Amino acid dipepetide frequency for Arthrobacter sp. Leaf69

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.958AlaAla: 21.958 ± 0.212	0.738AlaCys: 0.738 ± 0.027	7.447AlaAsp: 7.447 ± 0.087	8.372AlaGlu: 8.372 ± 0.091	3.714AlaPhe: 3.714 ± 0.063	13.958AlaGly: 13.958 ± 0.13	2.26AlaHis: 2.26 ± 0.049	4.978AlaIle: 4.978 ± 0.074	3.797AlaLys: 3.797 ± 0.075	13.119AlaLeu: 13.119 ± 0.126	2.781AlaMet: 2.781 ± 0.045	2.682AlaAsn: 2.682 ± 0.055	6.442AlaPro: 6.442 ± 0.108	3.741AlaGln: 3.741 ± 0.06	7.984AlaArg: 7.984 ± 0.11	7.058AlaSer: 7.058 ± 0.098	6.753AlaThr: 6.753 ± 0.082	11.733AlaVal: 11.733 ± 0.11	1.648AlaTrp: 1.648 ± 0.042	2.224AlaTyr: 2.224 ± 0.045	0.003AlaXaa: 0.003 ± 0.002
Cys	0.719CysAla: 0.719 ± 0.025	0.05CysCys: 0.05 ± 0.007	0.351CysAsp: 0.351 ± 0.02	0.305CysGlu: 0.305 ± 0.018	0.189CysPhe: 0.189 ± 0.011	0.681CysGly: 0.681 ± 0.029	0.135CysHis: 0.135 ± 0.01	0.257CysIle: 0.257 ± 0.015	0.104CysLys: 0.104 ± 0.01	0.558CysLeu: 0.558 ± 0.024	0.116CysMet: 0.116 ± 0.011	0.142CysAsn: 0.142 ± 0.011	0.352CysPro: 0.352 ± 0.021	0.158CysGln: 0.158 ± 0.011	0.398CysArg: 0.398 ± 0.017	0.376CysSer: 0.376 ± 0.019	0.404CysThr: 0.404 ± 0.019	0.426CysVal: 0.426 ± 0.02	0.091CysTrp: 0.091 ± 0.009	0.143CysTyr: 0.143 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.638AspAla: 7.638 ± 0.084	0.32AspCys: 0.32 ± 0.017	3.083AspAsp: 3.083 ± 0.062	3.382AspGlu: 3.382 ± 0.057	1.996AspPhe: 1.996 ± 0.045	5.781AspGly: 5.781 ± 0.071	1.2AspHis: 1.2 ± 0.032	2.392AspIle: 2.392 ± 0.052	1.464AspLys: 1.464 ± 0.04	5.48AspLeu: 5.48 ± 0.068	0.922AspMet: 0.922 ± 0.031	1.141AspAsn: 1.141 ± 0.03	3.757AspPro: 3.757 ± 0.057	1.6AspGln: 1.6 ± 0.046	3.536AspArg: 3.536 ± 0.054	2.83AspSer: 2.83 ± 0.042	2.837AspThr: 2.837 ± 0.06	5.128AspVal: 5.128 ± 0.072	0.877AspTrp: 0.877 ± 0.029	1.277AspTyr: 1.277 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	7.268GluAla: 7.268 ± 0.1	0.305GluCys: 0.305 ± 0.017	3.028GluAsp: 3.028 ± 0.058	3.334GluGlu: 3.334 ± 0.062	1.828GluPhe: 1.828 ± 0.033	3.931GluGly: 3.931 ± 0.061	1.414GluHis: 1.414 ± 0.035	2.62GluIle: 2.62 ± 0.057	1.659GluLys: 1.659 ± 0.036	7.08GluLeu: 7.08 ± 0.098	1.013GluMet: 1.013 ± 0.033	1.438GluAsn: 1.438 ± 0.038	2.852GluPro: 2.852 ± 0.05	2.252GluGln: 2.252 ± 0.048	4.04GluArg: 4.04 ± 0.065	3.008GluSer: 3.008 ± 0.051	2.951GluThr: 2.951 ± 0.046	4.17GluVal: 4.17 ± 0.071	0.811GluTrp: 0.811 ± 0.027	1.115GluTyr: 1.115 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	4.009PheAla: 4.009 ± 0.063	0.227PheCys: 0.227 ± 0.015	2.194PheAsp: 2.194 ± 0.042	1.723PheGlu: 1.723 ± 0.04	1.179PhePhe: 1.179 ± 0.034	3.475PheGly: 3.475 ± 0.059	0.659PheHis: 0.659 ± 0.025	1.354PheIle: 1.354 ± 0.033	0.826PheLys: 0.826 ± 0.029	3.061PheLeu: 3.061 ± 0.06	0.592PheMet: 0.592 ± 0.024	0.872PheAsn: 0.872 ± 0.031	1.525PhePro: 1.525 ± 0.035	0.901PheGln: 0.901 ± 0.028	1.931PheArg: 1.931 ± 0.044	1.995PheSer: 1.995 ± 0.045	2.104PheThr: 2.104 ± 0.049	2.555PheVal: 2.555 ± 0.051	0.462PheTrp: 0.462 ± 0.019	0.727PheTyr: 0.727 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	10.53GlyAla: 10.53 ± 0.117	0.608GlyCys: 0.608 ± 0.027	4.398GlyAsp: 4.398 ± 0.07	4.659GlyGlu: 4.659 ± 0.067	3.258GlyPhe: 3.258 ± 0.065	8.002GlyGly: 8.002 ± 0.1	2.029GlyHis: 2.029 ± 0.041	4.704GlyIle: 4.704 ± 0.067	3.107GlyLys: 3.107 ± 0.055	9.439GlyLeu: 9.439 ± 0.107	2.149GlyMet: 2.149 ± 0.047	2.252GlyAsn: 2.252 ± 0.049	4.315GlyPro: 4.315 ± 0.075	3.069GlyGln: 3.069 ± 0.063	6.134GlyArg: 6.134 ± 0.086	5.859GlySer: 5.859 ± 0.071	6.192GlyThr: 6.192 ± 0.087	7.352GlyVal: 7.352 ± 0.087	1.616GlyTrp: 1.616 ± 0.042	2.339GlyTyr: 2.339 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.274HisAla: 2.274 ± 0.042	0.155HisCys: 0.155 ± 0.011	1.2HisAsp: 1.2 ± 0.031	1.157HisGlu: 1.157 ± 0.031	0.706HisPhe: 0.706 ± 0.024	2.094HisGly: 2.094 ± 0.045	0.63HisHis: 0.63 ± 0.023	0.792HisIle: 0.792 ± 0.025	0.455HisLys: 0.455 ± 0.02	2.025HisLeu: 2.025 ± 0.041	0.356HisMet: 0.356 ± 0.018	0.465HisAsn: 0.465 ± 0.02	1.469HisPro: 1.469 ± 0.044	0.589HisGln: 0.589 ± 0.023	1.546HisArg: 1.546 ± 0.039	1.177HisSer: 1.177 ± 0.033	1.093HisThr: 1.093 ± 0.032	1.55HisVal: 1.55 ± 0.036	0.305HisTrp: 0.305 ± 0.02	0.459HisTyr: 0.459 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.794IleAla: 5.794 ± 0.079	0.327IleCys: 0.327 ± 0.017	2.654IleAsp: 2.654 ± 0.054	2.374IleGlu: 2.374 ± 0.048	1.414IlePhe: 1.414 ± 0.04	4.198IleGly: 4.198 ± 0.073	0.809IleHis: 0.809 ± 0.029	1.863IleIle: 1.863 ± 0.043	1.144IleLys: 1.144 ± 0.033	3.862IleLeu: 3.862 ± 0.069	0.788IleMet: 0.788 ± 0.026	1.249IleAsn: 1.249 ± 0.036	2.328IlePro: 2.328 ± 0.04	1.168IleGln: 1.168 ± 0.033	2.816IleArg: 2.816 ± 0.058	2.649IleSer: 2.649 ± 0.06	2.734IleThr: 2.734 ± 0.051	3.652IleVal: 3.652 ± 0.067	0.5IleTrp: 0.5 ± 0.021	0.851IleTyr: 0.851 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	3.672LysAla: 3.672 ± 0.07	0.112LysCys: 0.112 ± 0.01	1.793LysAsp: 1.793 ± 0.045	1.449LysGlu: 1.449 ± 0.042	0.811LysPhe: 0.811 ± 0.028	2.021LysGly: 2.021 ± 0.044	0.578LysHis: 0.578 ± 0.026	1.347LysIle: 1.347 ± 0.035	1.045LysLys: 1.045 ± 0.034	2.716LysLeu: 2.716 ± 0.057	0.601LysMet: 0.601 ± 0.023	0.799LysAsn: 0.799 ± 0.029	1.698LysPro: 1.698 ± 0.044	0.851LysGln: 0.851 ± 0.029	1.733LysArg: 1.733 ± 0.043	1.677LysSer: 1.677 ± 0.043	1.675LysThr: 1.675 ± 0.032	2.409LysVal: 2.409 ± 0.052	0.316LysTrp: 0.316 ± 0.016	0.638LysTyr: 0.638 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	14.747LeuAla: 14.747 ± 0.149	0.607LeuCys: 0.607 ± 0.023	6.297LeuAsp: 6.297 ± 0.081	5.442LeuGlu: 5.442 ± 0.066	2.996LeuPhe: 2.996 ± 0.065	9.246LeuGly: 9.246 ± 0.088	2.016LeuHis: 2.016 ± 0.043	4.312LeuIle: 4.312 ± 0.074	2.8LeuLys: 2.8 ± 0.057	10.637LeuLeu: 10.637 ± 0.136	2.027LeuMet: 2.027 ± 0.046	2.57LeuAsn: 2.57 ± 0.045	5.852LeuPro: 5.852 ± 0.079	2.956LeuGln: 2.956 ± 0.066	7.007LeuArg: 7.007 ± 0.08	6.046LeuSer: 6.046 ± 0.068	6.005LeuThr: 6.005 ± 0.077	8.331LeuVal: 8.331 ± 0.104	1.256LeuTrp: 1.256 ± 0.038	1.71LeuTyr: 1.71 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	2.618MetAla: 2.618 ± 0.05	0.127MetCys: 0.127 ± 0.009	1.049MetAsp: 1.049 ± 0.033	0.956MetGlu: 0.956 ± 0.03	0.613MetPhe: 0.613 ± 0.022	1.59MetGly: 1.59 ± 0.042	0.375MetHis: 0.375 ± 0.02	0.97MetIle: 0.97 ± 0.029	0.62MetLys: 0.62 ± 0.027	2.026MetLeu: 2.026 ± 0.041	0.396MetMet: 0.396 ± 0.017	0.576MetAsn: 0.576 ± 0.021	1.168MetPro: 1.168 ± 0.032	0.563MetGln: 0.563 ± 0.023	1.218MetArg: 1.218 ± 0.03	1.479MetSer: 1.479 ± 0.036	1.625MetThr: 1.625 ± 0.032	1.563MetVal: 1.563 ± 0.037	0.228MetTrp: 0.228 ± 0.013	0.325MetTyr: 0.325 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.901AsnAla: 2.901 ± 0.044	0.173AsnCys: 0.173 ± 0.012	1.297AsnAsp: 1.297 ± 0.034	1.139AsnGlu: 1.139 ± 0.035	0.812AsnPhe: 0.812 ± 0.03	2.31AsnGly: 2.31 ± 0.05	0.524AsnHis: 0.524 ± 0.023	1.066AsnIle: 1.066 ± 0.031	0.672AsnLys: 0.672 ± 0.029	2.251AsnLeu: 2.251 ± 0.047	0.46AsnMet: 0.46 ± 0.018	0.677AsnAsn: 0.677 ± 0.03	1.837AsnPro: 1.837 ± 0.042	0.771AsnGln: 0.771 ± 0.025	1.503AsnArg: 1.503 ± 0.034	1.357AsnSer: 1.357 ± 0.035	1.381AsnThr: 1.381 ± 0.033	1.936AsnVal: 1.936 ± 0.044	0.363AsnTrp: 0.363 ± 0.019	0.614AsnTyr: 0.614 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	8.441ProAla: 8.441 ± 0.125	0.218ProCys: 0.218 ± 0.015	3.803ProAsp: 3.803 ± 0.064	4.161ProGlu: 4.161 ± 0.067	1.699ProPhe: 1.699 ± 0.04	5.954ProGly: 5.954 ± 0.087	1.054ProHis: 1.054 ± 0.027	1.651ProIle: 1.651 ± 0.036	1.392ProLys: 1.392 ± 0.038	4.938ProLeu: 4.938 ± 0.066	1.003ProMet: 1.003 ± 0.028	1.106ProAsn: 1.106 ± 0.035	2.246ProPro: 2.246 ± 0.058	1.653ProGln: 1.653 ± 0.04	2.984ProArg: 2.984 ± 0.058	3.053ProSer: 3.053 ± 0.055	2.732ProThr: 2.732 ± 0.058	4.886ProVal: 4.886 ± 0.074	0.792ProTrp: 0.792 ± 0.027	1.049ProTyr: 1.049 ± 0.031	0.001ProXaa: 0.001 ± 0.001
Gln	3.878GlnAla: 3.878 ± 0.057	0.153GlnCys: 0.153 ± 0.012	1.703GlnAsp: 1.703 ± 0.034	1.587GlnGlu: 1.587 ± 0.043	0.879GlnPhe: 0.879 ± 0.031	2.36GlnGly: 2.36 ± 0.045	0.71GlnHis: 0.71 ± 0.022	1.381GlnIle: 1.381 ± 0.036	0.836GlnLys: 0.836 ± 0.024	3.896GlnLeu: 3.896 ± 0.078	0.594GlnMet: 0.594 ± 0.023	0.719GlnAsn: 0.719 ± 0.022	1.776GlnPro: 1.776 ± 0.037	1.398GlnGln: 1.398 ± 0.035	2.323GlnArg: 2.323 ± 0.055	1.66GlnSer: 1.66 ± 0.035	1.469GlnThr: 1.469 ± 0.037	2.304GlnVal: 2.304 ± 0.048	0.487GlnTrp: 0.487 ± 0.021	0.636GlnTyr: 0.636 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	7.16ArgAla: 7.16 ± 0.084	0.376ArgCys: 0.376 ± 0.018	3.542ArgAsp: 3.542 ± 0.064	3.761ArgGlu: 3.761 ± 0.063	2.226ArgPhe: 2.226 ± 0.046	4.908ArgGly: 4.908 ± 0.066	1.601ArgHis: 1.601 ± 0.04	3.374ArgIle: 3.374 ± 0.053	1.97ArgLys: 1.97 ± 0.045	7.002ArgLeu: 7.002 ± 0.087	1.53ArgMet: 1.53 ± 0.037	1.765ArgAsn: 1.765 ± 0.036	3.48ArgPro: 3.48 ± 0.058	2.357ArgGln: 2.357 ± 0.049	5.489ArgArg: 5.489 ± 0.085	3.879ArgSer: 3.879 ± 0.059	3.932ArgThr: 3.932 ± 0.056	4.677ArgVal: 4.677 ± 0.072	1.064ArgTrp: 1.064 ± 0.031	1.524ArgTyr: 1.524 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	7.534SerAla: 7.534 ± 0.103	0.363SerCys: 0.363 ± 0.017	2.833SerAsp: 2.833 ± 0.054	2.762SerGlu: 2.762 ± 0.053	2.06SerPhe: 2.06 ± 0.045	6.117SerGly: 6.117 ± 0.074	1.164SerHis: 1.164 ± 0.033	2.4SerIle: 2.4 ± 0.046	1.512SerLys: 1.512 ± 0.038	5.773SerLeu: 5.773 ± 0.063	1.332SerMet: 1.332 ± 0.028	1.374SerAsn: 1.374 ± 0.038	3.263SerPro: 3.263 ± 0.053	1.677SerGln: 1.677 ± 0.043	3.828SerArg: 3.828 ± 0.065	3.507SerSer: 3.507 ± 0.065	3.451SerThr: 3.451 ± 0.062	4.616SerVal: 4.616 ± 0.065	0.887SerTrp: 0.887 ± 0.032	1.444SerTyr: 1.444 ± 0.038	0.005SerXaa: 0.005 ± 0.003
Thr	8.16ThrAla: 8.16 ± 0.082	0.27ThrCys: 0.27 ± 0.017	3.112ThrAsp: 3.112 ± 0.059	3.093ThrGlu: 3.093 ± 0.051	1.826ThrPhe: 1.826 ± 0.038	6.0ThrGly: 6.0 ± 0.081	1.067ThrHis: 1.067 ± 0.032	2.44ThrIle: 2.44 ± 0.049	1.478ThrLys: 1.478 ± 0.04	5.584ThrLeu: 5.584 ± 0.08	1.024ThrMet: 1.024 ± 0.035	1.203ThrAsn: 1.203 ± 0.034	3.831ThrPro: 3.831 ± 0.071	1.444ThrGln: 1.444 ± 0.035	3.263ThrArg: 3.263 ± 0.052	3.343ThrSer: 3.343 ± 0.055	3.458ThrThr: 3.458 ± 0.066	5.588ThrVal: 5.588 ± 0.075	0.707ThrTrp: 0.707 ± 0.027	1.129ThrTyr: 1.129 ± 0.033	0.002ThrXaa: 0.002 ± 0.001
Val	10.265ValAla: 10.265 ± 0.099	0.553ValCys: 0.553 ± 0.024	4.93ValAsp: 4.93 ± 0.069	4.722ValGlu: 4.722 ± 0.071	2.776ValPhe: 2.776 ± 0.049	6.359ValGly: 6.359 ± 0.072	1.606ValHis: 1.606 ± 0.032	3.858ValIle: 3.858 ± 0.06	2.123ValLys: 2.123 ± 0.05	9.357ValLeu: 9.357 ± 0.107	1.723ValMet: 1.723 ± 0.044	2.063ValAsn: 2.063 ± 0.044	4.827ValPro: 4.827 ± 0.073	2.333ValGln: 2.333 ± 0.044	5.323ValArg: 5.323 ± 0.07	4.925ValSer: 4.925 ± 0.065	5.243ValThr: 5.243 ± 0.074	7.67ValVal: 7.67 ± 0.09	1.041ValTrp: 1.041 ± 0.03	1.495ValTyr: 1.495 ± 0.036	0.001ValXaa: 0.001 ± 0.001
Trp	1.49TrpAla: 1.49 ± 0.041	0.12TrpCys: 0.12 ± 0.011	0.758TrpAsp: 0.758 ± 0.027	0.619TrpGlu: 0.619 ± 0.025	0.567TrpPhe: 0.567 ± 0.024	0.991TrpGly: 0.991 ± 0.035	0.34TrpHis: 0.34 ± 0.016	0.672TrpIle: 0.672 ± 0.022	0.397TrpLys: 0.397 ± 0.018	1.803TrpLeu: 1.803 ± 0.047	0.328TrpMet: 0.328 ± 0.018	0.432TrpAsn: 0.432 ± 0.022	0.683TrpPro: 0.683 ± 0.022	0.578TrpGln: 0.578 ± 0.023	0.999TrpArg: 0.999 ± 0.03	0.833TrpSer: 0.833 ± 0.028	0.847TrpThr: 0.847 ± 0.028	1.033TrpVal: 1.033 ± 0.032	0.305TrpTrp: 0.305 ± 0.017	0.3TrpTyr: 0.3 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.334TyrAla: 2.334 ± 0.04	0.149TyrCys: 0.149 ± 0.011	1.169TyrAsp: 1.169 ± 0.033	1.119TyrGlu: 1.119 ± 0.034	0.821TyrPhe: 0.821 ± 0.027	2.037TyrGly: 2.037 ± 0.037	0.328TyrHis: 0.328 ± 0.017	0.742TyrIle: 0.742 ± 0.025	0.538TyrLys: 0.538 ± 0.022	2.254TyrLeu: 2.254 ± 0.043	0.348TyrMet: 0.348 ± 0.017	0.522TyrAsn: 0.522 ± 0.023	1.115TyrPro: 1.115 ± 0.031	0.692TyrGln: 0.692 ± 0.024	1.558TyrArg: 1.558 ± 0.038	1.189TyrSer: 1.189 ± 0.031	1.132TyrThr: 1.132 ± 0.036	1.613TyrVal: 1.613 ± 0.034	0.34TyrTrp: 0.34 ± 0.02	0.508TyrTyr: 0.508 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.003XaaThr: 0.003 ± 0.003	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3584 proteins (1172066 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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