Gemella asaccharolytica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillales incertae sedis; Bacillales Family XI. Incertae Sedis; Gemella

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1251 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133ZTP2|A0A133ZTP2_9BACL ABC transporter ATP-binding protein EcsA OS=Gemella asaccharolytica OX=502393 GN=HMPREF1871_00200 PE=4 SV=1
MM1 pKa = 6.85YY2 pKa = 7.8TTNLSNLEE10 pKa = 4.25SPHH13 pKa = 7.58LIYY16 pKa = 10.73SDD18 pKa = 3.81DD19 pKa = 4.27DD20 pKa = 4.27EE21 pKa = 5.0YY22 pKa = 11.87SQDD25 pKa = 4.46FNTEE29 pKa = 3.57LNYY32 pKa = 10.65NSEE35 pKa = 4.53IISDD39 pKa = 4.2YY40 pKa = 11.26ADD42 pKa = 2.93EE43 pKa = 5.25RR44 pKa = 11.84YY45 pKa = 10.43NCNEE49 pKa = 4.54LILDD53 pKa = 3.69RR54 pKa = 11.84TEE56 pKa = 5.95DD57 pKa = 3.75DD58 pKa = 3.88FSDD61 pKa = 3.97YY62 pKa = 11.33SMEE65 pKa = 4.17MGLTMNYY72 pKa = 10.37SYY74 pKa = 11.83DD75 pKa = 3.45NDD77 pKa = 3.45FKK79 pKa = 11.27YY80 pKa = 11.25DD81 pKa = 3.6EE82 pKa = 4.32VSISNDD88 pKa = 2.93QIMLEE93 pKa = 4.13NASDD97 pKa = 3.59KK98 pKa = 11.42

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133ZMH4|A0A133ZMH4_9BACL 50S ribosomal protein L3 OS=Gemella asaccharolytica OX=502393 GN=rplC PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.91VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.23NGRR28 pKa = 11.84NVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1251

0

1251

384050

37

3364

307.0

35.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.029 ± 0.079

0.847 ± 0.025

5.294 ± 0.069

7.659 ± 0.092

4.665 ± 0.077

5.54 ± 0.063

1.277 ± 0.025

10.343 ± 0.108

11.322 ± 0.118

9.166 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.035

6.839 ± 0.096

2.504 ± 0.038

2.298 ± 0.032

3.015 ± 0.053

6.243 ± 0.054

4.932 ± 0.066

5.908 ± 0.07

0.551 ± 0.021

4.38 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski