Amino acid dipepetide frequency for Rothia phage Spartoi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.843AlaAla: 14.843 ± 1.369
0.54AlaCys: 0.54 ± 0.275
4.768AlaAsp: 4.768 ± 0.746
7.647AlaGlu: 7.647 ± 1.201
4.498AlaPhe: 4.498 ± 1.303
8.546AlaGly: 8.546 ± 1.259
1.979AlaHis: 1.979 ± 0.434
7.017AlaIle: 7.017 ± 1.211
6.117AlaLys: 6.117 ± 0.997
8.726AlaLeu: 8.726 ± 0.81
2.519AlaMet: 2.519 ± 0.507
5.218AlaAsn: 5.218 ± 0.642
5.128AlaPro: 5.128 ± 0.979
3.688AlaGln: 3.688 ± 0.545
5.937AlaArg: 5.937 ± 0.799
6.207AlaSer: 6.207 ± 1.195
5.398AlaThr: 5.398 ± 0.688
8.276AlaVal: 8.276 ± 1.019
2.159AlaTrp: 2.159 ± 0.414
2.069AlaTyr: 2.069 ± 0.424
0.0AlaXaa: 0.0 ± 0.0
Cys
0.63CysAla: 0.63 ± 0.247
0.09CysCys: 0.09 ± 0.1
0.36CysAsp: 0.36 ± 0.168
0.27CysGlu: 0.27 ± 0.159
0.0CysPhe: 0.0 ± 0.0
1.08CysGly: 1.08 ± 0.354
0.18CysHis: 0.18 ± 0.114
0.54CysIle: 0.54 ± 0.234
0.54CysLys: 0.54 ± 0.194
0.54CysLeu: 0.54 ± 0.282
0.27CysMet: 0.27 ± 0.196
0.36CysAsn: 0.36 ± 0.174
0.54CysPro: 0.54 ± 0.226
0.45CysGln: 0.45 ± 0.287
0.63CysArg: 0.63 ± 0.244
0.45CysSer: 0.45 ± 0.204
0.27CysThr: 0.27 ± 0.132
0.27CysVal: 0.27 ± 0.174
0.27CysTrp: 0.27 ± 0.147
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
5.398AspAla: 5.398 ± 0.756
0.54AspCys: 0.54 ± 0.196
1.979AspAsp: 1.979 ± 0.414
3.508AspGlu: 3.508 ± 0.646
1.889AspPhe: 1.889 ± 0.429
4.858AspGly: 4.858 ± 0.778
0.81AspHis: 0.81 ± 0.264
2.699AspIle: 2.699 ± 0.529
2.429AspLys: 2.429 ± 0.502
3.868AspLeu: 3.868 ± 0.688
1.439AspMet: 1.439 ± 0.309
1.889AspAsn: 1.889 ± 0.485
1.439AspPro: 1.439 ± 0.373
1.979AspGln: 1.979 ± 0.471
2.159AspArg: 2.159 ± 0.337
3.418AspSer: 3.418 ± 0.621
2.519AspThr: 2.519 ± 0.466
3.059AspVal: 3.059 ± 0.346
1.169AspTrp: 1.169 ± 0.304
1.169AspTyr: 1.169 ± 0.303
0.0AspXaa: 0.0 ± 0.0
Glu
9.266GluAla: 9.266 ± 0.832
0.45GluCys: 0.45 ± 0.213
2.699GluAsp: 2.699 ± 0.427
5.038GluGlu: 5.038 ± 0.713
2.069GluPhe: 2.069 ± 0.44
5.937GluGly: 5.937 ± 0.967
1.619GluHis: 1.619 ± 0.45
4.138GluIle: 4.138 ± 0.583
4.498GluLys: 4.498 ± 0.865
6.747GluLeu: 6.747 ± 0.863
1.529GluMet: 1.529 ± 0.309
3.329GluAsn: 3.329 ± 0.512
2.609GluPro: 2.609 ± 0.493
3.149GluGln: 3.149 ± 0.405
3.418GluArg: 3.418 ± 0.5
3.059GluSer: 3.059 ± 0.642
4.588GluThr: 4.588 ± 0.706
3.868GluVal: 3.868 ± 0.489
1.259GluTrp: 1.259 ± 0.351
1.709GluTyr: 1.709 ± 0.343
0.0GluXaa: 0.0 ± 0.0
Phe
3.239PheAla: 3.239 ± 0.534
0.63PheCys: 0.63 ± 0.206
1.799PheAsp: 1.799 ± 0.468
2.969PheGlu: 2.969 ± 0.396
1.619PhePhe: 1.619 ± 0.347
2.789PheGly: 2.789 ± 0.62
0.54PheHis: 0.54 ± 0.174
1.799PheIle: 1.799 ± 0.353
1.439PheLys: 1.439 ± 0.371
2.339PheLeu: 2.339 ± 0.548
0.45PheMet: 0.45 ± 0.199
1.169PheAsn: 1.169 ± 0.251
1.709PhePro: 1.709 ± 0.499
1.169PheGln: 1.169 ± 0.323
1.349PheArg: 1.349 ± 0.324
2.249PheSer: 2.249 ± 0.341
2.339PheThr: 2.339 ± 0.37
1.979PheVal: 1.979 ± 0.316
0.45PheTrp: 0.45 ± 0.182
0.9PheTyr: 0.9 ± 0.29
0.0PheXaa: 0.0 ± 0.0
Gly
7.377GlyAla: 7.377 ± 1.243
0.81GlyCys: 0.81 ± 0.263
3.958GlyAsp: 3.958 ± 0.517
4.948GlyGlu: 4.948 ± 0.568
3.059GlyPhe: 3.059 ± 0.464
7.467GlyGly: 7.467 ± 0.899
0.99GlyHis: 0.99 ± 0.317
7.377GlyIle: 7.377 ± 1.405
5.578GlyLys: 5.578 ± 0.798
5.668GlyLeu: 5.668 ± 0.683
1.889GlyMet: 1.889 ± 0.44
3.778GlyAsn: 3.778 ± 0.567
2.159GlyPro: 2.159 ± 0.583
2.429GlyGln: 2.429 ± 0.432
3.239GlyArg: 3.239 ± 0.514
4.228GlySer: 4.228 ± 0.602
5.668GlyThr: 5.668 ± 0.911
5.847GlyVal: 5.847 ± 0.738
2.429GlyTrp: 2.429 ± 0.477
2.609GlyTyr: 2.609 ± 0.422
0.0GlyXaa: 0.0 ± 0.0
His
1.709HisAla: 1.709 ± 0.38
0.36HisCys: 0.36 ± 0.189
0.72HisAsp: 0.72 ± 0.253
1.349HisGlu: 1.349 ± 0.38
0.45HisPhe: 0.45 ± 0.244
1.979HisGly: 1.979 ± 0.321
0.27HisHis: 0.27 ± 0.157
1.259HisIle: 1.259 ± 0.375
0.63HisLys: 0.63 ± 0.197
1.529HisLeu: 1.529 ± 0.364
0.27HisMet: 0.27 ± 0.129
0.27HisAsn: 0.27 ± 0.153
1.08HisPro: 1.08 ± 0.369
0.36HisGln: 0.36 ± 0.162
1.439HisArg: 1.439 ± 0.368
0.99HisSer: 0.99 ± 0.247
1.169HisThr: 1.169 ± 0.402
0.63HisVal: 0.63 ± 0.235
0.72HisTrp: 0.72 ± 0.207
0.63HisTyr: 0.63 ± 0.256
0.0HisXaa: 0.0 ± 0.0
Ile
5.668IleAla: 5.668 ± 0.732
0.45IleCys: 0.45 ± 0.197
2.609IleAsp: 2.609 ± 0.435
4.408IleGlu: 4.408 ± 0.644
0.99IlePhe: 0.99 ± 0.242
5.128IleGly: 5.128 ± 0.809
1.259IleHis: 1.259 ± 0.337
3.598IleIle: 3.598 ± 0.562
4.498IleLys: 4.498 ± 0.904
3.688IleLeu: 3.688 ± 0.678
1.259IleMet: 1.259 ± 0.323
2.249IleAsn: 2.249 ± 0.449
3.868IlePro: 3.868 ± 0.515
2.699IleGln: 2.699 ± 0.51
3.508IleArg: 3.508 ± 0.54
3.418IleSer: 3.418 ± 0.589
4.048IleThr: 4.048 ± 0.591
4.408IleVal: 4.408 ± 0.772
0.99IleTrp: 0.99 ± 0.263
2.249IleTyr: 2.249 ± 0.503
0.0IleXaa: 0.0 ± 0.0
Lys
7.377LysAla: 7.377 ± 0.824
0.27LysCys: 0.27 ± 0.139
2.879LysAsp: 2.879 ± 0.788
4.228LysGlu: 4.228 ± 0.707
1.619LysPhe: 1.619 ± 0.383
4.678LysGly: 4.678 ± 0.682
1.529LysHis: 1.529 ± 0.37
3.598LysIle: 3.598 ± 0.675
4.048LysLys: 4.048 ± 0.781
4.138LysLeu: 4.138 ± 0.734
1.529LysMet: 1.529 ± 0.362
2.159LysAsn: 2.159 ± 0.438
4.228LysPro: 4.228 ± 0.713
1.529LysGln: 1.529 ± 0.441
3.598LysArg: 3.598 ± 0.662
2.879LysSer: 2.879 ± 0.661
4.588LysThr: 4.588 ± 0.599
3.239LysVal: 3.239 ± 0.647
0.81LysTrp: 0.81 ± 0.284
0.99LysTyr: 0.99 ± 0.309
0.0LysXaa: 0.0 ± 0.0
Leu
8.816LeuAla: 8.816 ± 1.032
0.54LeuCys: 0.54 ± 0.226
4.588LeuAsp: 4.588 ± 0.63
5.488LeuGlu: 5.488 ± 0.797
1.799LeuPhe: 1.799 ± 0.397
5.668LeuGly: 5.668 ± 0.8
1.169LeuHis: 1.169 ± 0.281
3.598LeuIle: 3.598 ± 0.702
5.668LeuLys: 5.668 ± 0.751
5.668LeuLeu: 5.668 ± 0.767
1.529LeuMet: 1.529 ± 0.42
3.329LeuAsn: 3.329 ± 0.513
4.138LeuPro: 4.138 ± 0.736
2.879LeuGln: 2.879 ± 0.51
5.398LeuArg: 5.398 ± 0.83
5.308LeuSer: 5.308 ± 0.707
5.757LeuThr: 5.757 ± 0.624
4.588LeuVal: 4.588 ± 0.753
0.63LeuTrp: 0.63 ± 0.224
1.259LeuTyr: 1.259 ± 0.353
0.0LeuXaa: 0.0 ± 0.0
Met
2.429MetAla: 2.429 ± 0.51
0.09MetCys: 0.09 ± 0.1
0.9MetAsp: 0.9 ± 0.246
1.799MetGlu: 1.799 ± 0.392
0.63MetPhe: 0.63 ± 0.22
1.799MetGly: 1.799 ± 0.361
0.27MetHis: 0.27 ± 0.156
1.169MetIle: 1.169 ± 0.257
1.169MetLys: 1.169 ± 0.36
1.709MetLeu: 1.709 ± 0.549
0.63MetMet: 0.63 ± 0.214
1.08MetAsn: 1.08 ± 0.315
1.349MetPro: 1.349 ± 0.306
0.81MetGln: 0.81 ± 0.279
1.889MetArg: 1.889 ± 0.383
1.979MetSer: 1.979 ± 0.334
1.799MetThr: 1.799 ± 0.374
0.99MetVal: 0.99 ± 0.252
0.0MetTrp: 0.0 ± 0.0
0.09MetTyr: 0.09 ± 0.078
0.0MetXaa: 0.0 ± 0.0
Asn
3.149AsnAla: 3.149 ± 0.588
0.18AsnCys: 0.18 ± 0.113
1.889AsnAsp: 1.889 ± 0.439
2.969AsnGlu: 2.969 ± 0.624
1.259AsnPhe: 1.259 ± 0.351
4.678AsnGly: 4.678 ± 0.823
0.36AsnHis: 0.36 ± 0.157
2.159AsnIle: 2.159 ± 0.515
2.069AsnLys: 2.069 ± 0.46
3.868AsnLeu: 3.868 ± 0.675
1.349AsnMet: 1.349 ± 0.336
1.259AsnAsn: 1.259 ± 0.364
2.789AsnPro: 2.789 ± 0.375
1.799AsnGln: 1.799 ± 0.493
2.159AsnArg: 2.159 ± 0.479
2.699AsnSer: 2.699 ± 0.35
1.799AsnThr: 1.799 ± 0.322
1.439AsnVal: 1.439 ± 0.303
0.45AsnTrp: 0.45 ± 0.184
0.36AsnTyr: 0.36 ± 0.199
0.0AsnXaa: 0.0 ± 0.0
Pro
6.837ProAla: 6.837 ± 0.934
0.45ProCys: 0.45 ± 0.193
2.879ProAsp: 2.879 ± 0.534
3.868ProGlu: 3.868 ± 0.765
1.799ProPhe: 1.799 ± 0.373
4.768ProGly: 4.768 ± 0.924
0.99ProHis: 0.99 ± 0.311
2.429ProIle: 2.429 ± 0.45
2.249ProLys: 2.249 ± 0.54
4.048ProLeu: 4.048 ± 0.511
0.99ProMet: 0.99 ± 0.331
2.069ProAsn: 2.069 ± 0.418
2.789ProPro: 2.789 ± 0.766
2.069ProGln: 2.069 ± 0.329
2.969ProArg: 2.969 ± 0.647
3.329ProSer: 3.329 ± 0.489
1.349ProThr: 1.349 ± 0.372
3.778ProVal: 3.778 ± 0.638
0.63ProTrp: 0.63 ± 0.211
0.9ProTyr: 0.9 ± 0.259
0.0ProXaa: 0.0 ± 0.0
Gln
4.588GlnAla: 4.588 ± 0.556
0.09GlnCys: 0.09 ± 0.096
2.249GlnAsp: 2.249 ± 0.491
2.159GlnGlu: 2.159 ± 0.425
1.08GlnPhe: 1.08 ± 0.361
2.429GlnGly: 2.429 ± 0.503
0.63GlnHis: 0.63 ± 0.217
1.799GlnIle: 1.799 ± 0.431
2.339GlnLys: 2.339 ± 0.48
4.048GlnLeu: 4.048 ± 0.667
0.45GlnMet: 0.45 ± 0.199
1.259GlnAsn: 1.259 ± 0.287
2.249GlnPro: 2.249 ± 0.536
2.429GlnGln: 2.429 ± 0.48
2.069GlnArg: 2.069 ± 0.45
1.529GlnSer: 1.529 ± 0.371
2.249GlnThr: 2.249 ± 0.353
3.418GlnVal: 3.418 ± 0.521
0.72GlnTrp: 0.72 ± 0.222
0.45GlnTyr: 0.45 ± 0.199
0.0GlnXaa: 0.0 ± 0.0
Arg
5.398ArgAla: 5.398 ± 0.612
0.36ArgCys: 0.36 ± 0.21
2.969ArgAsp: 2.969 ± 0.484
4.138ArgGlu: 4.138 ± 0.782
1.709ArgPhe: 1.709 ± 0.331
4.048ArgGly: 4.048 ± 0.745
0.99ArgHis: 0.99 ± 0.347
3.418ArgIle: 3.418 ± 0.511
3.329ArgLys: 3.329 ± 0.676
4.318ArgLeu: 4.318 ± 0.522
1.619ArgMet: 1.619 ± 0.478
2.249ArgAsn: 2.249 ± 0.373
3.508ArgPro: 3.508 ± 0.653
1.529ArgGln: 1.529 ± 0.336
4.048ArgArg: 4.048 ± 0.892
2.879ArgSer: 2.879 ± 0.649
2.969ArgThr: 2.969 ± 0.539
4.048ArgVal: 4.048 ± 0.538
0.9ArgTrp: 0.9 ± 0.306
1.529ArgTyr: 1.529 ± 0.429
0.0ArgXaa: 0.0 ± 0.0
Ser
7.737SerAla: 7.737 ± 0.906
0.54SerCys: 0.54 ± 0.234
2.879SerAsp: 2.879 ± 0.491
5.398SerGlu: 5.398 ± 1.479
2.699SerPhe: 2.699 ± 0.649
4.948SerGly: 4.948 ± 0.509
1.259SerHis: 1.259 ± 0.372
3.598SerIle: 3.598 ± 0.564
3.059SerLys: 3.059 ± 0.437
4.228SerLeu: 4.228 ± 0.456
0.81SerMet: 0.81 ± 0.202
1.799SerAsn: 1.799 ± 0.348
2.159SerPro: 2.159 ± 0.368
1.799SerGln: 1.799 ± 0.412
3.418SerArg: 3.418 ± 0.6
2.699SerSer: 2.699 ± 0.479
3.329SerThr: 3.329 ± 0.405
3.598SerVal: 3.598 ± 0.638
0.36SerTrp: 0.36 ± 0.175
1.529SerTyr: 1.529 ± 0.466
0.0SerXaa: 0.0 ± 0.0
Thr
8.006ThrAla: 8.006 ± 0.824
0.18ThrCys: 0.18 ± 0.143
2.159ThrAsp: 2.159 ± 0.382
3.059ThrGlu: 3.059 ± 0.58
1.529ThrPhe: 1.529 ± 0.36
3.868ThrGly: 3.868 ± 0.639
1.259ThrHis: 1.259 ± 0.32
5.038ThrIle: 5.038 ± 0.562
3.598ThrLys: 3.598 ± 0.567
5.668ThrLeu: 5.668 ± 0.651
0.99ThrMet: 0.99 ± 0.297
1.709ThrAsn: 1.709 ± 0.48
3.329ThrPro: 3.329 ± 0.6
2.609ThrGln: 2.609 ± 0.434
2.609ThrArg: 2.609 ± 0.511
3.778ThrSer: 3.778 ± 0.544
3.239ThrThr: 3.239 ± 0.604
5.218ThrVal: 5.218 ± 0.644
0.9ThrTrp: 0.9 ± 0.23
1.349ThrTyr: 1.349 ± 0.321
0.0ThrXaa: 0.0 ± 0.0
Val
5.488ValAla: 5.488 ± 0.659
0.81ValCys: 0.81 ± 0.451
3.508ValAsp: 3.508 ± 0.644
4.498ValGlu: 4.498 ± 0.554
2.609ValPhe: 2.609 ± 0.477
3.329ValGly: 3.329 ± 0.506
0.72ValHis: 0.72 ± 0.21
4.138ValIle: 4.138 ± 0.604
4.408ValLys: 4.408 ± 0.632
4.138ValLeu: 4.138 ± 0.792
1.619ValMet: 1.619 ± 0.432
2.429ValAsn: 2.429 ± 0.454
4.228ValPro: 4.228 ± 0.841
2.789ValGln: 2.789 ± 0.534
3.868ValArg: 3.868 ± 0.896
4.318ValSer: 4.318 ± 0.629
5.128ValThr: 5.128 ± 0.904
4.498ValVal: 4.498 ± 0.671
1.979ValTrp: 1.979 ± 0.397
1.799ValTyr: 1.799 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
1.259TrpAla: 1.259 ± 0.315
0.18TrpCys: 0.18 ± 0.122
0.9TrpAsp: 0.9 ± 0.203
1.979TrpGlu: 1.979 ± 0.4
0.72TrpPhe: 0.72 ± 0.226
1.169TrpGly: 1.169 ± 0.306
0.54TrpHis: 0.54 ± 0.242
0.45TrpIle: 0.45 ± 0.165
0.9TrpLys: 0.9 ± 0.228
1.349TrpLeu: 1.349 ± 0.35
0.81TrpMet: 0.81 ± 0.26
0.72TrpAsn: 0.72 ± 0.212
0.36TrpPro: 0.36 ± 0.194
1.169TrpGln: 1.169 ± 0.296
1.08TrpArg: 1.08 ± 0.407
0.9TrpSer: 0.9 ± 0.242
0.63TrpThr: 0.63 ± 0.259
1.709TrpVal: 1.709 ± 0.46
0.09TrpTrp: 0.09 ± 0.086
0.36TrpTyr: 0.36 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.069TyrAla: 2.069 ± 0.447
0.18TyrCys: 0.18 ± 0.138
1.529TyrAsp: 1.529 ± 0.368
1.259TyrGlu: 1.259 ± 0.448
0.9TyrPhe: 0.9 ± 0.284
2.339TyrGly: 2.339 ± 0.486
0.45TyrHis: 0.45 ± 0.206
1.169TyrIle: 1.169 ± 0.32
1.349TyrLys: 1.349 ± 0.443
1.529TyrLeu: 1.529 ± 0.417
0.54TyrMet: 0.54 ± 0.236
0.36TyrAsn: 0.36 ± 0.17
1.529TyrPro: 1.529 ± 0.373
0.9TyrGln: 0.9 ± 0.29
1.259TyrArg: 1.259 ± 0.363
1.529TyrSer: 1.529 ± 0.422
1.259TyrThr: 1.259 ± 0.386
1.439TyrVal: 1.439 ± 0.304
0.36TyrTrp: 0.36 ± 0.141
0.72TyrTyr: 0.72 ± 0.248
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (11117 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski