Amino acid dipepetide frequency for Vibrio phage vB_VchM_Kuja

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.81AlaAla: 3.81 ± 0.335
0.71AlaCys: 0.71 ± 0.118
3.164AlaAsp: 3.164 ± 0.283
3.875AlaGlu: 3.875 ± 0.322
2.066AlaPhe: 2.066 ± 0.195
3.401AlaGly: 3.401 ± 0.312
1.076AlaHis: 1.076 ± 0.156
4.025AlaIle: 4.025 ± 0.308
4.456AlaLys: 4.456 ± 0.338
4.714AlaLeu: 4.714 ± 0.347
1.485AlaMet: 1.485 ± 0.168
3.659AlaAsn: 3.659 ± 0.364
1.55AlaPro: 1.55 ± 0.195
1.808AlaGln: 1.808 ± 0.223
2.346AlaArg: 2.346 ± 0.236
3.789AlaSer: 3.789 ± 0.271
3.466AlaThr: 3.466 ± 0.377
4.154AlaVal: 4.154 ± 0.323
0.732AlaTrp: 0.732 ± 0.136
2.282AlaTyr: 2.282 ± 0.221
0.0AlaXaa: 0.0 ± 0.0
Cys
0.538CysAla: 0.538 ± 0.102
0.237CysCys: 0.237 ± 0.081
1.033CysAsp: 1.033 ± 0.146
1.033CysGlu: 1.033 ± 0.157
0.387CysPhe: 0.387 ± 0.086
1.119CysGly: 1.119 ± 0.182
0.301CysHis: 0.301 ± 0.09
0.689CysIle: 0.689 ± 0.117
0.84CysLys: 0.84 ± 0.145
1.098CysLeu: 1.098 ± 0.208
0.215CysMet: 0.215 ± 0.068
0.796CysAsn: 0.796 ± 0.139
0.603CysPro: 0.603 ± 0.108
0.431CysGln: 0.431 ± 0.1
0.603CysArg: 0.603 ± 0.11
1.012CysSer: 1.012 ± 0.201
0.495CysThr: 0.495 ± 0.105
1.162CysVal: 1.162 ± 0.153
0.215CysTrp: 0.215 ± 0.076
0.431CysTyr: 0.431 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
3.38AspAla: 3.38 ± 0.253
0.84AspCys: 0.84 ± 0.138
3.336AspAsp: 3.336 ± 0.271
4.025AspGlu: 4.025 ± 0.333
3.659AspPhe: 3.659 ± 0.248
3.616AspGly: 3.616 ± 0.377
1.055AspHis: 1.055 ± 0.155
4.563AspIle: 4.563 ± 0.3
4.241AspLys: 4.241 ± 0.305
5.252AspLeu: 5.252 ± 0.352
1.808AspMet: 1.808 ± 0.206
3.164AspAsn: 3.164 ± 0.209
2.346AspPro: 2.346 ± 0.24
2.045AspGln: 2.045 ± 0.211
2.26AspArg: 2.26 ± 0.202
2.971AspSer: 2.971 ± 0.279
3.25AspThr: 3.25 ± 0.359
4.757AspVal: 4.757 ± 0.273
0.926AspTrp: 0.926 ± 0.134
2.777AspTyr: 2.777 ± 0.253
0.0AspXaa: 0.0 ± 0.0
Glu
4.434GluAla: 4.434 ± 0.385
0.904GluCys: 0.904 ± 0.144
3.767GluAsp: 3.767 ± 0.316
5.424GluGlu: 5.424 ± 0.408
3.293GluPhe: 3.293 ± 0.269
3.659GluGly: 3.659 ± 0.275
1.787GluHis: 1.787 ± 0.214
4.693GluIle: 4.693 ± 0.344
5.747GluLys: 5.747 ± 0.401
6.565GluLeu: 6.565 ± 0.401
2.11GluMet: 2.11 ± 0.207
4.413GluAsn: 4.413 ± 0.309
2.282GluPro: 2.282 ± 0.223
3.143GluGln: 3.143 ± 0.331
3.487GluArg: 3.487 ± 0.283
3.638GluSer: 3.638 ± 0.262
4.025GluThr: 4.025 ± 0.331
5.123GluVal: 5.123 ± 0.302
0.796GluTrp: 0.796 ± 0.159
3.186GluTyr: 3.186 ± 0.315
0.0GluXaa: 0.0 ± 0.0
Phe
2.174PheAla: 2.174 ± 0.212
0.883PheCys: 0.883 ± 0.15
3.25PheAsp: 3.25 ± 0.277
3.466PheGlu: 3.466 ± 0.251
1.894PhePhe: 1.894 ± 0.187
2.691PheGly: 2.691 ± 0.258
0.538PheHis: 0.538 ± 0.12
3.53PheIle: 3.53 ± 0.296
3.702PheLys: 3.702 ± 0.312
3.25PheLeu: 3.25 ± 0.235
1.485PheMet: 1.485 ± 0.183
2.971PheAsn: 2.971 ± 0.248
1.292PhePro: 1.292 ± 0.137
1.485PheGln: 1.485 ± 0.173
1.937PheArg: 1.937 ± 0.229
3.466PheSer: 3.466 ± 0.35
2.626PheThr: 2.626 ± 0.294
3.53PheVal: 3.53 ± 0.232
0.56PheTrp: 0.56 ± 0.108
2.066PheTyr: 2.066 ± 0.181
0.0PheXaa: 0.0 ± 0.0
Gly
2.906GlyAla: 2.906 ± 0.3
0.538GlyCys: 0.538 ± 0.142
3.767GlyAsp: 3.767 ± 0.252
4.284GlyGlu: 4.284 ± 0.277
3.057GlyPhe: 3.057 ± 0.246
3.853GlyGly: 3.853 ± 0.367
1.055GlyHis: 1.055 ± 0.166
3.466GlyIle: 3.466 ± 0.234
3.573GlyLys: 3.573 ± 0.228
4.994GlyLeu: 4.994 ± 0.279
1.528GlyMet: 1.528 ± 0.216
3.38GlyAsn: 3.38 ± 0.353
0.99GlyPro: 0.99 ± 0.13
2.282GlyGln: 2.282 ± 0.28
2.82GlyArg: 2.82 ± 0.254
4.37GlySer: 4.37 ± 0.363
3.207GlyThr: 3.207 ± 0.339
5.575GlyVal: 5.575 ± 0.392
0.71GlyTrp: 0.71 ± 0.142
2.562GlyTyr: 2.562 ± 0.217
0.0GlyXaa: 0.0 ± 0.0
His
0.71HisAla: 0.71 ± 0.124
0.474HisCys: 0.474 ± 0.087
0.947HisAsp: 0.947 ± 0.134
1.162HisGlu: 1.162 ± 0.148
1.162HisPhe: 1.162 ± 0.16
1.033HisGly: 1.033 ± 0.167
0.344HisHis: 0.344 ± 0.084
1.571HisIle: 1.571 ± 0.179
1.593HisLys: 1.593 ± 0.189
1.485HisLeu: 1.485 ± 0.166
0.517HisMet: 0.517 ± 0.099
1.248HisAsn: 1.248 ± 0.154
0.99HisPro: 0.99 ± 0.125
0.474HisGln: 0.474 ± 0.113
0.667HisArg: 0.667 ± 0.115
1.162HisSer: 1.162 ± 0.157
0.883HisThr: 0.883 ± 0.121
1.098HisVal: 1.098 ± 0.146
0.172HisTrp: 0.172 ± 0.055
0.969HisTyr: 0.969 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
4.09IleAla: 4.09 ± 0.237
0.947IleCys: 0.947 ± 0.16
4.886IleAsp: 4.886 ± 0.303
4.951IleGlu: 4.951 ± 0.333
2.454IlePhe: 2.454 ± 0.237
3.638IleGly: 3.638 ± 0.25
1.162IleHis: 1.162 ± 0.16
4.65IleIle: 4.65 ± 0.366
6.092IleLys: 6.092 ± 0.395
4.843IleLeu: 4.843 ± 0.274
1.636IleMet: 1.636 ± 0.199
4.736IleAsn: 4.736 ± 0.376
2.971IlePro: 2.971 ± 0.248
2.777IleGln: 2.777 ± 0.263
3.272IleArg: 3.272 ± 0.244
4.865IleSer: 4.865 ± 0.293
4.327IleThr: 4.327 ± 0.318
4.499IleVal: 4.499 ± 0.333
0.667IleTrp: 0.667 ± 0.144
2.432IleTyr: 2.432 ± 0.226
0.0IleXaa: 0.0 ± 0.0
Lys
4.241LysAla: 4.241 ± 0.366
0.84LysCys: 0.84 ± 0.165
3.573LysAsp: 3.573 ± 0.279
5.059LysGlu: 5.059 ± 0.401
3.724LysPhe: 3.724 ± 0.289
4.198LysGly: 4.198 ± 0.277
1.873LysHis: 1.873 ± 0.202
5.338LysIle: 5.338 ± 0.388
4.972LysLys: 4.972 ± 0.427
6.565LysLeu: 6.565 ± 0.448
2.002LysMet: 2.002 ± 0.183
4.262LysAsn: 4.262 ± 0.348
3.014LysPro: 3.014 ± 0.26
2.82LysGln: 2.82 ± 0.24
3.638LysArg: 3.638 ± 0.298
3.853LysSer: 3.853 ± 0.269
4.327LysThr: 4.327 ± 0.323
4.305LysVal: 4.305 ± 0.355
1.119LysTrp: 1.119 ± 0.159
3.121LysTyr: 3.121 ± 0.262
0.0LysXaa: 0.0 ± 0.0
Leu
4.305LeuAla: 4.305 ± 0.306
1.076LeuCys: 1.076 ± 0.153
5.381LeuAsp: 5.381 ± 0.356
6.759LeuGlu: 6.759 ± 0.426
3.552LeuPhe: 3.552 ± 0.286
4.822LeuGly: 4.822 ± 0.345
1.313LeuHis: 1.313 ± 0.169
5.317LeuIle: 5.317 ± 0.299
6.63LeuLys: 6.63 ± 0.42
6.695LeuLeu: 6.695 ± 0.454
2.153LeuMet: 2.153 ± 0.248
5.037LeuAsn: 5.037 ± 0.36
3.401LeuPro: 3.401 ± 0.21
2.583LeuGln: 2.583 ± 0.249
4.111LeuArg: 4.111 ± 0.291
5.704LeuSer: 5.704 ± 0.334
5.381LeuThr: 5.381 ± 0.335
5.833LeuVal: 5.833 ± 0.352
0.495LeuTrp: 0.495 ± 0.097
2.841LeuTyr: 2.841 ± 0.207
0.0LeuXaa: 0.0 ± 0.0
Met
1.528MetAla: 1.528 ± 0.199
0.258MetCys: 0.258 ± 0.073
1.378MetAsp: 1.378 ± 0.176
1.593MetGlu: 1.593 ± 0.15
1.507MetPhe: 1.507 ± 0.152
1.507MetGly: 1.507 ± 0.228
0.452MetHis: 0.452 ± 0.095
1.873MetIle: 1.873 ± 0.192
2.11MetLys: 2.11 ± 0.188
2.497MetLeu: 2.497 ± 0.235
0.71MetMet: 0.71 ± 0.124
1.528MetAsn: 1.528 ± 0.215
0.818MetPro: 0.818 ± 0.12
0.904MetGln: 0.904 ± 0.122
1.227MetArg: 1.227 ± 0.16
2.002MetSer: 2.002 ± 0.197
1.421MetThr: 1.421 ± 0.167
1.808MetVal: 1.808 ± 0.181
0.258MetTrp: 0.258 ± 0.086
0.646MetTyr: 0.646 ± 0.113
0.0MetXaa: 0.0 ± 0.0
Asn
4.413AsnAla: 4.413 ± 0.39
0.71AsnCys: 0.71 ± 0.125
3.164AsnAsp: 3.164 ± 0.29
4.585AsnGlu: 4.585 ± 0.306
2.884AsnPhe: 2.884 ± 0.256
4.628AsnGly: 4.628 ± 0.378
1.076AsnHis: 1.076 ± 0.137
4.37AsnIle: 4.37 ± 0.319
4.563AsnLys: 4.563 ± 0.333
4.908AsnLeu: 4.908 ± 0.299
1.528AsnMet: 1.528 ± 0.213
3.832AsnAsn: 3.832 ± 0.293
2.239AsnPro: 2.239 ± 0.21
2.346AsnGln: 2.346 ± 0.214
2.432AsnArg: 2.432 ± 0.25
3.595AsnSer: 3.595 ± 0.284
3.444AsnThr: 3.444 ± 0.285
4.176AsnVal: 4.176 ± 0.347
0.646AsnTrp: 0.646 ± 0.126
2.389AsnTyr: 2.389 ± 0.227
0.0AsnXaa: 0.0 ± 0.0
Pro
1.292ProAla: 1.292 ± 0.19
0.366ProCys: 0.366 ± 0.08
2.26ProAsp: 2.26 ± 0.205
3.078ProGlu: 3.078 ± 0.218
1.916ProPhe: 1.916 ± 0.199
0.775ProGly: 0.775 ± 0.112
0.624ProHis: 0.624 ± 0.126
2.389ProIle: 2.389 ± 0.218
2.562ProLys: 2.562 ± 0.24
2.432ProLeu: 2.432 ± 0.221
0.689ProMet: 0.689 ± 0.151
2.325ProAsn: 2.325 ± 0.246
0.775ProPro: 0.775 ± 0.125
1.399ProGln: 1.399 ± 0.17
1.528ProArg: 1.528 ± 0.21
2.798ProSer: 2.798 ± 0.256
2.131ProThr: 2.131 ± 0.235
2.519ProVal: 2.519 ± 0.254
0.517ProTrp: 0.517 ± 0.098
1.636ProTyr: 1.636 ± 0.181
0.0ProXaa: 0.0 ± 0.0
Gln
2.432GlnAla: 2.432 ± 0.29
0.409GlnCys: 0.409 ± 0.091
1.421GlnAsp: 1.421 ± 0.16
2.648GlnGlu: 2.648 ± 0.231
1.636GlnPhe: 1.636 ± 0.185
1.765GlnGly: 1.765 ± 0.218
0.538GlnHis: 0.538 ± 0.106
3.057GlnIle: 3.057 ± 0.246
2.002GlnLys: 2.002 ± 0.174
3.315GlnLeu: 3.315 ± 0.287
1.033GlnMet: 1.033 ± 0.153
2.368GlnAsn: 2.368 ± 0.259
1.421GlnPro: 1.421 ± 0.203
1.162GlnGln: 1.162 ± 0.151
2.389GlnArg: 2.389 ± 0.206
2.11GlnSer: 2.11 ± 0.197
2.196GlnThr: 2.196 ± 0.319
2.153GlnVal: 2.153 ± 0.205
0.344GlnTrp: 0.344 ± 0.081
1.27GlnTyr: 1.27 ± 0.195
0.0GlnXaa: 0.0 ± 0.0
Arg
2.54ArgAla: 2.54 ± 0.237
0.603ArgCys: 0.603 ± 0.11
3.014ArgAsp: 3.014 ± 0.267
3.035ArgGlu: 3.035 ± 0.224
2.755ArgPhe: 2.755 ± 0.252
2.626ArgGly: 2.626 ± 0.241
0.904ArgHis: 0.904 ± 0.135
3.121ArgIle: 3.121 ± 0.256
2.734ArgLys: 2.734 ± 0.244
3.81ArgLeu: 3.81 ± 0.295
1.292ArgMet: 1.292 ± 0.168
3.186ArgAsn: 3.186 ± 0.271
1.27ArgPro: 1.27 ± 0.189
1.744ArgGln: 1.744 ± 0.197
2.045ArgArg: 2.045 ± 0.219
2.669ArgSer: 2.669 ± 0.214
2.217ArgThr: 2.217 ± 0.201
3.229ArgVal: 3.229 ± 0.26
0.796ArgTrp: 0.796 ± 0.146
1.701ArgTyr: 1.701 ± 0.176
0.0ArgXaa: 0.0 ± 0.0
Ser
3.466SerAla: 3.466 ± 0.314
0.732SerCys: 0.732 ± 0.137
4.047SerAsp: 4.047 ± 0.315
4.241SerGlu: 4.241 ± 0.293
2.841SerPhe: 2.841 ± 0.208
4.779SerGly: 4.779 ± 0.313
1.292SerHis: 1.292 ± 0.157
4.886SerIle: 4.886 ± 0.351
4.779SerLys: 4.779 ± 0.354
5.252SerLeu: 5.252 ± 0.333
1.313SerMet: 1.313 ± 0.154
4.198SerAsn: 4.198 ± 0.334
1.873SerPro: 1.873 ± 0.217
2.11SerGln: 2.11 ± 0.219
2.497SerArg: 2.497 ± 0.236
4.154SerSer: 4.154 ± 0.375
3.702SerThr: 3.702 ± 0.276
4.391SerVal: 4.391 ± 0.324
0.775SerTrp: 0.775 ± 0.126
2.54SerTyr: 2.54 ± 0.273
0.0SerXaa: 0.0 ± 0.0
Thr
3.1ThrAla: 3.1 ± 0.313
0.753ThrCys: 0.753 ± 0.126
3.272ThrAsp: 3.272 ± 0.302
4.348ThrGlu: 4.348 ± 0.386
2.454ThrPhe: 2.454 ± 0.203
3.466ThrGly: 3.466 ± 0.341
1.098ThrHis: 1.098 ± 0.15
4.262ThrIle: 4.262 ± 0.375
4.025ThrLys: 4.025 ± 0.306
4.994ThrLeu: 4.994 ± 0.34
1.356ThrMet: 1.356 ± 0.18
3.659ThrAsn: 3.659 ± 0.327
2.519ThrPro: 2.519 ± 0.216
2.346ThrGln: 2.346 ± 0.245
2.217ThrArg: 2.217 ± 0.194
3.832ThrSer: 3.832 ± 0.357
3.207ThrThr: 3.207 ± 0.407
4.09ThrVal: 4.09 ± 0.313
0.387ThrTrp: 0.387 ± 0.096
2.088ThrTyr: 2.088 ± 0.243
0.0ThrXaa: 0.0 ± 0.0
Val
4.327ValAla: 4.327 ± 0.375
1.012ValCys: 1.012 ± 0.132
5.317ValAsp: 5.317 ± 0.286
5.36ValGlu: 5.36 ± 0.35
3.035ValPhe: 3.035 ± 0.266
4.348ValGly: 4.348 ± 0.336
0.947ValHis: 0.947 ± 0.142
4.714ValIle: 4.714 ± 0.325
4.865ValLys: 4.865 ± 0.304
6.092ValLeu: 6.092 ± 0.406
1.701ValMet: 1.701 ± 0.168
4.391ValAsn: 4.391 ± 0.247
2.131ValPro: 2.131 ± 0.206
2.045ValGln: 2.045 ± 0.192
3.143ValArg: 3.143 ± 0.297
4.994ValSer: 4.994 ± 0.314
4.176ValThr: 4.176 ± 0.331
5.102ValVal: 5.102 ± 0.328
0.883ValTrp: 0.883 ± 0.136
2.949ValTyr: 2.949 ± 0.287
0.0ValXaa: 0.0 ± 0.0
Trp
0.538TrpAla: 0.538 ± 0.123
0.28TrpCys: 0.28 ± 0.076
0.84TrpAsp: 0.84 ± 0.14
0.71TrpGlu: 0.71 ± 0.125
0.56TrpPhe: 0.56 ± 0.118
0.753TrpGly: 0.753 ± 0.118
0.301TrpHis: 0.301 ± 0.088
0.624TrpIle: 0.624 ± 0.142
0.775TrpLys: 0.775 ± 0.159
1.292TrpLeu: 1.292 ± 0.166
0.215TrpMet: 0.215 ± 0.058
0.538TrpAsn: 0.538 ± 0.119
0.194TrpPro: 0.194 ± 0.07
0.431TrpGln: 0.431 ± 0.106
0.753TrpArg: 0.753 ± 0.121
0.581TrpSer: 0.581 ± 0.114
0.581TrpThr: 0.581 ± 0.122
0.99TrpVal: 0.99 ± 0.165
0.129TrpTrp: 0.129 ± 0.049
0.366TrpTyr: 0.366 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.346TyrAla: 2.346 ± 0.205
0.667TyrCys: 0.667 ± 0.133
2.454TyrAsp: 2.454 ± 0.23
2.841TyrGlu: 2.841 ± 0.262
1.98TyrPhe: 1.98 ± 0.198
2.239TyrGly: 2.239 ± 0.215
0.926TyrHis: 0.926 ± 0.166
2.755TyrIle: 2.755 ± 0.235
2.583TyrLys: 2.583 ± 0.259
3.336TyrLeu: 3.336 ± 0.301
1.227TyrMet: 1.227 ± 0.161
2.239TyrAsn: 2.239 ± 0.179
1.335TyrPro: 1.335 ± 0.141
1.335TyrGln: 1.335 ± 0.154
1.916TyrArg: 1.916 ± 0.222
2.303TyrSer: 2.303 ± 0.223
2.411TyrThr: 2.411 ± 0.24
3.035TyrVal: 3.035 ± 0.212
0.323TyrTrp: 0.323 ± 0.086
1.442TyrTyr: 1.442 ± 0.183
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 186 proteins (46457 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski