Amino acid dipepetide frequency for Clostridium acidisoli DSM 12555

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.255AlaAla: 4.255 ± 0.082	0.655AlaCys: 0.655 ± 0.025	2.736AlaAsp: 2.736 ± 0.047	3.423AlaGlu: 3.423 ± 0.062	2.56AlaPhe: 2.56 ± 0.051	3.896AlaGly: 3.896 ± 0.07	0.845AlaHis: 0.845 ± 0.03	5.85AlaIle: 5.85 ± 0.08	4.596AlaLys: 4.596 ± 0.071	5.789AlaLeu: 5.789 ± 0.091	1.564AlaMet: 1.564 ± 0.041	2.769AlaAsn: 2.769 ± 0.058	1.386AlaPro: 1.386 ± 0.035	1.385AlaGln: 1.385 ± 0.04	1.819AlaArg: 1.819 ± 0.041	3.704AlaSer: 3.704 ± 0.071	2.913AlaThr: 2.913 ± 0.07	4.172AlaVal: 4.172 ± 0.06	0.405AlaTrp: 0.405 ± 0.018	2.189AlaTyr: 2.189 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	0.609CysAla: 0.609 ± 0.021	0.188CysCys: 0.188 ± 0.012	0.646CysAsp: 0.646 ± 0.024	0.743CysGlu: 0.743 ± 0.024	0.565CysPhe: 0.565 ± 0.025	1.024CysGly: 1.024 ± 0.03	0.193CysHis: 0.193 ± 0.012	1.21CysIle: 1.21 ± 0.035	1.004CysLys: 1.004 ± 0.033	0.89CysLeu: 0.89 ± 0.029	0.299CysMet: 0.299 ± 0.017	0.708CysAsn: 0.708 ± 0.026	0.443CysPro: 0.443 ± 0.021	0.196CysGln: 0.196 ± 0.014	0.341CysArg: 0.341 ± 0.018	0.821CysSer: 0.821 ± 0.027	0.609CysThr: 0.609 ± 0.022	0.679CysVal: 0.679 ± 0.025	0.086CysTrp: 0.086 ± 0.008	0.459CysTyr: 0.459 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	2.94AspAla: 2.94 ± 0.056	0.561AspCys: 0.561 ± 0.023	2.815AspAsp: 2.815 ± 0.058	4.368AspGlu: 4.368 ± 0.071	2.737AspPhe: 2.737 ± 0.053	3.533AspGly: 3.533 ± 0.064	0.556AspHis: 0.556 ± 0.02	6.186AspIle: 6.186 ± 0.08	5.567AspLys: 5.567 ± 0.082	4.621AspLeu: 4.621 ± 0.065	1.499AspMet: 1.499 ± 0.039	3.428AspAsn: 3.428 ± 0.055	1.23AspPro: 1.23 ± 0.034	0.823AspGln: 0.823 ± 0.027	1.746AspArg: 1.746 ± 0.041	3.263AspSer: 3.263 ± 0.052	2.622AspThr: 2.622 ± 0.047	3.74AspVal: 3.74 ± 0.053	0.401AspTrp: 0.401 ± 0.017	2.521AspTyr: 2.521 ± 0.049	0.0AspXaa: 0.0 ± 0.0
Glu	4.017GluAla: 4.017 ± 0.066	0.675GluCys: 0.675 ± 0.024	3.981GluAsp: 3.981 ± 0.072	5.811GluGlu: 5.811 ± 0.103	2.789GluPhe: 2.789 ± 0.059	3.431GluGly: 3.431 ± 0.056	0.957GluHis: 0.957 ± 0.027	6.95GluIle: 6.95 ± 0.093	7.48GluLys: 7.48 ± 0.1	6.289GluLeu: 6.289 ± 0.082	1.731GluMet: 1.731 ± 0.042	5.215GluAsn: 5.215 ± 0.083	1.314GluPro: 1.314 ± 0.029	1.772GluGln: 1.772 ± 0.043	2.281GluArg: 2.281 ± 0.047	3.209GluSer: 3.209 ± 0.053	2.949GluThr: 2.949 ± 0.049	4.429GluVal: 4.429 ± 0.06	0.43GluTrp: 0.43 ± 0.02	2.828GluTyr: 2.828 ± 0.061	0.0GluXaa: 0.0 ± 0.0
Phe	2.271PheAla: 2.271 ± 0.048	0.596PheCys: 0.596 ± 0.023	2.583PheAsp: 2.583 ± 0.046	2.704PheGlu: 2.704 ± 0.048	2.055PhePhe: 2.055 ± 0.053	2.901PheGly: 2.901 ± 0.057	0.611PheHis: 0.611 ± 0.023	4.74PheIle: 4.74 ± 0.079	3.99PheLys: 3.99 ± 0.059	3.953PheLeu: 3.953 ± 0.075	1.194PheMet: 1.194 ± 0.033	3.087PheAsn: 3.087 ± 0.049	1.217PhePro: 1.217 ± 0.03	1.07PheGln: 1.07 ± 0.033	1.251PheArg: 1.251 ± 0.033	3.446PheSer: 3.446 ± 0.06	2.329PheThr: 2.329 ± 0.05	2.799PheVal: 2.799 ± 0.057	0.359PheTrp: 0.359 ± 0.019	1.856PheTyr: 1.856 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	3.979GlyAla: 3.979 ± 0.095	0.881GlyCys: 0.881 ± 0.031	3.114GlyAsp: 3.114 ± 0.053	3.948GlyGlu: 3.948 ± 0.061	3.074GlyPhe: 3.074 ± 0.058	4.077GlyGly: 4.077 ± 0.076	0.988GlyHis: 0.988 ± 0.028	6.978GlyIle: 6.978 ± 0.107	5.505GlyLys: 5.505 ± 0.077	5.161GlyLeu: 5.161 ± 0.079	1.73GlyMet: 1.73 ± 0.04	3.47GlyAsn: 3.47 ± 0.069	1.201GlyPro: 1.201 ± 0.037	1.485GlyGln: 1.485 ± 0.04	2.08GlyArg: 2.08 ± 0.044	3.826GlySer: 3.826 ± 0.064	3.636GlyThr: 3.636 ± 0.077	4.42GlyVal: 4.42 ± 0.066	0.501GlyTrp: 0.501 ± 0.026	2.96GlyTyr: 2.96 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	0.704HisAla: 0.704 ± 0.026	0.214HisCys: 0.214 ± 0.014	0.704HisAsp: 0.704 ± 0.023	0.86HisGlu: 0.86 ± 0.023	0.668HisPhe: 0.668 ± 0.024	1.024HisGly: 1.024 ± 0.031	0.299HisHis: 0.299 ± 0.02	1.345HisIle: 1.345 ± 0.034	1.06HisLys: 1.06 ± 0.034	1.129HisLeu: 1.129 ± 0.032	0.343HisMet: 0.343 ± 0.018	0.843HisAsn: 0.843 ± 0.027	0.604HisPro: 0.604 ± 0.025	0.309HisGln: 0.309 ± 0.018	0.509HisArg: 0.509 ± 0.021	0.872HisSer: 0.872 ± 0.029	0.703HisThr: 0.703 ± 0.026	0.79HisVal: 0.79 ± 0.027	0.128HisTrp: 0.128 ± 0.01	0.583HisTyr: 0.583 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.882IleAla: 5.882 ± 0.082	1.342IleCys: 1.342 ± 0.036	5.857IleAsp: 5.857 ± 0.063	6.826IleGlu: 6.826 ± 0.098	4.443IlePhe: 4.443 ± 0.077	6.408IleGly: 6.408 ± 0.096	1.303IleHis: 1.303 ± 0.035	10.082IleIle: 10.082 ± 0.143	9.143IleLys: 9.143 ± 0.101	9.269IleLeu: 9.269 ± 0.116	2.425IleMet: 2.425 ± 0.052	6.84IleAsn: 6.84 ± 0.086	3.182IlePro: 3.182 ± 0.054	2.218IleGln: 2.218 ± 0.043	3.014IleArg: 3.014 ± 0.059	7.388IleSer: 7.388 ± 0.094	5.144IleThr: 5.144 ± 0.077	6.284IleVal: 6.284 ± 0.08	0.588IleTrp: 0.588 ± 0.026	3.77IleTyr: 3.77 ± 0.064	0.0IleXaa: 0.0 ± 0.0
Lys	5.021LysAla: 5.021 ± 0.073	0.915LysCys: 0.915 ± 0.032	5.807LysAsp: 5.807 ± 0.08	7.532LysGlu: 7.532 ± 0.087	3.567LysPhe: 3.567 ± 0.05	4.733LysGly: 4.733 ± 0.069	1.219LysHis: 1.219 ± 0.031	8.933LysIle: 8.933 ± 0.091	8.393LysLys: 8.393 ± 0.105	8.276LysLeu: 8.276 ± 0.095	2.428LysMet: 2.428 ± 0.051	7.065LysAsn: 7.065 ± 0.071	2.071LysPro: 2.071 ± 0.039	2.359LysGln: 2.359 ± 0.047	2.886LysArg: 2.886 ± 0.05	5.309LysSer: 5.309 ± 0.081	4.346LysThr: 4.346 ± 0.073	5.655LysVal: 5.655 ± 0.08	0.638LysTrp: 0.638 ± 0.027	4.171LysTyr: 4.171 ± 0.066	0.0LysXaa: 0.0 ± 0.0
Leu	4.964LeuAla: 4.964 ± 0.077	1.088LeuCys: 1.088 ± 0.033	4.928LeuAsp: 4.928 ± 0.069	5.762LeuGlu: 5.762 ± 0.077	3.727LeuPhe: 3.727 ± 0.07	5.87LeuGly: 5.87 ± 0.087	1.112LeuHis: 1.112 ± 0.032	8.64LeuIle: 8.64 ± 0.11	8.773LeuLys: 8.773 ± 0.1	7.958LeuLeu: 7.958 ± 0.112	2.293LeuMet: 2.293 ± 0.041	6.155LeuAsn: 6.155 ± 0.07	2.759LeuPro: 2.759 ± 0.052	2.283LeuGln: 2.283 ± 0.044	2.981LeuArg: 2.981 ± 0.056	6.72LeuSer: 6.72 ± 0.08	4.618LeuThr: 4.618 ± 0.064	5.154LeuVal: 5.154 ± 0.066	0.583LeuTrp: 0.583 ± 0.025	3.321LeuTyr: 3.321 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	1.643MetAla: 1.643 ± 0.04	0.259MetCys: 0.259 ± 0.014	1.649MetAsp: 1.649 ± 0.039	1.745MetGlu: 1.745 ± 0.039	1.071MetPhe: 1.071 ± 0.034	1.691MetGly: 1.691 ± 0.039	0.377MetHis: 0.377 ± 0.019	2.165MetIle: 2.165 ± 0.047	2.466MetLys: 2.466 ± 0.042	2.408MetLeu: 2.408 ± 0.045	0.571MetMet: 0.571 ± 0.023	1.773MetAsn: 1.773 ± 0.044	0.882MetPro: 0.882 ± 0.029	0.667MetGln: 0.667 ± 0.025	0.813MetArg: 0.813 ± 0.027	1.746MetSer: 1.746 ± 0.039	1.131MetThr: 1.131 ± 0.033	1.466MetVal: 1.466 ± 0.03	0.182MetTrp: 0.182 ± 0.013	0.895MetTyr: 0.895 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	3.087AsnAla: 3.087 ± 0.062	0.745AsnCys: 0.745 ± 0.025	3.257AsnAsp: 3.257 ± 0.061	4.407AsnGlu: 4.407 ± 0.07	2.912AsnPhe: 2.912 ± 0.052	4.066AsnGly: 4.066 ± 0.077	0.801AsnHis: 0.801 ± 0.028	7.278AsnIle: 7.278 ± 0.09	6.411AsnLys: 6.411 ± 0.08	5.837AsnLeu: 5.837 ± 0.069	1.72AsnMet: 1.72 ± 0.039	4.704AsnAsn: 4.704 ± 0.094	2.068AsnPro: 2.068 ± 0.047	1.453AsnGln: 1.453 ± 0.038	1.962AsnArg: 1.962 ± 0.043	4.454AsnSer: 4.454 ± 0.074	3.277AsnThr: 3.277 ± 0.062	4.15AsnVal: 4.15 ± 0.061	0.494AsnTrp: 0.494 ± 0.025	2.896AsnTyr: 2.896 ± 0.058	0.0AsnXaa: 0.0 ± 0.0
Pro	1.41ProAla: 1.41 ± 0.039	0.308ProCys: 0.308 ± 0.016	1.451ProAsp: 1.451 ± 0.039	1.969ProGlu: 1.969 ± 0.045	1.393ProPhe: 1.393 ± 0.033	1.606ProGly: 1.606 ± 0.046	0.449ProHis: 0.449 ± 0.023	2.705ProIle: 2.705 ± 0.049	2.286ProLys: 2.286 ± 0.048	2.442ProLeu: 2.442 ± 0.055	0.745ProMet: 0.745 ± 0.027	1.595ProAsn: 1.595 ± 0.042	0.594ProPro: 0.594 ± 0.023	0.767ProGln: 0.767 ± 0.023	0.774ProArg: 0.774 ± 0.028	1.789ProSer: 1.789 ± 0.044	1.545ProThr: 1.545 ± 0.043	1.967ProVal: 1.967 ± 0.045	0.233ProTrp: 0.233 ± 0.014	1.263ProTyr: 1.263 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	1.37GlnAla: 1.37 ± 0.038	0.28GlnCys: 0.28 ± 0.016	1.253GlnAsp: 1.253 ± 0.034	1.472GlnGlu: 1.472 ± 0.036	1.094GlnPhe: 1.094 ± 0.034	1.491GlnGly: 1.491 ± 0.035	0.323GlnHis: 0.323 ± 0.016	2.367GlnIle: 2.367 ± 0.047	2.155GlnLys: 2.155 ± 0.043	2.122GlnLeu: 2.122 ± 0.044	0.62GlnMet: 0.62 ± 0.025	1.695GlnAsn: 1.695 ± 0.041	0.567GlnPro: 0.567 ± 0.026	0.76GlnGln: 0.76 ± 0.028	0.835GlnArg: 0.835 ± 0.028	1.462GlnSer: 1.462 ± 0.036	1.149GlnThr: 1.149 ± 0.033	1.487GlnVal: 1.487 ± 0.039	0.249GlnTrp: 0.249 ± 0.014	1.131GlnTyr: 1.131 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	1.79ArgAla: 1.79 ± 0.042	0.359ArgCys: 0.359 ± 0.018	1.749ArgAsp: 1.749 ± 0.04	2.599ArgGlu: 2.599 ± 0.054	1.46ArgPhe: 1.46 ± 0.035	1.862ArgGly: 1.862 ± 0.042	0.467ArgHis: 0.467 ± 0.021	3.01ArgIle: 3.01 ± 0.052	2.934ArgLys: 2.934 ± 0.053	2.756ArgLeu: 2.756 ± 0.045	0.822ArgMet: 0.822 ± 0.028	2.072ArgAsn: 2.072 ± 0.044	0.833ArgPro: 0.833 ± 0.026	0.872ArgGln: 0.872 ± 0.029	1.287ArgArg: 1.287 ± 0.038	1.613ArgSer: 1.613 ± 0.034	1.584ArgThr: 1.584 ± 0.039	1.98ArgVal: 1.98 ± 0.042	0.248ArgTrp: 0.248 ± 0.017	1.343ArgTyr: 1.343 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	3.483SerAla: 3.483 ± 0.059	0.653SerCys: 0.653 ± 0.021	3.432SerAsp: 3.432 ± 0.06	4.195SerGlu: 4.195 ± 0.062	3.166SerPhe: 3.166 ± 0.052	4.461SerGly: 4.461 ± 0.073	0.863SerHis: 0.863 ± 0.028	6.933SerIle: 6.933 ± 0.078	6.05SerLys: 6.05 ± 0.072	5.927SerLeu: 5.927 ± 0.088	1.677SerMet: 1.677 ± 0.038	4.319SerAsn: 4.319 ± 0.082	1.652SerPro: 1.652 ± 0.036	1.659SerGln: 1.659 ± 0.043	2.025SerArg: 2.025 ± 0.042	4.898SerSer: 4.898 ± 0.093	3.473SerThr: 3.473 ± 0.063	3.955SerVal: 3.955 ± 0.057	0.445SerTrp: 0.445 ± 0.021	2.659SerTyr: 2.659 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	3.242ThrAla: 3.242 ± 0.077	0.521ThrCys: 0.521 ± 0.022	2.622ThrAsp: 2.622 ± 0.057	3.037ThrGlu: 3.037 ± 0.064	2.276ThrPhe: 2.276 ± 0.043	3.808ThrGly: 3.808 ± 0.088	0.768ThrHis: 0.768 ± 0.028	5.033ThrIle: 5.033 ± 0.078	3.902ThrLys: 3.902 ± 0.059	4.788ThrLeu: 4.788 ± 0.073	1.112ThrMet: 1.112 ± 0.034	2.991ThrAsn: 2.991 ± 0.063	1.739ThrPro: 1.739 ± 0.038	1.187ThrGln: 1.187 ± 0.035	1.459ThrArg: 1.459 ± 0.03	3.498ThrSer: 3.498 ± 0.064	2.785ThrThr: 2.785 ± 0.073	3.54ThrVal: 3.54 ± 0.064	0.363ThrTrp: 0.363 ± 0.021	2.011ThrTyr: 2.011 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	3.846ValAla: 3.846 ± 0.065	0.817ValCys: 0.817 ± 0.027	3.687ValAsp: 3.687 ± 0.063	3.961ValGlu: 3.961 ± 0.066	3.01ValPhe: 3.01 ± 0.055	4.059ValGly: 4.059 ± 0.066	0.892ValHis: 0.892 ± 0.031	6.304ValIle: 6.304 ± 0.072	5.345ValLys: 5.345 ± 0.072	5.854ValLeu: 5.854 ± 0.078	1.626ValMet: 1.626 ± 0.034	3.862ValAsn: 3.862 ± 0.067	2.121ValPro: 2.121 ± 0.048	1.557ValGln: 1.557 ± 0.036	1.886ValArg: 1.886 ± 0.044	4.474ValSer: 4.474 ± 0.067	3.485ValThr: 3.485 ± 0.057	4.427ValVal: 4.427 ± 0.071	0.386ValTrp: 0.386 ± 0.02	2.446ValTyr: 2.446 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.409TrpAla: 0.409 ± 0.02	0.097TrpCys: 0.097 ± 0.008	0.361TrpAsp: 0.361 ± 0.017	0.406TrpGlu: 0.406 ± 0.02	0.36TrpPhe: 0.36 ± 0.022	0.494TrpGly: 0.494 ± 0.023	0.136TrpHis: 0.136 ± 0.01	0.731TrpIle: 0.731 ± 0.024	0.532TrpLys: 0.532 ± 0.023	0.609TrpLeu: 0.609 ± 0.026	0.198TrpMet: 0.198 ± 0.013	0.497TrpAsn: 0.497 ± 0.022	0.169TrpPro: 0.169 ± 0.012	0.252TrpGln: 0.252 ± 0.017	0.249TrpArg: 0.249 ± 0.014	0.484TrpSer: 0.484 ± 0.02	0.347TrpThr: 0.347 ± 0.017	0.411TrpVal: 0.411 ± 0.02	0.092TrpTrp: 0.092 ± 0.009	0.281TrpTyr: 0.281 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.989TyrAla: 1.989 ± 0.041	0.563TyrCys: 0.563 ± 0.025	2.525TyrAsp: 2.525 ± 0.057	2.769TyrGlu: 2.769 ± 0.053	2.084TyrPhe: 2.084 ± 0.047	2.579TyrGly: 2.579 ± 0.057	0.533TyrHis: 0.533 ± 0.025	3.965TyrIle: 3.965 ± 0.061	3.733TyrLys: 3.733 ± 0.057	3.615TyrLeu: 3.615 ± 0.058	1.003TyrMet: 1.003 ± 0.029	2.892TyrAsn: 2.892 ± 0.053	1.256TyrPro: 1.256 ± 0.035	0.808TyrGln: 0.808 ± 0.029	1.441TyrArg: 1.441 ± 0.033	2.948TyrSer: 2.948 ± 0.056	2.035TyrThr: 2.035 ± 0.045	2.54TyrVal: 2.54 ± 0.045	0.305TyrTrp: 0.305 ± 0.017	1.798TyrTyr: 1.798 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3815 proteins (1156905 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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