Amino acid dipepetide frequency for Pseudomonas phage MR15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.419AlaAla: 16.419 ± 2.108
1.593AlaCys: 1.593 ± 0.391
6.31AlaAsp: 6.31 ± 0.567
9.251AlaGlu: 9.251 ± 1.424
3.308AlaPhe: 3.308 ± 0.382
8.699AlaGly: 8.699 ± 0.994
1.777AlaHis: 1.777 ± 0.409
4.717AlaIle: 4.717 ± 0.521
6.188AlaLys: 6.188 ± 0.729
10.537AlaLeu: 10.537 ± 1.095
4.411AlaMet: 4.411 ± 0.463
4.105AlaAsn: 4.105 ± 0.496
3.615AlaPro: 3.615 ± 0.567
5.697AlaGln: 5.697 ± 0.87
6.616AlaArg: 6.616 ± 0.668
8.271AlaSer: 8.271 ± 0.845
5.575AlaThr: 5.575 ± 0.675
6.433AlaVal: 6.433 ± 0.678
1.715AlaTrp: 1.715 ± 0.409
4.043AlaTyr: 4.043 ± 0.612
0.0AlaXaa: 0.0 ± 0.0
Cys
1.715CysAla: 1.715 ± 0.355
0.306CysCys: 0.306 ± 0.123
0.613CysAsp: 0.613 ± 0.18
0.245CysGlu: 0.245 ± 0.126
0.245CysPhe: 0.245 ± 0.115
0.98CysGly: 0.98 ± 0.283
0.551CysHis: 0.551 ± 0.177
0.796CysIle: 0.796 ± 0.259
0.49CysLys: 0.49 ± 0.17
1.47CysLeu: 1.47 ± 0.359
0.429CysMet: 0.429 ± 0.146
0.245CysAsn: 0.245 ± 0.111
0.674CysPro: 0.674 ± 0.227
0.123CysGln: 0.123 ± 0.074
0.98CysArg: 0.98 ± 0.242
0.858CysSer: 0.858 ± 0.251
0.674CysThr: 0.674 ± 0.193
1.225CysVal: 1.225 ± 0.241
0.245CysTrp: 0.245 ± 0.138
0.306CysTyr: 0.306 ± 0.152
0.0CysXaa: 0.0 ± 0.0
Asp
6.861AspAla: 6.861 ± 0.862
0.796AspCys: 0.796 ± 0.238
2.634AspAsp: 2.634 ± 0.453
3.982AspGlu: 3.982 ± 0.56
2.022AspPhe: 2.022 ± 0.366
5.207AspGly: 5.207 ± 0.611
0.98AspHis: 0.98 ± 0.295
2.328AspIle: 2.328 ± 0.316
2.267AspLys: 2.267 ± 0.416
4.227AspLeu: 4.227 ± 0.457
1.593AspMet: 1.593 ± 0.291
1.715AspAsn: 1.715 ± 0.273
2.267AspPro: 2.267 ± 0.385
2.818AspGln: 2.818 ± 0.429
3.369AspArg: 3.369 ± 0.474
3.308AspSer: 3.308 ± 0.47
2.634AspThr: 2.634 ± 0.482
3.186AspVal: 3.186 ± 0.444
1.103AspTrp: 1.103 ± 0.216
1.838AspTyr: 1.838 ± 0.334
0.0AspXaa: 0.0 ± 0.0
Glu
8.148GluAla: 8.148 ± 0.785
0.796GluCys: 0.796 ± 0.239
2.634GluAsp: 2.634 ± 0.437
3.186GluGlu: 3.186 ± 0.503
2.573GluPhe: 2.573 ± 0.359
3.369GluGly: 3.369 ± 0.396
0.919GluHis: 0.919 ± 0.233
4.288GluIle: 4.288 ± 0.588
3.492GluLys: 3.492 ± 0.504
6.31GluLeu: 6.31 ± 0.757
1.348GluMet: 1.348 ± 0.274
1.593GluAsn: 1.593 ± 0.306
2.328GluPro: 2.328 ± 0.375
4.288GluGln: 4.288 ± 0.692
4.84GluArg: 4.84 ± 0.749
2.941GluSer: 2.941 ± 0.427
2.634GluThr: 2.634 ± 0.321
3.921GluVal: 3.921 ± 0.409
1.164GluTrp: 1.164 ± 0.278
1.532GluTyr: 1.532 ± 0.391
0.0GluXaa: 0.0 ± 0.0
Phe
4.105PheAla: 4.105 ± 0.457
0.306PheCys: 0.306 ± 0.13
2.267PheAsp: 2.267 ± 0.377
1.777PheGlu: 1.777 ± 0.32
0.735PhePhe: 0.735 ± 0.241
2.573PheGly: 2.573 ± 0.459
0.613PheHis: 0.613 ± 0.161
2.205PheIle: 2.205 ± 0.482
1.287PheLys: 1.287 ± 0.276
2.083PheLeu: 2.083 ± 0.308
0.98PheMet: 0.98 ± 0.224
1.287PheAsn: 1.287 ± 0.301
1.838PhePro: 1.838 ± 0.352
0.796PheGln: 0.796 ± 0.245
1.287PheArg: 1.287 ± 0.26
2.205PheSer: 2.205 ± 0.414
1.96PheThr: 1.96 ± 0.308
2.389PheVal: 2.389 ± 0.399
0.613PheTrp: 0.613 ± 0.205
1.103PheTyr: 1.103 ± 0.211
0.0PheXaa: 0.0 ± 0.0
Gly
6.861GlyAla: 6.861 ± 0.872
1.041GlyCys: 1.041 ± 0.245
3.982GlyAsp: 3.982 ± 0.511
4.656GlyGlu: 4.656 ± 0.438
2.267GlyPhe: 2.267 ± 0.321
6.188GlyGly: 6.188 ± 0.655
1.593GlyHis: 1.593 ± 0.389
3.676GlyIle: 3.676 ± 0.51
4.656GlyLys: 4.656 ± 0.397
6.188GlyLeu: 6.188 ± 0.56
1.593GlyMet: 1.593 ± 0.33
2.818GlyAsn: 2.818 ± 0.642
2.205GlyPro: 2.205 ± 0.355
3.553GlyGln: 3.553 ± 0.455
5.085GlyArg: 5.085 ± 0.684
4.411GlySer: 4.411 ± 0.582
4.84GlyThr: 4.84 ± 0.715
5.146GlyVal: 5.146 ± 0.606
1.041GlyTrp: 1.041 ± 0.258
2.144GlyTyr: 2.144 ± 0.363
0.0GlyXaa: 0.0 ± 0.0
His
1.777HisAla: 1.777 ± 0.291
0.306HisCys: 0.306 ± 0.16
1.103HisAsp: 1.103 ± 0.304
1.164HisGlu: 1.164 ± 0.266
0.368HisPhe: 0.368 ± 0.161
1.103HisGly: 1.103 ± 0.24
0.306HisHis: 0.306 ± 0.145
0.919HisIle: 0.919 ± 0.223
1.041HisLys: 1.041 ± 0.305
1.287HisLeu: 1.287 ± 0.27
0.429HisMet: 0.429 ± 0.152
0.735HisAsn: 0.735 ± 0.198
1.103HisPro: 1.103 ± 0.276
0.735HisGln: 0.735 ± 0.22
0.858HisArg: 0.858 ± 0.244
1.041HisSer: 1.041 ± 0.29
1.103HisThr: 1.103 ± 0.294
0.735HisVal: 0.735 ± 0.21
0.245HisTrp: 0.245 ± 0.146
0.613HisTyr: 0.613 ± 0.232
0.0HisXaa: 0.0 ± 0.0
Ile
6.861IleAla: 6.861 ± 0.72
0.674IleCys: 0.674 ± 0.213
4.043IleAsp: 4.043 ± 0.462
3.982IleGlu: 3.982 ± 0.43
1.103IlePhe: 1.103 ± 0.21
3.247IleGly: 3.247 ± 0.476
0.613IleHis: 0.613 ± 0.152
2.451IleIle: 2.451 ± 0.35
2.573IleLys: 2.573 ± 0.407
2.879IleLeu: 2.879 ± 0.404
0.674IleMet: 0.674 ± 0.195
2.389IleAsn: 2.389 ± 0.407
2.634IlePro: 2.634 ± 0.34
2.022IleGln: 2.022 ± 0.436
2.512IleArg: 2.512 ± 0.295
3.553IleSer: 3.553 ± 0.534
3.308IleThr: 3.308 ± 0.468
3.798IleVal: 3.798 ± 0.48
0.858IleTrp: 0.858 ± 0.245
1.164IleTyr: 1.164 ± 0.29
0.0IleXaa: 0.0 ± 0.0
Lys
7.229LysAla: 7.229 ± 0.731
0.306LysCys: 0.306 ± 0.148
2.634LysAsp: 2.634 ± 0.345
2.573LysGlu: 2.573 ± 0.475
1.715LysPhe: 1.715 ± 0.34
3.247LysGly: 3.247 ± 0.426
0.735LysHis: 0.735 ± 0.228
2.267LysIle: 2.267 ± 0.343
2.573LysLys: 2.573 ± 0.418
4.533LysLeu: 4.533 ± 0.464
1.164LysMet: 1.164 ± 0.269
1.593LysAsn: 1.593 ± 0.275
2.389LysPro: 2.389 ± 0.419
2.083LysGln: 2.083 ± 0.399
3.86LysArg: 3.86 ± 0.551
3.308LysSer: 3.308 ± 0.483
3.186LysThr: 3.186 ± 0.429
3.982LysVal: 3.982 ± 0.579
0.429LysTrp: 0.429 ± 0.15
1.47LysTyr: 1.47 ± 0.356
0.0LysXaa: 0.0 ± 0.0
Leu
10.108LeuAla: 10.108 ± 0.926
0.919LeuCys: 0.919 ± 0.308
4.227LeuAsp: 4.227 ± 0.502
5.391LeuGlu: 5.391 ± 0.499
2.144LeuPhe: 2.144 ± 0.395
5.943LeuGly: 5.943 ± 0.654
1.409LeuHis: 1.409 ± 0.317
3.676LeuIle: 3.676 ± 0.536
4.288LeuLys: 4.288 ± 0.555
6.371LeuLeu: 6.371 ± 0.557
1.47LeuMet: 1.47 ± 0.269
4.043LeuAsn: 4.043 ± 0.377
4.043LeuPro: 4.043 ± 0.636
2.941LeuGln: 2.941 ± 0.461
5.391LeuArg: 5.391 ± 0.517
5.881LeuSer: 5.881 ± 0.577
5.024LeuThr: 5.024 ± 0.676
4.717LeuVal: 4.717 ± 0.485
1.041LeuTrp: 1.041 ± 0.219
1.47LeuTyr: 1.47 ± 0.344
0.0LeuXaa: 0.0 ± 0.0
Met
2.879MetAla: 2.879 ± 0.369
0.245MetCys: 0.245 ± 0.11
1.041MetAsp: 1.041 ± 0.259
1.225MetGlu: 1.225 ± 0.389
0.735MetPhe: 0.735 ± 0.228
2.022MetGly: 2.022 ± 0.325
0.245MetHis: 0.245 ± 0.128
1.409MetIle: 1.409 ± 0.254
1.348MetLys: 1.348 ± 0.29
1.899MetLeu: 1.899 ± 0.331
0.306MetMet: 0.306 ± 0.139
0.98MetAsn: 0.98 ± 0.264
1.777MetPro: 1.777 ± 0.48
1.225MetGln: 1.225 ± 0.241
1.532MetArg: 1.532 ± 0.338
2.389MetSer: 2.389 ± 0.467
2.144MetThr: 2.144 ± 0.366
1.348MetVal: 1.348 ± 0.266
0.245MetTrp: 0.245 ± 0.121
0.49MetTyr: 0.49 ± 0.181
0.0MetXaa: 0.0 ± 0.0
Asn
4.595AsnAla: 4.595 ± 0.705
0.49AsnCys: 0.49 ± 0.167
2.205AsnAsp: 2.205 ± 0.388
1.838AsnGlu: 1.838 ± 0.32
0.796AsnPhe: 0.796 ± 0.192
2.696AsnGly: 2.696 ± 0.415
0.674AsnHis: 0.674 ± 0.21
1.838AsnIle: 1.838 ± 0.315
2.083AsnLys: 2.083 ± 0.377
2.512AsnLeu: 2.512 ± 0.404
0.429AsnMet: 0.429 ± 0.187
1.103AsnAsn: 1.103 ± 0.305
2.389AsnPro: 2.389 ± 0.384
1.47AsnGln: 1.47 ± 0.318
1.409AsnArg: 1.409 ± 0.265
3.308AsnSer: 3.308 ± 0.541
2.512AsnThr: 2.512 ± 0.481
2.389AsnVal: 2.389 ± 0.396
0.735AsnTrp: 0.735 ± 0.247
0.858AsnTyr: 0.858 ± 0.216
0.0AsnXaa: 0.0 ± 0.0
Pro
5.024ProAla: 5.024 ± 0.667
0.613ProCys: 0.613 ± 0.186
2.634ProAsp: 2.634 ± 0.488
2.757ProGlu: 2.757 ± 0.452
1.838ProPhe: 1.838 ± 0.385
3.798ProGly: 3.798 ± 0.507
0.429ProHis: 0.429 ± 0.17
2.512ProIle: 2.512 ± 0.42
2.267ProLys: 2.267 ± 0.442
3.124ProLeu: 3.124 ± 0.383
1.715ProMet: 1.715 ± 0.32
1.654ProAsn: 1.654 ± 0.317
1.593ProPro: 1.593 ± 0.34
1.838ProGln: 1.838 ± 0.399
1.838ProArg: 1.838 ± 0.345
2.879ProSer: 2.879 ± 0.378
2.941ProThr: 2.941 ± 0.464
2.818ProVal: 2.818 ± 0.455
0.551ProTrp: 0.551 ± 0.2
1.041ProTyr: 1.041 ± 0.225
0.0ProXaa: 0.0 ± 0.0
Gln
6.065GlnAla: 6.065 ± 0.943
0.858GlnCys: 0.858 ± 0.244
2.144GlnAsp: 2.144 ± 0.328
1.715GlnGlu: 1.715 ± 0.307
1.838GlnPhe: 1.838 ± 0.322
2.818GlnGly: 2.818 ± 0.487
0.551GlnHis: 0.551 ± 0.209
2.879GlnIle: 2.879 ± 0.397
2.634GlnLys: 2.634 ± 0.605
4.166GlnLeu: 4.166 ± 0.535
1.041GlnMet: 1.041 ± 0.235
1.47GlnAsn: 1.47 ± 0.355
2.389GlnPro: 2.389 ± 0.325
2.451GlnGln: 2.451 ± 0.672
3.431GlnArg: 3.431 ± 0.489
3.124GlnSer: 3.124 ± 0.631
2.144GlnThr: 2.144 ± 0.434
3.002GlnVal: 3.002 ± 0.519
0.306GlnTrp: 0.306 ± 0.131
1.348GlnTyr: 1.348 ± 0.254
0.0GlnXaa: 0.0 ± 0.0
Arg
6.004ArgAla: 6.004 ± 0.624
1.225ArgCys: 1.225 ± 0.278
3.615ArgAsp: 3.615 ± 0.546
3.737ArgGlu: 3.737 ± 0.662
1.96ArgPhe: 1.96 ± 0.345
3.063ArgGly: 3.063 ± 0.519
1.654ArgHis: 1.654 ± 0.364
3.369ArgIle: 3.369 ± 0.437
3.063ArgLys: 3.063 ± 0.523
5.514ArgLeu: 5.514 ± 0.596
2.205ArgMet: 2.205 ± 0.403
2.634ArgAsn: 2.634 ± 0.382
2.328ArgPro: 2.328 ± 0.38
3.369ArgGln: 3.369 ± 0.481
3.86ArgArg: 3.86 ± 0.542
3.308ArgSer: 3.308 ± 0.404
2.696ArgThr: 2.696 ± 0.377
3.186ArgVal: 3.186 ± 0.381
1.225ArgTrp: 1.225 ± 0.292
2.022ArgTyr: 2.022 ± 0.338
0.0ArgXaa: 0.0 ± 0.0
Ser
8.638SerAla: 8.638 ± 1.123
0.613SerCys: 0.613 ± 0.23
2.757SerAsp: 2.757 ± 0.431
3.798SerGlu: 3.798 ± 0.55
2.696SerPhe: 2.696 ± 0.43
5.881SerGly: 5.881 ± 0.814
1.47SerHis: 1.47 ± 0.284
3.186SerIle: 3.186 ± 0.375
2.941SerLys: 2.941 ± 0.403
4.84SerLeu: 4.84 ± 0.507
1.777SerMet: 1.777 ± 0.308
2.328SerAsn: 2.328 ± 0.374
2.144SerPro: 2.144 ± 0.344
3.186SerGln: 3.186 ± 0.525
3.553SerArg: 3.553 ± 0.427
3.798SerSer: 3.798 ± 0.714
4.779SerThr: 4.779 ± 0.578
3.921SerVal: 3.921 ± 0.545
1.164SerTrp: 1.164 ± 0.273
2.267SerTyr: 2.267 ± 0.317
0.0SerXaa: 0.0 ± 0.0
Thr
6.861ThrAla: 6.861 ± 0.758
0.368ThrCys: 0.368 ± 0.135
3.431ThrAsp: 3.431 ± 0.494
3.431ThrGlu: 3.431 ± 0.529
2.267ThrPhe: 2.267 ± 0.448
5.881ThrGly: 5.881 ± 0.511
0.735ThrHis: 0.735 ± 0.259
2.696ThrIle: 2.696 ± 0.388
2.941ThrLys: 2.941 ± 0.476
4.901ThrLeu: 4.901 ± 0.441
1.225ThrMet: 1.225 ± 0.285
2.389ThrAsn: 2.389 ± 0.452
3.063ThrPro: 3.063 ± 0.563
2.144ThrGln: 2.144 ± 0.372
2.818ThrArg: 2.818 ± 0.457
3.553ThrSer: 3.553 ± 0.488
3.431ThrThr: 3.431 ± 0.472
3.921ThrVal: 3.921 ± 0.445
0.919ThrTrp: 0.919 ± 0.225
1.715ThrTyr: 1.715 ± 0.316
0.0ThrXaa: 0.0 ± 0.0
Val
6.004ValAla: 6.004 ± 0.512
1.041ValCys: 1.041 ± 0.263
3.982ValAsp: 3.982 ± 0.497
5.085ValGlu: 5.085 ± 0.671
2.205ValPhe: 2.205 ± 0.324
4.227ValGly: 4.227 ± 0.494
1.103ValHis: 1.103 ± 0.282
3.86ValIle: 3.86 ± 0.489
3.124ValLys: 3.124 ± 0.459
4.288ValLeu: 4.288 ± 0.457
1.593ValMet: 1.593 ± 0.263
2.205ValAsn: 2.205 ± 0.408
2.818ValPro: 2.818 ± 0.441
2.941ValGln: 2.941 ± 0.47
3.615ValArg: 3.615 ± 0.49
4.656ValSer: 4.656 ± 0.526
4.472ValThr: 4.472 ± 0.535
4.901ValVal: 4.901 ± 0.658
0.674ValTrp: 0.674 ± 0.168
1.532ValTyr: 1.532 ± 0.335
0.0ValXaa: 0.0 ± 0.0
Trp
1.103TrpAla: 1.103 ± 0.213
0.245TrpCys: 0.245 ± 0.112
0.858TrpAsp: 0.858 ± 0.255
0.613TrpGlu: 0.613 ± 0.218
0.613TrpPhe: 0.613 ± 0.201
0.98TrpGly: 0.98 ± 0.266
0.245TrpHis: 0.245 ± 0.126
0.735TrpIle: 0.735 ± 0.193
0.858TrpLys: 0.858 ± 0.238
0.858TrpLeu: 0.858 ± 0.28
0.551TrpMet: 0.551 ± 0.175
0.429TrpAsn: 0.429 ± 0.203
0.858TrpPro: 0.858 ± 0.205
0.919TrpGln: 0.919 ± 0.242
0.98TrpArg: 0.98 ± 0.263
1.103TrpSer: 1.103 ± 0.256
1.287TrpThr: 1.287 ± 0.315
1.041TrpVal: 1.041 ± 0.263
0.123TrpTrp: 0.123 ± 0.089
0.368TrpTyr: 0.368 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.022TyrAla: 2.022 ± 0.365
0.429TyrCys: 0.429 ± 0.165
2.022TyrAsp: 2.022 ± 0.368
1.899TyrGlu: 1.899 ± 0.407
0.98TyrPhe: 0.98 ± 0.234
2.083TyrGly: 2.083 ± 0.373
0.368TyrHis: 0.368 ± 0.183
1.532TyrIle: 1.532 ± 0.293
1.164TyrLys: 1.164 ± 0.234
2.512TyrLeu: 2.512 ± 0.367
0.49TyrMet: 0.49 ± 0.179
0.613TyrAsn: 0.613 ± 0.239
1.409TyrPro: 1.409 ± 0.349
1.777TyrGln: 1.777 ± 0.322
2.205TyrArg: 2.205 ± 0.413
1.838TyrSer: 1.838 ± 0.355
1.348TyrThr: 1.348 ± 0.293
2.267TyrVal: 2.267 ± 0.344
0.368TyrTrp: 0.368 ± 0.129
0.735TyrTyr: 0.735 ± 0.202
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (16324 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski