Amino acid dipepetide frequency for Escherichia phage SH2026Stx1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.038AlaAla: 9.038 ± 0.882
1.378AlaCys: 1.378 ± 0.436
6.228AlaAsp: 6.228 ± 0.674
8.432AlaGlu: 8.432 ± 0.722
3.748AlaPhe: 3.748 ± 0.481
7.881AlaGly: 7.881 ± 1.17
1.598AlaHis: 1.598 ± 0.357
4.354AlaIle: 4.354 ± 0.557
4.354AlaLys: 4.354 ± 0.458
7.771AlaLeu: 7.771 ± 0.94
2.866AlaMet: 2.866 ± 0.497
3.031AlaAsn: 3.031 ± 0.362
2.921AlaPro: 2.921 ± 0.415
4.96AlaGln: 4.96 ± 0.754
5.842AlaArg: 5.842 ± 0.581
5.621AlaSer: 5.621 ± 0.567
5.015AlaThr: 5.015 ± 0.891
6.172AlaVal: 6.172 ± 0.626
1.488AlaTrp: 1.488 ± 0.296
2.48AlaTyr: 2.48 ± 0.351
0.0AlaXaa: 0.0 ± 0.0
Cys
1.102CysAla: 1.102 ± 0.258
0.496CysCys: 0.496 ± 0.191
0.551CysAsp: 0.551 ± 0.193
0.716CysGlu: 0.716 ± 0.217
0.606CysPhe: 0.606 ± 0.201
0.937CysGly: 0.937 ± 0.26
0.276CysHis: 0.276 ± 0.114
0.661CysIle: 0.661 ± 0.25
0.606CysLys: 0.606 ± 0.212
0.661CysLeu: 0.661 ± 0.193
0.22CysMet: 0.22 ± 0.13
0.386CysAsn: 0.386 ± 0.215
0.551CysPro: 0.551 ± 0.168
0.22CysGln: 0.22 ± 0.106
1.102CysArg: 1.102 ± 0.306
0.827CysSer: 0.827 ± 0.253
0.551CysThr: 0.551 ± 0.208
0.827CysVal: 0.827 ± 0.246
0.22CysTrp: 0.22 ± 0.126
0.22CysTyr: 0.22 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
5.952AspAla: 5.952 ± 0.669
0.441AspCys: 0.441 ± 0.171
3.692AspAsp: 3.692 ± 0.425
4.905AspGlu: 4.905 ± 0.597
1.653AspPhe: 1.653 ± 0.321
4.85AspGly: 4.85 ± 0.556
1.102AspHis: 1.102 ± 0.35
3.582AspIle: 3.582 ± 0.366
4.023AspLys: 4.023 ± 0.378
4.299AspLeu: 4.299 ± 0.411
1.653AspMet: 1.653 ± 0.275
2.645AspAsn: 2.645 ± 0.424
2.48AspPro: 2.48 ± 0.391
1.488AspGln: 1.488 ± 0.327
3.252AspArg: 3.252 ± 0.355
3.196AspSer: 3.196 ± 0.532
2.59AspThr: 2.59 ± 0.476
3.913AspVal: 3.913 ± 0.375
0.992AspTrp: 0.992 ± 0.27
1.598AspTyr: 1.598 ± 0.304
0.0AspXaa: 0.0 ± 0.0
Glu
6.393GluAla: 6.393 ± 0.569
0.937GluCys: 0.937 ± 0.243
2.37GluAsp: 2.37 ± 0.267
4.078GluGlu: 4.078 ± 0.551
1.929GluPhe: 1.929 ± 0.347
3.637GluGly: 3.637 ± 0.597
1.268GluHis: 1.268 ± 0.29
3.748GluIle: 3.748 ± 0.486
4.519GluLys: 4.519 ± 0.527
5.897GluLeu: 5.897 ± 0.579
2.204GluMet: 2.204 ± 0.323
2.756GluAsn: 2.756 ± 0.472
1.764GluPro: 1.764 ± 0.342
4.299GluGln: 4.299 ± 0.591
5.621GluArg: 5.621 ± 0.663
4.023GluSer: 4.023 ± 0.429
3.307GluThr: 3.307 ± 0.655
4.519GluVal: 4.519 ± 0.593
0.882GluTrp: 0.882 ± 0.196
1.598GluTyr: 1.598 ± 0.323
0.0GluXaa: 0.0 ± 0.0
Phe
2.756PheAla: 2.756 ± 0.476
0.386PheCys: 0.386 ± 0.138
1.653PheAsp: 1.653 ± 0.293
1.212PheGlu: 1.212 ± 0.335
1.047PhePhe: 1.047 ± 0.303
2.315PheGly: 2.315 ± 0.269
0.606PheHis: 0.606 ± 0.154
2.204PheIle: 2.204 ± 0.37
1.433PheLys: 1.433 ± 0.262
2.094PheLeu: 2.094 ± 0.317
0.827PheMet: 0.827 ± 0.221
1.378PheAsn: 1.378 ± 0.26
1.378PhePro: 1.378 ± 0.232
0.716PheGln: 0.716 ± 0.208
2.425PheArg: 2.425 ± 0.377
2.7PheSer: 2.7 ± 0.387
2.094PheThr: 2.094 ± 0.293
2.149PheVal: 2.149 ± 0.346
0.606PheTrp: 0.606 ± 0.197
1.102PheTyr: 1.102 ± 0.247
0.0PheXaa: 0.0 ± 0.0
Gly
5.787GlyAla: 5.787 ± 1.065
0.716GlyCys: 0.716 ± 0.232
4.629GlyAsp: 4.629 ± 0.87
5.897GlyGlu: 5.897 ± 1.494
2.7GlyPhe: 2.7 ± 0.426
5.511GlyGly: 5.511 ± 0.739
0.882GlyHis: 0.882 ± 0.211
4.133GlyIle: 4.133 ± 0.584
4.684GlyLys: 4.684 ± 0.893
4.684GlyLeu: 4.684 ± 0.433
2.425GlyMet: 2.425 ± 0.344
2.645GlyAsn: 2.645 ± 0.333
3.913GlyPro: 3.913 ± 2.358
2.756GlyGln: 2.756 ± 0.406
4.464GlyArg: 4.464 ± 0.565
4.299GlySer: 4.299 ± 0.527
3.692GlyThr: 3.692 ± 0.496
5.897GlyVal: 5.897 ± 0.527
1.157GlyTrp: 1.157 ± 0.254
2.315GlyTyr: 2.315 ± 0.399
0.0GlyXaa: 0.0 ± 0.0
His
1.874HisAla: 1.874 ± 0.288
0.11HisCys: 0.11 ± 0.072
0.992HisAsp: 0.992 ± 0.246
1.157HisGlu: 1.157 ± 0.316
0.882HisPhe: 0.882 ± 0.269
1.653HisGly: 1.653 ± 0.321
0.386HisHis: 0.386 ± 0.151
0.606HisIle: 0.606 ± 0.194
0.827HisLys: 0.827 ± 0.286
1.378HisLeu: 1.378 ± 0.336
0.331HisMet: 0.331 ± 0.126
1.157HisAsn: 1.157 ± 0.247
1.102HisPro: 1.102 ± 0.399
0.606HisGln: 0.606 ± 0.205
0.827HisArg: 0.827 ± 0.222
1.102HisSer: 1.102 ± 0.272
0.772HisThr: 0.772 ± 0.194
1.268HisVal: 1.268 ± 0.288
0.165HisTrp: 0.165 ± 0.119
0.496HisTyr: 0.496 ± 0.148
0.0HisXaa: 0.0 ± 0.0
Ile
5.18IleAla: 5.18 ± 0.622
1.102IleCys: 1.102 ± 0.287
3.913IleAsp: 3.913 ± 0.427
2.535IleGlu: 2.535 ± 0.384
1.323IlePhe: 1.323 ± 0.264
3.196IleGly: 3.196 ± 0.53
1.102IleHis: 1.102 ± 0.255
2.26IleIle: 2.26 ± 0.431
2.425IleLys: 2.425 ± 0.385
2.976IleLeu: 2.976 ± 0.615
1.268IleMet: 1.268 ± 0.246
3.086IleAsn: 3.086 ± 0.403
2.48IlePro: 2.48 ± 0.315
1.819IleGln: 1.819 ± 0.281
4.96IleArg: 4.96 ± 0.432
3.913IleSer: 3.913 ± 0.527
3.803IleThr: 3.803 ± 0.521
2.315IleVal: 2.315 ± 0.41
0.386IleTrp: 0.386 ± 0.154
1.653IleTyr: 1.653 ± 0.343
0.0IleXaa: 0.0 ± 0.0
Lys
6.007LysAla: 6.007 ± 0.592
0.496LysCys: 0.496 ± 0.222
3.141LysAsp: 3.141 ± 0.4
3.307LysGlu: 3.307 ± 0.402
0.937LysPhe: 0.937 ± 0.191
4.629LysGly: 4.629 ± 1.337
1.157LysHis: 1.157 ± 0.246
3.307LysIle: 3.307 ± 0.462
4.078LysLys: 4.078 ± 0.678
5.291LysLeu: 5.291 ± 0.544
1.874LysMet: 1.874 ± 0.33
2.866LysAsn: 2.866 ± 0.375
2.921LysPro: 2.921 ± 0.392
2.866LysGln: 2.866 ± 0.422
3.637LysArg: 3.637 ± 0.473
2.811LysSer: 2.811 ± 0.464
4.354LysThr: 4.354 ± 0.482
2.7LysVal: 2.7 ± 0.409
0.772LysTrp: 0.772 ± 0.256
1.488LysTyr: 1.488 ± 0.294
0.0LysXaa: 0.0 ± 0.0
Leu
9.259LeuAla: 9.259 ± 0.837
1.268LeuCys: 1.268 ± 0.308
3.968LeuAsp: 3.968 ± 0.544
3.858LeuGlu: 3.858 ± 0.475
2.7LeuPhe: 2.7 ± 0.42
4.905LeuGly: 4.905 ± 0.454
1.268LeuHis: 1.268 ± 0.284
3.803LeuIle: 3.803 ± 0.561
4.85LeuLys: 4.85 ± 0.449
7.495LeuLeu: 7.495 ± 0.718
2.48LeuMet: 2.48 ± 0.44
4.629LeuAsn: 4.629 ± 0.565
4.74LeuPro: 4.74 ± 0.62
2.976LeuGln: 2.976 ± 0.733
5.346LeuArg: 5.346 ± 0.537
6.007LeuSer: 6.007 ± 0.582
5.621LeuThr: 5.621 ± 0.526
4.299LeuVal: 4.299 ± 0.428
0.772LeuTrp: 0.772 ± 0.218
2.204LeuTyr: 2.204 ± 0.401
0.0LeuXaa: 0.0 ± 0.0
Met
3.252MetAla: 3.252 ± 0.4
0.055MetCys: 0.055 ± 0.06
1.488MetAsp: 1.488 ± 0.344
1.212MetGlu: 1.212 ± 0.238
0.551MetPhe: 0.551 ± 0.139
1.488MetGly: 1.488 ± 0.318
0.331MetHis: 0.331 ± 0.102
1.212MetIle: 1.212 ± 0.258
1.929MetLys: 1.929 ± 0.329
2.094MetLeu: 2.094 ± 0.355
0.772MetMet: 0.772 ± 0.188
1.598MetAsn: 1.598 ± 0.375
1.157MetPro: 1.157 ± 0.269
1.543MetGln: 1.543 ± 0.3
2.094MetArg: 2.094 ± 0.286
2.204MetSer: 2.204 ± 0.381
2.039MetThr: 2.039 ± 0.301
1.212MetVal: 1.212 ± 0.337
0.276MetTrp: 0.276 ± 0.134
0.551MetTyr: 0.551 ± 0.187
0.0MetXaa: 0.0 ± 0.0
Asn
4.74AsnAla: 4.74 ± 0.637
0.441AsnCys: 0.441 ± 0.152
2.425AsnAsp: 2.425 ± 0.447
2.921AsnGlu: 2.921 ± 0.43
1.047AsnPhe: 1.047 ± 0.235
3.692AsnGly: 3.692 ± 0.377
0.882AsnHis: 0.882 ± 0.272
2.48AsnIle: 2.48 ± 0.378
2.645AsnLys: 2.645 ± 0.355
3.307AsnLeu: 3.307 ± 0.414
1.102AsnMet: 1.102 ± 0.272
1.929AsnAsn: 1.929 ± 0.415
1.819AsnPro: 1.819 ± 0.27
1.764AsnGln: 1.764 ± 0.313
3.031AsnArg: 3.031 ± 0.421
2.48AsnSer: 2.48 ± 0.545
2.26AsnThr: 2.26 ± 0.417
2.26AsnVal: 2.26 ± 0.39
0.441AsnTrp: 0.441 ± 0.137
1.157AsnTyr: 1.157 ± 0.323
0.0AsnXaa: 0.0 ± 0.0
Pro
3.858ProAla: 3.858 ± 0.675
0.441ProCys: 0.441 ± 0.167
3.913ProAsp: 3.913 ± 0.478
4.74ProGlu: 4.74 ± 0.615
1.268ProPhe: 1.268 ± 0.228
3.086ProGly: 3.086 ± 0.575
0.661ProHis: 0.661 ± 0.151
1.047ProIle: 1.047 ± 0.222
2.921ProLys: 2.921 ± 0.772
3.086ProLeu: 3.086 ± 0.436
0.606ProMet: 0.606 ± 0.172
1.102ProAsn: 1.102 ± 0.275
1.378ProPro: 1.378 ± 0.374
2.315ProGln: 2.315 ± 0.583
2.039ProArg: 2.039 ± 0.38
2.866ProSer: 2.866 ± 0.352
1.874ProThr: 1.874 ± 0.29
4.299ProVal: 4.299 ± 0.46
0.661ProTrp: 0.661 ± 0.2
1.433ProTyr: 1.433 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
4.244GlnAla: 4.244 ± 0.532
0.716GlnCys: 0.716 ± 0.196
2.26GlnAsp: 2.26 ± 0.357
2.811GlnGlu: 2.811 ± 0.408
1.212GlnPhe: 1.212 ± 0.292
3.141GlnGly: 3.141 ± 0.767
0.827GlnHis: 0.827 ± 0.211
2.26GlnIle: 2.26 ± 0.465
3.307GlnLys: 3.307 ± 0.44
3.803GlnLeu: 3.803 ± 0.448
0.992GlnMet: 0.992 ± 0.268
1.488GlnAsn: 1.488 ± 0.332
1.598GlnPro: 1.598 ± 0.359
3.307GlnGln: 3.307 ± 0.875
3.637GlnArg: 3.637 ± 0.548
2.37GlnSer: 2.37 ± 0.326
2.315GlnThr: 2.315 ± 0.379
2.59GlnVal: 2.59 ± 0.503
0.661GlnTrp: 0.661 ± 0.153
1.433GlnTyr: 1.433 ± 0.261
0.0GlnXaa: 0.0 ± 0.0
Arg
4.133ArgAla: 4.133 ± 0.384
0.276ArgCys: 0.276 ± 0.122
4.188ArgAsp: 4.188 ± 0.668
4.464ArgGlu: 4.464 ± 0.711
2.535ArgPhe: 2.535 ± 0.402
4.684ArgGly: 4.684 ± 1.002
1.819ArgHis: 1.819 ± 0.287
3.913ArgIle: 3.913 ± 0.541
4.188ArgLys: 4.188 ± 0.579
6.283ArgLeu: 6.283 ± 0.579
1.874ArgMet: 1.874 ± 0.268
3.582ArgAsn: 3.582 ± 0.368
2.26ArgPro: 2.26 ± 0.374
3.141ArgGln: 3.141 ± 0.453
6.007ArgArg: 6.007 ± 0.651
4.299ArgSer: 4.299 ± 0.436
3.748ArgThr: 3.748 ± 0.483
4.684ArgVal: 4.684 ± 0.565
1.157ArgTrp: 1.157 ± 0.247
2.039ArgTyr: 2.039 ± 0.452
0.0ArgXaa: 0.0 ± 0.0
Ser
5.952SerAla: 5.952 ± 0.565
0.716SerCys: 0.716 ± 0.253
3.637SerAsp: 3.637 ± 0.389
4.629SerGlu: 4.629 ± 0.403
1.488SerPhe: 1.488 ± 0.257
5.952SerGly: 5.952 ± 0.712
0.716SerHis: 0.716 ± 0.227
2.811SerIle: 2.811 ± 0.547
2.866SerLys: 2.866 ± 0.47
7.109SerLeu: 7.109 ± 0.626
1.653SerMet: 1.653 ± 0.373
2.204SerAsn: 2.204 ± 0.358
3.196SerPro: 3.196 ± 0.367
3.196SerGln: 3.196 ± 0.482
3.803SerArg: 3.803 ± 0.564
3.748SerSer: 3.748 ± 0.857
3.527SerThr: 3.527 ± 0.606
3.858SerVal: 3.858 ± 0.487
0.772SerTrp: 0.772 ± 0.206
1.874SerTyr: 1.874 ± 0.372
0.0SerXaa: 0.0 ± 0.0
Thr
4.96ThrAla: 4.96 ± 0.454
0.551ThrCys: 0.551 ± 0.215
3.307ThrAsp: 3.307 ± 0.447
3.692ThrGlu: 3.692 ± 0.333
1.708ThrPhe: 1.708 ± 0.351
5.456ThrGly: 5.456 ± 0.851
1.157ThrHis: 1.157 ± 0.232
3.362ThrIle: 3.362 ± 0.441
3.141ThrLys: 3.141 ± 0.357
5.566ThrLeu: 5.566 ± 0.625
0.772ThrMet: 0.772 ± 0.18
1.819ThrAsn: 1.819 ± 0.278
3.472ThrPro: 3.472 ± 0.344
2.37ThrGln: 2.37 ± 0.465
2.37ThrArg: 2.37 ± 0.304
4.023ThrSer: 4.023 ± 0.676
2.7ThrThr: 2.7 ± 0.477
3.913ThrVal: 3.913 ± 0.529
0.661ThrTrp: 0.661 ± 0.165
1.323ThrTyr: 1.323 ± 0.339
0.0ThrXaa: 0.0 ± 0.0
Val
6.889ValAla: 6.889 ± 0.656
0.827ValCys: 0.827 ± 0.238
3.858ValAsp: 3.858 ± 0.491
3.472ValGlu: 3.472 ± 0.372
1.984ValPhe: 1.984 ± 0.304
3.417ValGly: 3.417 ± 0.512
0.772ValHis: 0.772 ± 0.168
3.968ValIle: 3.968 ± 0.504
3.582ValLys: 3.582 ± 0.416
5.732ValLeu: 5.732 ± 0.783
1.819ValMet: 1.819 ± 0.279
2.811ValAsn: 2.811 ± 0.33
2.149ValPro: 2.149 ± 0.299
2.094ValGln: 2.094 ± 0.371
4.85ValArg: 4.85 ± 0.763
4.684ValSer: 4.684 ± 0.522
3.968ValThr: 3.968 ± 0.584
4.133ValVal: 4.133 ± 0.432
0.992ValTrp: 0.992 ± 0.208
1.874ValTyr: 1.874 ± 0.321
0.0ValXaa: 0.0 ± 0.0
Trp
0.772TrpAla: 0.772 ± 0.197
0.165TrpCys: 0.165 ± 0.094
0.496TrpAsp: 0.496 ± 0.175
0.441TrpGlu: 0.441 ± 0.129
0.772TrpPhe: 0.772 ± 0.187
0.661TrpGly: 0.661 ± 0.162
0.496TrpHis: 0.496 ± 0.169
0.551TrpIle: 0.551 ± 0.177
1.047TrpLys: 1.047 ± 0.229
1.433TrpLeu: 1.433 ± 0.347
0.496TrpMet: 0.496 ± 0.147
0.551TrpAsn: 0.551 ± 0.183
0.937TrpPro: 0.937 ± 0.289
1.047TrpGln: 1.047 ± 0.214
1.323TrpArg: 1.323 ± 0.293
0.827TrpSer: 0.827 ± 0.212
0.441TrpThr: 0.441 ± 0.133
0.827TrpVal: 0.827 ± 0.204
0.331TrpTrp: 0.331 ± 0.143
0.496TrpTyr: 0.496 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.031TyrAla: 3.031 ± 0.381
0.22TyrCys: 0.22 ± 0.122
1.598TyrAsp: 1.598 ± 0.27
1.268TyrGlu: 1.268 ± 0.296
0.992TyrPhe: 0.992 ± 0.208
2.149TyrGly: 2.149 ± 0.397
0.386TyrHis: 0.386 ± 0.139
1.433TyrIle: 1.433 ± 0.386
1.212TyrLys: 1.212 ± 0.36
1.488TyrLeu: 1.488 ± 0.32
0.772TyrMet: 0.772 ± 0.208
1.157TyrAsn: 1.157 ± 0.239
1.378TyrPro: 1.378 ± 0.224
1.488TyrGln: 1.488 ± 0.261
2.645TyrArg: 2.645 ± 0.447
1.708TyrSer: 1.708 ± 0.305
1.653TyrThr: 1.653 ± 0.381
2.039TyrVal: 2.039 ± 0.303
0.716TyrTrp: 0.716 ± 0.177
1.212TyrTyr: 1.212 ± 0.219
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (18146 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski