Amino acid dipepetide frequency for Lepisosteus oculatus (Spotted gar)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.145AlaAla: 6.145 ± 0.035	1.368AlaCys: 1.368 ± 0.013	3.118AlaAsp: 3.118 ± 0.017	4.868AlaGlu: 4.868 ± 0.026	2.473AlaPhe: 2.473 ± 0.015	4.636AlaGly: 4.636 ± 0.028	1.494AlaHis: 1.494 ± 0.011	2.784AlaIle: 2.784 ± 0.017	3.448AlaLys: 3.448 ± 0.022	6.751AlaLeu: 6.751 ± 0.034	1.5AlaMet: 1.5 ± 0.012	2.072AlaAsn: 2.072 ± 0.013	3.584AlaPro: 3.584 ± 0.029	3.048AlaGln: 3.048 ± 0.021	3.394AlaArg: 3.394 ± 0.019	5.392AlaSer: 5.392 ± 0.026	3.269AlaThr: 3.269 ± 0.018	5.097AlaVal: 5.097 ± 0.023	0.693AlaTrp: 0.693 ± 0.009	1.508AlaTyr: 1.508 ± 0.012	0.002AlaXaa: 0.002 ± 0.0
Cys	1.34CysAla: 1.34 ± 0.013	0.676CysCys: 0.676 ± 0.01	1.124CysAsp: 1.124 ± 0.018	1.341CysGlu: 1.341 ± 0.018	0.935CysPhe: 0.935 ± 0.01	1.604CysGly: 1.604 ± 0.02	0.627CysHis: 0.627 ± 0.009	1.045CysIle: 1.045 ± 0.014	1.215CysLys: 1.215 ± 0.015	2.224CysLeu: 2.224 ± 0.018	0.441CysMet: 0.441 ± 0.006	0.879CysAsn: 0.879 ± 0.011	1.366CysPro: 1.366 ± 0.02	1.11CysGln: 1.11 ± 0.016	1.353CysArg: 1.353 ± 0.014	2.149CysSer: 2.149 ± 0.021	1.28CysThr: 1.28 ± 0.014	1.517CysVal: 1.517 ± 0.017	0.306CysTrp: 0.306 ± 0.006	0.643CysTyr: 0.643 ± 0.008	0.001CysXaa: 0.001 ± 0.0
Asp	2.798AspAla: 2.798 ± 0.015	1.179AspCys: 1.179 ± 0.014	2.682AspAsp: 2.682 ± 0.023	3.548AspGlu: 3.548 ± 0.022	2.143AspPhe: 2.143 ± 0.012	3.365AspGly: 3.365 ± 0.026	1.129AspHis: 1.129 ± 0.009	2.739AspIle: 2.739 ± 0.018	2.69AspLys: 2.69 ± 0.019	4.937AspLeu: 4.937 ± 0.022	1.16AspMet: 1.16 ± 0.01	1.858AspAsn: 1.858 ± 0.017	2.852AspPro: 2.852 ± 0.017	1.839AspGln: 1.839 ± 0.014	2.725AspArg: 2.725 ± 0.019	4.467AspSer: 4.467 ± 0.025	2.616AspThr: 2.616 ± 0.016	3.177AspVal: 3.177 ± 0.019	0.671AspTrp: 0.671 ± 0.007	1.57AspTyr: 1.57 ± 0.011	0.001AspXaa: 0.001 ± 0.0
Glu	4.777GluAla: 4.777 ± 0.027	1.32GluCys: 1.32 ± 0.02	4.427GluAsp: 4.427 ± 0.022	7.925GluGlu: 7.925 ± 0.056	2.077GluPhe: 2.077 ± 0.013	4.363GluGly: 4.363 ± 0.025	1.496GluHis: 1.496 ± 0.013	3.195GluIle: 3.195 ± 0.022	5.148GluLys: 5.148 ± 0.033	6.346GluLeu: 6.346 ± 0.037	1.718GluMet: 1.718 ± 0.014	2.998GluAsn: 2.998 ± 0.017	2.905GluPro: 2.905 ± 0.021	3.214GluGln: 3.214 ± 0.02	4.278GluArg: 4.278 ± 0.028	4.592GluSer: 4.592 ± 0.025	3.522GluThr: 3.522 ± 0.021	4.311GluVal: 4.311 ± 0.023	0.723GluTrp: 0.723 ± 0.007	1.69GluTyr: 1.69 ± 0.015	0.001GluXaa: 0.001 ± 0.0
Phe	2.025PheAla: 2.025 ± 0.014	0.995PheCys: 0.995 ± 0.009	1.76PheAsp: 1.76 ± 0.013	2.024PheGlu: 2.024 ± 0.014	1.638PhePhe: 1.638 ± 0.015	2.222PheGly: 2.222 ± 0.017	1.022PheHis: 1.022 ± 0.009	1.945PheIle: 1.945 ± 0.015	1.888PheLys: 1.888 ± 0.014	4.035PheLeu: 4.035 ± 0.021	0.772PheMet: 0.772 ± 0.008	1.465PheAsn: 1.465 ± 0.011	1.899PhePro: 1.899 ± 0.017	1.745PheGln: 1.745 ± 0.013	1.969PheArg: 1.969 ± 0.012	3.428PheSer: 3.428 ± 0.018	2.227PheThr: 2.227 ± 0.016	2.291PheVal: 2.291 ± 0.015	0.473PheTrp: 0.473 ± 0.006	1.241PheTyr: 1.241 ± 0.012	0.001PheXaa: 0.001 ± 0.0
Gly	4.152GlyAla: 4.152 ± 0.028	1.313GlyCys: 1.313 ± 0.016	3.18GlyAsp: 3.18 ± 0.023	4.254GlyGlu: 4.254 ± 0.031	2.451GlyPhe: 2.451 ± 0.02	4.831GlyGly: 4.831 ± 0.041	1.591GlyHis: 1.591 ± 0.013	2.769GlyIle: 2.769 ± 0.021	3.799GlyLys: 3.799 ± 0.022	5.488GlyLeu: 5.488 ± 0.029	1.367GlyMet: 1.367 ± 0.011	2.497GlyAsn: 2.497 ± 0.016	3.297GlyPro: 3.297 ± 0.051	2.83GlyGln: 2.83 ± 0.021	3.664GlyArg: 3.664 ± 0.025	5.773GlySer: 5.773 ± 0.031	3.513GlyThr: 3.513 ± 0.022	3.833GlyVal: 3.833 ± 0.021	0.77GlyTrp: 0.77 ± 0.01	1.899GlyTyr: 1.899 ± 0.018	0.002GlyXaa: 0.002 ± 0.0
His	1.323HisAla: 1.323 ± 0.01	0.773HisCys: 0.773 ± 0.01	0.917HisAsp: 0.917 ± 0.009	1.245HisGlu: 1.245 ± 0.011	1.061HisPhe: 1.061 ± 0.01	1.472HisGly: 1.472 ± 0.013	0.865HisHis: 0.865 ± 0.012	1.253HisIle: 1.253 ± 0.01	1.256HisLys: 1.256 ± 0.012	2.638HisLeu: 2.638 ± 0.018	0.571HisMet: 0.571 ± 0.008	0.944HisAsn: 0.944 ± 0.009	1.507HisPro: 1.507 ± 0.015	1.167HisGln: 1.167 ± 0.012	1.575HisArg: 1.575 ± 0.014	2.373HisSer: 2.373 ± 0.014	1.49HisThr: 1.49 ± 0.016	1.426HisVal: 1.426 ± 0.011	0.343HisTrp: 0.343 ± 0.005	0.85HisTyr: 0.85 ± 0.008	0.001HisXaa: 0.001 ± 0.0
Ile	2.756IleAla: 2.756 ± 0.017	1.124IleCys: 1.124 ± 0.01	2.16IleAsp: 2.16 ± 0.015	2.634IleGlu: 2.634 ± 0.021	1.88IlePhe: 1.88 ± 0.017	2.358IleGly: 2.358 ± 0.017	1.237IleHis: 1.237 ± 0.012	2.438IleIle: 2.438 ± 0.02	2.705IleLys: 2.705 ± 0.019	4.51IleLeu: 4.51 ± 0.023	1.019IleMet: 1.019 ± 0.01	1.952IleAsn: 1.952 ± 0.016	2.585IlePro: 2.585 ± 0.015	2.242IleGln: 2.242 ± 0.017	2.44IleArg: 2.44 ± 0.015	3.832IleSer: 3.832 ± 0.02	2.779IleThr: 2.779 ± 0.02	2.755IleVal: 2.755 ± 0.023	0.51IleTrp: 0.51 ± 0.007	1.432IleTyr: 1.432 ± 0.012	0.001IleXaa: 0.001 ± 0.0
Lys	3.887LysAla: 3.887 ± 0.024	1.126LysCys: 1.126 ± 0.013	3.323LysAsp: 3.323 ± 0.023	5.102LysGlu: 5.102 ± 0.034	1.662LysPhe: 1.662 ± 0.013	3.348LysGly: 3.348 ± 0.031	1.438LysHis: 1.438 ± 0.012	2.779LysIle: 2.779 ± 0.018	4.792LysLys: 4.792 ± 0.033	5.13LysLeu: 5.13 ± 0.024	1.493LysMet: 1.493 ± 0.011	2.415LysAsn: 2.415 ± 0.016	2.987LysPro: 2.987 ± 0.02	2.717LysGln: 2.717 ± 0.018	3.49LysArg: 3.49 ± 0.022	4.029LysSer: 4.029 ± 0.025	3.34LysThr: 3.34 ± 0.02	3.551LysVal: 3.551 ± 0.022	0.628LysTrp: 0.628 ± 0.008	1.619LysTyr: 1.619 ± 0.012	0.002LysXaa: 0.002 ± 0.0
Leu	6.215LeuAla: 6.215 ± 0.027	2.312LeuCys: 2.312 ± 0.017	4.719LeuAsp: 4.719 ± 0.023	6.798LeuGlu: 6.798 ± 0.04	3.479LeuPhe: 3.479 ± 0.022	5.344LeuGly: 5.344 ± 0.029	2.668LeuHis: 2.668 ± 0.019	3.951LeuIle: 3.951 ± 0.023	6.021LeuLys: 6.021 ± 0.029	9.961LeuLeu: 9.961 ± 0.049	1.957LeuMet: 1.957 ± 0.014	3.661LeuAsn: 3.661 ± 0.017	5.375LeuPro: 5.375 ± 0.028	5.665LeuGln: 5.665 ± 0.03	5.643LeuArg: 5.643 ± 0.028	8.204LeuSer: 8.204 ± 0.033	5.028LeuThr: 5.028 ± 0.021	5.386LeuVal: 5.386 ± 0.027	1.087LeuTrp: 1.087 ± 0.01	2.69LeuTyr: 2.69 ± 0.015	0.003LeuXaa: 0.003 ± 0.0
Met	1.771MetAla: 1.771 ± 0.013	0.443MetCys: 0.443 ± 0.006	1.294MetAsp: 1.294 ± 0.012	1.859MetGlu: 1.859 ± 0.014	0.81MetPhe: 0.81 ± 0.008	1.306MetGly: 1.306 ± 0.012	0.47MetHis: 0.47 ± 0.006	0.902MetIle: 0.902 ± 0.01	1.514MetLys: 1.514 ± 0.011	1.915MetLeu: 1.915 ± 0.016	0.602MetMet: 0.602 ± 0.008	0.902MetAsn: 0.902 ± 0.01	1.016MetPro: 1.016 ± 0.011	0.951MetGln: 0.951 ± 0.01	1.083MetArg: 1.083 ± 0.01	1.701MetSer: 1.701 ± 0.013	1.176MetThr: 1.176 ± 0.009	1.376MetVal: 1.376 ± 0.011	0.239MetTrp: 0.239 ± 0.004	0.635MetTyr: 0.635 ± 0.007	0.0MetXaa: 0.0 ± 0.0
Asn	2.148AsnAla: 2.148 ± 0.016	0.923AsnCys: 0.923 ± 0.011	1.642AsnAsp: 1.642 ± 0.014	2.274AsnGlu: 2.274 ± 0.015	1.438AsnPhe: 1.438 ± 0.011	2.731AsnGly: 2.731 ± 0.023	0.901AsnHis: 0.901 ± 0.009	2.227AsnIle: 2.227 ± 0.014	2.28AsnLys: 2.28 ± 0.016	3.654AsnLeu: 3.654 ± 0.018	0.972AsnMet: 0.972 ± 0.01	1.707AsnAsn: 1.707 ± 0.016	2.214AsnPro: 2.214 ± 0.015	1.588AsnGln: 1.588 ± 0.014	1.991AsnArg: 1.991 ± 0.012	3.252AsnSer: 3.252 ± 0.018	2.185AsnThr: 2.185 ± 0.016	2.35AsnVal: 2.35 ± 0.018	0.465AsnTrp: 0.465 ± 0.007	1.166AsnTyr: 1.166 ± 0.013	0.001AsnXaa: 0.001 ± 0.0
Pro	4.41ProAla: 4.41 ± 0.033	1.156ProCys: 1.156 ± 0.016	2.757ProAsp: 2.757 ± 0.016	4.0ProGlu: 4.0 ± 0.022	1.849ProPhe: 1.849 ± 0.015	4.487ProGly: 4.487 ± 0.069	1.381ProHis: 1.381 ± 0.014	1.835ProIle: 1.835 ± 0.015	2.62ProLys: 2.62 ± 0.021	4.834ProLeu: 4.834 ± 0.021	0.937ProMet: 0.937 ± 0.01	1.788ProAsn: 1.788 ± 0.013	5.215ProPro: 5.215 ± 0.056	2.606ProGln: 2.606 ± 0.019	2.877ProArg: 2.877 ± 0.022	5.351ProSer: 5.351 ± 0.036	2.748ProThr: 2.748 ± 0.02	3.845ProVal: 3.845 ± 0.021	0.572ProTrp: 0.572 ± 0.008	1.379ProTyr: 1.379 ± 0.013	0.003ProXaa: 0.003 ± 0.001
Gln	3.369GlnAla: 3.369 ± 0.019	1.08GlnCys: 1.08 ± 0.015	2.326GlnAsp: 2.326 ± 0.014	3.709GlnGlu: 3.709 ± 0.025	1.426GlnPhe: 1.426 ± 0.012	2.831GlnGly: 2.831 ± 0.02	1.304GlnHis: 1.304 ± 0.011	2.034GlnIle: 2.034 ± 0.014	2.858GlnLys: 2.858 ± 0.017	4.53GlnLeu: 4.53 ± 0.029	1.077GlnMet: 1.077 ± 0.01	1.881GlnAsn: 1.881 ± 0.014	2.527GlnPro: 2.527 ± 0.02	3.156GlnGln: 3.156 ± 0.039	2.917GlnArg: 2.917 ± 0.019	3.355GlnSer: 3.355 ± 0.02	2.391GlnThr: 2.391 ± 0.016	2.761GlnVal: 2.761 ± 0.015	0.565GlnTrp: 0.565 ± 0.007	1.305GlnTyr: 1.305 ± 0.011	0.002GlnXaa: 0.002 ± 0.0
Arg	3.677ArgAla: 3.677 ± 0.022	1.266ArgCys: 1.266 ± 0.016	2.854ArgAsp: 2.854 ± 0.016	4.059ArgGlu: 4.059 ± 0.029	1.966ArgPhe: 1.966 ± 0.013	3.454ArgGly: 3.454 ± 0.029	1.522ArgHis: 1.522 ± 0.011	2.467ArgIle: 2.467 ± 0.015	3.726ArgLys: 3.726 ± 0.023	5.312ArgLeu: 5.312 ± 0.026	1.229ArgMet: 1.229 ± 0.011	2.182ArgAsn: 2.182 ± 0.014	2.917ArgPro: 2.917 ± 0.021	2.717ArgGln: 2.717 ± 0.02	4.317ArgArg: 4.317 ± 0.03	4.401ArgSer: 4.401 ± 0.026	2.869ArgThr: 2.869 ± 0.016	3.285ArgVal: 3.285 ± 0.02	0.681ArgTrp: 0.681 ± 0.009	1.592ArgTyr: 1.592 ± 0.012	0.002ArgXaa: 0.002 ± 0.0
Ser	5.625SerAla: 5.625 ± 0.025	2.024SerCys: 2.024 ± 0.02	4.123SerAsp: 4.123 ± 0.025	5.242SerGlu: 5.242 ± 0.029	3.168SerPhe: 3.168 ± 0.018	5.509SerGly: 5.509 ± 0.023	2.056SerHis: 2.056 ± 0.014	3.317SerIle: 3.317 ± 0.019	4.147SerLys: 4.147 ± 0.024	8.306SerLeu: 8.306 ± 0.032	1.648SerMet: 1.648 ± 0.012	2.847SerAsn: 2.847 ± 0.016	5.737SerPro: 5.737 ± 0.039	3.863SerGln: 3.863 ± 0.022	4.617SerArg: 4.617 ± 0.029	9.59SerSer: 9.59 ± 0.054	4.66SerThr: 4.66 ± 0.029	5.378SerVal: 5.378 ± 0.025	1.031SerTrp: 1.031 ± 0.011	2.203SerTyr: 2.203 ± 0.016	0.002SerXaa: 0.002 ± 0.0
Thr	4.082ThrAla: 4.082 ± 0.021	1.344ThrCys: 1.344 ± 0.017	2.741ThrAsp: 2.741 ± 0.017	3.75ThrGlu: 3.75 ± 0.02	2.124ThrPhe: 2.124 ± 0.016	3.714ThrGly: 3.714 ± 0.021	1.244ThrHis: 1.244 ± 0.014	2.428ThrIle: 2.428 ± 0.018	2.66ThrLys: 2.66 ± 0.016	5.297ThrLeu: 5.297 ± 0.022	1.127ThrMet: 1.127 ± 0.009	1.798ThrAsn: 1.798 ± 0.014	3.441ThrPro: 3.441 ± 0.022	2.267ThrGln: 2.267 ± 0.016	2.456ThrArg: 2.456 ± 0.016	4.537ThrSer: 4.537 ± 0.028	3.032ThrThr: 3.032 ± 0.034	4.237ThrVal: 4.237 ± 0.026	0.653ThrTrp: 0.653 ± 0.009	1.449ThrTyr: 1.449 ± 0.011	0.002ThrXaa: 0.002 ± 0.0
Val	4.007ValAla: 4.007 ± 0.02	1.707ValCys: 1.707 ± 0.017	2.983ValAsp: 2.983 ± 0.017	3.958ValGlu: 3.958 ± 0.023	2.738ValPhe: 2.738 ± 0.018	3.342ValGly: 3.342 ± 0.023	1.546ValHis: 1.546 ± 0.012	3.091ValIle: 3.091 ± 0.022	3.712ValLys: 3.712 ± 0.023	6.455ValLeu: 6.455 ± 0.031	1.408ValMet: 1.408 ± 0.011	2.468ValAsn: 2.468 ± 0.018	3.446ValPro: 3.446 ± 0.02	2.909ValGln: 2.909 ± 0.016	3.246ValArg: 3.246 ± 0.018	5.337ValSer: 5.337 ± 0.025	3.907ValThr: 3.907 ± 0.026	4.165ValVal: 4.165 ± 0.024	0.777ValTrp: 0.777 ± 0.009	1.89ValTyr: 1.89 ± 0.014	0.002ValXaa: 0.002 ± 0.0
Trp	0.707TrpAla: 0.707 ± 0.008	0.25TrpCys: 0.25 ± 0.005	0.645TrpAsp: 0.645 ± 0.008	0.782TrpGlu: 0.782 ± 0.009	0.463TrpPhe: 0.463 ± 0.007	0.676TrpGly: 0.676 ± 0.012	0.292TrpHis: 0.292 ± 0.004	0.586TrpIle: 0.586 ± 0.007	0.809TrpLys: 0.809 ± 0.009	1.156TrpLeu: 1.156 ± 0.011	0.324TrpMet: 0.324 ± 0.005	0.554TrpAsn: 0.554 ± 0.007	0.438TrpPro: 0.438 ± 0.006	0.507TrpGln: 0.507 ± 0.007	0.715TrpArg: 0.715 ± 0.008	0.916TrpSer: 0.916 ± 0.012	0.685TrpThr: 0.685 ± 0.009	0.729TrpVal: 0.729 ± 0.008	0.182TrpTrp: 0.182 ± 0.004	0.36TrpTyr: 0.36 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.424TyrAla: 1.424 ± 0.011	0.797TyrCys: 0.797 ± 0.01	1.37TyrAsp: 1.37 ± 0.012	1.654TyrGlu: 1.654 ± 0.013	1.284TyrPhe: 1.284 ± 0.012	1.681TyrGly: 1.681 ± 0.014	0.763TyrHis: 0.763 ± 0.007	1.561TyrIle: 1.561 ± 0.012	1.57TyrLys: 1.57 ± 0.014	2.687TyrLeu: 2.687 ± 0.018	0.641TyrMet: 0.641 ± 0.007	1.243TyrAsn: 1.243 ± 0.011	1.336TyrPro: 1.336 ± 0.012	1.261TyrGln: 1.261 ± 0.012	1.739TyrArg: 1.739 ± 0.014	2.413TyrSer: 2.413 ± 0.015	1.664TyrThr: 1.664 ± 0.014	1.624TyrVal: 1.624 ± 0.012	0.408TyrTrp: 0.408 ± 0.008	1.05TyrTyr: 1.05 ± 0.01	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.002XaaGly: 0.002 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.003XaaLeu: 0.003 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.004XaaPro: 0.004 ± 0.001	0.001XaaGln: 0.001 ± 0.0	0.002XaaArg: 0.002 ± 0.0	0.003XaaSer: 0.003 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.088XaaXaa: 0.088 ± 0.015

Statistics based on 22463 proteins (13406100 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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