Tortoise microvirus 76

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8WA65|A0A4P8WA65_9VIRU Uncharacterized protein OS=Tortoise microvirus 76 OX=2583183 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84KK3 pKa = 9.29IGNLNLWHH11 pKa = 6.83LVAIDD16 pKa = 3.64NALNLPNPNPRR27 pKa = 11.84RR28 pKa = 11.84VDD30 pKa = 2.98IEE32 pKa = 4.37FNTTSEE38 pKa = 4.34TTIHH42 pKa = 7.36AIQDD46 pKa = 3.69GEE48 pKa = 4.23ATLLGVVDD56 pKa = 4.83GLEE59 pKa = 3.85QFQFDD64 pKa = 3.97TDD66 pKa = 3.61GKK68 pKa = 8.05TEE70 pKa = 3.78IVFYY74 pKa = 11.3VSDD77 pKa = 3.64TDD79 pKa = 3.82AEE81 pKa = 4.1AEE83 pKa = 3.75IWVRR87 pKa = 11.84CQEE90 pKa = 4.22LAFSPLDD97 pKa = 3.81DD98 pKa = 4.59VEE100 pKa = 5.76DD101 pKa = 4.78EE102 pKa = 4.38IPFVNILEE110 pKa = 4.24RR111 pKa = 11.84EE112 pKa = 4.25EE113 pKa = 4.1ISEE116 pKa = 4.13DD117 pKa = 3.63LQIILNKK124 pKa = 9.96VNEE127 pKa = 4.19NMNRR131 pKa = 11.84RR132 pKa = 11.84LATAEE137 pKa = 3.75RR138 pKa = 11.84EE139 pKa = 3.87IEE141 pKa = 3.85RR142 pKa = 11.84RR143 pKa = 11.84ARR145 pKa = 11.84EE146 pKa = 3.75IADD149 pKa = 3.48AAIAAAASQPEE160 pKa = 4.52QPVAPPPPDD169 pKa = 4.84GEE171 pKa = 4.4DD172 pKa = 3.63QGGDD176 pKa = 3.66DD177 pKa = 5.03NGGGTPNAAAA187 pKa = 4.53

Molecular weight: 20.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W7Q7|A0A4P8W7Q7_9VIRU Uncharacterized protein OS=Tortoise microvirus 76 OX=2583183 PE=4 SV=1
MM1 pKa = 7.62ARR3 pKa = 11.84RR4 pKa = 11.84DD5 pKa = 3.54RR6 pKa = 11.84DD7 pKa = 3.33RR8 pKa = 11.84PRR10 pKa = 11.84QPGTGPGSINWGTHH24 pKa = 5.65RR25 pKa = 11.84PPGWSTSTTQPRR37 pKa = 11.84KK38 pKa = 9.23PSSGSQPSGNRR49 pKa = 11.84SGTRR53 pKa = 11.84PSGKK57 pKa = 10.14GPSARR62 pKa = 11.84PAKK65 pKa = 9.57GQNPSTRR72 pKa = 11.84DD73 pKa = 2.8VWMSGGTTGVRR84 pKa = 11.84SDD86 pKa = 3.89PSRR89 pKa = 11.84GNHH92 pKa = 6.52GYY94 pKa = 10.75NDD96 pKa = 3.89DD97 pKa = 4.0KK98 pKa = 11.53FGGSGGNDD106 pKa = 3.13RR107 pKa = 11.84NHH109 pKa = 5.95STGGSPGRR117 pKa = 11.84GGVPARR123 pKa = 11.84TNNRR127 pKa = 11.84WSGVADD133 pKa = 3.76PVRR136 pKa = 11.84NGTGLGSEE144 pKa = 4.26TRR146 pKa = 11.84PGGGPTGPGVPGFQRR161 pKa = 11.84KK162 pKa = 7.6PHH164 pKa = 6.07GKK166 pKa = 9.04PGRR169 pKa = 11.84KK170 pKa = 8.85PYY172 pKa = 10.36QDD174 pKa = 3.88PNYY177 pKa = 9.8VGYY180 pKa = 10.52DD181 pKa = 3.46GTVGNPRR188 pKa = 11.84SNLPTPFEE196 pKa = 4.14GRR198 pKa = 11.84TPYY201 pKa = 10.41QDD203 pKa = 3.76PNYY206 pKa = 10.26KK207 pKa = 10.55GYY209 pKa = 10.82DD210 pKa = 3.43VTKK213 pKa = 10.28GKK215 pKa = 9.49PGKK218 pKa = 9.92KK219 pKa = 9.24EE220 pKa = 4.02YY221 pKa = 9.9KK222 pKa = 9.84CKK224 pKa = 9.99PRR226 pKa = 11.84PEE228 pKa = 4.84DD229 pKa = 3.28NSKK232 pKa = 10.61VEE234 pKa = 4.93DD235 pKa = 3.94EE236 pKa = 5.08DD237 pKa = 4.71RR238 pKa = 11.84DD239 pKa = 3.77PSGGGGGKK247 pKa = 9.23IAGPKK252 pKa = 9.58KK253 pKa = 10.66DD254 pKa = 3.87FVPWCSS260 pKa = 2.97

Molecular weight: 27.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2014

82

534

287.7

31.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.235 ± 1.095

0.546 ± 0.235

5.809 ± 0.46

5.511 ± 1.167

3.178 ± 0.375

10.328 ± 1.943

1.639 ± 0.323

3.873 ± 0.726

5.809 ± 0.841

6.455 ± 1.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.085 ± 0.47

4.866 ± 0.598

6.753 ± 1.148

4.667 ± 0.444

7.001 ± 0.847

5.362 ± 0.8

5.263 ± 0.482

6.753 ± 0.537

2.284 ± 0.407

2.582 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski