Lactococcus phage P1046

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fremauxvirus; unclassified Fremauxvirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649V3M5|A0A649V3M5_9CAUD Uncharacterized protein OS=Lactococcus phage P1046 OX=2662294 PE=4 SV=1
MM1 pKa = 7.77LSTSDD6 pKa = 3.59NPYY9 pKa = 10.84NPWTQYY15 pKa = 11.46DD16 pKa = 3.61KK17 pKa = 11.05WLDD20 pKa = 2.9WDD22 pKa = 3.97TTHH25 pKa = 7.45GYY27 pKa = 7.99NTINYY32 pKa = 7.25VARR35 pKa = 11.84LANTLEE41 pKa = 4.25SMDD44 pKa = 4.02EE45 pKa = 3.94EE46 pKa = 4.46ATLEE50 pKa = 4.27VYY52 pKa = 9.86QAAVDD57 pKa = 5.21AIMEE61 pKa = 4.18QNLTGNYY68 pKa = 9.8VIVPKK73 pKa = 9.38PTDD76 pKa = 3.63YY77 pKa = 11.65VEE79 pKa = 5.48ADD81 pKa = 3.09ASLYY85 pKa = 9.71EE86 pKa = 4.09

Molecular weight: 9.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V1T2|A0A649V1T2_9CAUD Uncharacterized protein OS=Lactococcus phage P1046 OX=2662294 PE=4 SV=1
MM1 pKa = 7.66LIISTYY7 pKa = 8.51IKK9 pKa = 9.88RR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 9.17LVRR15 pKa = 11.84SMIMEE20 pKa = 4.46NWASAVIGAIVAGFTFLTAIATLATKK46 pKa = 10.62RR47 pKa = 11.84NDD49 pKa = 3.11SSSRR53 pKa = 11.84MTNKK57 pKa = 10.08SFDD60 pKa = 4.2IISKK64 pKa = 10.38RR65 pKa = 11.84LDD67 pKa = 2.98IAEE70 pKa = 4.56AKK72 pKa = 10.24VSSLEE77 pKa = 4.23SVQSDD82 pKa = 3.56YY83 pKa = 11.29RR84 pKa = 11.84II85 pKa = 3.49

Molecular weight: 9.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

16863

34

1998

219.0

24.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.365 ± 0.569

0.421 ± 0.077

6.393 ± 0.322

6.09 ± 0.541

3.659 ± 0.236

7.092 ± 0.504

1.571 ± 0.125

7.324 ± 0.262

7.62 ± 0.441

7.893 ± 0.35

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.13

6.66 ± 0.273

3.238 ± 0.176

3.73 ± 0.207

3.309 ± 0.308

6.405 ± 0.319

6.92 ± 0.412

6.304 ± 0.27

1.311 ± 0.129

4.021 ± 0.396
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski