Pseudomonas phage MR15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3TE09|A0A6M3TE09_9CAUD Putative tail fiber assembly domain protein OS=Pseudomonas phage MR15 OX=2711179 GN=Psm1vBMR15_gp11 PE=4 SV=1
MM1 pKa = 7.44SEE3 pKa = 4.62WINVSDD9 pKa = 5.17KK10 pKa = 11.13LPEE13 pKa = 4.81LDD15 pKa = 3.76TPVWLCMPGNIMVIGEE31 pKa = 4.4RR32 pKa = 11.84GSGTDD37 pKa = 2.7GWLWSACYY45 pKa = 10.21GFYY48 pKa = 10.69FNSSGKK54 pKa = 9.11WDD56 pKa = 3.93ANEE59 pKa = 4.18SDD61 pKa = 4.51ASDD64 pKa = 4.58DD65 pKa = 3.88YY66 pKa = 11.84EE67 pKa = 4.33PTHH70 pKa = 6.43WMPLPQPPTAA80 pKa = 4.73

Molecular weight: 9.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3TE02|A0A6M3TE02_9CAUD Putative site-specific DNA-methyltransferase OS=Pseudomonas phage MR15 OX=2711179 GN=Psm1vBMR15_gp29c PE=4 SV=1
MM1 pKa = 7.13GHH3 pKa = 7.08DD4 pKa = 3.9RR5 pKa = 11.84NNHH8 pKa = 5.0SKK10 pKa = 9.34HH11 pKa = 5.9HH12 pKa = 6.42KK13 pKa = 9.19KK14 pKa = 10.74ASASQSTPYY23 pKa = 10.48FFGRR27 pKa = 11.84CCSFAFRR34 pKa = 11.84LEE36 pKa = 3.92KK37 pKa = 10.48RR38 pKa = 11.84HH39 pKa = 6.1FPNHH43 pKa = 6.94PINFARR49 pKa = 11.84NIPGRR54 pKa = 11.84CRR56 pKa = 11.84CAHH59 pKa = 6.55KK60 pKa = 10.77

Molecular weight: 7.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

16324

30

1344

173.7

19.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.927 ± 0.661

1.317 ± 0.161

5.513 ± 0.218

6.23 ± 0.261

3.241 ± 0.196

7.4 ± 0.28

1.697 ± 0.175

5.134 ± 0.2

5.127 ± 0.295

8.049 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.548 ± 0.167

3.633 ± 0.219

4.386 ± 0.294

4.637 ± 0.365

6.003 ± 0.301

6.573 ± 0.258

5.869 ± 0.254

6.432 ± 0.204

1.476 ± 0.143

2.806 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski