Amino acid dipepetide frequency for Proteus phage 2207-N35

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.939AlaAla: 1.939 ± 0.609
0.529AlaCys: 0.529 ± 0.14
3.584AlaAsp: 3.584 ± 0.512
5.229AlaGlu: 5.229 ± 0.719
2.174AlaPhe: 2.174 ± 0.475
4.231AlaGly: 4.231 ± 0.725
1.234AlaHis: 1.234 ± 0.326
4.348AlaIle: 4.348 ± 0.51
4.701AlaLys: 4.701 ± 0.79
5.641AlaLeu: 5.641 ± 0.506
3.114AlaMet: 3.114 ± 0.458
3.761AlaAsn: 3.761 ± 0.907
1.998AlaPro: 1.998 ± 0.502
2.233AlaGln: 2.233 ± 0.482
2.527AlaArg: 2.527 ± 0.466
4.054AlaSer: 4.054 ± 0.587
3.408AlaThr: 3.408 ± 0.48
4.524AlaVal: 4.524 ± 0.466
0.705AlaTrp: 0.705 ± 0.199
1.586AlaTyr: 1.586 ± 0.306
0.0AlaXaa: 0.0 ± 0.0
Cys
0.529CysAla: 0.529 ± 0.208
0.529CysCys: 0.529 ± 0.196
1.41CysAsp: 1.41 ± 0.353
1.586CysGlu: 1.586 ± 0.278
0.646CysPhe: 0.646 ± 0.212
0.764CysGly: 0.764 ± 0.329
0.176CysHis: 0.176 ± 0.113
0.588CysIle: 0.588 ± 0.211
1.586CysLys: 1.586 ± 0.342
1.293CysLeu: 1.293 ± 0.268
0.705CysMet: 0.705 ± 0.172
0.94CysAsn: 0.94 ± 0.278
0.353CysPro: 0.353 ± 0.191
0.411CysGln: 0.411 ± 0.163
0.588CysArg: 0.588 ± 0.197
0.588CysSer: 0.588 ± 0.21
0.588CysThr: 0.588 ± 0.189
0.94CysVal: 0.94 ± 0.239
0.176CysTrp: 0.176 ± 0.095
0.94CysTyr: 0.94 ± 0.225
0.0CysXaa: 0.0 ± 0.0
Asp
4.113AspAla: 4.113 ± 0.447
0.999AspCys: 0.999 ± 0.257
4.524AspAsp: 4.524 ± 0.481
3.819AspGlu: 3.819 ± 0.355
2.057AspPhe: 2.057 ± 0.452
5.582AspGly: 5.582 ± 0.574
0.999AspHis: 0.999 ± 0.254
4.759AspIle: 4.759 ± 0.592
4.583AspLys: 4.583 ± 0.472
4.113AspLeu: 4.113 ± 0.689
1.763AspMet: 1.763 ± 0.306
4.172AspAsn: 4.172 ± 0.537
2.468AspPro: 2.468 ± 0.378
2.115AspGln: 2.115 ± 0.4
2.644AspArg: 2.644 ± 0.369
3.525AspSer: 3.525 ± 0.505
2.762AspThr: 2.762 ± 0.488
4.407AspVal: 4.407 ± 0.419
0.999AspTrp: 0.999 ± 0.21
3.702AspTyr: 3.702 ± 0.55
0.0AspXaa: 0.0 ± 0.0
Glu
4.877GluAla: 4.877 ± 0.719
1.528GluCys: 1.528 ± 0.304
2.585GluAsp: 2.585 ± 0.401
4.231GluGlu: 4.231 ± 0.512
2.82GluPhe: 2.82 ± 0.391
3.408GluGly: 3.408 ± 0.423
0.999GluHis: 0.999 ± 0.25
5.112GluIle: 5.112 ± 0.454
5.347GluLys: 5.347 ± 0.584
7.697GluLeu: 7.697 ± 0.797
2.233GluMet: 2.233 ± 0.371
3.114GluAsn: 3.114 ± 0.384
2.527GluPro: 2.527 ± 0.492
2.762GluGln: 2.762 ± 0.518
3.761GluArg: 3.761 ± 0.559
3.996GluSer: 3.996 ± 0.579
4.348GluThr: 4.348 ± 0.485
2.938GluVal: 2.938 ± 0.556
1.351GluTrp: 1.351 ± 0.31
2.82GluTyr: 2.82 ± 0.434
0.0GluXaa: 0.0 ± 0.0
Phe
1.998PheAla: 1.998 ± 0.477
0.353PheCys: 0.353 ± 0.123
2.644PheAsp: 2.644 ± 0.386
2.82PheGlu: 2.82 ± 0.407
0.823PhePhe: 0.823 ± 0.269
2.762PheGly: 2.762 ± 0.386
0.588PheHis: 0.588 ± 0.192
3.173PheIle: 3.173 ± 0.507
4.172PheLys: 4.172 ± 0.611
2.762PheLeu: 2.762 ± 0.469
0.94PheMet: 0.94 ± 0.187
2.997PheAsn: 2.997 ± 0.448
1.528PhePro: 1.528 ± 0.366
0.94PheGln: 0.94 ± 0.243
1.351PheArg: 1.351 ± 0.329
2.35PheSer: 2.35 ± 0.348
2.174PheThr: 2.174 ± 0.418
2.703PheVal: 2.703 ± 0.366
0.353PheTrp: 0.353 ± 0.14
1.586PheTyr: 1.586 ± 0.311
0.0PheXaa: 0.0 ± 0.0
Gly
3.525GlyAla: 3.525 ± 0.472
1.293GlyCys: 1.293 ± 0.3
5.171GlyAsp: 5.171 ± 0.569
5.171GlyGlu: 5.171 ± 0.53
3.996GlyPhe: 3.996 ± 0.574
3.467GlyGly: 3.467 ± 0.65
1.234GlyHis: 1.234 ± 0.273
3.702GlyIle: 3.702 ± 0.504
5.171GlyLys: 5.171 ± 0.489
4.524GlyLeu: 4.524 ± 0.5
1.469GlyMet: 1.469 ± 0.307
4.289GlyAsn: 4.289 ± 0.572
1.058GlyPro: 1.058 ± 0.217
2.174GlyGln: 2.174 ± 0.397
3.173GlyArg: 3.173 ± 0.385
3.761GlySer: 3.761 ± 0.706
3.408GlyThr: 3.408 ± 0.446
5.229GlyVal: 5.229 ± 0.548
1.234GlyTrp: 1.234 ± 0.285
2.174GlyTyr: 2.174 ± 0.42
0.0GlyXaa: 0.0 ± 0.0
His
0.764HisAla: 0.764 ± 0.224
0.294HisCys: 0.294 ± 0.143
1.528HisAsp: 1.528 ± 0.303
0.881HisGlu: 0.881 ± 0.322
0.823HisPhe: 0.823 ± 0.2
1.351HisGly: 1.351 ± 0.361
0.705HisHis: 0.705 ± 0.205
1.116HisIle: 1.116 ± 0.28
0.999HisLys: 0.999 ± 0.299
1.351HisLeu: 1.351 ± 0.286
0.294HisMet: 0.294 ± 0.121
0.881HisAsn: 0.881 ± 0.188
0.881HisPro: 0.881 ± 0.208
0.764HisGln: 0.764 ± 0.23
0.881HisArg: 0.881 ± 0.262
1.058HisSer: 1.058 ± 0.251
0.705HisThr: 0.705 ± 0.212
1.41HisVal: 1.41 ± 0.298
0.47HisTrp: 0.47 ± 0.148
0.529HisTyr: 0.529 ± 0.191
0.0HisXaa: 0.0 ± 0.0
Ile
5.876IleAla: 5.876 ± 0.652
0.881IleCys: 0.881 ± 0.24
5.758IleAsp: 5.758 ± 0.595
4.759IleGlu: 4.759 ± 0.476
1.704IlePhe: 1.704 ± 0.299
4.231IleGly: 4.231 ± 0.555
1.469IleHis: 1.469 ± 0.313
4.936IleIle: 4.936 ± 0.572
5.523IleLys: 5.523 ± 0.651
4.466IleLeu: 4.466 ± 0.503
1.763IleMet: 1.763 ± 0.342
4.583IleAsn: 4.583 ± 0.533
2.703IlePro: 2.703 ± 0.417
1.645IleGln: 1.645 ± 0.313
3.525IleArg: 3.525 ± 0.442
4.936IleSer: 4.936 ± 0.571
5.288IleThr: 5.288 ± 0.608
4.113IleVal: 4.113 ± 0.567
0.47IleTrp: 0.47 ± 0.146
2.703IleTyr: 2.703 ± 0.481
0.0IleXaa: 0.0 ± 0.0
Lys
5.053LysAla: 5.053 ± 0.656
1.058LysCys: 1.058 ± 0.274
3.819LysAsp: 3.819 ± 0.417
4.701LysGlu: 4.701 ± 0.58
3.114LysPhe: 3.114 ± 0.447
3.29LysGly: 3.29 ± 0.531
1.645LysHis: 1.645 ± 0.392
4.231LysIle: 4.231 ± 0.41
3.467LysLys: 3.467 ± 0.449
6.992LysLeu: 6.992 ± 0.737
3.29LysMet: 3.29 ± 0.452
3.525LysAsn: 3.525 ± 0.416
2.997LysPro: 2.997 ± 0.418
2.82LysGln: 2.82 ± 0.61
3.525LysArg: 3.525 ± 0.508
4.524LysSer: 4.524 ± 0.534
4.524LysThr: 4.524 ± 0.629
4.524LysVal: 4.524 ± 0.625
1.528LysTrp: 1.528 ± 0.331
3.349LysTyr: 3.349 ± 0.459
0.0LysXaa: 0.0 ± 0.0
Leu
5.229LeuAla: 5.229 ± 0.557
1.586LeuCys: 1.586 ± 0.339
4.994LeuAsp: 4.994 ± 0.448
6.757LeuGlu: 6.757 ± 0.585
2.527LeuPhe: 2.527 ± 0.324
3.996LeuGly: 3.996 ± 0.522
1.058LeuHis: 1.058 ± 0.252
5.817LeuIle: 5.817 ± 0.73
7.051LeuLys: 7.051 ± 0.745
5.347LeuLeu: 5.347 ± 0.678
2.762LeuMet: 2.762 ± 0.405
5.171LeuAsn: 5.171 ± 0.691
3.996LeuPro: 3.996 ± 0.444
2.35LeuGln: 2.35 ± 0.4
3.643LeuArg: 3.643 ± 0.503
4.348LeuSer: 4.348 ± 0.596
5.817LeuThr: 5.817 ± 0.704
4.701LeuVal: 4.701 ± 0.52
0.705LeuTrp: 0.705 ± 0.198
2.762LeuTyr: 2.762 ± 0.459
0.0LeuXaa: 0.0 ± 0.0
Met
1.645MetAla: 1.645 ± 0.307
0.294MetCys: 0.294 ± 0.128
1.234MetAsp: 1.234 ± 0.227
1.116MetGlu: 1.116 ± 0.221
0.999MetPhe: 0.999 ± 0.267
0.999MetGly: 0.999 ± 0.211
0.411MetHis: 0.411 ± 0.205
2.703MetIle: 2.703 ± 0.491
2.644MetLys: 2.644 ± 0.405
2.468MetLeu: 2.468 ± 0.334
1.351MetMet: 1.351 ± 0.286
1.88MetAsn: 1.88 ± 0.325
0.705MetPro: 0.705 ± 0.172
1.41MetGln: 1.41 ± 0.272
1.351MetArg: 1.351 ± 0.286
2.585MetSer: 2.585 ± 0.348
2.057MetThr: 2.057 ± 0.372
1.763MetVal: 1.763 ± 0.327
0.588MetTrp: 0.588 ± 0.169
0.881MetTyr: 0.881 ± 0.258
0.0MetXaa: 0.0 ± 0.0
Asn
3.819AsnAla: 3.819 ± 0.509
1.116AsnCys: 1.116 ± 0.258
3.29AsnAsp: 3.29 ± 0.412
3.467AsnGlu: 3.467 ± 0.526
1.88AsnPhe: 1.88 ± 0.3
5.464AsnGly: 5.464 ± 0.6
1.058AsnHis: 1.058 ± 0.252
5.464AsnIle: 5.464 ± 0.598
4.054AsnLys: 4.054 ± 0.554
4.113AsnLeu: 4.113 ± 0.488
1.234AsnMet: 1.234 ± 0.25
4.524AsnAsn: 4.524 ± 0.61
1.939AsnPro: 1.939 ± 0.375
2.35AsnGln: 2.35 ± 0.517
2.703AsnArg: 2.703 ± 0.417
2.938AsnSer: 2.938 ± 0.404
3.643AsnThr: 3.643 ± 0.53
3.761AsnVal: 3.761 ± 0.501
0.881AsnTrp: 0.881 ± 0.25
2.174AsnTyr: 2.174 ± 0.38
0.0AsnXaa: 0.0 ± 0.0
Pro
1.939ProAla: 1.939 ± 0.378
0.705ProCys: 0.705 ± 0.248
2.409ProAsp: 2.409 ± 0.447
2.82ProGlu: 2.82 ± 0.374
1.645ProPhe: 1.645 ± 0.342
2.057ProGly: 2.057 ± 0.299
0.646ProHis: 0.646 ± 0.192
2.35ProIle: 2.35 ± 0.392
1.763ProLys: 1.763 ± 0.425
2.115ProLeu: 2.115 ± 0.392
0.823ProMet: 0.823 ± 0.216
2.233ProAsn: 2.233 ± 0.396
0.823ProPro: 0.823 ± 0.29
0.94ProGln: 0.94 ± 0.242
1.821ProArg: 1.821 ± 0.284
2.292ProSer: 2.292 ± 0.302
2.174ProThr: 2.174 ± 0.352
3.055ProVal: 3.055 ± 0.628
0.411ProTrp: 0.411 ± 0.146
1.645ProTyr: 1.645 ± 0.308
0.0ProXaa: 0.0 ± 0.0
Gln
2.174GlnAla: 2.174 ± 0.536
0.529GlnCys: 0.529 ± 0.181
1.586GlnAsp: 1.586 ± 0.27
1.998GlnGlu: 1.998 ± 0.446
1.469GlnPhe: 1.469 ± 0.27
1.528GlnGly: 1.528 ± 0.3
0.353GlnHis: 0.353 ± 0.151
2.115GlnIle: 2.115 ± 0.331
1.704GlnLys: 1.704 ± 0.49
4.172GlnLeu: 4.172 ± 0.73
1.058GlnMet: 1.058 ± 0.245
1.528GlnAsn: 1.528 ± 0.346
1.293GlnPro: 1.293 ± 0.283
2.585GlnGln: 2.585 ± 0.897
1.704GlnArg: 1.704 ± 0.244
2.762GlnSer: 2.762 ± 0.462
1.704GlnThr: 1.704 ± 0.27
2.233GlnVal: 2.233 ± 0.383
0.764GlnTrp: 0.764 ± 0.168
1.234GlnTyr: 1.234 ± 0.33
0.0GlnXaa: 0.0 ± 0.0
Arg
3.349ArgAla: 3.349 ± 0.442
0.705ArgCys: 0.705 ± 0.201
3.232ArgAsp: 3.232 ± 0.439
2.703ArgGlu: 2.703 ± 0.416
1.88ArgPhe: 1.88 ± 0.289
3.29ArgGly: 3.29 ± 0.464
0.529ArgHis: 0.529 ± 0.164
3.173ArgIle: 3.173 ± 0.44
2.997ArgLys: 2.997 ± 0.388
4.407ArgLeu: 4.407 ± 0.462
0.705ArgMet: 0.705 ± 0.195
3.114ArgAsn: 3.114 ± 0.422
1.704ArgPro: 1.704 ± 0.305
1.586ArgGln: 1.586 ± 0.384
2.585ArgArg: 2.585 ± 0.346
2.468ArgSer: 2.468 ± 0.403
2.527ArgThr: 2.527 ± 0.391
3.467ArgVal: 3.467 ± 0.437
0.588ArgTrp: 0.588 ± 0.222
2.468ArgTyr: 2.468 ± 0.455
0.0ArgXaa: 0.0 ± 0.0
Ser
4.583SerAla: 4.583 ± 0.588
0.764SerCys: 0.764 ± 0.209
4.407SerAsp: 4.407 ± 0.494
4.054SerGlu: 4.054 ± 0.393
2.468SerPhe: 2.468 ± 0.4
4.172SerGly: 4.172 ± 0.549
1.234SerHis: 1.234 ± 0.295
3.878SerIle: 3.878 ± 0.403
4.642SerLys: 4.642 ± 0.568
4.994SerLeu: 4.994 ± 0.592
1.41SerMet: 1.41 ± 0.27
3.584SerAsn: 3.584 ± 0.498
1.704SerPro: 1.704 ± 0.304
2.174SerGln: 2.174 ± 0.434
2.703SerArg: 2.703 ± 0.414
3.643SerSer: 3.643 ± 0.421
3.232SerThr: 3.232 ± 0.44
4.936SerVal: 4.936 ± 0.428
0.999SerTrp: 0.999 ± 0.268
1.645SerTyr: 1.645 ± 0.34
0.0SerXaa: 0.0 ± 0.0
Thr
2.938ThrAla: 2.938 ± 0.439
0.705ThrCys: 0.705 ± 0.207
3.173ThrAsp: 3.173 ± 0.432
3.29ThrGlu: 3.29 ± 0.541
2.527ThrPhe: 2.527 ± 0.416
6.522ThrGly: 6.522 ± 0.603
1.41ThrHis: 1.41 ± 0.329
5.406ThrIle: 5.406 ± 0.577
3.408ThrLys: 3.408 ± 0.44
5.523ThrLeu: 5.523 ± 0.562
1.704ThrMet: 1.704 ± 0.336
2.174ThrAsn: 2.174 ± 0.333
2.762ThrPro: 2.762 ± 0.501
2.115ThrGln: 2.115 ± 0.466
2.585ThrArg: 2.585 ± 0.369
2.703ThrSer: 2.703 ± 0.381
2.468ThrThr: 2.468 ± 0.406
4.407ThrVal: 4.407 ± 0.682
0.823ThrTrp: 0.823 ± 0.202
1.88ThrTyr: 1.88 ± 0.33
0.0ThrXaa: 0.0 ± 0.0
Val
3.937ValAla: 3.937 ± 0.595
0.294ValCys: 0.294 ± 0.14
4.524ValAsp: 4.524 ± 0.466
4.466ValGlu: 4.466 ± 0.431
2.82ValPhe: 2.82 ± 0.447
4.818ValGly: 4.818 ± 0.519
0.764ValHis: 0.764 ± 0.231
5.464ValIle: 5.464 ± 0.486
4.936ValLys: 4.936 ± 0.618
4.466ValLeu: 4.466 ± 0.433
1.234ValMet: 1.234 ± 0.25
4.407ValAsn: 4.407 ± 0.483
1.998ValPro: 1.998 ± 0.281
1.234ValGln: 1.234 ± 0.245
3.173ValArg: 3.173 ± 0.434
5.171ValSer: 5.171 ± 0.558
4.877ValThr: 4.877 ± 0.736
2.527ValVal: 2.527 ± 0.399
0.881ValTrp: 0.881 ± 0.216
3.055ValTyr: 3.055 ± 0.461
0.0ValXaa: 0.0 ± 0.0
Trp
0.823TrpAla: 0.823 ± 0.214
0.411TrpCys: 0.411 ± 0.154
1.469TrpAsp: 1.469 ± 0.301
1.175TrpGlu: 1.175 ± 0.256
0.764TrpPhe: 0.764 ± 0.263
0.764TrpGly: 0.764 ± 0.253
0.235TrpHis: 0.235 ± 0.107
0.705TrpIle: 0.705 ± 0.216
0.94TrpLys: 0.94 ± 0.224
1.469TrpLeu: 1.469 ± 0.311
0.235TrpMet: 0.235 ± 0.12
0.823TrpAsn: 0.823 ± 0.19
0.0TrpPro: 0.0 ± 0.0
0.235TrpGln: 0.235 ± 0.114
0.823TrpArg: 0.823 ± 0.217
0.881TrpSer: 0.881 ± 0.192
0.705TrpThr: 0.705 ± 0.239
1.116TrpVal: 1.116 ± 0.241
0.47TrpTrp: 0.47 ± 0.177
0.764TrpTyr: 0.764 ± 0.211
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.409TyrAla: 2.409 ± 0.378
0.764TyrCys: 0.764 ± 0.213
2.762TyrAsp: 2.762 ± 0.39
3.408TyrGlu: 3.408 ± 0.479
1.88TyrPhe: 1.88 ± 0.32
2.527TyrGly: 2.527 ± 0.354
0.94TyrHis: 0.94 ± 0.224
2.292TyrIle: 2.292 ± 0.347
2.292TyrLys: 2.292 ± 0.393
2.703TyrLeu: 2.703 ± 0.481
0.94TyrMet: 0.94 ± 0.215
2.174TyrAsn: 2.174 ± 0.412
1.234TyrPro: 1.234 ± 0.354
1.645TyrGln: 1.645 ± 0.342
2.409TyrArg: 2.409 ± 0.332
2.644TyrSer: 2.644 ± 0.417
2.115TyrThr: 2.115 ± 0.281
2.35TyrVal: 2.35 ± 0.421
0.411TyrTrp: 0.411 ± 0.143
1.645TyrTyr: 1.645 ± 0.314
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (17020 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski