Proteus phage 2207-N35

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gorganvirus; unclassified Gorganvirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9SUV2|A0A6B9SUV2_9CAUD Putative DNA methylase OS=Proteus phage 2207-N35 OX=2692740 PE=3 SV=1
MM1 pKa = 7.75NYY3 pKa = 9.58TIVWNEE9 pKa = 3.58AKK11 pKa = 10.61NEE13 pKa = 3.87GVIFSGEE20 pKa = 3.98SAKK23 pKa = 10.47RR24 pKa = 11.84DD25 pKa = 2.86IKK27 pKa = 9.92MAKK30 pKa = 9.12TGDD33 pKa = 3.83SNGMYY38 pKa = 8.8STLAGEE44 pKa = 5.1FYY46 pKa = 9.94DD47 pKa = 3.91TYY49 pKa = 11.62GEE51 pKa = 4.37YY52 pKa = 10.47EE53 pKa = 4.08PCTSVSLSQPSEE65 pKa = 4.21DD66 pKa = 3.31MVKK69 pKa = 10.76AGVEE73 pKa = 4.07ALRR76 pKa = 11.84KK77 pKa = 9.47CDD79 pKa = 3.31PALIDD84 pKa = 3.62EE85 pKa = 4.83EE86 pKa = 4.7STIEE90 pKa = 4.27LIYY93 pKa = 10.86KK94 pKa = 10.63AMIEE98 pKa = 4.41KK99 pKa = 8.34LTCNPP104 pKa = 3.75

Molecular weight: 11.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9SRS6|A0A6B9SRS6_9CAUD Uncharacterized protein OS=Proteus phage 2207-N35 OX=2692740 PE=4 SV=1
MM1 pKa = 7.34YY2 pKa = 10.79GLTQEE7 pKa = 4.07QMKK10 pKa = 10.13IARR13 pKa = 11.84NDD15 pKa = 2.95IMRR18 pKa = 11.84NPCKK22 pKa = 10.01HH23 pKa = 6.49DD24 pKa = 3.52FAMKK28 pKa = 10.04CLKK31 pKa = 10.25DD32 pKa = 3.58AYY34 pKa = 11.09NMRR37 pKa = 11.84KK38 pKa = 9.54RR39 pKa = 11.84EE40 pKa = 3.88WNIINRR46 pKa = 11.84VLLNSKK52 pKa = 9.96FGKK55 pKa = 10.24

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

17020

31

1039

181.1

20.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.152 ± 0.457

1.533 ± 0.17

6.41 ± 0.189

6.845 ± 0.248

3.901 ± 0.187

6.522 ± 0.233

1.769 ± 0.187

7.133 ± 0.211

7.197 ± 0.249

8.061 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.908 ± 0.125

5.987 ± 0.231

3.467 ± 0.214

3.449 ± 0.384

4.747 ± 0.161

6.122 ± 0.175

5.852 ± 0.227

6.51 ± 0.243

1.481 ± 0.116

3.954 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski