Hubei diptera virus 15

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGG9|A0A1L3KGG9_9VIRU RNA-directed RNA polymerase OS=Hubei diptera virus 15 OX=1922876 PE=4 SV=1
MM1 pKa = 7.79NIRR4 pKa = 11.84EE5 pKa = 4.22VYY7 pKa = 10.39NYY9 pKa = 10.85LFGEE13 pKa = 5.42GSAQDD18 pKa = 3.41QTEE21 pKa = 4.08DD22 pKa = 3.39EE23 pKa = 4.23LRR25 pKa = 11.84KK26 pKa = 10.15QIEE29 pKa = 4.32SLAKK33 pKa = 10.49VLEE36 pKa = 4.08QSHH39 pKa = 6.61KK40 pKa = 10.37IKK42 pKa = 10.73EE43 pKa = 4.16LMAQQPQPGDD53 pKa = 3.38TPTKK57 pKa = 9.85TKK59 pKa = 11.1EE60 pKa = 3.64EE61 pKa = 4.2LNYY64 pKa = 10.03VRR66 pKa = 11.84AKK68 pKa = 9.61ATAEE72 pKa = 4.15TLEE75 pKa = 4.29HH76 pKa = 6.62LAVIDD81 pKa = 5.24DD82 pKa = 3.91KK83 pKa = 11.09TLPPSVEE90 pKa = 4.36DD91 pKa = 3.84GLAFLKK97 pKa = 9.52TRR99 pKa = 11.84CEE101 pKa = 4.19GKK103 pKa = 10.45KK104 pKa = 10.05VDD106 pKa = 3.59HH107 pKa = 6.99TYY109 pKa = 11.05NAYY112 pKa = 9.65CASIVRR118 pKa = 11.84QYY120 pKa = 11.42LQTRR124 pKa = 11.84KK125 pKa = 8.5KK126 pKa = 8.74TSNKK130 pKa = 8.74MEE132 pKa = 4.11YY133 pKa = 10.53AIMNSIMNIHH143 pKa = 7.52LINLRR148 pKa = 11.84PQYY151 pKa = 10.94DD152 pKa = 3.78YY153 pKa = 11.65DD154 pKa = 3.29LHH156 pKa = 8.32EE157 pKa = 4.26IMKK160 pKa = 10.92YY161 pKa = 9.99NDD163 pKa = 4.22CINYY167 pKa = 7.61VQRR170 pKa = 11.84KK171 pKa = 5.02TFRR174 pKa = 11.84LFNSIYY180 pKa = 9.67EE181 pKa = 4.17IPFTHH186 pKa = 7.29INLEE190 pKa = 4.13PLNITALSLWNALPKK205 pKa = 10.15PLSKK209 pKa = 10.39FLQVLLLIITTYY221 pKa = 10.41FVVSGIILPSIIHH234 pKa = 6.33LTTSTTHH241 pKa = 5.6SQPPPPLQPMPIVTDD256 pKa = 3.3QSGWMSTTAHH266 pKa = 6.52YY267 pKa = 7.66LTTTACYY274 pKa = 9.84AGSSVAYY281 pKa = 9.9SLGAMTSTLSRR292 pKa = 11.84WIAPASASSTWIPSWIWKK310 pKa = 9.3PEE312 pKa = 3.91EE313 pKa = 3.98PSLIEE318 pKa = 4.12SLHH321 pKa = 5.49SQSSKK326 pKa = 9.36RR327 pKa = 11.84WEE329 pKa = 4.07QISTKK334 pKa = 10.6LHH336 pKa = 6.73DD337 pKa = 4.79LFKK340 pKa = 10.88LATPPLTWLLDD351 pKa = 3.53VTSNHH356 pKa = 6.06LNALSSSAIISAKK369 pKa = 10.55EE370 pKa = 3.92LMTKK374 pKa = 10.37LAPKK378 pKa = 8.51WHH380 pKa = 6.82ASVEE384 pKa = 4.68SINTTLRR391 pKa = 11.84EE392 pKa = 4.07TILPSMHH399 pKa = 5.96TSLWKK404 pKa = 10.63CYY406 pKa = 10.52DD407 pKa = 3.4YY408 pKa = 10.99ATDD411 pKa = 4.62FIYY414 pKa = 10.39GASTTTASYY423 pKa = 10.17YY424 pKa = 10.89DD425 pKa = 4.18SVVKK429 pKa = 9.95PYY431 pKa = 9.64ATEE434 pKa = 4.96LYY436 pKa = 10.17HH437 pKa = 8.26GMVNATPSMARR448 pKa = 11.84ACQVTLTRR456 pKa = 11.84VLVTVLSITTSLGLFSLTCISVAMQLSMEE485 pKa = 4.84TITSFLQTYY494 pKa = 9.25QLMAVQQQPHH504 pKa = 6.38SDD506 pKa = 3.14VTTWIQSFFHH516 pKa = 5.43QVVIFTQLAFVVLSYY531 pKa = 8.81FTTRR535 pKa = 11.84TVIQRR540 pKa = 11.84WDD542 pKa = 4.4LIPYY546 pKa = 9.31DD547 pKa = 3.81SRR549 pKa = 11.84RR550 pKa = 11.84SMVALTT556 pKa = 3.92

Molecular weight: 63.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGG9|A0A1L3KGG9_9VIRU RNA-directed RNA polymerase OS=Hubei diptera virus 15 OX=1922876 PE=4 SV=1
MM1 pKa = 7.57LRR3 pKa = 11.84RR4 pKa = 11.84FKK6 pKa = 11.03CRR8 pKa = 11.84VQPWSYY14 pKa = 11.58DD15 pKa = 3.47EE16 pKa = 5.22YY17 pKa = 10.58IEE19 pKa = 6.26SMDD22 pKa = 4.41CPSKK26 pKa = 10.59RR27 pKa = 11.84QFYY30 pKa = 10.91LDD32 pKa = 3.44TKK34 pKa = 11.21LDD36 pKa = 3.68MEE38 pKa = 4.91AGRR41 pKa = 11.84TISNRR46 pKa = 11.84ITPFTKK52 pKa = 10.27LEE54 pKa = 3.93KK55 pKa = 10.2MGTNKK60 pKa = 10.03YY61 pKa = 9.36KK62 pKa = 10.66APRR65 pKa = 11.84LIQARR70 pKa = 11.84HH71 pKa = 4.84PTFNLALGCYY81 pKa = 9.44IKK83 pKa = 10.61PLEE86 pKa = 4.32RR87 pKa = 11.84ALKK90 pKa = 10.4FRR92 pKa = 11.84DD93 pKa = 3.46NFGKK97 pKa = 8.96GTYY100 pKa = 9.25DD101 pKa = 3.25QIGAKK106 pKa = 8.12VARR109 pKa = 11.84LSRR112 pKa = 11.84KK113 pKa = 8.17YY114 pKa = 10.47KK115 pKa = 9.98YY116 pKa = 9.09YY117 pKa = 10.73TEE119 pKa = 4.92GDD121 pKa = 3.67HH122 pKa = 6.55LTFDD126 pKa = 3.34AHH128 pKa = 5.35VTVEE132 pKa = 3.93MLRR135 pKa = 11.84LCHH138 pKa = 6.74RR139 pKa = 11.84FYY141 pKa = 11.35LRR143 pKa = 11.84CFNNDD148 pKa = 2.7SKK150 pKa = 11.5LLRR153 pKa = 11.84LCGKK157 pKa = 7.5TIRR160 pKa = 11.84NRR162 pKa = 11.84ALSRR166 pKa = 11.84NGEE169 pKa = 4.14RR170 pKa = 11.84YY171 pKa = 6.95TVNGTRR177 pKa = 11.84MSGDD181 pKa = 3.0VDD183 pKa = 3.58TSFGNSIINYY193 pKa = 7.63YY194 pKa = 9.96IIRR197 pKa = 11.84SVLADD202 pKa = 3.41LHH204 pKa = 6.78LSGDD208 pKa = 4.2AIVNGDD214 pKa = 4.03DD215 pKa = 3.89YY216 pKa = 11.38IIFTDD221 pKa = 3.9VPIDD225 pKa = 3.74GSAATTAFRR234 pKa = 11.84RR235 pKa = 11.84YY236 pKa = 11.08NMDD239 pKa = 3.29TKK241 pKa = 11.1LLPSSSNIHH250 pKa = 5.1TVSFCRR256 pKa = 11.84TKK258 pKa = 10.88LFYY261 pKa = 10.92HH262 pKa = 7.31PDD264 pKa = 3.06GHH266 pKa = 6.37PTMGFDD272 pKa = 3.79PVRR275 pKa = 11.84LKK277 pKa = 10.76KK278 pKa = 10.41IYY280 pKa = 9.66GCTNIMYY287 pKa = 9.42PFEE290 pKa = 7.25DD291 pKa = 3.5YY292 pKa = 9.85MQYY295 pKa = 11.28LEE297 pKa = 5.51LIHH300 pKa = 6.52HH301 pKa = 7.37ANYY304 pKa = 9.28MININSPIHH313 pKa = 5.89TNWKK317 pKa = 8.93PLPKK321 pKa = 10.3LEE323 pKa = 4.55SKK325 pKa = 10.12LKK327 pKa = 10.53KK328 pKa = 10.58YY329 pKa = 7.05MTRR332 pKa = 11.84SLEE335 pKa = 4.1RR336 pKa = 11.84VFNKK340 pKa = 9.91QLSNKK345 pKa = 9.31PYY347 pKa = 10.29DD348 pKa = 4.01YY349 pKa = 11.0DD350 pKa = 3.95YY351 pKa = 10.08LTPSYY356 pKa = 10.75IEE358 pKa = 4.87AYY360 pKa = 9.01PDD362 pKa = 3.25YY363 pKa = 11.33VYY365 pKa = 11.06DD366 pKa = 3.66RR367 pKa = 11.84KK368 pKa = 11.25VNILYY373 pKa = 7.59TKK375 pKa = 9.01PKK377 pKa = 9.73PPIEE381 pKa = 4.42FIINHH386 pKa = 6.3NIKK389 pKa = 10.4EE390 pKa = 4.05ISQCC394 pKa = 3.74

Molecular weight: 46.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

950

394

556

475.0

54.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.684 ± 0.987

1.684 ± 0.365

4.632 ± 1.042

4.316 ± 0.309

3.579 ± 0.602

3.158 ± 0.858

3.053 ± 0.004

7.158 ± 0.123

6.0 ± 0.981

10.0 ± 0.833

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.053 ± 0.004

4.421 ± 1.015

5.263 ± 0.041

3.684 ± 1.16

4.947 ± 1.467

8.211 ± 1.443

9.053 ± 1.492

4.737 ± 0.72

1.474 ± 0.587

5.895 ± 1.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski