Amino acid dipepetide frequency for Streptomyces sp. Act143

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.276AlaAla: 21.276 ± 0.128
1.07AlaCys: 1.07 ± 0.02
8.454AlaAsp: 8.454 ± 0.058
8.814AlaGlu: 8.814 ± 0.075
3.541AlaPhe: 3.541 ± 0.038
12.845AlaGly: 12.845 ± 0.081
2.944AlaHis: 2.944 ± 0.037
3.382AlaIle: 3.382 ± 0.041
2.916AlaLys: 2.916 ± 0.042
14.584AlaLeu: 14.584 ± 0.096
2.447AlaMet: 2.447 ± 0.027
1.965AlaAsn: 1.965 ± 0.031
6.945AlaPro: 6.945 ± 0.067
3.841AlaGln: 3.841 ± 0.045
10.162AlaArg: 10.162 ± 0.089
5.945AlaSer: 5.945 ± 0.052
7.196AlaThr: 7.196 ± 0.06
12.341AlaVal: 12.341 ± 0.081
1.935AlaTrp: 1.935 ± 0.027
2.877AlaTyr: 2.877 ± 0.032
0.0AlaXaa: 0.0 ± 0.0
Cys
1.084CysAla: 1.084 ± 0.02
0.093CysCys: 0.093 ± 0.006
0.465CysAsp: 0.465 ± 0.014
0.414CysGlu: 0.414 ± 0.013
0.215CysPhe: 0.215 ± 0.01
0.956CysGly: 0.956 ± 0.019
0.187CysHis: 0.187 ± 0.008
0.16CysIle: 0.16 ± 0.008
0.119CysLys: 0.119 ± 0.006
0.756CysLeu: 0.756 ± 0.017
0.123CysMet: 0.123 ± 0.006
0.151CysAsn: 0.151 ± 0.008
0.473CysPro: 0.473 ± 0.015
0.17CysGln: 0.17 ± 0.009
0.582CysArg: 0.582 ± 0.014
0.418CysSer: 0.418 ± 0.013
0.565CysThr: 0.565 ± 0.015
0.68CysVal: 0.68 ± 0.015
0.12CysTrp: 0.12 ± 0.006
0.149CysTyr: 0.149 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.7AspAla: 7.7 ± 0.06
0.441AspCys: 0.441 ± 0.015
3.677AspAsp: 3.677 ± 0.043
3.806AspGlu: 3.806 ± 0.038
1.684AspPhe: 1.684 ± 0.027
6.467AspGly: 6.467 ± 0.061
1.436AspHis: 1.436 ± 0.022
1.928AspIle: 1.928 ± 0.027
1.279AspLys: 1.279 ± 0.024
6.235AspLeu: 6.235 ± 0.057
0.817AspMet: 0.817 ± 0.021
1.026AspAsn: 1.026 ± 0.021
4.374AspPro: 4.374 ± 0.044
1.565AspGln: 1.565 ± 0.026
4.828AspArg: 4.828 ± 0.054
2.622AspSer: 2.622 ± 0.032
3.286AspThr: 3.286 ± 0.039
4.811AspVal: 4.811 ± 0.038
1.115AspTrp: 1.115 ± 0.021
1.167AspTyr: 1.167 ± 0.02
0.0AspXaa: 0.0 ± 0.0
Glu
7.386GluAla: 7.386 ± 0.072
0.366GluCys: 0.366 ± 0.012
2.791GluAsp: 2.791 ± 0.037
3.555GluGlu: 3.555 ± 0.041
1.485GluPhe: 1.485 ± 0.022
4.288GluGly: 4.288 ± 0.044
1.493GluHis: 1.493 ± 0.023
2.241GluIle: 2.241 ± 0.032
1.537GluLys: 1.537 ± 0.028
6.797GluLeu: 6.797 ± 0.057
0.872GluMet: 0.872 ± 0.018
1.044GluAsn: 1.044 ± 0.019
3.346GluPro: 3.346 ± 0.041
2.243GluGln: 2.243 ± 0.037
5.257GluArg: 5.257 ± 0.052
2.488GluSer: 2.488 ± 0.031
2.963GluThr: 2.963 ± 0.036
4.396GluVal: 4.396 ± 0.049
0.803GluTrp: 0.803 ± 0.018
1.137GluTyr: 1.137 ± 0.019
0.0GluXaa: 0.0 ± 0.0
Phe
3.673PheAla: 3.673 ± 0.042
0.257PheCys: 0.257 ± 0.01
1.923PheAsp: 1.923 ± 0.028
1.437PheGlu: 1.437 ± 0.025
0.876PhePhe: 0.876 ± 0.021
2.985PheGly: 2.985 ± 0.037
0.624PheHis: 0.624 ± 0.014
0.684PheIle: 0.684 ± 0.017
0.566PheLys: 0.566 ± 0.016
2.63PheLeu: 2.63 ± 0.04
0.423PheMet: 0.423 ± 0.014
0.569PheAsn: 0.569 ± 0.016
1.383PhePro: 1.383 ± 0.024
0.7PheGln: 0.7 ± 0.015
1.855PheArg: 1.855 ± 0.027
1.451PheSer: 1.451 ± 0.027
2.04PheThr: 2.04 ± 0.029
2.289PheVal: 2.289 ± 0.029
0.438PheTrp: 0.438 ± 0.013
0.614PheTyr: 0.614 ± 0.014
0.0PheXaa: 0.0 ± 0.0
Gly
11.089GlyAla: 11.089 ± 0.075
0.83GlyCys: 0.83 ± 0.019
5.207GlyAsp: 5.207 ± 0.047
4.985GlyGlu: 4.985 ± 0.044
2.925GlyPhe: 2.925 ± 0.037
8.728GlyGly: 8.728 ± 0.082
2.309GlyHis: 2.309 ± 0.03
3.452GlyIle: 3.452 ± 0.039
2.486GlyLys: 2.486 ± 0.041
9.402GlyLeu: 9.402 ± 0.062
1.926GlyMet: 1.926 ± 0.025
1.808GlyAsn: 1.808 ± 0.034
5.014GlyPro: 5.014 ± 0.049
2.646GlyGln: 2.646 ± 0.038
7.493GlyArg: 7.493 ± 0.062
5.33GlySer: 5.33 ± 0.056
6.682GlyThr: 6.682 ± 0.061
7.602GlyVal: 7.602 ± 0.059
1.764GlyTrp: 1.764 ± 0.03
2.296GlyTyr: 2.296 ± 0.035
0.001GlyXaa: 0.001 ± 0.001
His
2.709HisAla: 2.709 ± 0.037
0.218HisCys: 0.218 ± 0.01
1.362HisAsp: 1.362 ± 0.024
1.247HisGlu: 1.247 ± 0.024
0.663HisPhe: 0.663 ± 0.014
2.415HisGly: 2.415 ± 0.034
0.711HisHis: 0.711 ± 0.018
0.676HisIle: 0.676 ± 0.017
0.36HisLys: 0.36 ± 0.01
2.458HisLeu: 2.458 ± 0.033
0.341HisMet: 0.341 ± 0.011
0.376HisAsn: 0.376 ± 0.013
1.826HisPro: 1.826 ± 0.029
0.651HisGln: 0.651 ± 0.015
2.091HisArg: 2.091 ± 0.03
0.986HisSer: 0.986 ± 0.019
1.382HisThr: 1.382 ± 0.021
1.708HisVal: 1.708 ± 0.024
0.392HisTrp: 0.392 ± 0.013
0.513HisTyr: 0.513 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.714IleAla: 4.714 ± 0.049
0.276IleCys: 0.276 ± 0.01
2.206IleAsp: 2.206 ± 0.032
1.928IleGlu: 1.928 ± 0.031
0.671IlePhe: 0.671 ± 0.017
3.518IleGly: 3.518 ± 0.041
0.626IleHis: 0.626 ± 0.016
0.84IleIle: 0.84 ± 0.021
0.752IleLys: 0.752 ± 0.02
2.379IleLeu: 2.379 ± 0.035
0.445IleMet: 0.445 ± 0.015
0.696IleAsn: 0.696 ± 0.017
1.738IlePro: 1.738 ± 0.027
0.735IleGln: 0.735 ± 0.017
2.178IleArg: 2.178 ± 0.031
1.646IleSer: 1.646 ± 0.023
2.233IleThr: 2.233 ± 0.03
2.71IleVal: 2.71 ± 0.034
0.376IleTrp: 0.376 ± 0.012
0.541IleTyr: 0.541 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
3.128LysAla: 3.128 ± 0.043
0.135LysCys: 0.135 ± 0.008
1.405LysAsp: 1.405 ± 0.027
1.233LysGlu: 1.233 ± 0.028
0.495LysPhe: 0.495 ± 0.013
1.915LysGly: 1.915 ± 0.03
0.436LysHis: 0.436 ± 0.013
0.883LysIle: 0.883 ± 0.022
0.951LysLys: 0.951 ± 0.025
2.085LysLeu: 2.085 ± 0.031
0.4LysMet: 0.4 ± 0.014
0.551LysAsn: 0.551 ± 0.016
1.36LysPro: 1.36 ± 0.027
0.731LysGln: 0.731 ± 0.021
1.41LysArg: 1.41 ± 0.022
1.236LysSer: 1.236 ± 0.026
1.307LysThr: 1.307 ± 0.027
2.029LysVal: 2.029 ± 0.032
0.297LysTrp: 0.297 ± 0.011
0.5LysTyr: 0.5 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
15.068LeuAla: 15.068 ± 0.097
0.856LeuCys: 0.856 ± 0.02
6.675LeuAsp: 6.675 ± 0.054
4.615LeuGlu: 4.615 ± 0.051
2.648LeuPhe: 2.648 ± 0.036
9.304LeuGly: 9.304 ± 0.067
2.347LeuHis: 2.347 ± 0.032
3.227LeuIle: 3.227 ± 0.041
2.232LeuLys: 2.232 ± 0.032
11.527LeuLeu: 11.527 ± 0.099
1.652LeuMet: 1.652 ± 0.029
1.72LeuAsn: 1.72 ± 0.028
6.436LeuPro: 6.436 ± 0.054
2.2LeuGln: 2.2 ± 0.032
8.492LeuArg: 8.492 ± 0.072
5.26LeuSer: 5.26 ± 0.044
7.143LeuThr: 7.143 ± 0.052
8.838LeuVal: 8.838 ± 0.065
1.359LeuTrp: 1.359 ± 0.024
1.932LeuTyr: 1.932 ± 0.029
0.0LeuXaa: 0.0 ± 0.0
Met
2.216MetAla: 2.216 ± 0.025
0.132MetCys: 0.132 ± 0.007
0.862MetAsp: 0.862 ± 0.018
0.743MetGlu: 0.743 ± 0.016
0.451MetPhe: 0.451 ± 0.016
1.291MetGly: 1.291 ± 0.026
0.344MetHis: 0.344 ± 0.011
0.647MetIle: 0.647 ± 0.015
0.42MetLys: 0.42 ± 0.013
1.664MetLeu: 1.664 ± 0.021
0.287MetMet: 0.287 ± 0.011
0.454MetAsn: 0.454 ± 0.013
1.108MetPro: 1.108 ± 0.02
0.45MetGln: 0.45 ± 0.012
1.38MetArg: 1.38 ± 0.025
1.294MetSer: 1.294 ± 0.021
1.57MetThr: 1.57 ± 0.026
1.254MetVal: 1.254 ± 0.023
0.218MetTrp: 0.218 ± 0.009
0.32MetTyr: 0.32 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
2.262AsnAla: 2.262 ± 0.033
0.174AsnCys: 0.174 ± 0.008
0.98AsnAsp: 0.98 ± 0.02
0.807AsnGlu: 0.807 ± 0.014
0.495AsnPhe: 0.495 ± 0.014
2.022AsnGly: 2.022 ± 0.038
0.401AsnHis: 0.401 ± 0.012
0.668AsnIle: 0.668 ± 0.018
0.435AsnLys: 0.435 ± 0.015
1.671AsnLeu: 1.671 ± 0.029
0.293AsnMet: 0.293 ± 0.011
0.482AsnAsn: 0.482 ± 0.017
1.366AsnPro: 1.366 ± 0.024
0.555AsnGln: 0.555 ± 0.016
1.247AsnArg: 1.247 ± 0.02
1.022AsnSer: 1.022 ± 0.022
1.185AsnThr: 1.185 ± 0.024
1.387AsnVal: 1.387 ± 0.022
0.318AsnTrp: 0.318 ± 0.011
0.45AsnTyr: 0.45 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.412ProAla: 8.412 ± 0.074
0.334ProCys: 0.334 ± 0.012
4.458ProAsp: 4.458 ± 0.041
4.297ProGlu: 4.297 ± 0.047
1.503ProPhe: 1.503 ± 0.024
6.399ProGly: 6.399 ± 0.057
1.434ProHis: 1.434 ± 0.024
1.213ProIle: 1.213 ± 0.024
1.181ProLys: 1.181 ± 0.021
5.351ProLeu: 5.351 ± 0.048
0.978ProMet: 0.978 ± 0.019
0.91ProAsn: 0.91 ± 0.02
3.454ProPro: 3.454 ± 0.058
1.765ProGln: 1.765 ± 0.031
3.851ProArg: 3.851 ± 0.042
3.152ProSer: 3.152 ± 0.038
3.299ProThr: 3.299 ± 0.042
5.547ProVal: 5.547 ± 0.047
0.893ProTrp: 0.893 ± 0.019
1.466ProTyr: 1.466 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.732GlnAla: 3.732 ± 0.044
0.167GlnCys: 0.167 ± 0.007
1.446GlnAsp: 1.446 ± 0.025
1.483GlnGlu: 1.483 ± 0.027
0.692GlnPhe: 0.692 ± 0.016
2.38GlnGly: 2.38 ± 0.03
0.662GlnHis: 0.662 ± 0.016
1.099GlnIle: 1.099 ± 0.022
0.64GlnLys: 0.64 ± 0.017
3.058GlnLeu: 3.058 ± 0.034
0.495GlnMet: 0.495 ± 0.013
0.538GlnAsn: 0.538 ± 0.017
1.71GlnPro: 1.71 ± 0.033
1.308GlnGln: 1.308 ± 0.033
2.28GlnArg: 2.28 ± 0.034
1.269GlnSer: 1.269 ± 0.022
1.398GlnThr: 1.398 ± 0.023
2.414GlnVal: 2.414 ± 0.031
0.512GlnTrp: 0.512 ± 0.014
0.654GlnTyr: 0.654 ± 0.015
0.0GlnXaa: 0.0 ± 0.0
Arg
9.729ArgAla: 9.729 ± 0.08
0.566ArgCys: 0.566 ± 0.014
4.236ArgAsp: 4.236 ± 0.046
4.619ArgGlu: 4.619 ± 0.051
2.253ArgPhe: 2.253 ± 0.03
5.655ArgGly: 5.655 ± 0.055
2.117ArgHis: 2.117 ± 0.032
3.107ArgIle: 3.107 ± 0.034
1.621ArgLys: 1.621 ± 0.025
8.77ArgLeu: 8.77 ± 0.068
1.665ArgMet: 1.665 ± 0.025
1.318ArgAsn: 1.318 ± 0.021
4.799ArgPro: 4.799 ± 0.056
2.26ArgGln: 2.26 ± 0.034
7.564ArgArg: 7.564 ± 0.078
3.848ArgSer: 3.848 ± 0.041
5.442ArgThr: 5.442 ± 0.056
5.84ArgVal: 5.84 ± 0.049
1.353ArgTrp: 1.353 ± 0.026
1.792ArgTyr: 1.792 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
6.773SerAla: 6.773 ± 0.058
0.426SerCys: 0.426 ± 0.013
2.726SerAsp: 2.726 ± 0.037
2.432SerGlu: 2.432 ± 0.034
1.514SerPhe: 1.514 ± 0.025
5.932SerGly: 5.932 ± 0.065
0.971SerHis: 0.971 ± 0.02
1.369SerIle: 1.369 ± 0.027
1.079SerLys: 1.079 ± 0.02
4.813SerLeu: 4.813 ± 0.041
1.077SerMet: 1.077 ± 0.018
0.906SerAsn: 0.906 ± 0.017
3.096SerPro: 3.096 ± 0.036
1.257SerGln: 1.257 ± 0.023
3.572SerArg: 3.572 ± 0.038
2.909SerSer: 2.909 ± 0.044
3.083SerThr: 3.083 ± 0.039
4.32SerVal: 4.32 ± 0.038
0.906SerTrp: 0.906 ± 0.018
1.334SerTyr: 1.334 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
9.128ThrAla: 9.128 ± 0.069
0.443ThrCys: 0.443 ± 0.012
3.905ThrAsp: 3.905 ± 0.046
3.288ThrGlu: 3.288 ± 0.034
1.641ThrPhe: 1.641 ± 0.027
6.803ThrGly: 6.803 ± 0.057
1.261ThrHis: 1.261 ± 0.025
1.712ThrIle: 1.712 ± 0.029
1.251ThrLys: 1.251 ± 0.026
5.893ThrLeu: 5.893 ± 0.05
0.936ThrMet: 0.936 ± 0.018
1.068ThrAsn: 1.068 ± 0.023
4.33ThrPro: 4.33 ± 0.048
1.42ThrGln: 1.42 ± 0.026
3.937ThrArg: 3.937 ± 0.036
3.307ThrSer: 3.307 ± 0.042
4.013ThrThr: 4.013 ± 0.056
6.276ThrVal: 6.276 ± 0.059
0.992ThrTrp: 0.992 ± 0.024
1.494ThrTyr: 1.494 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
10.833ValAla: 10.833 ± 0.064
0.766ValCys: 0.766 ± 0.018
4.909ValAsp: 4.909 ± 0.043
4.676ValGlu: 4.676 ± 0.049
2.478ValPhe: 2.478 ± 0.031
6.562ValGly: 6.562 ± 0.052
2.007ValHis: 2.007 ± 0.027
2.817ValIle: 2.817 ± 0.034
1.818ValLys: 1.818 ± 0.026
9.545ValLeu: 9.545 ± 0.067
1.382ValMet: 1.382 ± 0.022
1.73ValAsn: 1.73 ± 0.03
5.282ValPro: 5.282 ± 0.051
2.124ValGln: 2.124 ± 0.028
7.228ValArg: 7.228 ± 0.061
4.333ValSer: 4.333 ± 0.037
5.898ValThr: 5.898 ± 0.055
8.116ValVal: 8.116 ± 0.056
1.202ValTrp: 1.202 ± 0.024
1.644ValTyr: 1.644 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.72TrpAla: 1.72 ± 0.03
0.159TrpCys: 0.159 ± 0.007
0.869TrpAsp: 0.869 ± 0.019
0.737TrpGlu: 0.737 ± 0.014
0.521TrpPhe: 0.521 ± 0.014
1.101TrpGly: 1.101 ± 0.021
0.392TrpHis: 0.392 ± 0.012
0.579TrpIle: 0.579 ± 0.015
0.377TrpLys: 0.377 ± 0.012
1.822TrpLeu: 1.822 ± 0.027
0.295TrpMet: 0.295 ± 0.009
0.461TrpAsn: 0.461 ± 0.012
0.792TrpPro: 0.792 ± 0.018
0.669TrpGln: 0.669 ± 0.017
1.333TrpArg: 1.333 ± 0.024
0.974TrpSer: 0.974 ± 0.021
1.148TrpThr: 1.148 ± 0.024
1.026TrpVal: 1.026 ± 0.022
0.374TrpTrp: 0.374 ± 0.012
0.406TrpTyr: 0.406 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.849TyrAla: 2.849 ± 0.031
0.173TyrCys: 0.173 ± 0.008
1.71TyrAsp: 1.71 ± 0.03
1.37TyrGlu: 1.37 ± 0.025
0.665TyrPhe: 0.665 ± 0.015
2.406TyrGly: 2.406 ± 0.035
0.386TyrHis: 0.386 ± 0.013
0.524TyrIle: 0.524 ± 0.013
0.463TyrLys: 0.463 ± 0.015
2.094TyrLeu: 2.094 ± 0.029
0.248TyrMet: 0.248 ± 0.009
0.448TyrAsn: 0.448 ± 0.015
1.069TyrPro: 1.069 ± 0.021
0.644TyrGln: 0.644 ± 0.014
1.812TyrArg: 1.812 ± 0.027
1.004TyrSer: 1.004 ± 0.022
1.266TyrThr: 1.266 ± 0.027
1.764TyrVal: 1.764 ± 0.025
0.389TyrTrp: 0.389 ± 0.013
0.488TyrTyr: 0.488 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.002XaaXaa: 0.002 ± 0.001
Statistics based on 8401 proteins (2773813 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski