Thermotalea metallivorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Thermotalea

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3013 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140LAK4|A0A140LAK4_9CLOT GTP 3' 8-cyclase OS=Thermotalea metallivorans OX=520762 GN=moaA PE=3 SV=1
MM1 pKa = 6.96NRR3 pKa = 11.84KK4 pKa = 9.83DD5 pKa = 3.27IFKK8 pKa = 10.72LLEE11 pKa = 3.67QEE13 pKa = 5.19LYY15 pKa = 11.33GDD17 pKa = 4.16TNCPDD22 pKa = 3.67AEE24 pKa = 4.68LGCEE28 pKa = 3.57NCKK31 pKa = 10.82YY32 pKa = 10.46IEE34 pKa = 4.27ACIKK38 pKa = 10.64LDD40 pKa = 5.07DD41 pKa = 4.4LSEE44 pKa = 5.31DD45 pKa = 3.0IGIIDD50 pKa = 3.56VAA52 pKa = 4.23

Molecular weight: 5.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140KZG4|A0A140KZG4_9CLOT Quinolinate phosphoribosyltransferase [decarboxylating] OS=Thermotalea metallivorans OX=520762 GN=nadC PE=3 SV=1
MM1 pKa = 7.43LPSLALGLIFGLLIFFAGILIMSKK25 pKa = 10.71AVTNTANQQFRR36 pKa = 11.84KK37 pKa = 8.67WVSFFTGNPISGILVGTIITGITQSSSGTTVMVVSLVNSGVMNLYY82 pKa = 9.91QATAIIMGANIGTTFTAQLISFDD105 pKa = 3.77FFSIIPHH112 pKa = 6.21LMFIGVLLYY121 pKa = 10.28HH122 pKa = 6.41LHH124 pKa = 5.94FHH126 pKa = 5.53PTIRR130 pKa = 11.84QLGRR134 pKa = 11.84FTFGFSLLFLGIQLMSRR151 pKa = 11.84SLEE154 pKa = 4.05PLKK157 pKa = 11.32DD158 pKa = 3.33MADD161 pKa = 3.97FQQLMLSIEE170 pKa = 3.94NRR172 pKa = 11.84PFRR175 pKa = 11.84GVLIGLFTTAIIQSSSTGVAILQSLSLQGLIRR207 pKa = 11.84ITQAMPIILGQNIGTCFTTILSALAVDD234 pKa = 4.35KK235 pKa = 10.96NGKK238 pKa = 8.53RR239 pKa = 11.84AAIIHH244 pKa = 6.2LLFNIVGVILLYY256 pKa = 10.43PFMEE260 pKa = 4.05QFSYY264 pKa = 11.07CIARR268 pKa = 11.84LTPLNPVRR276 pKa = 11.84QIANAHH282 pKa = 5.87TIFNIINTIVLIPFIRR298 pKa = 11.84QMVWISQKK306 pKa = 10.31IIKK309 pKa = 9.67

Molecular weight: 34.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3013

0

3013

875152

29

2486

290.5

32.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.902 ± 0.046

1.073 ± 0.02

5.207 ± 0.031

7.687 ± 0.048

4.123 ± 0.035

7.096 ± 0.039

2.046 ± 0.021

8.936 ± 0.047

7.9 ± 0.036

9.172 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.055 ± 0.024

4.398 ± 0.028

3.409 ± 0.026

3.27 ± 0.023

4.49 ± 0.036

5.064 ± 0.032

4.779 ± 0.03

6.723 ± 0.042

0.834 ± 0.016

3.835 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski