Chrysochromulina tobinii

Taxonomy: cellular organisms; Eukaryota; Haptista; Haptophyta; Prymnesiophyceae; Prymnesiales; Chrysochromulinaceae; Chrysochromulina

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16679 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M0K1I6|A0A0M0K1I6_9EUKA Coiled coil flagellar protein OS=Chrysochromulina tobinii OX=1460289 GN=Ctob_009394 PE=4 SV=1
MM1 pKa = 7.47SFADD5 pKa = 3.78AQQCATTCIGAPGYY19 pKa = 11.08ASDD22 pKa = 4.75CDD24 pKa = 3.94CAAEE28 pKa = 4.01FASNKK33 pKa = 9.44FATEE37 pKa = 3.7YY38 pKa = 10.52GYY40 pKa = 11.19DD41 pKa = 4.29FITVNGVSSSLTNGPMNVQVATEE64 pKa = 4.15TAQLVDD70 pKa = 3.8TFSTTTFGAVQACTRR85 pKa = 11.84GSLEE89 pKa = 3.96TFKK92 pKa = 11.29ARR94 pKa = 11.84VAAKK98 pKa = 9.84SLSNMTSTGAKK109 pKa = 8.93MDD111 pKa = 3.53PTLNDD116 pKa = 3.05VQTTLSIISYY126 pKa = 10.31LGGLLEE132 pKa = 4.29TDD134 pKa = 3.05AFEE137 pKa = 4.81ANSTIDD143 pKa = 3.12IAEE146 pKa = 4.56AAHH149 pKa = 5.93EE150 pKa = 4.26TDD152 pKa = 4.48PDD154 pKa = 3.99SMPYY158 pKa = 10.36ARR160 pKa = 11.84SSSTGASLVAIAPALLPGMLAVLGLLCAVLYY191 pKa = 10.47GAVQAFAAHH200 pKa = 6.94LGASALASIMGPPPPKK216 pKa = 10.08GRR218 pKa = 11.84RR219 pKa = 11.84AVSAAPASCRR229 pKa = 11.84PSARR233 pKa = 11.84RR234 pKa = 11.84GSAGRR239 pKa = 11.84FGSAPRR245 pKa = 11.84LVGFLLLALFVSATDD260 pKa = 3.77AQCMEE265 pKa = 4.58EE266 pKa = 4.01WCNVCTGEE274 pKa = 4.4CTSATGGEE282 pKa = 4.18LLGSSEE288 pKa = 4.94AVLQFLSSVLPSSSSGLTSSTTFSIATADD317 pKa = 3.39VDD319 pKa = 4.08GDD321 pKa = 3.75GDD323 pKa = 4.97LDD325 pKa = 3.87VLLGGSPTSRR335 pKa = 11.84VLLNAGNGTFPTSIEE350 pKa = 4.1LPGVSAEE357 pKa = 4.19TRR359 pKa = 11.84SIAAADD365 pKa = 3.88LDD367 pKa = 4.21GDD369 pKa = 3.95GDD371 pKa = 4.83LDD373 pKa = 3.87VLLGSSPSSVLLNAGDD389 pKa = 4.03GTFPTSITLPGDD401 pKa = 3.58GALTRR406 pKa = 11.84SITAADD412 pKa = 4.01LDD414 pKa = 4.23GDD416 pKa = 3.94GDD418 pKa = 4.83LDD420 pKa = 3.93VLIGNSGSPSRR431 pKa = 11.84VLLNAGDD438 pKa = 4.08GTFPASIEE446 pKa = 4.12LPGGSPLTYY455 pKa = 10.15SIAAADD461 pKa = 3.63VDD463 pKa = 4.1GDD465 pKa = 3.74GDD467 pKa = 4.8LDD469 pKa = 3.92VLIGNSNSPNRR480 pKa = 11.84VLLNAGDD487 pKa = 4.09GTFPTSITLPGGSAEE502 pKa = 4.36TNSIAAADD510 pKa = 3.73VDD512 pKa = 4.1GDD514 pKa = 3.74GDD516 pKa = 4.8LDD518 pKa = 3.92VLIGNSNSPNRR529 pKa = 11.84VLLNTGNGTFPTSIEE544 pKa = 4.17LPGSIAATQSIAAADD559 pKa = 3.63VDD561 pKa = 4.1GDD563 pKa = 3.77GDD565 pKa = 5.12LDD567 pKa = 3.87VLLGNNGSPSRR578 pKa = 11.84VLLNAGNGTFPTSITLPGGSLFTQSIAAADD608 pKa = 3.63VDD610 pKa = 4.1GDD612 pKa = 3.74GDD614 pKa = 4.8LDD616 pKa = 3.87VLIGNSQYY624 pKa = 10.96RR625 pKa = 11.84NRR627 pKa = 11.84MLLNAGGTFQTIIQLPGPGIVSTHH651 pKa = 6.58SIAAADD657 pKa = 4.29LDD659 pKa = 4.12SDD661 pKa = 3.96GDD663 pKa = 4.15LDD665 pKa = 4.27VLIGNSGSPSQVLLNAGDD683 pKa = 3.99GTFPASITLPGGSPFTQSVAAADD706 pKa = 3.67VDD708 pKa = 3.96GDD710 pKa = 3.74GDD712 pKa = 4.8LDD714 pKa = 3.85VLIGNSYY721 pKa = 10.75SPSLVLLNAGDD732 pKa = 4.08GTFPTSIEE740 pKa = 4.19LPGGTGDD747 pKa = 3.5TRR749 pKa = 11.84SIAAADD755 pKa = 3.54VDD757 pKa = 4.1GDD759 pKa = 3.77GDD761 pKa = 4.83LDD763 pKa = 3.79VLLGISYY770 pKa = 10.53SPSLVLLNAGDD781 pKa = 4.08GTFPTSITLPGGSPEE796 pKa = 4.28TNSIAAADD804 pKa = 3.73VDD806 pKa = 4.1GDD808 pKa = 3.71GDD810 pKa = 4.7LDD812 pKa = 3.91VLVGYY817 pKa = 9.93SLVLNTGNGTFPTRR831 pKa = 11.84ITLPGGSPATQSIAAADD848 pKa = 3.63VDD850 pKa = 4.1GDD852 pKa = 3.77GDD854 pKa = 5.12LDD856 pKa = 3.75VLLGNFGSPSQVLLNAGDD874 pKa = 4.07GTFPTSMEE882 pKa = 4.12LPGGPRR888 pKa = 11.84EE889 pKa = 4.29ATFSIAAADD898 pKa = 3.94LDD900 pKa = 4.16GDD902 pKa = 4.11GDD904 pKa = 4.47SDD906 pKa = 4.8LLLGNSGNPCRR917 pKa = 11.84VLLNAGSRR925 pKa = 11.84SGGITFEE932 pKa = 4.4TIIEE936 pKa = 4.44INTGTRR942 pKa = 11.84IYY944 pKa = 10.51EE945 pKa = 4.07PSPLTNSIVAADD957 pKa = 3.64VDD959 pKa = 4.17GDD961 pKa = 3.94GDD963 pKa = 5.55LDD965 pKa = 4.5LLLANSNNYY974 pKa = 8.84MNLKK978 pKa = 10.22SSLVLLNAGDD988 pKa = 4.02GTFPTIIKK996 pKa = 10.25LIGGSADD1003 pKa = 3.6TNSITDD1009 pKa = 3.52TSSIAAADD1017 pKa = 3.67VDD1019 pKa = 4.26SDD1021 pKa = 3.78GDD1023 pKa = 4.14LDD1025 pKa = 3.96VLLGGSPGHH1034 pKa = 6.88PSLVLLNAGNGTFPTSIEE1052 pKa = 4.09LPGGSEE1058 pKa = 4.14GTNSIAAADD1067 pKa = 3.73VDD1069 pKa = 4.1GDD1071 pKa = 3.77GDD1073 pKa = 4.97LDD1075 pKa = 3.83VLLGGNPSRR1084 pKa = 11.84VLLNAGGGTFPTSIEE1099 pKa = 4.16LPGSEE1104 pKa = 4.17GTNSIAAADD1113 pKa = 3.73VDD1115 pKa = 4.1GDD1117 pKa = 3.77GDD1119 pKa = 5.36LDD1121 pKa = 3.72VLLAGWFNRR1130 pKa = 11.84VLLNAGDD1137 pKa = 4.08GTFPASITLPSHH1149 pKa = 7.06DD1150 pKa = 3.5GSDD1153 pKa = 3.69RR1154 pKa = 11.84IVAADD1159 pKa = 4.07LDD1161 pKa = 4.37GDD1163 pKa = 3.96GDD1165 pKa = 4.99LDD1167 pKa = 3.75VLLGNRR1173 pKa = 11.84NSPSLVVLNAGNGTFPTSISLPGSSKK1199 pKa = 10.86LGISLAVADD1208 pKa = 4.51MDD1210 pKa = 4.8GDD1212 pKa = 3.98GDD1214 pKa = 4.79LDD1216 pKa = 3.95VLIGKK1221 pKa = 9.76YY1222 pKa = 10.23GLPSLLLLNAGDD1234 pKa = 4.11GTFPTSIEE1242 pKa = 4.14LPGGSAEE1249 pKa = 4.22TSSIAAADD1257 pKa = 3.55VDD1259 pKa = 4.1GDD1261 pKa = 3.74GDD1263 pKa = 4.8LDD1265 pKa = 3.82VLIGNRR1271 pKa = 11.84NSPSLVLPFIRR1282 pKa = 11.84CSDD1285 pKa = 3.58PGTARR1290 pKa = 11.84SRR1292 pKa = 11.84YY1293 pKa = 8.97GNGCVRR1299 pKa = 11.84CPAPSSRR1306 pKa = 11.84RR1307 pKa = 11.84DD1308 pKa = 3.64DD1309 pKa = 3.96VSDD1312 pKa = 3.41VCYY1315 pKa = 10.57EE1316 pKa = 4.07CDD1318 pKa = 3.37EE1319 pKa = 4.93HH1320 pKa = 8.75SHH1322 pKa = 6.93LDD1324 pKa = 3.74PGSGEE1329 pKa = 4.19CVACTEE1335 pKa = 4.16GSEE1338 pKa = 4.2RR1339 pKa = 11.84LLGATRR1345 pKa = 11.84CSRR1348 pKa = 11.84CPVGKK1353 pKa = 9.94RR1354 pKa = 11.84HH1355 pKa = 6.19AATGTGCIPCSPGKK1369 pKa = 8.93FTSTSGSIACQPCTPGYY1386 pKa = 8.81MCIEE1390 pKa = 4.81GSSAPQPCPGGTHH1403 pKa = 7.48ANQTMLNLTGYY1414 pKa = 10.14LGSLSEE1420 pKa = 4.89CLICPVGTSCSVGSAVAVPCAPGTYY1445 pKa = 9.84NNRR1448 pKa = 11.84SGQEE1452 pKa = 3.89TCLKK1456 pKa = 9.59CAPGSFQEE1464 pKa = 4.37LAGEE1468 pKa = 4.87TSCSPCPAGYY1478 pKa = 10.42YY1479 pKa = 9.07CAEE1482 pKa = 4.32GAAAALPCPFGTHH1495 pKa = 5.86KK1496 pKa = 10.68NASLSVMTSVDD1507 pKa = 3.3QCVVCPVGTSCSVGSAVAVPCAPGTYY1533 pKa = 9.94NNQPRR1538 pKa = 11.84QEE1540 pKa = 4.19TCLKK1544 pKa = 9.76CAAGSFQEE1552 pKa = 4.16LAGRR1556 pKa = 11.84TSCNPCTPGYY1566 pKa = 10.16YY1567 pKa = 9.44CAEE1570 pKa = 4.42GAATPVPCPRR1580 pKa = 11.84GKK1582 pKa = 10.47YY1583 pKa = 9.16NNRR1586 pKa = 11.84TGLEE1590 pKa = 4.04FAVGCVPVGFGFWAPTGSDD1609 pKa = 3.78FPIPCPSGFSCPGYY1623 pKa = 10.88FNDD1626 pKa = 4.04NLTFGSQPLILPSGGLPPEE1645 pKa = 4.4VAVEE1649 pKa = 4.19TQTVEE1654 pKa = 4.0TATGSISLNTPLSSFLNDD1672 pKa = 3.01TALQLQYY1679 pKa = 10.25RR1680 pKa = 11.84QTIAAQLGIPWQSLQLDD1697 pKa = 4.8FSAAPPSVGRR1707 pKa = 11.84RR1708 pKa = 11.84LQTSSTIVTYY1718 pKa = 9.41TIVRR1722 pKa = 11.84TTTTVHH1728 pKa = 6.43EE1729 pKa = 4.81GADD1732 pKa = 3.61SVSQCVCKK1740 pKa = 10.72LGTFASLTRR1749 pKa = 11.84AEE1751 pKa = 4.67MMQATNDD1758 pKa = 3.66SSRR1761 pKa = 11.84VAADD1765 pKa = 3.09AFEE1768 pKa = 4.01

Molecular weight: 177.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M0J5Z7|A0A0M0J5Z7_9EUKA Membrane protein OS=Chrysochromulina tobinii OX=1460289 GN=Ctob_000966 PE=4 SV=1
MM1 pKa = 7.7PRR3 pKa = 11.84TSPEE7 pKa = 3.73PAQATAASRR16 pKa = 11.84SSSGGSPLPSMQLRR30 pKa = 11.84RR31 pKa = 11.84AALARR36 pKa = 11.84PPRR39 pKa = 11.84NSRR42 pKa = 11.84SRR44 pKa = 11.84LGVSRR49 pKa = 11.84STSRR53 pKa = 11.84TAATLTVKK61 pKa = 10.5PSQRR65 pKa = 11.84SRR67 pKa = 11.84ARR69 pKa = 11.84LGSILPAAAASHH81 pKa = 5.94GRR83 pKa = 11.84PASTRR88 pKa = 11.84TLMLRR93 pKa = 11.84SVISASSVGAGG104 pKa = 3.2

Molecular weight: 10.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16663

16

16679

7768616

10

10901

465.8

50.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.134 ± 0.042

1.573 ± 0.009

5.19 ± 0.013

6.665 ± 0.025

3.082 ± 0.013

7.575 ± 0.023

2.29 ± 0.009

3.438 ± 0.014

4.125 ± 0.025

10.169 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.009

2.423 ± 0.011

5.47 ± 0.018

3.529 ± 0.013

6.754 ± 0.023

6.892 ± 0.017

5.227 ± 0.014

6.706 ± 0.014

1.336 ± 0.008

2.066 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski