Amino acid dipepetide frequency for Nitrosospira sp. Nsp11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.553AlaAla: 11.553 ± 0.142
1.047AlaCys: 1.047 ± 0.034
5.282AlaAsp: 5.282 ± 0.076
6.28AlaGlu: 6.28 ± 0.102
3.522AlaPhe: 3.522 ± 0.069
8.316AlaGly: 8.316 ± 0.117
2.168AlaHis: 2.168 ± 0.05
5.822AlaIle: 5.822 ± 0.09
4.077AlaLys: 4.077 ± 0.086
11.026AlaLeu: 11.026 ± 0.132
2.887AlaMet: 2.887 ± 0.061
3.183AlaAsn: 3.183 ± 0.064
3.813AlaPro: 3.813 ± 0.067
4.029AlaGln: 4.029 ± 0.08
6.43AlaArg: 6.43 ± 0.099
5.965AlaSer: 5.965 ± 0.082
4.98AlaThr: 4.98 ± 0.073
7.108AlaVal: 7.108 ± 0.086
1.322AlaTrp: 1.322 ± 0.044
2.519AlaTyr: 2.519 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
0.921CysAla: 0.921 ± 0.033
0.152CysCys: 0.152 ± 0.012
0.556CysAsp: 0.556 ± 0.028
0.6CysGlu: 0.6 ± 0.033
0.389CysPhe: 0.389 ± 0.021
0.929CysGly: 0.929 ± 0.034
0.373CysHis: 0.373 ± 0.025
0.534CysIle: 0.534 ± 0.024
0.367CysLys: 0.367 ± 0.021
0.868CysLeu: 0.868 ± 0.033
0.242CysMet: 0.242 ± 0.015
0.35CysAsn: 0.35 ± 0.021
0.495CysPro: 0.495 ± 0.022
0.277CysGln: 0.277 ± 0.018
0.659CysArg: 0.659 ± 0.031
0.564CysSer: 0.564 ± 0.027
0.465CysThr: 0.465 ± 0.026
0.642CysVal: 0.642 ± 0.027
0.12CysTrp: 0.12 ± 0.01
0.293CysTyr: 0.293 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
5.266AspAla: 5.266 ± 0.076
0.493AspCys: 0.493 ± 0.023
2.596AspAsp: 2.596 ± 0.06
3.493AspGlu: 3.493 ± 0.072
2.242AspPhe: 2.242 ± 0.048
3.914AspGly: 3.914 ± 0.083
1.2AspHis: 1.2 ± 0.036
3.344AspIle: 3.344 ± 0.066
2.419AspLys: 2.419 ± 0.056
5.273AspLeu: 5.273 ± 0.085
1.336AspMet: 1.336 ± 0.04
1.758AspAsn: 1.758 ± 0.046
2.574AspPro: 2.574 ± 0.062
1.734AspGln: 1.734 ± 0.047
3.04AspArg: 3.04 ± 0.054
2.928AspSer: 2.928 ± 0.06
2.597AspThr: 2.597 ± 0.062
3.647AspVal: 3.647 ± 0.058
0.8AspTrp: 0.8 ± 0.027
1.742AspTyr: 1.742 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
6.144GluAla: 6.144 ± 0.086
0.483GluCys: 0.483 ± 0.023
2.591GluAsp: 2.591 ± 0.059
3.46GluGlu: 3.46 ± 0.064
2.276GluPhe: 2.276 ± 0.057
3.687GluGly: 3.687 ± 0.07
1.412GluHis: 1.412 ± 0.041
4.203GluIle: 4.203 ± 0.062
3.44GluLys: 3.44 ± 0.068
6.09GluLeu: 6.09 ± 0.086
1.632GluMet: 1.632 ± 0.046
2.34GluAsn: 2.34 ± 0.052
2.412GluPro: 2.412 ± 0.058
2.738GluGln: 2.738 ± 0.064
4.137GluArg: 4.137 ± 0.082
3.308GluSer: 3.308 ± 0.059
3.122GluThr: 3.122 ± 0.061
3.978GluVal: 3.978 ± 0.078
0.805GluTrp: 0.805 ± 0.031
1.56GluTyr: 1.56 ± 0.047
0.0GluXaa: 0.0 ± 0.0
Phe
3.747PheAla: 3.747 ± 0.075
0.468PheCys: 0.468 ± 0.021
2.494PheAsp: 2.494 ± 0.059
2.26PheGlu: 2.26 ± 0.049
1.762PhePhe: 1.762 ± 0.05
3.264PheGly: 3.264 ± 0.06
0.877PheHis: 0.877 ± 0.031
2.274PheIle: 2.274 ± 0.052
1.37PheLys: 1.37 ± 0.039
3.644PheLeu: 3.644 ± 0.078
0.877PheMet: 0.877 ± 0.031
1.472PheAsn: 1.472 ± 0.039
1.753PhePro: 1.753 ± 0.046
1.259PheGln: 1.259 ± 0.037
2.223PheArg: 2.223 ± 0.049
2.816PheSer: 2.816 ± 0.063
2.139PheThr: 2.139 ± 0.045
2.618PheVal: 2.618 ± 0.057
0.55PheTrp: 0.55 ± 0.026
1.162PheTyr: 1.162 ± 0.037
0.0PheXaa: 0.0 ± 0.0
Gly
6.816GlyAla: 6.816 ± 0.092
0.877GlyCys: 0.877 ± 0.035
3.763GlyAsp: 3.763 ± 0.069
4.422GlyGlu: 4.422 ± 0.081
3.247GlyPhe: 3.247 ± 0.064
6.142GlyGly: 6.142 ± 0.114
1.78GlyHis: 1.78 ± 0.052
5.206GlyIle: 5.206 ± 0.081
4.22GlyLys: 4.22 ± 0.071
7.611GlyLeu: 7.611 ± 0.107
2.377GlyMet: 2.377 ± 0.053
2.829GlyAsn: 2.829 ± 0.061
2.462GlyPro: 2.462 ± 0.06
2.59GlyGln: 2.59 ± 0.053
4.593GlyArg: 4.593 ± 0.074
4.677GlySer: 4.677 ± 0.09
4.066GlyThr: 4.066 ± 0.084
5.437GlyVal: 5.437 ± 0.089
1.177GlyTrp: 1.177 ± 0.04
2.509GlyTyr: 2.509 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
2.319HisAla: 2.319 ± 0.054
0.318HisCys: 0.318 ± 0.018
1.25HisAsp: 1.25 ± 0.039
1.405HisGlu: 1.405 ± 0.038
0.947HisPhe: 0.947 ± 0.032
1.954HisGly: 1.954 ± 0.046
0.729HisHis: 0.729 ± 0.032
1.343HisIle: 1.343 ± 0.045
0.825HisLys: 0.825 ± 0.028
2.324HisLeu: 2.324 ± 0.053
0.551HisMet: 0.551 ± 0.025
0.782HisAsn: 0.782 ± 0.033
1.438HisPro: 1.438 ± 0.041
0.882HisGln: 0.882 ± 0.032
1.43HisArg: 1.43 ± 0.041
1.315HisSer: 1.315 ± 0.038
1.1HisThr: 1.1 ± 0.036
1.52HisVal: 1.52 ± 0.042
0.348HisTrp: 0.348 ± 0.022
0.776HisTyr: 0.776 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
6.589IleAla: 6.589 ± 0.09
0.572IleCys: 0.572 ± 0.024
3.463IleAsp: 3.463 ± 0.066
3.998IleGlu: 3.998 ± 0.068
2.219IlePhe: 2.219 ± 0.05
4.731IleGly: 4.731 ± 0.076
1.25IleHis: 1.25 ± 0.037
3.181IleIle: 3.181 ± 0.06
2.67IleLys: 2.67 ± 0.057
5.369IleLeu: 5.369 ± 0.089
1.24IleMet: 1.24 ± 0.035
2.183IleAsn: 2.183 ± 0.054
3.006IlePro: 3.006 ± 0.062
1.854IleGln: 1.854 ± 0.046
3.449IleArg: 3.449 ± 0.06
4.013IleSer: 4.013 ± 0.075
3.362IleThr: 3.362 ± 0.06
4.027IleVal: 4.027 ± 0.071
0.646IleTrp: 0.646 ± 0.029
1.592IleTyr: 1.592 ± 0.039
0.0IleXaa: 0.0 ± 0.0
Lys
4.213LysAla: 4.213 ± 0.082
0.329LysCys: 0.329 ± 0.022
2.15LysAsp: 2.15 ± 0.052
2.585LysGlu: 2.585 ± 0.052
1.391LysPhe: 1.391 ± 0.041
2.859LysGly: 2.859 ± 0.061
0.982LysHis: 0.982 ± 0.036
2.778LysIle: 2.778 ± 0.057
2.393LysLys: 2.393 ± 0.069
4.513LysLeu: 4.513 ± 0.07
1.193LysMet: 1.193 ± 0.037
1.81LysAsn: 1.81 ± 0.049
2.528LysPro: 2.528 ± 0.062
1.894LysGln: 1.894 ± 0.048
2.885LysArg: 2.885 ± 0.056
2.612LysSer: 2.612 ± 0.06
2.524LysThr: 2.524 ± 0.056
2.757LysVal: 2.757 ± 0.067
0.539LysTrp: 0.539 ± 0.025
1.039LysTyr: 1.039 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
11.355LeuAla: 11.355 ± 0.141
1.0LeuCys: 1.0 ± 0.033
5.642LeuAsp: 5.642 ± 0.083
5.843LeuGlu: 5.843 ± 0.091
3.812LeuPhe: 3.812 ± 0.076
7.556LeuGly: 7.556 ± 0.099
2.412LeuHis: 2.412 ± 0.052
5.828LeuIle: 5.828 ± 0.101
4.684LeuLys: 4.684 ± 0.08
11.184LeuLeu: 11.184 ± 0.16
2.591LeuMet: 2.591 ± 0.06
3.507LeuAsn: 3.507 ± 0.062
5.679LeuPro: 5.679 ± 0.099
3.677LeuGln: 3.677 ± 0.065
6.689LeuArg: 6.689 ± 0.1
6.715LeuSer: 6.715 ± 0.107
5.445LeuThr: 5.445 ± 0.078
6.659LeuVal: 6.659 ± 0.098
1.206LeuTrp: 1.206 ± 0.048
2.464LeuTyr: 2.464 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
2.643MetAla: 2.643 ± 0.055
0.172MetCys: 0.172 ± 0.014
1.264MetAsp: 1.264 ± 0.037
1.495MetGlu: 1.495 ± 0.038
0.798MetPhe: 0.798 ± 0.031
1.842MetGly: 1.842 ± 0.043
0.62MetHis: 0.62 ± 0.021
1.384MetIle: 1.384 ± 0.043
1.323MetLys: 1.323 ± 0.039
2.833MetLeu: 2.833 ± 0.066
0.658MetMet: 0.658 ± 0.031
1.037MetAsn: 1.037 ± 0.03
1.446MetPro: 1.446 ± 0.04
1.139MetGln: 1.139 ± 0.039
1.698MetArg: 1.698 ± 0.048
1.676MetSer: 1.676 ± 0.042
1.398MetThr: 1.398 ± 0.035
1.672MetVal: 1.672 ± 0.043
0.215MetTrp: 0.215 ± 0.017
0.422MetTyr: 0.422 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
3.383AsnAla: 3.383 ± 0.058
0.376AsnCys: 0.376 ± 0.022
1.747AsnAsp: 1.747 ± 0.051
1.808AsnGlu: 1.808 ± 0.044
1.475AsnPhe: 1.475 ± 0.042
2.797AsnGly: 2.797 ± 0.059
0.804AsnHis: 0.804 ± 0.029
2.109AsnIle: 2.109 ± 0.052
1.328AsnLys: 1.328 ± 0.04
3.726AsnLeu: 3.726 ± 0.067
0.794AsnMet: 0.794 ± 0.033
1.26AsnAsn: 1.26 ± 0.039
2.159AsnPro: 2.159 ± 0.052
1.262AsnGln: 1.262 ± 0.04
2.234AsnArg: 2.234 ± 0.048
1.951AsnSer: 1.951 ± 0.049
1.83AsnThr: 1.83 ± 0.047
2.33AsnVal: 2.33 ± 0.055
0.512AsnTrp: 0.512 ± 0.021
1.068AsnTyr: 1.068 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
4.891ProAla: 4.891 ± 0.074
0.396ProCys: 0.396 ± 0.021
3.183ProAsp: 3.183 ± 0.07
3.673ProGlu: 3.673 ± 0.069
1.816ProPhe: 1.816 ± 0.043
4.024ProGly: 4.024 ± 0.061
1.1ProHis: 1.1 ± 0.031
2.37ProIle: 2.37 ± 0.051
1.694ProLys: 1.694 ± 0.046
4.676ProLeu: 4.676 ± 0.083
1.112ProMet: 1.112 ± 0.036
1.493ProAsn: 1.493 ± 0.047
2.26ProPro: 2.26 ± 0.066
1.755ProGln: 1.755 ± 0.05
2.391ProArg: 2.391 ± 0.054
2.713ProSer: 2.713 ± 0.057
2.091ProThr: 2.091 ± 0.047
3.976ProVal: 3.976 ± 0.067
0.614ProTrp: 0.614 ± 0.026
1.31ProTyr: 1.31 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
4.083GlnAla: 4.083 ± 0.077
0.308GlnCys: 0.308 ± 0.019
1.582GlnAsp: 1.582 ± 0.045
2.064GlnGlu: 2.064 ± 0.054
1.348GlnPhe: 1.348 ± 0.037
2.503GlnGly: 2.503 ± 0.052
1.004GlnHis: 1.004 ± 0.034
2.225GlnIle: 2.225 ± 0.051
1.694GlnLys: 1.694 ± 0.045
3.892GlnLeu: 3.892 ± 0.07
0.993GlnMet: 0.993 ± 0.03
1.261GlnAsn: 1.261 ± 0.035
1.844GlnPro: 1.844 ± 0.047
1.788GlnGln: 1.788 ± 0.052
2.601GlnArg: 2.601 ± 0.06
2.097GlnSer: 2.097 ± 0.053
1.875GlnThr: 1.875 ± 0.049
2.61GlnVal: 2.61 ± 0.06
0.465GlnTrp: 0.465 ± 0.023
0.984GlnTyr: 0.984 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
5.528ArgAla: 5.528 ± 0.083
0.59ArgCys: 0.59 ± 0.024
3.363ArgAsp: 3.363 ± 0.062
4.05ArgGlu: 4.05 ± 0.082
2.821ArgPhe: 2.821 ± 0.055
4.013ArgGly: 4.013 ± 0.073
1.705ArgHis: 1.705 ± 0.053
4.038ArgIle: 4.038 ± 0.075
2.82ArgLys: 2.82 ± 0.061
6.923ArgLeu: 6.923 ± 0.11
1.784ArgMet: 1.784 ± 0.047
2.46ArgAsn: 2.46 ± 0.049
2.698ArgPro: 2.698 ± 0.057
2.556ArgGln: 2.556 ± 0.058
4.283ArgArg: 4.283 ± 0.101
3.416ArgSer: 3.416 ± 0.062
2.9ArgThr: 2.9 ± 0.057
4.032ArgVal: 4.032 ± 0.068
0.859ArgTrp: 0.859 ± 0.032
2.187ArgTyr: 2.187 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
6.097SerAla: 6.097 ± 0.077
0.596SerCys: 0.596 ± 0.023
2.948SerAsp: 2.948 ± 0.057
3.167SerGlu: 3.167 ± 0.06
2.494SerPhe: 2.494 ± 0.062
5.755SerGly: 5.755 ± 0.109
1.402SerHis: 1.402 ± 0.039
3.449SerIle: 3.449 ± 0.077
2.327SerLys: 2.327 ± 0.057
6.263SerLeu: 6.263 ± 0.087
1.573SerMet: 1.573 ± 0.041
1.94SerAsn: 1.94 ± 0.051
2.871SerPro: 2.871 ± 0.054
2.041SerGln: 2.041 ± 0.046
3.904SerArg: 3.904 ± 0.072
3.874SerSer: 3.874 ± 0.076
3.112SerThr: 3.112 ± 0.065
4.134SerVal: 4.134 ± 0.076
0.794SerTrp: 0.794 ± 0.032
1.594SerTyr: 1.594 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
5.16ThrAla: 5.16 ± 0.081
0.469ThrCys: 0.469 ± 0.026
2.714ThrAsp: 2.714 ± 0.053
2.855ThrGlu: 2.855 ± 0.05
1.966ThrPhe: 1.966 ± 0.051
4.797ThrGly: 4.797 ± 0.079
1.226ThrHis: 1.226 ± 0.039
2.823ThrIle: 2.823 ± 0.061
1.692ThrLys: 1.692 ± 0.045
5.932ThrLeu: 5.932 ± 0.086
1.139ThrMet: 1.139 ± 0.037
1.491ThrAsn: 1.491 ± 0.044
2.91ThrPro: 2.91 ± 0.059
1.76ThrGln: 1.76 ± 0.048
3.127ThrArg: 3.127 ± 0.056
2.995ThrSer: 2.995 ± 0.064
2.735ThrThr: 2.735 ± 0.068
3.909ThrVal: 3.909 ± 0.065
0.591ThrTrp: 0.591 ± 0.026
1.41ThrTyr: 1.41 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
7.032ValAla: 7.032 ± 0.093
0.704ValCys: 0.704 ± 0.03
3.713ValAsp: 3.713 ± 0.073
4.291ValGlu: 4.291 ± 0.075
2.695ValPhe: 2.695 ± 0.055
4.609ValGly: 4.609 ± 0.073
1.438ValHis: 1.438 ± 0.043
4.396ValIle: 4.396 ± 0.067
3.068ValLys: 3.068 ± 0.067
7.027ValLeu: 7.027 ± 0.116
1.935ValMet: 1.935 ± 0.048
2.448ValAsn: 2.448 ± 0.052
3.254ValPro: 3.254 ± 0.065
2.178ValGln: 2.178 ± 0.049
4.276ValArg: 4.276 ± 0.063
4.279ValSer: 4.279 ± 0.081
3.945ValThr: 3.945 ± 0.08
5.193ValVal: 5.193 ± 0.089
0.769ValTrp: 0.769 ± 0.03
1.662ValTyr: 1.662 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
1.02TrpAla: 1.02 ± 0.037
0.128TrpCys: 0.128 ± 0.012
0.618TrpAsp: 0.618 ± 0.029
0.62TrpGlu: 0.62 ± 0.03
0.529TrpPhe: 0.529 ± 0.026
0.847TrpGly: 0.847 ± 0.033
0.359TrpHis: 0.359 ± 0.019
0.767TrpIle: 0.767 ± 0.026
0.586TrpLys: 0.586 ± 0.024
1.704TrpLeu: 1.704 ± 0.047
0.35TrpMet: 0.35 ± 0.019
0.527TrpAsn: 0.527 ± 0.027
0.541TrpPro: 0.541 ± 0.024
0.656TrpGln: 0.656 ± 0.028
0.943TrpArg: 0.943 ± 0.029
0.736TrpSer: 0.736 ± 0.03
0.606TrpThr: 0.606 ± 0.024
0.865TrpVal: 0.865 ± 0.034
0.224TrpTrp: 0.224 ± 0.017
0.365TrpTyr: 0.365 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.572TyrAla: 2.572 ± 0.058
0.319TyrCys: 0.319 ± 0.021
1.479TyrAsp: 1.479 ± 0.056
1.45TyrGlu: 1.45 ± 0.041
1.278TyrPhe: 1.278 ± 0.042
2.262TyrGly: 2.262 ± 0.06
0.715TyrHis: 0.715 ± 0.03
1.34TyrIle: 1.34 ± 0.039
0.973TyrLys: 0.973 ± 0.031
3.069TyrLeu: 3.069 ± 0.067
0.469TyrMet: 0.469 ± 0.024
0.823TyrAsn: 0.823 ± 0.031
1.397TyrPro: 1.397 ± 0.037
1.152TyrGln: 1.152 ± 0.033
2.034TyrArg: 2.034 ± 0.049
1.689TyrSer: 1.689 ± 0.046
1.405TyrThr: 1.405 ± 0.04
1.798TyrVal: 1.798 ± 0.05
0.436TyrTrp: 0.436 ± 0.023
0.87TyrTyr: 0.87 ± 0.036
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3073 proteins (908958 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski