Nitrosospira sp. Nsp11

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosospira; unclassified Nitrosospira

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3073 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M7G5J5|A0A1M7G5J5_9PROT Putative hydrolase of the HAD superfamily OS=Nitrosospira sp. Nsp11 OX=1855338 GN=SAMN05216428_11434 PE=4 SV=1
MM1 pKa = 7.36NFLVYY6 pKa = 8.45EE7 pKa = 4.39TTTGAIVRR15 pKa = 11.84SGQCQDD21 pKa = 5.26DD22 pKa = 4.02MLDD25 pKa = 3.49SQSRR29 pKa = 11.84SGEE32 pKa = 3.94TAIEE36 pKa = 4.4GEE38 pKa = 4.42ADD40 pKa = 3.81DD41 pKa = 3.77ATQYY45 pKa = 10.83VDD47 pKa = 3.29SGVVSSRR54 pKa = 11.84PTITATWSKK63 pKa = 11.26LEE65 pKa = 4.16VIANGSDD72 pKa = 3.1TTTLGSSLPNPTEE85 pKa = 3.76VFVFVPDD92 pKa = 3.81GAVAPEE98 pKa = 4.02MEE100 pKa = 4.63IVTSGTFSFATPIVGSYY117 pKa = 8.52TINVIPPFPYY127 pKa = 9.87QPHH130 pKa = 4.99TQIITAII137 pKa = 3.78

Molecular weight: 14.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M7BE01|A0A1M7BE01_9PROT DNA-binding response regulator OmpR family contains REC and winged-helix (WHTH) domain OS=Nitrosospira sp. Nsp11 OX=1855338 GN=SAMN05216428_103106 PE=4 SV=1
MM1 pKa = 7.66EE2 pKa = 5.91PGKK5 pKa = 10.58KK6 pKa = 9.78FNPRR10 pKa = 11.84EE11 pKa = 4.09FPDD14 pKa = 3.4KK15 pKa = 10.24PEE17 pKa = 3.51WMRR20 pKa = 11.84GSFTDD25 pKa = 3.05SRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84DD30 pKa = 3.54FLRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SPRR38 pKa = 11.84IRR40 pKa = 11.84LLRR43 pKa = 11.84EE44 pKa = 3.43MRR46 pKa = 11.84GVRR49 pKa = 11.84TGTGFRR55 pKa = 11.84GIPALTNPTVAYY67 pKa = 9.9YY68 pKa = 10.21DD69 pKa = 3.81ALGAPPFGLEE79 pKa = 3.89PGTAGSLQGTPFHH92 pKa = 7.16SGFQRR97 pKa = 11.84TFTTLRR103 pKa = 11.84FLALLFTNSKK113 pKa = 10.81CEE115 pKa = 4.07LSSCTPDD122 pKa = 3.07QFEE125 pKa = 3.95

Molecular weight: 14.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3073

0

3073

908958

26

3472

295.8

32.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.133 ± 0.052

0.98 ± 0.015

5.24 ± 0.031

5.982 ± 0.044

3.903 ± 0.031

7.68 ± 0.041

2.356 ± 0.023

5.941 ± 0.031

4.397 ± 0.039

10.395 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.021

3.495 ± 0.028

4.754 ± 0.032

3.722 ± 0.031

6.303 ± 0.042

6.034 ± 0.038

5.17 ± 0.032

6.891 ± 0.038

1.299 ± 0.019

2.753 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski