Amino acid dipepetide frequency for CrAssphage sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.806AlaAla: 3.806 ± 0.571
0.435AlaCys: 0.435 ± 0.131
3.226AlaAsp: 3.226 ± 0.424
3.045AlaGlu: 3.045 ± 0.331
2.247AlaPhe: 2.247 ± 0.295
3.226AlaGly: 3.226 ± 0.398
0.652AlaHis: 0.652 ± 0.123
5.111AlaIle: 5.111 ± 0.509
5.546AlaLys: 5.546 ± 0.777
3.951AlaLeu: 3.951 ± 0.442
1.124AlaMet: 1.124 ± 0.192
4.386AlaAsn: 4.386 ± 0.596
1.522AlaPro: 1.522 ± 0.228
2.356AlaGln: 2.356 ± 0.352
2.61AlaArg: 2.61 ± 0.407
3.733AlaSer: 3.733 ± 0.482
3.371AlaThr: 3.371 ± 0.361
3.298AlaVal: 3.298 ± 0.43
0.58AlaTrp: 0.58 ± 0.126
2.03AlaTyr: 2.03 ± 0.226
0.0AlaXaa: 0.0 ± 0.0
Cys
0.616CysAla: 0.616 ± 0.152
0.362CysCys: 0.362 ± 0.152
0.761CysAsp: 0.761 ± 0.187
0.689CysGlu: 0.689 ± 0.211
0.507CysPhe: 0.507 ± 0.19
0.58CysGly: 0.58 ± 0.179
0.399CysHis: 0.399 ± 0.155
1.559CysIle: 1.559 ± 0.364
1.087CysLys: 1.087 ± 0.326
0.652CysLeu: 0.652 ± 0.187
0.109CysMet: 0.109 ± 0.067
1.015CysAsn: 1.015 ± 0.215
0.181CysPro: 0.181 ± 0.1
0.435CysGln: 0.435 ± 0.151
0.58CysArg: 0.58 ± 0.132
0.507CysSer: 0.507 ± 0.164
0.544CysThr: 0.544 ± 0.152
0.797CysVal: 0.797 ± 0.16
0.145CysTrp: 0.145 ± 0.064
0.725CysTyr: 0.725 ± 0.18
0.0CysXaa: 0.0 ± 0.0
Asp
4.458AspAla: 4.458 ± 0.41
1.015AspCys: 1.015 ± 0.238
3.987AspAsp: 3.987 ± 0.371
3.806AspGlu: 3.806 ± 0.333
3.298AspPhe: 3.298 ± 0.331
3.733AspGly: 3.733 ± 0.369
0.616AspHis: 0.616 ± 0.126
5.799AspIle: 5.799 ± 0.364
6.271AspLys: 6.271 ± 0.488
3.951AspLeu: 3.951 ± 0.363
1.704AspMet: 1.704 ± 0.314
4.132AspAsn: 4.132 ± 0.375
1.377AspPro: 1.377 ± 0.19
0.834AspGln: 0.834 ± 0.249
2.32AspArg: 2.32 ± 0.209
3.262AspSer: 3.262 ± 0.389
3.443AspThr: 3.443 ± 0.355
3.951AspVal: 3.951 ± 0.366
0.797AspTrp: 0.797 ± 0.199
4.168AspTyr: 4.168 ± 0.342
0.0AspXaa: 0.0 ± 0.0
Glu
4.096GluAla: 4.096 ± 0.508
0.652GluCys: 0.652 ± 0.17
3.878GluAsp: 3.878 ± 0.425
5.364GluGlu: 5.364 ± 0.538
2.972GluPhe: 2.972 ± 0.33
2.791GluGly: 2.791 ± 0.269
1.269GluHis: 1.269 ± 0.178
5.437GluIle: 5.437 ± 0.332
4.096GluLys: 4.096 ± 0.385
6.633GluLeu: 6.633 ± 0.557
1.45GluMet: 1.45 ± 0.21
5.546GluAsn: 5.546 ± 0.519
0.906GluPro: 0.906 ± 0.166
2.791GluGln: 2.791 ± 0.55
3.081GluArg: 3.081 ± 0.331
3.842GluSer: 3.842 ± 0.606
3.335GluThr: 3.335 ± 0.381
3.516GluVal: 3.516 ± 0.363
0.507GluTrp: 0.507 ± 0.148
3.081GluTyr: 3.081 ± 0.379
0.0GluXaa: 0.0 ± 0.0
Phe
2.102PheAla: 2.102 ± 0.372
0.435PheCys: 0.435 ± 0.16
2.755PheAsp: 2.755 ± 0.311
2.284PheGlu: 2.284 ± 0.256
1.522PhePhe: 1.522 ± 0.214
2.61PheGly: 2.61 ± 0.314
0.616PheHis: 0.616 ± 0.211
3.697PheIle: 3.697 ± 0.375
3.371PheLys: 3.371 ± 0.321
2.465PheLeu: 2.465 ± 0.309
1.124PheMet: 1.124 ± 0.179
4.096PheAsn: 4.096 ± 0.419
0.87PhePro: 0.87 ± 0.177
0.87PheGln: 0.87 ± 0.162
1.74PheArg: 1.74 ± 0.312
2.827PheSer: 2.827 ± 0.367
3.48PheThr: 3.48 ± 0.483
2.9PheVal: 2.9 ± 0.205
0.326PheTrp: 0.326 ± 0.105
2.066PheTyr: 2.066 ± 0.328
0.0PheXaa: 0.0 ± 0.0
Gly
2.972GlyAla: 2.972 ± 0.465
0.652GlyCys: 0.652 ± 0.158
2.718GlyAsp: 2.718 ± 0.378
3.552GlyGlu: 3.552 ± 0.466
2.284GlyPhe: 2.284 ± 0.326
3.443GlyGly: 3.443 ± 0.48
0.797GlyHis: 0.797 ± 0.199
4.277GlyIle: 4.277 ± 0.355
3.915GlyLys: 3.915 ± 0.427
3.987GlyLeu: 3.987 ± 0.369
1.124GlyMet: 1.124 ± 0.219
4.277GlyAsn: 4.277 ± 0.384
0.362GlyPro: 0.362 ± 0.13
1.414GlyGln: 1.414 ± 0.228
2.211GlyArg: 2.211 ± 0.259
3.226GlySer: 3.226 ± 0.315
3.987GlyThr: 3.987 ± 0.359
4.06GlyVal: 4.06 ± 0.429
0.544GlyTrp: 0.544 ± 0.107
2.791GlyTyr: 2.791 ± 0.446
0.0GlyXaa: 0.0 ± 0.0
His
0.834HisAla: 0.834 ± 0.156
0.29HisCys: 0.29 ± 0.127
0.834HisAsp: 0.834 ± 0.151
0.652HisGlu: 0.652 ± 0.139
0.725HisPhe: 0.725 ± 0.124
0.797HisGly: 0.797 ± 0.17
0.217HisHis: 0.217 ± 0.099
1.341HisIle: 1.341 ± 0.206
1.45HisLys: 1.45 ± 0.243
1.595HisLeu: 1.595 ± 0.24
0.181HisMet: 0.181 ± 0.082
1.051HisAsn: 1.051 ± 0.239
0.616HisPro: 0.616 ± 0.142
0.399HisGln: 0.399 ± 0.095
0.652HisArg: 0.652 ± 0.152
1.124HisSer: 1.124 ± 0.246
0.979HisThr: 0.979 ± 0.255
0.942HisVal: 0.942 ± 0.158
0.109HisTrp: 0.109 ± 0.065
1.124HisTyr: 1.124 ± 0.219
0.0HisXaa: 0.0 ± 0.0
Ile
4.893IleAla: 4.893 ± 0.378
0.797IleCys: 0.797 ± 0.199
6.524IleAsp: 6.524 ± 0.614
4.893IleGlu: 4.893 ± 0.431
2.972IlePhe: 2.972 ± 0.527
3.878IleGly: 3.878 ± 0.346
1.704IleHis: 1.704 ± 0.237
7.358IleIle: 7.358 ± 0.957
6.706IleLys: 6.706 ± 0.552
5.219IleLeu: 5.219 ± 0.371
1.486IleMet: 1.486 ± 0.249
8.663IleAsn: 8.663 ± 0.679
3.19IlePro: 3.19 ± 0.341
2.501IleGln: 2.501 ± 0.335
3.697IleArg: 3.697 ± 0.358
5.147IleSer: 5.147 ± 0.439
6.633IleThr: 6.633 ± 0.653
4.313IleVal: 4.313 ± 0.527
0.326IleTrp: 0.326 ± 0.114
4.06IleTyr: 4.06 ± 0.486
0.0IleXaa: 0.0 ± 0.0
Lys
4.857LysAla: 4.857 ± 0.587
1.196LysCys: 1.196 ± 0.28
6.089LysAsp: 6.089 ± 0.563
7.865LysGlu: 7.865 ± 0.718
3.951LysPhe: 3.951 ± 0.384
3.987LysGly: 3.987 ± 0.422
1.595LysHis: 1.595 ± 0.22
5.654LysIle: 5.654 ± 0.46
5.727LysLys: 5.727 ± 0.475
7.467LysLeu: 7.467 ± 0.46
1.631LysMet: 1.631 ± 0.34
5.401LysAsn: 5.401 ± 0.412
1.595LysPro: 1.595 ± 0.256
3.588LysGln: 3.588 ± 0.324
3.407LysArg: 3.407 ± 0.386
5.401LysSer: 5.401 ± 0.5
4.93LysThr: 4.93 ± 0.465
3.987LysVal: 3.987 ± 0.489
0.544LysTrp: 0.544 ± 0.142
4.205LysTyr: 4.205 ± 0.43
0.0LysXaa: 0.0 ± 0.0
Leu
4.023LeuAla: 4.023 ± 0.425
0.87LeuCys: 0.87 ± 0.233
5.328LeuAsp: 5.328 ± 0.513
4.132LeuGlu: 4.132 ± 0.347
3.588LeuPhe: 3.588 ± 0.442
4.023LeuGly: 4.023 ± 0.284
1.45LeuHis: 1.45 ± 0.247
5.654LeuIle: 5.654 ± 0.574
6.524LeuLys: 6.524 ± 0.517
5.256LeuLeu: 5.256 ± 0.545
1.704LeuMet: 1.704 ± 0.271
6.379LeuAsn: 6.379 ± 0.457
2.573LeuPro: 2.573 ± 0.409
2.646LeuGln: 2.646 ± 0.355
2.646LeuArg: 2.646 ± 0.394
5.328LeuSer: 5.328 ± 0.435
4.893LeuThr: 4.893 ± 0.435
3.625LeuVal: 3.625 ± 0.508
0.544LeuTrp: 0.544 ± 0.15
3.878LeuTyr: 3.878 ± 0.328
0.0LeuXaa: 0.0 ± 0.0
Met
1.486MetAla: 1.486 ± 0.26
0.254MetCys: 0.254 ± 0.105
0.87MetAsp: 0.87 ± 0.177
1.341MetGlu: 1.341 ± 0.239
1.269MetPhe: 1.269 ± 0.281
0.979MetGly: 0.979 ± 0.174
0.254MetHis: 0.254 ± 0.119
1.522MetIle: 1.522 ± 0.247
1.812MetLys: 1.812 ± 0.246
2.102MetLeu: 2.102 ± 0.214
0.725MetMet: 0.725 ± 0.258
1.305MetAsn: 1.305 ± 0.196
0.834MetPro: 0.834 ± 0.189
0.979MetGln: 0.979 ± 0.166
1.124MetArg: 1.124 ± 0.227
1.704MetSer: 1.704 ± 0.28
1.414MetThr: 1.414 ± 0.273
1.087MetVal: 1.087 ± 0.189
0.145MetTrp: 0.145 ± 0.087
1.015MetTyr: 1.015 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
3.915AsnAla: 3.915 ± 0.44
0.979AsnCys: 0.979 ± 0.303
4.35AsnAsp: 4.35 ± 0.431
4.857AsnGlu: 4.857 ± 0.541
2.32AsnPhe: 2.32 ± 0.38
5.546AsnGly: 5.546 ± 0.569
0.906AsnHis: 0.906 ± 0.178
7.793AsnIle: 7.793 ± 0.638
7.213AsnLys: 7.213 ± 0.465
5.292AsnLeu: 5.292 ± 0.338
1.885AsnMet: 1.885 ± 0.288
8.228AsnAsn: 8.228 ± 0.545
2.863AsnPro: 2.863 ± 0.391
2.175AsnGln: 2.175 ± 0.307
2.863AsnArg: 2.863 ± 0.292
6.053AsnSer: 6.053 ± 0.562
5.763AsnThr: 5.763 ± 0.561
5.074AsnVal: 5.074 ± 0.513
0.362AsnTrp: 0.362 ± 0.103
4.676AsnTyr: 4.676 ± 0.463
0.0AsnXaa: 0.0 ± 0.0
Pro
1.087ProAla: 1.087 ± 0.218
0.471ProCys: 0.471 ± 0.155
1.776ProAsp: 1.776 ± 0.287
1.849ProGlu: 1.849 ± 0.362
1.196ProPhe: 1.196 ± 0.192
1.051ProGly: 1.051 ± 0.195
0.435ProHis: 0.435 ± 0.126
2.211ProIle: 2.211 ± 0.338
1.812ProLys: 1.812 ± 0.206
1.885ProLeu: 1.885 ± 0.212
0.725ProMet: 0.725 ± 0.152
2.501ProAsn: 2.501 ± 0.462
0.29ProPro: 0.29 ± 0.094
0.689ProGln: 0.689 ± 0.192
1.015ProArg: 1.015 ± 0.177
1.885ProSer: 1.885 ± 0.27
1.74ProThr: 1.74 ± 0.278
1.812ProVal: 1.812 ± 0.209
0.217ProTrp: 0.217 ± 0.093
1.232ProTyr: 1.232 ± 0.22
0.0ProXaa: 0.0 ± 0.0
Gln
2.247GlnAla: 2.247 ± 0.419
0.145GlnCys: 0.145 ± 0.064
1.812GlnAsp: 1.812 ± 0.323
2.537GlnGlu: 2.537 ± 0.388
1.16GlnPhe: 1.16 ± 0.172
1.232GlnGly: 1.232 ± 0.266
0.544GlnHis: 0.544 ± 0.107
3.008GlnIle: 3.008 ± 0.309
1.812GlnLys: 1.812 ± 0.335
3.262GlnLeu: 3.262 ± 0.318
0.652GlnMet: 0.652 ± 0.182
2.066GlnAsn: 2.066 ± 0.262
0.58GlnPro: 0.58 ± 0.121
1.812GlnGln: 1.812 ± 0.475
1.45GlnArg: 1.45 ± 0.263
1.885GlnSer: 1.885 ± 0.265
1.921GlnThr: 1.921 ± 0.249
1.631GlnVal: 1.631 ± 0.177
0.544GlnTrp: 0.544 ± 0.109
1.957GlnTyr: 1.957 ± 0.243
0.0GlnXaa: 0.0 ± 0.0
Arg
2.066ArgAla: 2.066 ± 0.356
0.58ArgCys: 0.58 ± 0.191
2.429ArgAsp: 2.429 ± 0.339
2.32ArgGlu: 2.32 ± 0.252
1.631ArgPhe: 1.631 ± 0.219
2.32ArgGly: 2.32 ± 0.314
0.652ArgHis: 0.652 ± 0.162
3.045ArgIle: 3.045 ± 0.387
4.168ArgLys: 4.168 ± 0.443
3.661ArgLeu: 3.661 ± 0.414
1.051ArgMet: 1.051 ± 0.187
3.153ArgAsn: 3.153 ± 0.267
0.87ArgPro: 0.87 ± 0.166
1.704ArgGln: 1.704 ± 0.238
1.849ArgArg: 1.849 ± 0.3
2.284ArgSer: 2.284 ± 0.276
1.812ArgThr: 1.812 ± 0.239
1.885ArgVal: 1.885 ± 0.256
0.29ArgTrp: 0.29 ± 0.088
2.102ArgTyr: 2.102 ± 0.332
0.0ArgXaa: 0.0 ± 0.0
Ser
3.552SerAla: 3.552 ± 0.34
0.906SerCys: 0.906 ± 0.194
4.132SerAsp: 4.132 ± 0.387
3.77SerGlu: 3.77 ± 0.387
2.646SerPhe: 2.646 ± 0.313
3.19SerGly: 3.19 ± 0.366
0.979SerHis: 0.979 ± 0.193
5.908SerIle: 5.908 ± 0.531
5.727SerLys: 5.727 ± 0.468
4.785SerLeu: 4.785 ± 0.523
1.522SerMet: 1.522 ± 0.262
5.654SerAsn: 5.654 ± 0.467
1.921SerPro: 1.921 ± 0.278
1.776SerGln: 1.776 ± 0.22
2.32SerArg: 2.32 ± 0.284
4.531SerSer: 4.531 ± 0.409
4.531SerThr: 4.531 ± 0.386
3.697SerVal: 3.697 ± 0.342
0.507SerTrp: 0.507 ± 0.166
3.516SerTyr: 3.516 ± 0.382
0.0SerXaa: 0.0 ± 0.0
Thr
3.625ThrAla: 3.625 ± 0.407
0.471ThrCys: 0.471 ± 0.181
4.023ThrAsp: 4.023 ± 0.416
4.313ThrGlu: 4.313 ± 0.538
2.863ThrPhe: 2.863 ± 0.357
2.863ThrGly: 2.863 ± 0.342
0.906ThrHis: 0.906 ± 0.207
6.162ThrIle: 6.162 ± 0.432
5.292ThrLys: 5.292 ± 0.366
5.002ThrLeu: 5.002 ± 0.432
1.377ThrMet: 1.377 ± 0.25
4.93ThrAsn: 4.93 ± 0.477
2.139ThrPro: 2.139 ± 0.218
2.102ThrGln: 2.102 ± 0.297
1.849ThrArg: 1.849 ± 0.274
4.785ThrSer: 4.785 ± 0.396
4.386ThrThr: 4.386 ± 0.447
3.552ThrVal: 3.552 ± 0.368
0.544ThrTrp: 0.544 ± 0.133
3.262ThrTyr: 3.262 ± 0.354
0.0ThrXaa: 0.0 ± 0.0
Val
2.791ValAla: 2.791 ± 0.399
0.906ValCys: 0.906 ± 0.159
3.951ValAsp: 3.951 ± 0.458
4.531ValGlu: 4.531 ± 0.445
2.284ValPhe: 2.284 ± 0.272
3.045ValGly: 3.045 ± 0.293
0.761ValHis: 0.761 ± 0.117
4.35ValIle: 4.35 ± 0.453
4.893ValLys: 4.893 ± 0.403
3.625ValLeu: 3.625 ± 0.359
1.486ValMet: 1.486 ± 0.215
4.857ValAsn: 4.857 ± 0.405
1.74ValPro: 1.74 ± 0.266
1.486ValGln: 1.486 ± 0.214
2.139ValArg: 2.139 ± 0.311
3.733ValSer: 3.733 ± 0.426
3.806ValThr: 3.806 ± 0.356
3.552ValVal: 3.552 ± 0.361
0.471ValTrp: 0.471 ± 0.146
2.356ValTyr: 2.356 ± 0.286
0.0ValXaa: 0.0 ± 0.0
Trp
0.29TrpAla: 0.29 ± 0.1
0.109TrpCys: 0.109 ± 0.059
0.362TrpAsp: 0.362 ± 0.128
0.399TrpGlu: 0.399 ± 0.116
0.58TrpPhe: 0.58 ± 0.146
0.362TrpGly: 0.362 ± 0.117
0.145TrpHis: 0.145 ± 0.067
0.652TrpIle: 0.652 ± 0.139
0.725TrpLys: 0.725 ± 0.122
0.834TrpLeu: 0.834 ± 0.203
0.217TrpMet: 0.217 ± 0.087
0.797TrpAsn: 0.797 ± 0.227
0.0TrpPro: 0.0 ± 0.0
0.181TrpGln: 0.181 ± 0.088
0.362TrpArg: 0.362 ± 0.099
0.761TrpSer: 0.761 ± 0.148
0.399TrpThr: 0.399 ± 0.098
0.29TrpVal: 0.29 ± 0.115
0.145TrpTrp: 0.145 ± 0.068
0.58TrpTyr: 0.58 ± 0.132
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.537TyrAla: 2.537 ± 0.221
0.725TyrCys: 0.725 ± 0.169
3.117TyrAsp: 3.117 ± 0.294
3.045TyrGlu: 3.045 ± 0.303
2.139TyrPhe: 2.139 ± 0.355
2.936TyrGly: 2.936 ± 0.357
1.015TyrHis: 1.015 ± 0.201
4.458TyrIle: 4.458 ± 0.478
4.93TyrLys: 4.93 ± 0.509
3.443TyrLeu: 3.443 ± 0.389
0.906TyrMet: 0.906 ± 0.237
4.676TyrAsn: 4.676 ± 0.434
1.45TyrPro: 1.45 ± 0.246
1.522TyrGln: 1.522 ± 0.209
1.957TyrArg: 1.957 ± 0.332
3.552TyrSer: 3.552 ± 0.305
3.081TyrThr: 3.081 ± 0.394
2.791TyrVal: 2.791 ± 0.305
0.544TyrTrp: 0.544 ± 0.166
2.429TyrTyr: 2.429 ± 0.282
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (27590 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski