Pichia membranifaciens NRRL Y-2026

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Pichiaceae; Pichia; Pichia membranifaciens

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5536 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3NQ67|A0A1E3NQ67_9ASCO Uncharacterized protein OS=Pichia membranifaciens NRRL Y-2026 OX=763406 GN=PICMEDRAFT_125338 PE=4 SV=1
MM1 pKa = 6.97MSSYY5 pKa = 10.36FQSTYY10 pKa = 10.07NSGRR14 pKa = 11.84IAQTYY19 pKa = 10.23LLASKK24 pKa = 10.46VKK26 pKa = 10.81AKK28 pKa = 8.93LTQEE32 pKa = 3.95AVRR35 pKa = 11.84NDD37 pKa = 3.29VDD39 pKa = 3.6LHH41 pKa = 7.25RR42 pKa = 11.84LVCQANLLDD51 pKa = 4.82NLIEE55 pKa = 4.25NLNHH59 pKa = 6.78HH60 pKa = 6.23EE61 pKa = 4.06SSYY64 pKa = 11.62NSNTSSNPGKK74 pKa = 9.42TNVCYY79 pKa = 10.62DD80 pKa = 4.3SINSNSYY87 pKa = 9.8PLLNNVTVQVNDD99 pKa = 2.5VDD101 pKa = 4.22YY102 pKa = 11.48VVDD105 pKa = 3.98GDD107 pKa = 4.05ADD109 pKa = 3.7SGEE112 pKa = 4.28EE113 pKa = 4.14DD114 pKa = 4.92DD115 pKa = 5.88EE116 pKa = 4.84VDD118 pKa = 3.43EE119 pKa = 4.71NEE121 pKa = 4.29IKK123 pKa = 10.23IEE125 pKa = 3.94RR126 pKa = 11.84APCSDD131 pKa = 3.3VYY133 pKa = 10.22YY134 pKa = 10.53TSDD137 pKa = 4.79DD138 pKa = 3.7SDD140 pKa = 3.98FDD142 pKa = 4.97SDD144 pKa = 4.91DD145 pKa = 5.19DD146 pKa = 4.68SDD148 pKa = 4.64SSFNSDD154 pKa = 2.92TFGGKK159 pKa = 9.8SSDD162 pKa = 3.9PNEE165 pKa = 4.3LCCIALQRR173 pKa = 11.84LNLHH177 pKa = 6.38GGVEE181 pKa = 4.34DD182 pKa = 5.51GLGEE186 pKa = 5.6DD187 pKa = 5.88DD188 pKa = 6.6DD189 pKa = 5.8DD190 pKa = 6.23LEE192 pKa = 4.43NQATCSSVTATITLSGSDD210 pKa = 3.97SDD212 pKa = 4.72SEE214 pKa = 4.91SNSEE218 pKa = 4.18TEE220 pKa = 4.56SDD222 pKa = 3.27TEE224 pKa = 4.17YY225 pKa = 11.19EE226 pKa = 4.22NEE228 pKa = 4.07NEE230 pKa = 3.98NDD232 pKa = 3.78SRR234 pKa = 11.84DD235 pKa = 3.53HH236 pKa = 7.32GSFSNNYY243 pKa = 7.52CALMRR248 pKa = 11.84MHH250 pKa = 6.52SQHH253 pKa = 6.31TSLNLNDD260 pKa = 3.78YY261 pKa = 9.46VASLEE266 pKa = 4.07ADD268 pKa = 3.48TSVEE272 pKa = 3.98EE273 pKa = 4.43TDD275 pKa = 3.0ACTNIPHH282 pKa = 6.59NSSLDD287 pKa = 3.4EE288 pKa = 4.0MTRR291 pKa = 11.84EE292 pKa = 3.8EE293 pKa = 4.84DD294 pKa = 4.46LPSLSNCSSFSSMEE308 pKa = 4.25EE309 pKa = 3.2IGQPNQNIVRR319 pKa = 11.84LDD321 pKa = 3.55CLDD324 pKa = 4.36SIKK327 pKa = 10.45HH328 pKa = 4.63QHH330 pKa = 6.25DD331 pKa = 3.49TDD333 pKa = 4.06SPSLYY338 pKa = 9.2LTQSQDD344 pKa = 2.98VFLLL348 pKa = 3.98

Molecular weight: 38.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3NHL2|A0A1E3NHL2_9ASCO Cysteinyl-tRNA synthetase OS=Pichia membranifaciens NRRL Y-2026 OX=763406 GN=PICMEDRAFT_16930 PE=3 SV=1
MM1 pKa = 7.93PSQKK5 pKa = 8.79TFRR8 pKa = 11.84VKK10 pKa = 10.56QKK12 pKa = 10.19LAKK15 pKa = 9.92AQNQNRR21 pKa = 11.84PLPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TNNKK34 pKa = 8.41IRR36 pKa = 11.84YY37 pKa = 5.78NAKK40 pKa = 8.42RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.6WKK45 pKa = 8.07RR46 pKa = 11.84TKK48 pKa = 10.68LGLL51 pKa = 3.71

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5536

0

5536

2673506

49

4344

482.9

54.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.108 ± 0.037

1.284 ± 0.011

6.113 ± 0.028

6.572 ± 0.032

4.432 ± 0.024

5.463 ± 0.033

2.105 ± 0.014

6.15 ± 0.028

7.043 ± 0.03

9.533 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.111 ± 0.012

5.69 ± 0.03

4.282 ± 0.021

3.733 ± 0.029

4.408 ± 0.025

8.957 ± 0.044

5.555 ± 0.025

6.003 ± 0.024

1.017 ± 0.01

3.441 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski