysMet: 0.434 ± 0.017
0.525LysAsn: 0.525 ± 0.018
1.814LysPro: 1.814 ± 0.037
0.62LysGln: 0.62 ± 0.02
1.859LysArg: 1.859 ± 0.032
1.152LysSer: 1.152 ± 0.027
1.371LysThr: 1.371 ± 0.034
1.891LysVal: 1.891 ± 0.042
0.213LysTrp: 0.213 ± 0.01
0.398LysTyr: 0.398 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
17.465LeuAla: 17.465 ± 0.145
0.967LeuCys: 0.967 ± 0.023
6.783LeuAsp: 6.783 ± 0.067
4.589LeuGlu: 4.589 ± 0.055
3.385LeuPhe: 3.385 ± 0.055
9.306LeuGly: 9.306 ± 0.084
2.008LeuHis: 2.008 ± 0.038
4.307LeuIle: 4.307 ± 0.06
2.735LeuLys: 2.735 ± 0.04
9.841LeuLeu: 9.841 ± 0.105
2.124LeuMet: 2.124 ± 0.032
2.093LeuAsn: 2.093 ± 0.039
6.401LeuPro: 6.401 ± 0.068
2.415LeuGln: 2.415 ± 0.042
8.277LeuArg: 8.277 ± 0.074
6.08LeuSer: 6.08 ± 0.067
6.023LeuThr: 6.023 ± 0.065
8.818LeuVal: 8.818 ± 0.079
1.182LeuTrp: 1.182 ± 0.026
1.989LeuTyr: 1.989 ± 0.035
0.0LeuXaa: 0.0 ± 0.0
Met
2.772MetAla: 2.772 ± 0.043
0.111MetCys: 0.111 ± 0.009
0.799MetAsp: 0.799 ± 0.016
0.823MetGlu: 0.823 ± 0.022
0.484MetPhe: 0.484 ± 0.02
1.509MetGly: 1.509 ± 0.03
0.359MetHis: 0.359 ± 0.016
0.995MetIle: 0.995 ± 0.025
0.585MetLys: 0.585 ± 0.019
2.122MetLeu: 2.122 ± 0.041
0.514MetMet: 0.514 ± 0.019
0.552MetAsn: 0.552 ± 0.018
1.535MetPro: 1.535 ± 0.032
0.693MetGln: 0.693 ± 0.019
1.933MetArg: 1.933 ± 0.028
1.315MetSer: 1.315 ± 0.026
1.592MetThr: 1.592 ± 0.033
1.438MetVal: 1.438 ± 0.026
0.146MetTrp: 0.146 ± 0.01
0.222MetTyr: 0.222 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
2.648AsnAla: 2.648 ± 0.044
0.169AsnCys: 0.169 ± 0.01
1.035AsnAsp: 1.035 ± 0.031
0.851AsnGlu: 0.851 ± 0.023
0.657AsnPhe: 0.657 ± 0.021
1.876AsnGly: 1.876 ± 0.046
0.369AsnHis: 0.369 ± 0.014
0.772AsnIle: 0.772 ± 0.023
0.431AsnLys: 0.431 ± 0.017
2.175AsnLeu: 2.175 ± 0.032
0.372AsnMet: 0.372 ± 0.015
0.471AsnAsn: 0.471 ± 0.019
1.65AsnPro: 1.65 ± 0.03
0.576AsnGln: 0.576 ± 0.02
1.608AsnArg: 1.608 ± 0.032
0.754AsnSer: 0.754 ± 0.021
0.926AsnThr: 0.926 ± 0.025
1.52AsnVal: 1.52 ± 0.031
0.28AsnTrp: 0.28 ± 0.013
0.48AsnTyr: 0.48 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
8.554ProAla: 8.554 ± 0.088
0.359ProCys: 0.359 ± 0.016
4.281ProAsp: 4.281 ± 0.053
4.39ProGlu: 4.39 ± 0.06
2.044ProPhe: 2.044 ± 0.035
6.028ProGly: 6.028 ± 0.072
1.177ProHis: 1.177 ± 0.026
2.051ProIle: 2.051 ± 0.038
1.416ProLys: 1.416 ± 0.032
5.411ProLeu: 5.411 ± 0.061
1.168ProMet: 1.168 ± 0.022
1.194ProAsn: 1.194 ± 0.027
3.395ProPro: 3.395 ± 0.062
1.553ProGln: 1.553 ± 0.031
3.954ProArg: 3.954 ± 0.057
2.823ProSer: 2.823 ± 0.043
2.58ProThr: 2.58 ± 0.045
5.1ProVal: 5.1 ± 0.067
0.787ProTrp: 0.787 ± 0.024
1.192ProTyr: 1.192 ± 0.023
0.0ProXaa: 0.0 ± 0.0
Gln
4.662GlnAla: 4.662 ± 0.062
0.152GlnCys: 0.152 ± 0.01
1.494GlnAsp: 1.494 ± 0.026
1.388GlnGlu: 1.388 ± 0.034
0.794GlnPhe: 0.794 ± 0.024
2.601GlnGly: 2.601 ± 0.046
0.51GlnHis: 0.51 ± 0.018
1.406GlnIle: 1.406 ± 0.032
0.737GlnLys: 0.737 ± 0.021
2.215GlnLeu: 2.215 ± 0.037
0.583GlnMet: 0.583 ± 0.017
0.639GlnAsn: 0.639 ± 0.021
1.581GlnPro: 1.581 ± 0.032
0.891GlnGln: 0.891 ± 0.029
2.209GlnArg: 2.209 ± 0.037
1.33GlnSer: 1.33 ± 0.029
1.377GlnThr: 1.377 ± 0.03
2.348GlnVal: 2.348 ± 0.04
0.287GlnTrp: 0.287 ± 0.012
0.514GlnTyr: 0.514 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
10.734ArgAla: 10.734 ± 0.097
0.568ArgCys: 0.568 ± 0.016
4.946ArgAsp: 4.946 ± 0.062
4.728ArgGlu: 4.728 ± 0.055
3.041ArgPhe: 3.041 ± 0.038
5.933ArgGly: 5.933 ± 0.07
2.027ArgHis: 2.027 ± 0.037
4.068ArgIle: 4.068 ± 0.056
1.694ArgLys: 1.694 ± 0.037
10.022ArgLeu: 10.022 ± 0.095
1.824ArgMet: 1.824 ± 0.035
1.623ArgAsn: 1.623 ± 0.031
4.95ArgPro: 4.95 ± 0.057
2.617ArgGln: 2.617 ± 0.047
7.712ArgArg: 7.712 ± 0.078
3.825ArgSer: 3.825 ± 0.05
4.018ArgThr: 4.018 ± 0.049
5.776ArgVal: 5.776 ± 0.053
1.021ArgTrp: 1.021 ± 0.026
1.69ArgTyr: 1.69 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
5.978SerAla: 5.978 ± 0.066
0.38SerCys: 0.38 ± 0.017
2.475SerAsp: 2.475 ± 0.04
2.298SerGlu: 2.298 ± 0.036
1.784SerPhe: 1.784 ± 0.032
5.258SerGly: 5.258 ± 0.067
0.898SerHis: 0.898 ± 0.025
1.86SerIle: 1.86 ± 0.043
0.994SerLys: 0.994 ± 0.027
5.604SerLeu: 5.604 ± 0.066
0.879SerMet: 0.879 ± 0.022
0.934SerAsn: 0.934 ± 0.025
2.944SerPro: 2.944 ± 0.05
1.321SerGln: 1.321 ± 0.033
3.686SerArg: 3.686 ± 0.055
2.207SerSer: 2.207 ± 0.043
2.232SerThr: 2.232 ± 0.038
3.563SerVal: 3.563 ± 0.054
0.642SerTrp: 0.642 ± 0.021
1.103SerTyr: 1.103 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
6.682ThrAla: 6.682 ± 0.077
0.422ThrCys: 0.422 ± 0.017
2.663ThrAsp: 2.663 ± 0.045
2.477ThrGlu: 2.477 ± 0.038
1.86ThrPhe: 1.86 ± 0.037
5.601ThrGly: 5.601 ± 0.066
0.963ThrHis: 0.963 ± 0.026
2.483ThrIle: 2.483 ± 0.042
1.126ThrLys: 1.126 ± 0.029
6.245ThrLeu: 6.245 ± 0.055
1.011ThrMet: 1.011 ± 0.025
1.028ThrAsn: 1.028 ± 0.026
3.364ThrPro: 3.364 ± 0.045
1.291ThrGln: 1.291 ± 0.028
3.78ThrArg: 3.78 ± 0.049
2.336ThrSer: 2.336 ± 0.038
2.755ThrThr: 2.755 ± 0.051
4.753ThrVal: 4.753 ± 0.049
0.686ThrTrp: 0.686 ± 0.017
1.183ThrTyr: 1.183 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
11.347ValAla: 11.347 ± 0.086
0.599ValCys: 0.599 ± 0.019
3.781ValAsp: 3.781 ± 0.049
4.606ValGlu: 4.606 ± 0.058
2.506ValPhe: 2.506 ± 0.042
6.132ValGly: 6.132 ± 0.06
1.339ValHis: 1.339 ± 0.029
3.39ValIle: 3.39 ± 0.048
1.75ValLys: 1.75 ± 0.036
8.217ValLeu: 8.217 ± 0.067
1.704ValMet: 1.704 ± 0.033
1.616ValAsn: 1.616 ± 0.033
4.625ValPro: 4.625 ± 0.05
2.127ValGln: 2.127 ± 0.038
5.864ValArg: 5.864 ± 0.067
4.008ValSer: 4.008 ± 0.045
4.932ValThr: 4.932 ± 0.058
6.819ValVal: 6.819 ± 0.088
0.868ValTrp: 0.868 ± 0.024
1.369ValTyr: 1.369 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.243TrpAla: 1.243 ± 0.029
0.145TrpCys: 0.145 ± 0.009
0.622TrpAsp: 0.622 ± 0.02
0.502TrpGlu: 0.502 ± 0.017
0.469TrpPhe: 0.469 ± 0.017
0.897TrpGly: 0.897 ± 0.024
0.329TrpHis: 0.329 ± 0.015
0.568TrpIle: 0.568 ± 0.019
0.301TrpLys: 0.301 ± 0.014
1.595TrpLeu: 1.595 ± 0.034
0.267TrpMet: 0.267 ± 0.012
0.359TrpAsn: 0.359 ± 0.015
0.728TrpPro: 0.728 ± 0.02
0.476TrpGln: 0.476 ± 0.018
1.345TrpArg: 1.345 ± 0.027
0.771TrpSer: 0.771 ± 0.021
0.785TrpThr: 0.785 ± 0.023
0.705TrpVal: 0.705 ± 0.023
0.215TrpTrp: 0.215 ± 0.011
0.265TrpTyr: 0.265 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.78TyrAla: 2.78 ± 0.037
0.213TyrCys: 0.213 ± 0.012
1.3TyrAsp: 1.3 ± 0.033
1.058TyrGlu: 1.058 ± 0.027
0.709TyrPhe: 0.709 ± 0.019
2.234TyrGly: 2.234 ± 0.047
0.408TyrHis: 0.408 ± 0.015
0.606TyrIle: 0.606 ± 0.022
0.423TyrLys: 0.423 ± 0.017
2.178TyrLeu: 2.178 ± 0.037
0.308TyrMet: 0.308 ± 0.012
0.425TyrAsn: 0.425 ± 0.019
1.068TyrPro: 1.068 ± 0.027
0.54TyrGln: 0.54 ± 0.018
1.888TyrArg: 1.888 ± 0.031
0.817TyrSer: 0.817 ± 0.021
0.977TyrThr: 0.977 ± 0.024
1.448TyrVal: 1.448 ± 0.031
0.321TyrTrp: 0.321 ± 0.015
0.492TyrTyr: 0.492 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5746 proteins (1734764 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski