CRESS virus sp. ctfsu28

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; unclassified Cressdnaviricota; CRESS viruses

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A5Q2W9N6|A0A5Q2W9N6_9VIRU ATP-dependent helicase Rep OS=CRESS virus sp. ctfsu28 OX=2656685 PE=3 SV=1
MM1 pKa = 7.0AHH3 pKa = 7.3KK4 pKa = 10.23RR5 pKa = 11.84KK6 pKa = 9.16QWFYY10 pKa = 10.19GSNSVRR16 pKa = 11.84RR17 pKa = 11.84SVRR20 pKa = 11.84QQEE23 pKa = 4.33DD24 pKa = 4.24GNTPEE29 pKa = 5.17LKK31 pKa = 10.08MYY33 pKa = 9.7DD34 pKa = 3.59QSVLDD39 pKa = 3.95TLIGTGTSWSGADD52 pKa = 4.01MDD54 pKa = 4.97PSSNTLFSPNLGVDD68 pKa = 3.41MGQRR72 pKa = 11.84IGRR75 pKa = 11.84RR76 pKa = 11.84CAVVGLDD83 pKa = 3.16MNGFIDD89 pKa = 5.79CNDD92 pKa = 2.98QVLAVGADD100 pKa = 3.47PAAICRR106 pKa = 11.84IIVFQDD112 pKa = 3.26TQTKK116 pKa = 9.83GVQALGNLVMEE127 pKa = 5.12AQTADD132 pKa = 3.36AFHH135 pKa = 7.22VITAFPSRR143 pKa = 11.84DD144 pKa = 3.36AIGRR148 pKa = 11.84FIILKK153 pKa = 10.4DD154 pKa = 3.4MVYY157 pKa = 11.17VMQNPAFSSLGSSGVTYY174 pKa = 9.98DD175 pKa = 3.25QQGIAKK181 pKa = 8.49WFSFRR186 pKa = 11.84IEE188 pKa = 3.7FDD190 pKa = 3.27EE191 pKa = 4.08PVIVHH196 pKa = 6.47FDD198 pKa = 3.21ASDD201 pKa = 3.24AGTVADD207 pKa = 3.75VLDD210 pKa = 3.97NSFHH214 pKa = 7.34VMASNSSSGLSPRR227 pKa = 11.84LTYY230 pKa = 7.32TTRR233 pKa = 11.84VYY235 pKa = 11.05YY236 pKa = 10.81YY237 pKa = 10.8DD238 pKa = 3.51FF239 pKa = 5.41

Molecular weight:
26.33 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A5Q2WAC3|A0A5Q2WAC3_9VIRU Capsid protein OS=CRESS virus sp. ctfsu28 OX=2656685 PE=4 SV=1
MM1 pKa = 7.48AGKK4 pKa = 10.01RR5 pKa = 11.84KK6 pKa = 10.11YY7 pKa = 10.03KK8 pKa = 10.48ADD10 pKa = 3.72YY11 pKa = 9.73KK12 pKa = 11.09KK13 pKa = 10.91DD14 pKa = 3.2GAGVKK19 pKa = 9.62KK20 pKa = 10.73YY21 pKa = 10.16KK22 pKa = 10.76GSASQQNYY30 pKa = 8.3IKK32 pKa = 10.48RR33 pKa = 11.84EE34 pKa = 4.02YY35 pKa = 10.47VPRR38 pKa = 11.84TPGGAVVSEE47 pKa = 4.38LKK49 pKa = 11.03VIDD52 pKa = 3.97SFLANTVLSAPTSWANTEE70 pKa = 3.93VDD72 pKa = 4.09PATLNCLFAPGPGTGLNQRR91 pKa = 11.84IGRR94 pKa = 11.84KK95 pKa = 9.08VAVKK99 pKa = 9.65KK100 pKa = 10.78LKK102 pKa = 10.19VRR104 pKa = 11.84GLITCASQVDD114 pKa = 4.04QTTPDD119 pKa = 3.05NGCQVRR125 pKa = 11.84LIFYY129 pKa = 10.65QDD131 pKa = 3.16MQTNGAQAQGEE142 pKa = 4.54DD143 pKa = 4.87LMDD146 pKa = 3.73TPTASVQGVFNSFQNTASFGRR167 pKa = 11.84FRR169 pKa = 11.84VLKK172 pKa = 10.4DD173 pKa = 2.89MCIEE177 pKa = 3.94INNPNISWDD186 pKa = 3.69GTNLEE191 pKa = 3.87QQGIVKK197 pKa = 10.11RR198 pKa = 11.84FKK200 pKa = 9.6FSKK203 pKa = 9.76VFKK206 pKa = 9.63EE207 pKa = 4.04PVVVHH212 pKa = 5.83YY213 pKa = 10.25NATGGTTVADD223 pKa = 3.62VVDD226 pKa = 4.15NSFHH230 pKa = 7.11AIAVVNSAALVPNLSYY246 pKa = 10.94VCRR249 pKa = 11.84VTYY252 pKa = 10.55TDD254 pKa = 3.1VV255 pKa = 3.0

Molecular weight:
27.79 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

816

239

322

272.0

30.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.985 ± 0.962

2.328 ± 0.538

7.23 ± 0.742

4.412 ± 1.444

4.044 ± 0.816

8.088 ± 0.142

1.593 ± 0.336

5.025 ± 0.429

6.618 ± 1.585

5.882 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.492

5.392 ± 0.556

3.676 ± 0.247

3.676 ± 1.295

5.27 ± 0.387

6.373 ± 1.103

5.515 ± 0.912

8.701 ± 1.173

1.961 ± 0.693

4.657 ± 0.881

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski