Amino acid dipepetide frequency for Staphylococcus virus IPLA35

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.808AlaAla: 2.808 ± 0.75
0.36AlaCys: 0.36 ± 0.138
2.448AlaAsp: 2.448 ± 0.402
4.176AlaGlu: 4.176 ± 0.446
1.872AlaPhe: 1.872 ± 0.357
3.672AlaGly: 3.672 ± 0.745
0.864AlaHis: 0.864 ± 0.28
4.608AlaIle: 4.608 ± 0.67
6.552AlaLys: 6.552 ± 1.071
5.184AlaLeu: 5.184 ± 0.646
1.296AlaMet: 1.296 ± 0.273
4.248AlaAsn: 4.248 ± 0.781
1.512AlaPro: 1.512 ± 0.376
1.656AlaGln: 1.656 ± 0.413
2.592AlaArg: 2.592 ± 0.353
4.824AlaSer: 4.824 ± 0.76
3.528AlaThr: 3.528 ± 0.566
2.664AlaVal: 2.664 ± 0.427
1.296AlaTrp: 1.296 ± 0.348
2.952AlaTyr: 2.952 ± 0.549
0.0AlaXaa: 0.0 ± 0.0
Cys
0.144CysAla: 0.144 ± 0.095
0.072CysCys: 0.072 ± 0.087
0.288CysAsp: 0.288 ± 0.146
0.36CysGlu: 0.36 ± 0.208
0.36CysPhe: 0.36 ± 0.151
0.288CysGly: 0.288 ± 0.162
0.144CysHis: 0.144 ± 0.121
0.36CysIle: 0.36 ± 0.152
0.648CysLys: 0.648 ± 0.205
0.504CysLeu: 0.504 ± 0.217
0.144CysMet: 0.144 ± 0.116
0.144CysAsn: 0.144 ± 0.103
0.072CysPro: 0.072 ± 0.069
0.216CysGln: 0.216 ± 0.132
0.288CysArg: 0.288 ± 0.159
0.144CysSer: 0.144 ± 0.111
0.216CysThr: 0.216 ± 0.112
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.36CysTyr: 0.36 ± 0.177
0.0CysXaa: 0.0 ± 0.0
Asp
2.664AspAla: 2.664 ± 0.539
0.072AspCys: 0.072 ± 0.085
3.24AspAsp: 3.24 ± 0.659
4.896AspGlu: 4.896 ± 0.669
3.384AspPhe: 3.384 ± 0.453
3.24AspGly: 3.24 ± 0.529
0.936AspHis: 0.936 ± 0.237
5.256AspIle: 5.256 ± 0.528
6.336AspLys: 6.336 ± 0.601
5.4AspLeu: 5.4 ± 0.504
2.016AspMet: 2.016 ± 0.349
2.952AspAsn: 2.952 ± 0.466
1.008AspPro: 1.008 ± 0.3
1.584AspGln: 1.584 ± 0.312
2.448AspArg: 2.448 ± 0.439
3.456AspSer: 3.456 ± 0.539
3.816AspThr: 3.816 ± 0.41
3.96AspVal: 3.96 ± 0.455
0.936AspTrp: 0.936 ± 0.212
3.24AspTyr: 3.24 ± 0.592
0.0AspXaa: 0.0 ± 0.0
Glu
5.112GluAla: 5.112 ± 0.537
0.36GluCys: 0.36 ± 0.176
4.752GluAsp: 4.752 ± 0.77
7.56GluGlu: 7.56 ± 1.215
2.808GluPhe: 2.808 ± 0.419
3.312GluGly: 3.312 ± 0.588
1.08GluHis: 1.08 ± 0.274
5.472GluIle: 5.472 ± 0.814
8.425GluLys: 8.425 ± 0.922
7.776GluLeu: 7.776 ± 0.825
2.232GluMet: 2.232 ± 0.43
5.04GluAsn: 5.04 ± 0.628
1.44GluPro: 1.44 ± 0.336
3.024GluGln: 3.024 ± 0.53
3.168GluArg: 3.168 ± 0.561
3.672GluSer: 3.672 ± 0.503
4.248GluThr: 4.248 ± 0.606
4.032GluVal: 4.032 ± 0.472
1.008GluTrp: 1.008 ± 0.217
2.88GluTyr: 2.88 ± 0.529
0.0GluXaa: 0.0 ± 0.0
Phe
1.872PheAla: 1.872 ± 0.358
0.576PheCys: 0.576 ± 0.201
2.952PheAsp: 2.952 ± 0.423
3.168PheGlu: 3.168 ± 0.474
1.08PhePhe: 1.08 ± 0.237
3.384PheGly: 3.384 ± 0.531
0.36PheHis: 0.36 ± 0.132
3.24PheIle: 3.24 ± 0.577
4.104PheLys: 4.104 ± 0.555
2.448PheLeu: 2.448 ± 0.375
1.152PheMet: 1.152 ± 0.272
3.24PheAsn: 3.24 ± 0.493
1.08PhePro: 1.08 ± 0.348
1.152PheGln: 1.152 ± 0.274
1.152PheArg: 1.152 ± 0.255
2.16PheSer: 2.16 ± 0.374
2.232PheThr: 2.232 ± 0.439
1.944PheVal: 1.944 ± 0.403
0.288PheTrp: 0.288 ± 0.128
1.872PheTyr: 1.872 ± 0.383
0.0PheXaa: 0.0 ± 0.0
Gly
4.032GlyAla: 4.032 ± 0.908
0.216GlyCys: 0.216 ± 0.119
3.816GlyAsp: 3.816 ± 0.444
3.744GlyGlu: 3.744 ± 0.461
2.664GlyPhe: 2.664 ± 0.392
5.112GlyGly: 5.112 ± 1.095
1.296GlyHis: 1.296 ± 0.344
3.744GlyIle: 3.744 ± 0.515
6.192GlyLys: 6.192 ± 0.636
5.4GlyLeu: 5.4 ± 0.968
1.512GlyMet: 1.512 ± 0.375
3.024GlyAsn: 3.024 ± 0.595
0.864GlyPro: 0.864 ± 0.228
1.728GlyGln: 1.728 ± 0.407
2.016GlyArg: 2.016 ± 0.496
3.456GlySer: 3.456 ± 0.543
3.672GlyThr: 3.672 ± 0.565
4.104GlyVal: 4.104 ± 0.709
0.864GlyTrp: 0.864 ± 0.24
2.16GlyTyr: 2.16 ± 0.452
0.0GlyXaa: 0.0 ± 0.0
His
0.936HisAla: 0.936 ± 0.246
0.144HisCys: 0.144 ± 0.1
0.576HisAsp: 0.576 ± 0.26
1.152HisGlu: 1.152 ± 0.226
0.648HisPhe: 0.648 ± 0.183
0.936HisGly: 0.936 ± 0.256
0.432HisHis: 0.432 ± 0.179
1.296HisIle: 1.296 ± 0.339
1.44HisLys: 1.44 ± 0.334
1.728HisLeu: 1.728 ± 0.345
0.36HisMet: 0.36 ± 0.14
0.936HisAsn: 0.936 ± 0.26
0.792HisPro: 0.792 ± 0.169
0.576HisGln: 0.576 ± 0.175
0.792HisArg: 0.792 ± 0.221
1.224HisSer: 1.224 ± 0.243
1.08HisThr: 1.08 ± 0.335
0.72HisVal: 0.72 ± 0.243
0.288HisTrp: 0.288 ± 0.144
1.296HisTyr: 1.296 ± 0.251
0.0HisXaa: 0.0 ± 0.0
Ile
4.104IleAla: 4.104 ± 0.636
0.288IleCys: 0.288 ± 0.19
5.184IleAsp: 5.184 ± 0.685
6.264IleGlu: 6.264 ± 0.642
2.52IlePhe: 2.52 ± 0.569
3.096IleGly: 3.096 ± 0.518
1.296IleHis: 1.296 ± 0.299
4.464IleIle: 4.464 ± 0.765
7.776IleLys: 7.776 ± 0.838
5.112IleLeu: 5.112 ± 0.819
1.872IleMet: 1.872 ± 0.352
5.4IleAsn: 5.4 ± 0.666
2.376IlePro: 2.376 ± 0.369
1.944IleGln: 1.944 ± 0.355
3.528IleArg: 3.528 ± 0.457
4.32IleSer: 4.32 ± 0.441
4.248IleThr: 4.248 ± 0.616
3.672IleVal: 3.672 ± 0.572
0.36IleTrp: 0.36 ± 0.129
2.592IleTyr: 2.592 ± 0.533
0.0IleXaa: 0.0 ± 0.0
Lys
7.416LysAla: 7.416 ± 1.102
0.288LysCys: 0.288 ± 0.123
5.544LysAsp: 5.544 ± 0.55
8.641LysGlu: 8.641 ± 1.017
2.16LysPhe: 2.16 ± 0.349
5.472LysGly: 5.472 ± 0.802
2.16LysHis: 2.16 ± 0.401
5.904LysIle: 5.904 ± 0.632
8.281LysLys: 8.281 ± 0.994
9.145LysLeu: 9.145 ± 0.943
2.952LysMet: 2.952 ± 0.417
5.76LysAsn: 5.76 ± 0.738
2.304LysPro: 2.304 ± 0.38
5.184LysGln: 5.184 ± 0.516
3.528LysArg: 3.528 ± 0.636
6.048LysSer: 6.048 ± 1.134
4.968LysThr: 4.968 ± 0.677
6.048LysVal: 6.048 ± 0.792
2.016LysTrp: 2.016 ± 0.402
5.04LysTyr: 5.04 ± 0.638
0.0LysXaa: 0.0 ± 0.0
Leu
5.04LeuAla: 5.04 ± 0.704
0.432LeuCys: 0.432 ± 0.188
5.688LeuAsp: 5.688 ± 0.859
6.408LeuGlu: 6.408 ± 0.64
3.24LeuPhe: 3.24 ± 0.462
4.248LeuGly: 4.248 ± 0.83
1.296LeuHis: 1.296 ± 0.382
5.4LeuIle: 5.4 ± 0.663
8.497LeuLys: 8.497 ± 1.14
6.768LeuLeu: 6.768 ± 0.81
2.016LeuMet: 2.016 ± 0.361
5.328LeuAsn: 5.328 ± 0.563
3.096LeuPro: 3.096 ± 0.518
3.024LeuGln: 3.024 ± 0.56
3.6LeuArg: 3.6 ± 0.432
6.48LeuSer: 6.48 ± 0.791
5.112LeuThr: 5.112 ± 0.675
4.32LeuVal: 4.32 ± 0.535
0.432LeuTrp: 0.432 ± 0.19
3.312LeuTyr: 3.312 ± 0.695
0.0LeuXaa: 0.0 ± 0.0
Met
1.152MetAla: 1.152 ± 0.26
0.144MetCys: 0.144 ± 0.102
1.152MetAsp: 1.152 ± 0.299
1.728MetGlu: 1.728 ± 0.438
1.08MetPhe: 1.08 ± 0.295
1.44MetGly: 1.44 ± 0.5
0.504MetHis: 0.504 ± 0.19
1.368MetIle: 1.368 ± 0.27
2.808MetLys: 2.808 ± 0.524
1.872MetLeu: 1.872 ± 0.469
0.504MetMet: 0.504 ± 0.163
1.656MetAsn: 1.656 ± 0.362
0.864MetPro: 0.864 ± 0.208
1.8MetGln: 1.8 ± 0.389
1.008MetArg: 1.008 ± 0.224
2.016MetSer: 2.016 ± 0.365
2.16MetThr: 2.16 ± 0.325
1.08MetVal: 1.08 ± 0.213
0.288MetTrp: 0.288 ± 0.131
0.936MetTyr: 0.936 ± 0.219
0.0MetXaa: 0.0 ± 0.0
Asn
3.744AsnAla: 3.744 ± 0.494
0.288AsnCys: 0.288 ± 0.184
4.248AsnAsp: 4.248 ± 0.636
3.888AsnGlu: 3.888 ± 0.603
1.512AsnPhe: 1.512 ± 0.272
4.608AsnGly: 4.608 ± 0.545
1.008AsnHis: 1.008 ± 0.314
4.32AsnIle: 4.32 ± 0.502
7.272AsnLys: 7.272 ± 0.719
4.752AsnLeu: 4.752 ± 0.506
0.864AsnMet: 0.864 ± 0.21
4.176AsnAsn: 4.176 ± 0.574
2.304AsnPro: 2.304 ± 0.328
2.808AsnGln: 2.808 ± 0.415
2.952AsnArg: 2.952 ± 0.511
4.104AsnSer: 4.104 ± 0.644
3.744AsnThr: 3.744 ± 0.408
3.168AsnVal: 3.168 ± 0.581
1.224AsnTrp: 1.224 ± 0.343
2.664AsnTyr: 2.664 ± 0.489
0.0AsnXaa: 0.0 ± 0.0
Pro
1.152ProAla: 1.152 ± 0.249
0.216ProCys: 0.216 ± 0.13
1.368ProAsp: 1.368 ± 0.332
2.376ProGlu: 2.376 ± 0.518
1.512ProPhe: 1.512 ± 0.381
1.728ProGly: 1.728 ± 0.348
0.288ProHis: 0.288 ± 0.139
1.728ProIle: 1.728 ± 0.326
2.304ProLys: 2.304 ± 0.516
2.232ProLeu: 2.232 ± 0.474
0.648ProMet: 0.648 ± 0.226
2.016ProAsn: 2.016 ± 0.335
0.648ProPro: 0.648 ± 0.234
1.152ProGln: 1.152 ± 0.279
1.296ProArg: 1.296 ± 0.258
2.448ProSer: 2.448 ± 0.322
1.512ProThr: 1.512 ± 0.332
1.08ProVal: 1.08 ± 0.332
0.36ProTrp: 0.36 ± 0.153
1.08ProTyr: 1.08 ± 0.274
0.0ProXaa: 0.0 ± 0.0
Gln
2.808GlnAla: 2.808 ± 0.373
0.288GlnCys: 0.288 ± 0.158
2.088GlnAsp: 2.088 ± 0.382
2.376GlnGlu: 2.376 ± 0.442
1.44GlnPhe: 1.44 ± 0.313
2.16GlnGly: 2.16 ± 0.409
0.72GlnHis: 0.72 ± 0.198
3.096GlnIle: 3.096 ± 0.59
3.24GlnLys: 3.24 ± 0.361
3.744GlnLeu: 3.744 ± 0.546
0.864GlnMet: 0.864 ± 0.299
1.944GlnAsn: 1.944 ± 0.436
1.08GlnPro: 1.08 ± 0.242
1.296GlnGln: 1.296 ± 0.419
2.088GlnArg: 2.088 ± 0.457
2.52GlnSer: 2.52 ± 0.412
1.296GlnThr: 1.296 ± 0.357
1.872GlnVal: 1.872 ± 0.408
0.504GlnTrp: 0.504 ± 0.174
1.8GlnTyr: 1.8 ± 0.342
0.0GlnXaa: 0.0 ± 0.0
Arg
2.664ArgAla: 2.664 ± 0.479
0.0ArgCys: 0.0 ± 0.0
3.096ArgAsp: 3.096 ± 0.37
2.52ArgGlu: 2.52 ± 0.445
2.088ArgPhe: 2.088 ± 0.354
2.088ArgGly: 2.088 ± 0.391
0.792ArgHis: 0.792 ± 0.216
3.528ArgIle: 3.528 ± 0.497
4.536ArgLys: 4.536 ± 0.51
3.6ArgLeu: 3.6 ± 0.603
1.008ArgMet: 1.008 ± 0.276
2.736ArgAsn: 2.736 ± 0.467
0.72ArgPro: 0.72 ± 0.256
1.152ArgGln: 1.152 ± 0.283
1.8ArgArg: 1.8 ± 0.357
1.584ArgSer: 1.584 ± 0.339
2.448ArgThr: 2.448 ± 0.425
2.232ArgVal: 2.232 ± 0.407
0.216ArgTrp: 0.216 ± 0.117
2.448ArgTyr: 2.448 ± 0.505
0.0ArgXaa: 0.0 ± 0.0
Ser
3.672SerAla: 3.672 ± 0.778
0.288SerCys: 0.288 ± 0.155
4.608SerAsp: 4.608 ± 0.586
4.392SerGlu: 4.392 ± 0.547
2.592SerPhe: 2.592 ± 0.535
4.32SerGly: 4.32 ± 0.866
0.72SerHis: 0.72 ± 0.207
4.032SerIle: 4.032 ± 0.533
6.12SerLys: 6.12 ± 1.048
4.752SerLeu: 4.752 ± 0.588
2.232SerMet: 2.232 ± 0.355
4.752SerAsn: 4.752 ± 0.538
1.8SerPro: 1.8 ± 0.402
2.664SerGln: 2.664 ± 0.431
2.304SerArg: 2.304 ± 0.376
3.672SerSer: 3.672 ± 0.585
3.24SerThr: 3.24 ± 0.483
4.248SerVal: 4.248 ± 0.575
0.936SerTrp: 0.936 ± 0.209
2.304SerTyr: 2.304 ± 0.382
0.0SerXaa: 0.0 ± 0.0
Thr
3.816ThrAla: 3.816 ± 0.545
0.072ThrCys: 0.072 ± 0.071
3.384ThrAsp: 3.384 ± 0.505
4.392ThrGlu: 4.392 ± 0.559
2.808ThrPhe: 2.808 ± 0.341
4.176ThrGly: 4.176 ± 0.55
1.512ThrHis: 1.512 ± 0.309
4.752ThrIle: 4.752 ± 0.625
5.328ThrLys: 5.328 ± 0.728
4.536ThrLeu: 4.536 ± 0.645
0.792ThrMet: 0.792 ± 0.243
3.6ThrAsn: 3.6 ± 0.519
2.088ThrPro: 2.088 ± 0.29
1.656ThrGln: 1.656 ± 0.274
1.656ThrArg: 1.656 ± 0.327
3.096ThrSer: 3.096 ± 0.419
3.024ThrThr: 3.024 ± 0.59
4.248ThrVal: 4.248 ± 0.502
0.432ThrTrp: 0.432 ± 0.205
2.16ThrTyr: 2.16 ± 0.444
0.0ThrXaa: 0.0 ± 0.0
Val
2.952ValAla: 2.952 ± 0.456
0.216ValCys: 0.216 ± 0.138
3.384ValAsp: 3.384 ± 0.571
5.616ValGlu: 5.616 ± 0.748
2.304ValPhe: 2.304 ± 0.444
3.312ValGly: 3.312 ± 0.534
0.936ValHis: 0.936 ± 0.215
4.392ValIle: 4.392 ± 0.506
4.464ValLys: 4.464 ± 0.647
4.176ValLeu: 4.176 ± 0.549
1.224ValMet: 1.224 ± 0.264
3.384ValAsn: 3.384 ± 0.47
1.368ValPro: 1.368 ± 0.347
2.232ValGln: 2.232 ± 0.355
2.664ValArg: 2.664 ± 0.447
4.104ValSer: 4.104 ± 0.558
3.6ValThr: 3.6 ± 0.635
3.24ValVal: 3.24 ± 0.522
0.576ValTrp: 0.576 ± 0.184
2.088ValTyr: 2.088 ± 0.396
0.0ValXaa: 0.0 ± 0.0
Trp
0.72TrpAla: 0.72 ± 0.197
0.0TrpCys: 0.0 ± 0.0
0.432TrpAsp: 0.432 ± 0.187
0.864TrpGlu: 0.864 ± 0.261
1.296TrpPhe: 1.296 ± 0.313
0.36TrpGly: 0.36 ± 0.163
0.0TrpHis: 0.0 ± 0.0
0.936TrpIle: 0.936 ± 0.292
0.792TrpLys: 0.792 ± 0.257
1.224TrpLeu: 1.224 ± 0.252
0.36TrpMet: 0.36 ± 0.194
0.936TrpAsn: 0.936 ± 0.257
0.288TrpPro: 0.288 ± 0.214
0.432TrpGln: 0.432 ± 0.139
0.576TrpArg: 0.576 ± 0.202
1.152TrpSer: 1.152 ± 0.365
0.648TrpThr: 0.648 ± 0.2
0.792TrpVal: 0.792 ± 0.214
0.144TrpTrp: 0.144 ± 0.107
0.72TrpTyr: 0.72 ± 0.251
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.376TyrAla: 2.376 ± 0.324
0.432TyrCys: 0.432 ± 0.155
2.592TyrAsp: 2.592 ± 0.577
2.88TyrGlu: 2.88 ± 0.534
2.088TyrPhe: 2.088 ± 0.478
2.664TyrGly: 2.664 ± 0.588
1.008TyrHis: 1.008 ± 0.323
2.52TyrIle: 2.52 ± 0.467
3.744TyrLys: 3.744 ± 0.493
3.384TyrLeu: 3.384 ± 0.516
1.44TyrMet: 1.44 ± 0.263
2.376TyrAsn: 2.376 ± 0.489
1.44TyrPro: 1.44 ± 0.321
1.944TyrGln: 1.944 ± 0.315
1.8TyrArg: 1.8 ± 0.477
3.168TyrSer: 3.168 ± 0.444
2.736TyrThr: 2.736 ± 0.554
2.808TyrVal: 2.808 ± 0.532
0.504TyrTrp: 0.504 ± 0.17
1.584TyrTyr: 1.584 ± 0.431
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (13889 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski