Novipirellula aureliae

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Novipirellula

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5806 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6DHW1|A0A5C6DHW1_9BACT Uncharacterized protein OS=Novipirellula aureliae OX=2527966 GN=Q31b_50690 PE=4 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84NIMCVLTMAFAVAGYY17 pKa = 10.2VEE19 pKa = 4.57SANAAIILSVSPSTGANSLANNSTGQTIDD48 pKa = 3.28LFGYY52 pKa = 10.09SDD54 pKa = 3.74TGALTDD60 pKa = 3.46VLGINLAVQIVGPGGVFEE78 pKa = 5.53GPAGPPSPGGGITFTGISSIWDD100 pKa = 3.25AAGFNAVASGSLSGINGAIGVTDD123 pKa = 5.26DD124 pKa = 5.09DD125 pKa = 4.5FADD128 pKa = 3.6ATTISGDD135 pKa = 3.64ALSPSYY141 pKa = 10.63LGRR144 pKa = 11.84FNIDD148 pKa = 2.87TTGVVDD154 pKa = 4.42QFATLEE160 pKa = 4.13IVAGGSSFVFVGAGTNIPVALSAPYY185 pKa = 9.78QVTAIPEE192 pKa = 4.14PASFAICGLVAGGLCLRR209 pKa = 11.84RR210 pKa = 11.84RR211 pKa = 11.84RR212 pKa = 11.84AVAA215 pKa = 3.43

Molecular weight: 21.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6DF86|A0A5C6DF86_9BACT Uncharacterized protein OS=Novipirellula aureliae OX=2527966 GN=Q31b_54380 PE=4 SV=1
MM1 pKa = 7.15WVAAGWVLFLILLLPAIPGVRR22 pKa = 11.84LRR24 pKa = 11.84PPPAGAFLPMKK35 pKa = 10.43LVTTTLSLVAAGLLLFWPRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84GWLLLNIPIVIVLSIVQWTAWSRR79 pKa = 11.84FPP81 pKa = 5.3

Molecular weight: 9.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5806

0

5806

2067535

29

6247

356.1

39.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.189 ± 0.032

1.183 ± 0.018

6.251 ± 0.034

6.223 ± 0.03

3.782 ± 0.02

7.41 ± 0.04

2.103 ± 0.018

5.486 ± 0.024

3.905 ± 0.033

9.496 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.386 ± 0.019

3.455 ± 0.029

4.94 ± 0.023

3.902 ± 0.024

6.527 ± 0.036

6.977 ± 0.029

5.649 ± 0.046

7.156 ± 0.026

1.461 ± 0.017

2.518 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski