Amino acid dipepetide frequency for Helicobacter phage FrMEG235U

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.301AlaAla: 1.301 ± 0.509
0.867AlaCys: 0.867 ± 0.409
1.843AlaAsp: 1.843 ± 0.327
3.578AlaGlu: 3.578 ± 0.569
4.012AlaPhe: 4.012 ± 0.923
3.253AlaGly: 3.253 ± 0.925
0.651AlaHis: 0.651 ± 0.307
5.53AlaIle: 5.53 ± 1.102
8.566AlaLys: 8.566 ± 0.93
12.144AlaLeu: 12.144 ± 1.397
1.626AlaMet: 1.626 ± 0.426
7.264AlaAsn: 7.264 ± 1.018
1.084AlaPro: 1.084 ± 0.248
3.144AlaGln: 3.144 ± 0.506
2.819AlaArg: 2.819 ± 0.573
3.686AlaSer: 3.686 ± 0.576
2.711AlaThr: 2.711 ± 0.562
2.06AlaVal: 2.06 ± 0.5
0.217AlaTrp: 0.217 ± 0.162
2.385AlaTyr: 2.385 ± 0.604
0.0AlaXaa: 0.0 ± 0.0
Cys
0.434CysAla: 0.434 ± 0.234
0.108CysCys: 0.108 ± 0.109
0.759CysAsp: 0.759 ± 0.413
0.867CysGlu: 0.867 ± 0.336
0.867CysPhe: 0.867 ± 0.368
0.434CysGly: 0.434 ± 0.247
0.0CysHis: 0.0 ± 0.0
0.542CysIle: 0.542 ± 0.209
0.108CysLys: 0.108 ± 0.097
1.193CysLeu: 1.193 ± 0.425
0.108CysMet: 0.108 ± 0.1
0.217CysAsn: 0.217 ± 0.137
0.434CysPro: 0.434 ± 0.243
0.325CysGln: 0.325 ± 0.212
0.108CysArg: 0.108 ± 0.095
0.108CysSer: 0.108 ± 0.097
0.434CysThr: 0.434 ± 0.234
0.542CysVal: 0.542 ± 0.226
0.0CysTrp: 0.0 ± 0.0
0.108CysTyr: 0.108 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
2.06AspAla: 2.06 ± 0.522
0.325AspCys: 0.325 ± 0.205
1.41AspAsp: 1.41 ± 0.291
3.47AspGlu: 3.47 ± 0.585
3.361AspPhe: 3.361 ± 0.575
1.301AspGly: 1.301 ± 0.334
0.759AspHis: 0.759 ± 0.239
3.144AspIle: 3.144 ± 0.62
5.747AspLys: 5.747 ± 0.964
7.807AspLeu: 7.807 ± 0.943
1.084AspMet: 1.084 ± 0.485
4.229AspAsn: 4.229 ± 0.671
1.518AspPro: 1.518 ± 0.374
1.193AspGln: 1.193 ± 0.362
2.06AspArg: 2.06 ± 0.545
2.927AspSer: 2.927 ± 0.971
1.626AspThr: 1.626 ± 0.501
1.084AspVal: 1.084 ± 0.333
0.217AspTrp: 0.217 ± 0.162
3.47AspTyr: 3.47 ± 0.664
0.0AspXaa: 0.0 ± 0.0
Glu
6.614GluAla: 6.614 ± 0.9
0.434GluCys: 0.434 ± 0.209
2.385GluAsp: 2.385 ± 0.323
4.229GluGlu: 4.229 ± 0.72
3.795GluPhe: 3.795 ± 0.733
1.41GluGly: 1.41 ± 0.314
1.193GluHis: 1.193 ± 0.375
7.264GluIle: 7.264 ± 0.92
8.782GluLys: 8.782 ± 0.983
8.023GluLeu: 8.023 ± 0.775
2.06GluMet: 2.06 ± 0.559
6.289GluAsn: 6.289 ± 0.793
1.518GluPro: 1.518 ± 0.468
4.445GluGln: 4.445 ± 0.777
4.662GluArg: 4.662 ± 1.059
6.505GluSer: 6.505 ± 0.911
5.204GluThr: 5.204 ± 0.423
3.578GluVal: 3.578 ± 0.748
0.651GluTrp: 0.651 ± 0.205
2.602GluTyr: 2.602 ± 0.456
0.0GluXaa: 0.0 ± 0.0
Phe
1.518PheAla: 1.518 ± 0.487
0.542PheCys: 0.542 ± 0.227
2.494PheAsp: 2.494 ± 0.589
4.012PheGlu: 4.012 ± 0.64
3.686PhePhe: 3.686 ± 0.583
1.518PheGly: 1.518 ± 0.26
1.193PheHis: 1.193 ± 0.352
2.927PheIle: 2.927 ± 0.509
6.505PheLys: 6.505 ± 0.851
6.722PheLeu: 6.722 ± 1.212
0.434PheMet: 0.434 ± 0.271
3.361PheAsn: 3.361 ± 0.773
0.434PhePro: 0.434 ± 0.153
0.976PheGln: 0.976 ± 0.339
2.168PheArg: 2.168 ± 0.509
4.879PheSer: 4.879 ± 0.577
2.277PheThr: 2.277 ± 0.548
2.06PheVal: 2.06 ± 0.37
0.108PheTrp: 0.108 ± 0.101
2.06PheTyr: 2.06 ± 0.529
0.0PheXaa: 0.0 ± 0.0
Gly
3.036GlyAla: 3.036 ± 0.952
0.434GlyCys: 0.434 ± 0.223
1.626GlyAsp: 1.626 ± 0.469
2.277GlyGlu: 2.277 ± 0.345
3.144GlyPhe: 3.144 ± 0.582
3.144GlyGly: 3.144 ± 0.697
0.325GlyHis: 0.325 ± 0.253
2.06GlyIle: 2.06 ± 0.417
1.843GlyLys: 1.843 ± 0.344
6.072GlyLeu: 6.072 ± 0.641
1.193GlyMet: 1.193 ± 0.388
3.036GlyAsn: 3.036 ± 0.505
0.108GlyPro: 0.108 ± 0.119
0.976GlyGln: 0.976 ± 0.313
1.518GlyArg: 1.518 ± 0.368
3.47GlySer: 3.47 ± 0.497
1.193GlyThr: 1.193 ± 0.395
4.554GlyVal: 4.554 ± 1.25
0.0GlyTrp: 0.0 ± 0.0
2.06GlyTyr: 2.06 ± 0.415
0.0GlyXaa: 0.0 ± 0.0
His
1.084HisAla: 1.084 ± 0.29
0.325HisCys: 0.325 ± 0.21
0.759HisAsp: 0.759 ± 0.464
1.626HisGlu: 1.626 ± 0.458
0.867HisPhe: 0.867 ± 0.412
0.217HisGly: 0.217 ± 0.165
0.0HisHis: 0.0 ± 0.0
1.193HisIle: 1.193 ± 0.375
1.843HisLys: 1.843 ± 0.45
1.301HisLeu: 1.301 ± 0.31
0.217HisMet: 0.217 ± 0.127
1.084HisAsn: 1.084 ± 0.453
0.217HisPro: 0.217 ± 0.127
0.651HisGln: 0.651 ± 0.329
0.651HisArg: 0.651 ± 0.249
1.084HisSer: 1.084 ± 0.246
0.867HisThr: 0.867 ± 0.299
0.217HisVal: 0.217 ± 0.149
0.0HisTrp: 0.0 ± 0.0
0.867HisTyr: 0.867 ± 0.245
0.0HisXaa: 0.0 ± 0.0
Ile
5.53IleAla: 5.53 ± 0.952
0.434IleCys: 0.434 ± 0.25
4.445IleAsp: 4.445 ± 0.874
5.096IleGlu: 5.096 ± 0.753
1.952IlePhe: 1.952 ± 0.508
1.735IleGly: 1.735 ± 0.524
0.867IleHis: 0.867 ± 0.311
4.445IleIle: 4.445 ± 0.657
8.023IleLys: 8.023 ± 1.048
6.939IleLeu: 6.939 ± 0.564
0.867IleMet: 0.867 ± 0.254
4.445IleAsn: 4.445 ± 1.029
1.301IlePro: 1.301 ± 0.425
3.686IleGln: 3.686 ± 0.725
2.927IleArg: 2.927 ± 0.547
4.12IleSer: 4.12 ± 0.576
3.361IleThr: 3.361 ± 0.529
3.144IleVal: 3.144 ± 0.669
0.217IleTrp: 0.217 ± 0.134
2.602IleTyr: 2.602 ± 0.537
0.0IleXaa: 0.0 ± 0.0
Lys
9.433LysAla: 9.433 ± 1.194
0.325LysCys: 0.325 ± 0.216
6.939LysAsp: 6.939 ± 1.152
12.144LysGlu: 12.144 ± 1.568
3.361LysPhe: 3.361 ± 0.574
3.795LysGly: 3.795 ± 0.652
2.602LysHis: 2.602 ± 0.772
7.915LysIle: 7.915 ± 0.904
8.782LysLys: 8.782 ± 1.179
8.999LysLeu: 8.999 ± 1.065
1.084LysMet: 1.084 ± 0.439
8.674LysAsn: 8.674 ± 1.056
3.686LysPro: 3.686 ± 0.556
5.096LysGln: 5.096 ± 0.763
5.313LysArg: 5.313 ± 0.828
5.747LysSer: 5.747 ± 0.78
6.505LysThr: 6.505 ± 1.003
4.012LysVal: 4.012 ± 0.608
0.759LysTrp: 0.759 ± 0.215
2.602LysTyr: 2.602 ± 0.503
0.0LysXaa: 0.0 ± 0.0
Leu
7.264LeuAla: 7.264 ± 0.985
1.626LeuCys: 1.626 ± 0.633
4.012LeuAsp: 4.012 ± 0.517
12.577LeuGlu: 12.577 ± 1.443
3.903LeuPhe: 3.903 ± 0.893
6.072LeuGly: 6.072 ± 0.59
0.542LeuHis: 0.542 ± 0.236
5.313LeuIle: 5.313 ± 0.723
18.215LeuLys: 18.215 ± 1.528
6.397LeuLeu: 6.397 ± 0.819
2.385LeuMet: 2.385 ± 0.471
11.601LeuAsn: 11.601 ± 1.446
1.735LeuPro: 1.735 ± 0.445
4.445LeuGln: 4.445 ± 0.807
3.686LeuArg: 3.686 ± 0.483
5.421LeuSer: 5.421 ± 0.931
5.096LeuThr: 5.096 ± 0.597
3.795LeuVal: 3.795 ± 0.734
0.759LeuTrp: 0.759 ± 0.312
2.06LeuTyr: 2.06 ± 0.355
0.0LeuXaa: 0.0 ± 0.0
Met
0.651MetAla: 0.651 ± 0.217
0.108MetCys: 0.108 ± 0.085
1.41MetAsp: 1.41 ± 0.357
0.976MetGlu: 0.976 ± 0.286
1.518MetPhe: 1.518 ± 0.586
1.084MetGly: 1.084 ± 0.439
0.434MetHis: 0.434 ± 0.29
0.867MetIle: 0.867 ± 0.321
1.735MetLys: 1.735 ± 0.42
1.952MetLeu: 1.952 ± 0.356
0.108MetMet: 0.108 ± 0.105
1.952MetAsn: 1.952 ± 0.428
1.41MetPro: 1.41 ± 0.307
2.06MetGln: 2.06 ± 0.475
0.976MetArg: 0.976 ± 0.319
1.301MetSer: 1.301 ± 0.374
0.325MetThr: 0.325 ± 0.189
0.651MetVal: 0.651 ± 0.179
0.325MetTrp: 0.325 ± 0.206
0.325MetTyr: 0.325 ± 0.195
0.0MetXaa: 0.0 ± 0.0
Asn
8.891AsnAla: 8.891 ± 1.61
0.217AsnCys: 0.217 ± 0.132
3.361AsnAsp: 3.361 ± 0.547
7.373AsnGlu: 7.373 ± 0.999
3.578AsnPhe: 3.578 ± 0.585
2.385AsnGly: 2.385 ± 0.356
1.626AsnHis: 1.626 ± 0.418
3.47AsnIle: 3.47 ± 0.615
7.481AsnLys: 7.481 ± 0.776
7.373AsnLeu: 7.373 ± 0.924
1.735AsnMet: 1.735 ± 0.374
6.397AsnAsn: 6.397 ± 1.086
2.494AsnPro: 2.494 ± 0.62
5.747AsnGln: 5.747 ± 1.229
2.819AsnArg: 2.819 ± 0.518
4.12AsnSer: 4.12 ± 0.604
3.578AsnThr: 3.578 ± 0.59
2.277AsnVal: 2.277 ± 0.667
0.217AsnTrp: 0.217 ± 0.157
3.903AsnTyr: 3.903 ± 0.71
0.0AsnXaa: 0.0 ± 0.0
Pro
0.434ProAla: 0.434 ± 0.187
0.0ProCys: 0.0 ± 0.0
0.759ProAsp: 0.759 ± 0.239
1.084ProGlu: 1.084 ± 0.289
1.626ProPhe: 1.626 ± 0.403
0.325ProGly: 0.325 ± 0.145
0.0ProHis: 0.0 ± 0.0
2.385ProIle: 2.385 ± 0.442
3.253ProLys: 3.253 ± 0.594
2.711ProLeu: 2.711 ± 0.546
0.867ProMet: 0.867 ± 0.395
1.952ProAsn: 1.952 ± 0.381
0.217ProPro: 0.217 ± 0.147
0.976ProGln: 0.976 ± 0.291
0.651ProArg: 0.651 ± 0.196
3.036ProSer: 3.036 ± 0.384
2.06ProThr: 2.06 ± 0.477
0.325ProVal: 0.325 ± 0.148
0.108ProTrp: 0.108 ± 0.113
1.084ProTyr: 1.084 ± 0.43
0.0ProXaa: 0.0 ± 0.0
Gln
5.096GlnAla: 5.096 ± 0.952
0.325GlnCys: 0.325 ± 0.188
2.06GlnAsp: 2.06 ± 0.414
4.879GlnGlu: 4.879 ± 0.897
1.41GlnPhe: 1.41 ± 0.303
2.06GlnGly: 2.06 ± 0.443
0.651GlnHis: 0.651 ± 0.278
2.711GlnIle: 2.711 ± 0.445
5.747GlnLys: 5.747 ± 0.904
3.036GlnLeu: 3.036 ± 0.479
0.867GlnMet: 0.867 ± 0.352
4.445GlnAsn: 4.445 ± 0.904
0.759GlnPro: 0.759 ± 0.291
2.711GlnGln: 2.711 ± 0.677
1.41GlnArg: 1.41 ± 0.454
3.686GlnSer: 3.686 ± 0.643
2.385GlnThr: 2.385 ± 0.454
2.168GlnVal: 2.168 ± 0.336
0.542GlnTrp: 0.542 ± 0.212
0.867GlnTyr: 0.867 ± 0.278
0.0GlnXaa: 0.0 ± 0.0
Arg
3.361ArgAla: 3.361 ± 0.771
0.108ArgCys: 0.108 ± 0.095
2.168ArgAsp: 2.168 ± 0.401
3.361ArgGlu: 3.361 ± 0.659
2.277ArgPhe: 2.277 ± 0.496
1.301ArgGly: 1.301 ± 0.279
0.542ArgHis: 0.542 ± 0.192
3.036ArgIle: 3.036 ± 0.753
3.144ArgLys: 3.144 ± 0.616
5.204ArgLeu: 5.204 ± 0.795
0.759ArgMet: 0.759 ± 0.301
1.41ArgAsn: 1.41 ± 0.39
1.301ArgPro: 1.301 ± 0.366
1.518ArgGln: 1.518 ± 0.455
1.084ArgArg: 1.084 ± 0.345
3.036ArgSer: 3.036 ± 0.821
1.626ArgThr: 1.626 ± 0.389
1.952ArgVal: 1.952 ± 0.411
0.108ArgTrp: 0.108 ± 0.084
1.952ArgTyr: 1.952 ± 0.513
0.0ArgXaa: 0.0 ± 0.0
Ser
4.445SerAla: 4.445 ± 0.676
0.434SerCys: 0.434 ± 0.3
4.988SerAsp: 4.988 ± 0.488
5.638SerGlu: 5.638 ± 0.871
3.686SerPhe: 3.686 ± 0.569
4.229SerGly: 4.229 ± 0.95
1.084SerHis: 1.084 ± 0.311
3.144SerIle: 3.144 ± 0.475
5.747SerLys: 5.747 ± 0.681
8.023SerLeu: 8.023 ± 1.136
1.735SerMet: 1.735 ± 0.442
3.903SerAsn: 3.903 ± 0.595
1.301SerPro: 1.301 ± 0.265
2.819SerGln: 2.819 ± 0.466
1.843SerArg: 1.843 ± 0.434
2.927SerSer: 2.927 ± 0.464
2.06SerThr: 2.06 ± 0.496
4.988SerVal: 4.988 ± 1.19
0.325SerTrp: 0.325 ± 0.171
2.819SerTyr: 2.819 ± 0.356
0.0SerXaa: 0.0 ± 0.0
Thr
2.385ThrAla: 2.385 ± 0.666
0.325ThrCys: 0.325 ± 0.178
2.494ThrAsp: 2.494 ± 0.501
2.927ThrGlu: 2.927 ± 0.556
1.626ThrPhe: 1.626 ± 0.582
2.711ThrGly: 2.711 ± 0.664
1.084ThrHis: 1.084 ± 0.235
3.036ThrIle: 3.036 ± 0.541
4.12ThrLys: 4.12 ± 0.805
5.096ThrLeu: 5.096 ± 0.747
0.759ThrMet: 0.759 ± 0.221
3.578ThrAsn: 3.578 ± 0.475
2.494ThrPro: 2.494 ± 0.544
3.361ThrGln: 3.361 ± 0.57
1.518ThrArg: 1.518 ± 0.539
2.819ThrSer: 2.819 ± 0.451
2.711ThrThr: 2.711 ± 0.495
1.084ThrVal: 1.084 ± 0.447
0.434ThrTrp: 0.434 ± 0.205
1.518ThrTyr: 1.518 ± 0.467
0.0ThrXaa: 0.0 ± 0.0
Val
3.036ValAla: 3.036 ± 0.561
0.325ValCys: 0.325 ± 0.172
2.927ValAsp: 2.927 ± 0.967
2.277ValGlu: 2.277 ± 0.447
3.144ValPhe: 3.144 ± 0.633
3.686ValGly: 3.686 ± 0.649
0.217ValHis: 0.217 ± 0.166
3.47ValIle: 3.47 ± 0.622
4.12ValLys: 4.12 ± 0.613
4.12ValLeu: 4.12 ± 0.692
1.301ValMet: 1.301 ± 0.46
2.168ValAsn: 2.168 ± 0.756
0.651ValPro: 0.651 ± 0.273
0.867ValGln: 0.867 ± 0.255
1.41ValArg: 1.41 ± 0.283
4.337ValSer: 4.337 ± 1.311
0.651ValThr: 0.651 ± 0.167
2.168ValVal: 2.168 ± 0.445
0.325ValTrp: 0.325 ± 0.186
0.759ValTyr: 0.759 ± 0.343
0.0ValXaa: 0.0 ± 0.0
Trp
0.108TrpAla: 0.108 ± 0.116
0.108TrpCys: 0.108 ± 0.113
0.108TrpAsp: 0.108 ± 0.084
0.542TrpGlu: 0.542 ± 0.259
0.0TrpPhe: 0.0 ± 0.0
0.542TrpGly: 0.542 ± 0.264
0.108TrpHis: 0.108 ± 0.095
0.325TrpIle: 0.325 ± 0.2
0.325TrpLys: 0.325 ± 0.18
0.217TrpLeu: 0.217 ± 0.168
0.217TrpMet: 0.217 ± 0.145
0.434TrpAsn: 0.434 ± 0.245
0.0TrpPro: 0.0 ± 0.0
0.325TrpGln: 0.325 ± 0.168
0.217TrpArg: 0.217 ± 0.157
0.434TrpSer: 0.434 ± 0.268
0.325TrpThr: 0.325 ± 0.173
0.759TrpVal: 0.759 ± 0.313
0.0TrpTrp: 0.0 ± 0.0
0.217TrpTyr: 0.217 ± 0.21
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.06TyrAla: 2.06 ± 0.515
0.325TyrCys: 0.325 ± 0.228
1.843TyrAsp: 1.843 ± 0.297
2.711TyrGlu: 2.711 ± 0.428
2.06TyrPhe: 2.06 ± 0.629
0.867TyrGly: 0.867 ± 0.233
1.193TyrHis: 1.193 ± 0.314
3.361TyrIle: 3.361 ± 0.757
3.686TyrLys: 3.686 ± 0.526
3.578TyrLeu: 3.578 ± 0.63
0.651TyrMet: 0.651 ± 0.186
2.602TyrAsn: 2.602 ± 0.414
1.301TyrPro: 1.301 ± 0.301
2.494TyrGln: 2.494 ± 0.487
1.41TyrArg: 1.41 ± 0.401
2.494TyrSer: 2.494 ± 0.443
1.084TyrThr: 1.084 ± 0.416
0.542TyrVal: 0.542 ± 0.301
0.0TyrTrp: 0.0 ± 0.0
1.41TyrTyr: 1.41 ± 0.441
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 35 proteins (9224 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski