Nitrincola nitratireducens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Nitrincola

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4019 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W9V0J3|W9V0J3_9GAMM Lipid A biosynthesis lauroyl acyltransferase OS=Nitrincola nitratireducens OX=1229521 GN=D791_00176 PE=4 SV=1
MM1 pKa = 7.26RR2 pKa = 11.84TKK4 pKa = 10.93LLLPLILSCLLALPALAADD23 pKa = 4.19YY24 pKa = 11.13YY25 pKa = 11.82VDD27 pKa = 3.39ITNKK31 pKa = 9.24TGYY34 pKa = 9.92QINQLYY40 pKa = 10.09VSPGDD45 pKa = 3.47QKK47 pKa = 11.24SWEE50 pKa = 4.1EE51 pKa = 3.91DD52 pKa = 3.34VLGNDD57 pKa = 3.4VLVNGTTQRR66 pKa = 11.84VTLTGYY72 pKa = 10.09SSPVFDD78 pKa = 4.78IRR80 pKa = 11.84LIDD83 pKa = 3.83SDD85 pKa = 3.8GDD87 pKa = 4.22SYY89 pKa = 10.77TFWDD93 pKa = 3.99INVEE97 pKa = 4.11EE98 pKa = 4.58YY99 pKa = 11.14DD100 pKa = 4.75LLVTPDD106 pKa = 4.62DD107 pKa = 4.38LDD109 pKa = 3.47

Molecular weight: 12.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W9V250|W9V250_9GAMM D-malate degradation protein R OS=Nitrincola nitratireducens OX=1229521 GN=dmlR_6 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84QVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.89SLTVV44 pKa = 3.12

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4019

0

4019

1211048

30

2529

301.3

33.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.039 ± 0.045

1.048 ± 0.014

5.401 ± 0.032

6.129 ± 0.04

4.005 ± 0.027

6.81 ± 0.036

2.401 ± 0.02

6.115 ± 0.037

4.217 ± 0.035

11.317 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.647 ± 0.017

3.608 ± 0.027

4.278 ± 0.026

4.675 ± 0.028

5.555 ± 0.032

6.613 ± 0.034

5.157 ± 0.025

6.913 ± 0.035

1.312 ± 0.017

2.76 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski