Microbacterium phage Pabst

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Eekayvirinae; Tinytimothyvirus; unclassified Tinytimothyvirus

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3VB39|A0A7G3VB39_9CAUD Uncharacterized protein OS=Microbacterium phage Pabst OX=2743923 GN=30 PE=4 SV=1
MM1 pKa = 7.72SEE3 pKa = 4.65ALNKK7 pKa = 9.54CTDD10 pKa = 3.67TVVDD14 pKa = 3.46NCFVQFRR21 pKa = 11.84DD22 pKa = 3.67TVTVHH27 pKa = 6.33YY28 pKa = 10.01KK29 pKa = 11.11GEE31 pKa = 4.25DD32 pKa = 3.19FWFSVPDD39 pKa = 3.81GANAIYY45 pKa = 10.24AIGEE49 pKa = 4.4DD50 pKa = 3.92CTDD53 pKa = 4.31AYY55 pKa = 10.49AACNTAGVVWEE66 pKa = 4.89FEE68 pKa = 4.16PVLQQSTLPATGLDD82 pKa = 3.56PTGLIIGVVVGVLLAGLGAWAVSIGASRR110 pKa = 11.84HH111 pKa = 4.67NKK113 pKa = 9.69RR114 pKa = 11.84KK115 pKa = 10.46DD116 pKa = 3.13GG117 pKa = 3.83

Molecular weight: 12.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3VAZ5|A0A7G3VAZ5_9CAUD DNA helicase OS=Microbacterium phage Pabst OX=2743923 GN=5 PE=4 SV=1
MM1 pKa = 7.14VLNRR5 pKa = 11.84HH6 pKa = 4.76KK7 pKa = 9.52TVRR10 pKa = 11.84QALSYY15 pKa = 10.14VDD17 pKa = 5.71RR18 pKa = 11.84YY19 pKa = 10.19PEE21 pKa = 3.71WPAIDD26 pKa = 5.24RR27 pKa = 11.84IDD29 pKa = 3.55MPIWEE34 pKa = 4.4LVARR38 pKa = 11.84NLFDD42 pKa = 3.82IANHH46 pKa = 5.73PNVRR50 pKa = 11.84VRR52 pKa = 11.84GSVAKK57 pKa = 9.25ATRR60 pKa = 11.84AQRR63 pKa = 11.84IILDD67 pKa = 3.69RR68 pKa = 11.84LTGTRR73 pKa = 11.84RR74 pKa = 11.84QGTNPAVQNTKK85 pKa = 9.72RR86 pKa = 11.84VQLLDD91 pKa = 3.72LTVSLPTPTEE101 pKa = 4.25GPQNDD106 pKa = 3.99GSDD109 pKa = 3.31QRR111 pKa = 11.84RR112 pKa = 11.84EE113 pKa = 4.03AEE115 pKa = 4.0EE116 pKa = 3.76

Molecular weight: 13.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

16773

39

4486

305.0

33.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.682 ± 0.398

0.393 ± 0.136

6.54 ± 0.248

6.481 ± 0.333

3.44 ± 0.134

7.625 ± 0.333

1.55 ± 0.185

4.972 ± 0.16

4.436 ± 0.355

8.484 ± 0.408

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.653 ± 0.165

4.805 ± 0.281

4.728 ± 0.252

4.466 ± 0.235

6.063 ± 0.285

6.153 ± 0.269

6.409 ± 0.324

6.224 ± 0.25

1.711 ± 0.125

3.184 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski