Amino acid dipepetide frequency for Microbacterium phage Pabst

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.925AlaAla: 11.925 ± 1.295
0.358AlaCys: 0.358 ± 0.198
5.843AlaAsp: 5.843 ± 0.474
6.022AlaGlu: 6.022 ± 0.707
3.458AlaPhe: 3.458 ± 0.438
6.857AlaGly: 6.857 ± 0.672
1.491AlaHis: 1.491 ± 0.409
4.889AlaIle: 4.889 ± 0.542
3.935AlaLys: 3.935 ± 0.638
8.109AlaLeu: 8.109 ± 0.58
2.623AlaMet: 2.623 ± 0.577
4.293AlaAsn: 4.293 ± 0.493
5.247AlaPro: 5.247 ± 0.86
3.876AlaGln: 3.876 ± 0.441
5.962AlaArg: 5.962 ± 0.742
5.962AlaSer: 5.962 ± 0.672
5.306AlaThr: 5.306 ± 0.455
5.605AlaVal: 5.605 ± 0.602
1.789AlaTrp: 1.789 ± 0.279
3.279AlaTyr: 3.279 ± 0.366
0.0AlaXaa: 0.0 ± 0.0
Cys
0.179CysAla: 0.179 ± 0.111
0.0CysCys: 0.0 ± 0.0
0.179CysAsp: 0.179 ± 0.12
0.238CysGlu: 0.238 ± 0.162
0.238CysPhe: 0.238 ± 0.157
0.417CysGly: 0.417 ± 0.195
0.06CysHis: 0.06 ± 0.065
0.298CysIle: 0.298 ± 0.157
0.119CysLys: 0.119 ± 0.08
0.417CysLeu: 0.417 ± 0.209
0.179CysMet: 0.179 ± 0.105
0.119CysAsn: 0.119 ± 0.097
0.238CysPro: 0.238 ± 0.209
0.0CysGln: 0.0 ± 0.0
0.06CysArg: 0.06 ± 0.058
0.358CysSer: 0.358 ± 0.172
0.358CysThr: 0.358 ± 0.232
0.298CysVal: 0.298 ± 0.179
0.06CysTrp: 0.06 ± 0.083
0.119CysTyr: 0.119 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
6.857AspAla: 6.857 ± 0.834
0.358AspCys: 0.358 ± 0.177
4.054AspAsp: 4.054 ± 0.484
5.068AspGlu: 5.068 ± 0.6
2.504AspPhe: 2.504 ± 0.31
6.141AspGly: 6.141 ± 0.545
1.014AspHis: 1.014 ± 0.232
3.518AspIle: 3.518 ± 0.381
2.922AspLys: 2.922 ± 0.6
5.545AspLeu: 5.545 ± 0.633
2.027AspMet: 2.027 ± 0.369
2.862AspAsn: 2.862 ± 0.354
2.981AspPro: 2.981 ± 0.39
2.922AspGln: 2.922 ± 0.35
3.399AspArg: 3.399 ± 0.467
3.518AspSer: 3.518 ± 0.374
3.279AspThr: 3.279 ± 0.412
3.399AspVal: 3.399 ± 0.602
1.133AspTrp: 1.133 ± 0.235
1.908AspTyr: 1.908 ± 0.282
0.0AspXaa: 0.0 ± 0.0
Glu
6.559GluAla: 6.559 ± 0.603
0.06GluCys: 0.06 ± 0.082
4.114GluAsp: 4.114 ± 0.468
3.697GluGlu: 3.697 ± 0.595
2.206GluPhe: 2.206 ± 0.495
4.949GluGly: 4.949 ± 0.514
1.252GluHis: 1.252 ± 0.239
3.756GluIle: 3.756 ± 0.533
3.339GluLys: 3.339 ± 0.5
5.724GluLeu: 5.724 ± 0.685
1.312GluMet: 1.312 ± 0.255
3.279GluAsn: 3.279 ± 0.58
2.146GluPro: 2.146 ± 0.524
3.041GluGln: 3.041 ± 0.464
4.233GluArg: 4.233 ± 0.563
3.697GluSer: 3.697 ± 0.407
3.518GluThr: 3.518 ± 0.487
3.935GluVal: 3.935 ± 0.427
1.252GluTrp: 1.252 ± 0.383
2.743GluTyr: 2.743 ± 0.277
0.0GluXaa: 0.0 ± 0.0
Phe
2.146PheAla: 2.146 ± 0.352
0.417PheCys: 0.417 ± 0.211
2.385PheAsp: 2.385 ± 0.391
2.862PheGlu: 2.862 ± 0.496
1.192PhePhe: 1.192 ± 0.26
3.458PheGly: 3.458 ± 0.489
0.417PheHis: 0.417 ± 0.192
1.908PheIle: 1.908 ± 0.349
1.252PheLys: 1.252 ± 0.198
2.981PheLeu: 2.981 ± 0.34
0.835PheMet: 0.835 ± 0.248
1.431PheAsn: 1.431 ± 0.244
1.55PhePro: 1.55 ± 0.271
1.192PheGln: 1.192 ± 0.363
2.922PheArg: 2.922 ± 0.429
1.968PheSer: 1.968 ± 0.283
2.146PheThr: 2.146 ± 0.438
2.206PheVal: 2.206 ± 0.298
0.417PheTrp: 0.417 ± 0.134
0.715PheTyr: 0.715 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
6.976GlyAla: 6.976 ± 0.788
0.119GlyCys: 0.119 ± 0.096
5.008GlyAsp: 5.008 ± 0.53
4.233GlyGlu: 4.233 ± 0.454
3.22GlyPhe: 3.22 ± 0.398
7.751GlyGly: 7.751 ± 1.188
0.835GlyHis: 0.835 ± 0.2
4.114GlyIle: 4.114 ± 0.446
3.518GlyLys: 3.518 ± 0.473
5.783GlyLeu: 5.783 ± 0.536
2.266GlyMet: 2.266 ± 0.332
3.279GlyAsn: 3.279 ± 0.541
2.623GlyPro: 2.623 ± 0.456
2.802GlyGln: 2.802 ± 0.324
4.352GlyArg: 4.352 ± 0.724
4.293GlySer: 4.293 ± 0.485
5.664GlyThr: 5.664 ± 0.56
5.247GlyVal: 5.247 ± 0.779
1.192GlyTrp: 1.192 ± 0.284
2.981GlyTyr: 2.981 ± 0.39
0.0GlyXaa: 0.0 ± 0.0
His
0.894HisAla: 0.894 ± 0.195
0.06HisCys: 0.06 ± 0.065
1.491HisAsp: 1.491 ± 0.472
1.133HisGlu: 1.133 ± 0.282
0.358HisPhe: 0.358 ± 0.134
1.014HisGly: 1.014 ± 0.233
0.358HisHis: 0.358 ± 0.175
0.656HisIle: 0.656 ± 0.221
1.252HisLys: 1.252 ± 0.382
1.252HisLeu: 1.252 ± 0.241
0.238HisMet: 0.238 ± 0.134
0.477HisAsn: 0.477 ± 0.133
1.073HisPro: 1.073 ± 0.192
0.775HisGln: 0.775 ± 0.218
1.252HisArg: 1.252 ± 0.251
0.477HisSer: 0.477 ± 0.125
0.894HisThr: 0.894 ± 0.317
1.192HisVal: 1.192 ± 0.268
0.238HisTrp: 0.238 ± 0.118
0.417HisTyr: 0.417 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
4.472IleAla: 4.472 ± 0.764
0.119IleCys: 0.119 ± 0.083
4.054IleAsp: 4.054 ± 0.562
3.637IleGlu: 3.637 ± 0.389
1.491IlePhe: 1.491 ± 0.299
2.862IleGly: 2.862 ± 0.548
0.477IleHis: 0.477 ± 0.2
2.504IleIle: 2.504 ± 0.426
2.445IleLys: 2.445 ± 0.422
3.637IleLeu: 3.637 ± 0.496
0.775IleMet: 0.775 ± 0.201
3.16IleAsn: 3.16 ± 0.376
2.087IlePro: 2.087 ± 0.411
2.445IleGln: 2.445 ± 0.388
3.16IleArg: 3.16 ± 0.362
3.399IleSer: 3.399 ± 0.395
3.399IleThr: 3.399 ± 0.455
3.1IleVal: 3.1 ± 0.366
0.894IleTrp: 0.894 ± 0.204
1.55IleTyr: 1.55 ± 0.266
0.0IleXaa: 0.0 ± 0.0
Lys
3.756LysAla: 3.756 ± 0.528
0.179LysCys: 0.179 ± 0.122
2.922LysAsp: 2.922 ± 0.391
3.16LysGlu: 3.16 ± 0.461
1.073LysPhe: 1.073 ± 0.278
2.564LysGly: 2.564 ± 0.35
0.417LysHis: 0.417 ± 0.169
1.908LysIle: 1.908 ± 0.453
1.848LysLys: 1.848 ± 0.433
4.114LysLeu: 4.114 ± 0.643
1.491LysMet: 1.491 ± 0.408
2.862LysAsn: 2.862 ± 0.418
2.623LysPro: 2.623 ± 0.5
1.669LysGln: 1.669 ± 0.384
2.802LysArg: 2.802 ± 0.58
2.266LysSer: 2.266 ± 0.355
3.577LysThr: 3.577 ± 0.524
2.266LysVal: 2.266 ± 0.568
0.835LysTrp: 0.835 ± 0.227
2.027LysTyr: 2.027 ± 0.328
0.0LysXaa: 0.0 ± 0.0
Leu
7.93LeuAla: 7.93 ± 1.044
0.715LeuCys: 0.715 ± 0.354
5.903LeuAsp: 5.903 ± 0.753
5.426LeuGlu: 5.426 ± 0.583
2.802LeuPhe: 2.802 ± 0.423
6.201LeuGly: 6.201 ± 0.829
1.61LeuHis: 1.61 ± 0.23
3.935LeuIle: 3.935 ± 0.583
3.339LeuLys: 3.339 ± 0.652
7.393LeuLeu: 7.393 ± 0.914
3.22LeuMet: 3.22 ± 0.614
3.458LeuAsn: 3.458 ± 0.453
3.637LeuPro: 3.637 ± 0.566
4.591LeuGln: 4.591 ± 0.625
6.32LeuArg: 6.32 ± 0.574
5.485LeuSer: 5.485 ± 0.785
5.128LeuThr: 5.128 ± 0.622
4.233LeuVal: 4.233 ± 0.58
1.252LeuTrp: 1.252 ± 0.344
2.266LeuTyr: 2.266 ± 0.293
0.0LeuXaa: 0.0 ± 0.0
Met
2.862MetAla: 2.862 ± 0.404
0.06MetCys: 0.06 ± 0.064
1.371MetAsp: 1.371 ± 0.313
1.252MetGlu: 1.252 ± 0.299
1.61MetPhe: 1.61 ± 0.242
1.908MetGly: 1.908 ± 0.489
0.477MetHis: 0.477 ± 0.171
1.252MetIle: 1.252 ± 0.28
1.192MetLys: 1.192 ± 0.215
2.385MetLeu: 2.385 ± 0.361
0.715MetMet: 0.715 ± 0.199
1.252MetAsn: 1.252 ± 0.229
1.669MetPro: 1.669 ± 0.316
1.491MetGln: 1.491 ± 0.334
0.894MetArg: 0.894 ± 0.298
2.087MetSer: 2.087 ± 0.312
1.491MetThr: 1.491 ± 0.362
1.371MetVal: 1.371 ± 0.314
0.358MetTrp: 0.358 ± 0.156
0.835MetTyr: 0.835 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
5.306AsnAla: 5.306 ± 0.714
0.358AsnCys: 0.358 ± 0.167
2.743AsnAsp: 2.743 ± 0.358
2.802AsnGlu: 2.802 ± 0.296
1.192AsnPhe: 1.192 ± 0.241
4.114AsnGly: 4.114 ± 0.353
0.656AsnHis: 0.656 ± 0.177
2.445AsnIle: 2.445 ± 0.496
2.981AsnLys: 2.981 ± 0.481
3.041AsnLeu: 3.041 ± 0.478
1.133AsnMet: 1.133 ± 0.313
2.385AsnAsn: 2.385 ± 0.487
3.339AsnPro: 3.339 ± 0.583
1.729AsnGln: 1.729 ± 0.299
1.789AsnArg: 1.789 ± 0.325
2.385AsnSer: 2.385 ± 0.421
3.935AsnThr: 3.935 ± 0.503
3.577AsnVal: 3.577 ± 0.521
0.656AsnTrp: 0.656 ± 0.166
1.491AsnTyr: 1.491 ± 0.209
0.0AsnXaa: 0.0 ± 0.0
Pro
4.71ProAla: 4.71 ± 0.554
0.06ProCys: 0.06 ± 0.069
2.802ProAsp: 2.802 ± 0.388
4.054ProGlu: 4.054 ± 0.485
1.61ProPhe: 1.61 ± 0.285
3.22ProGly: 3.22 ± 0.501
0.477ProHis: 0.477 ± 0.222
2.206ProIle: 2.206 ± 0.371
1.968ProLys: 1.968 ± 0.409
3.756ProLeu: 3.756 ± 0.328
0.715ProMet: 0.715 ± 0.255
2.981ProAsn: 2.981 ± 0.371
1.61ProPro: 1.61 ± 0.331
1.431ProGln: 1.431 ± 0.342
2.206ProArg: 2.206 ± 0.466
2.862ProSer: 2.862 ± 0.392
3.876ProThr: 3.876 ± 0.536
4.591ProVal: 4.591 ± 0.496
0.835ProTrp: 0.835 ± 0.206
1.312ProTyr: 1.312 ± 0.221
0.0ProXaa: 0.0 ± 0.0
Gln
5.664GlnAla: 5.664 ± 0.464
0.179GlnCys: 0.179 ± 0.115
2.504GlnAsp: 2.504 ± 0.331
2.683GlnGlu: 2.683 ± 0.393
1.192GlnPhe: 1.192 ± 0.254
2.564GlnGly: 2.564 ± 0.437
0.954GlnHis: 0.954 ± 0.178
2.683GlnIle: 2.683 ± 0.368
1.908GlnLys: 1.908 ± 0.397
4.591GlnLeu: 4.591 ± 0.501
1.312GlnMet: 1.312 ± 0.282
2.146GlnAsn: 2.146 ± 0.456
1.491GlnPro: 1.491 ± 0.202
2.266GlnGln: 2.266 ± 0.437
2.922GlnArg: 2.922 ± 0.506
2.981GlnSer: 2.981 ± 0.37
2.087GlnThr: 2.087 ± 0.294
2.325GlnVal: 2.325 ± 0.442
0.894GlnTrp: 0.894 ± 0.214
1.312GlnTyr: 1.312 ± 0.226
0.0GlnXaa: 0.0 ± 0.0
Arg
4.472ArgAla: 4.472 ± 0.799
0.06ArgCys: 0.06 ± 0.078
3.697ArgAsp: 3.697 ± 0.451
4.591ArgGlu: 4.591 ± 0.59
2.146ArgPhe: 2.146 ± 0.492
4.889ArgGly: 4.889 ± 0.534
0.715ArgHis: 0.715 ± 0.286
2.862ArgIle: 2.862 ± 0.379
2.743ArgLys: 2.743 ± 0.497
5.247ArgLeu: 5.247 ± 0.637
1.431ArgMet: 1.431 ± 0.241
2.981ArgAsn: 2.981 ± 0.445
2.922ArgPro: 2.922 ± 0.415
3.16ArgGln: 3.16 ± 0.388
4.829ArgArg: 4.829 ± 0.871
3.458ArgSer: 3.458 ± 0.507
3.876ArgThr: 3.876 ± 0.505
4.293ArgVal: 4.293 ± 0.609
0.537ArgTrp: 0.537 ± 0.166
1.729ArgTyr: 1.729 ± 0.344
0.0ArgXaa: 0.0 ± 0.0
Ser
5.366SerAla: 5.366 ± 0.453
0.119SerCys: 0.119 ± 0.084
3.935SerAsp: 3.935 ± 0.428
3.339SerGlu: 3.339 ± 0.43
2.922SerPhe: 2.922 ± 0.405
5.247SerGly: 5.247 ± 0.77
1.192SerHis: 1.192 ± 0.296
2.683SerIle: 2.683 ± 0.409
2.206SerLys: 2.206 ± 0.394
5.724SerLeu: 5.724 ± 0.684
1.61SerMet: 1.61 ± 0.257
3.577SerAsn: 3.577 ± 0.478
2.504SerPro: 2.504 ± 0.381
2.504SerGln: 2.504 ± 0.342
3.279SerArg: 3.279 ± 0.386
3.876SerSer: 3.876 ± 0.595
4.352SerThr: 4.352 ± 0.589
3.876SerVal: 3.876 ± 0.437
1.431SerTrp: 1.431 ± 0.252
1.789SerTyr: 1.789 ± 0.324
0.0SerXaa: 0.0 ± 0.0
Thr
6.38ThrAla: 6.38 ± 0.655
0.119ThrCys: 0.119 ± 0.093
3.995ThrAsp: 3.995 ± 0.411
4.352ThrGlu: 4.352 ± 0.365
1.669ThrPhe: 1.669 ± 0.391
5.485ThrGly: 5.485 ± 0.703
0.954ThrHis: 0.954 ± 0.332
2.683ThrIle: 2.683 ± 0.426
2.087ThrLys: 2.087 ± 0.346
6.141ThrLeu: 6.141 ± 0.616
1.789ThrMet: 1.789 ± 0.309
2.504ThrAsn: 2.504 ± 0.418
3.577ThrPro: 3.577 ± 0.419
2.683ThrGln: 2.683 ± 0.416
3.876ThrArg: 3.876 ± 0.479
4.352ThrSer: 4.352 ± 0.518
4.77ThrThr: 4.77 ± 0.636
3.458ThrVal: 3.458 ± 0.553
1.371ThrTrp: 1.371 ± 0.299
1.848ThrTyr: 1.848 ± 0.431
0.0ThrXaa: 0.0 ± 0.0
Val
5.783ValAla: 5.783 ± 0.451
0.238ValCys: 0.238 ± 0.144
4.77ValAsp: 4.77 ± 0.58
3.458ValGlu: 3.458 ± 0.531
1.61ValPhe: 1.61 ± 0.287
3.876ValGly: 3.876 ± 0.405
1.312ValHis: 1.312 ± 0.287
3.279ValIle: 3.279 ± 0.402
3.16ValLys: 3.16 ± 0.439
5.187ValLeu: 5.187 ± 0.518
1.431ValMet: 1.431 ± 0.24
1.968ValAsn: 1.968 ± 0.351
4.233ValPro: 4.233 ± 0.611
3.458ValGln: 3.458 ± 0.405
3.876ValArg: 3.876 ± 0.535
4.233ValSer: 4.233 ± 0.518
3.279ValThr: 3.279 ± 0.42
3.995ValVal: 3.995 ± 0.741
1.252ValTrp: 1.252 ± 0.412
1.848ValTyr: 1.848 ± 0.356
0.0ValXaa: 0.0 ± 0.0
Trp
1.431TrpAla: 1.431 ± 0.304
0.06TrpCys: 0.06 ± 0.078
1.55TrpAsp: 1.55 ± 0.287
0.715TrpGlu: 0.715 ± 0.246
0.477TrpPhe: 0.477 ± 0.193
1.133TrpGly: 1.133 ± 0.257
0.298TrpHis: 0.298 ± 0.132
0.656TrpIle: 0.656 ± 0.172
0.775TrpLys: 0.775 ± 0.275
1.371TrpLeu: 1.371 ± 0.271
0.238TrpMet: 0.238 ± 0.136
1.073TrpAsn: 1.073 ± 0.237
0.417TrpPro: 0.417 ± 0.144
1.014TrpGln: 1.014 ± 0.294
0.715TrpArg: 0.715 ± 0.213
1.789TrpSer: 1.789 ± 0.37
1.192TrpThr: 1.192 ± 0.251
1.252TrpVal: 1.252 ± 0.219
0.358TrpTrp: 0.358 ± 0.206
0.596TrpTyr: 0.596 ± 0.243
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.16TyrAla: 3.16 ± 0.389
0.298TyrCys: 0.298 ± 0.149
2.087TyrAsp: 2.087 ± 0.371
2.087TyrGlu: 2.087 ± 0.36
1.431TyrPhe: 1.431 ± 0.405
1.61TyrGly: 1.61 ± 0.308
0.537TyrHis: 0.537 ± 0.211
1.491TyrIle: 1.491 ± 0.263
1.371TyrLys: 1.371 ± 0.405
2.445TyrLeu: 2.445 ± 0.396
1.133TyrMet: 1.133 ± 0.219
1.968TyrAsn: 1.968 ± 0.393
1.312TyrPro: 1.312 ± 0.236
1.61TyrGln: 1.61 ± 0.262
1.789TyrArg: 1.789 ± 0.321
2.087TyrSer: 2.087 ± 0.328
1.968TyrThr: 1.968 ± 0.324
2.027TyrVal: 2.027 ± 0.369
0.358TyrTrp: 0.358 ± 0.132
1.073TyrTyr: 1.073 ± 0.231
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (16773 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski