Amino acid dipepetide frequency for gamma proteobacterium BDW918

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.631AlaAla: 11.631 ± 0.151	1.198AlaCys: 1.198 ± 0.034	6.17AlaAsp: 6.17 ± 0.087	6.911AlaGlu: 6.911 ± 0.09	3.447AlaPhe: 3.447 ± 0.056	7.895AlaGly: 7.895 ± 0.115	1.93AlaHis: 1.93 ± 0.048	5.998AlaIle: 5.998 ± 0.075	4.804AlaLys: 4.804 ± 0.079	11.305AlaLeu: 11.305 ± 0.129	2.95AlaMet: 2.95 ± 0.056	3.77AlaAsn: 3.77 ± 0.06	3.893AlaPro: 3.893 ± 0.062	4.21AlaGln: 4.21 ± 0.057	4.984AlaArg: 4.984 ± 0.064	6.503AlaSer: 6.503 ± 0.081	4.82AlaThr: 4.82 ± 0.08	7.196AlaVal: 7.196 ± 0.083	1.166AlaTrp: 1.166 ± 0.033	2.658AlaTyr: 2.658 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	1.078CysAla: 1.078 ± 0.033	0.152CysCys: 0.152 ± 0.011	0.683CysAsp: 0.683 ± 0.025	0.667CysGlu: 0.667 ± 0.025	0.437CysPhe: 0.437 ± 0.018	1.014CysGly: 1.014 ± 0.029	0.351CysHis: 0.351 ± 0.017	0.578CysIle: 0.578 ± 0.023	0.417CysLys: 0.417 ± 0.019	1.049CysLeu: 1.049 ± 0.035	0.237CysMet: 0.237 ± 0.014	0.371CysAsn: 0.371 ± 0.016	0.529CysPro: 0.529 ± 0.024	0.451CysGln: 0.451 ± 0.019	0.66CysArg: 0.66 ± 0.025	0.665CysSer: 0.665 ± 0.026	0.505CysThr: 0.505 ± 0.018	0.741CysVal: 0.741 ± 0.026	0.146CysTrp: 0.146 ± 0.011	0.328CysTyr: 0.328 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	5.691AspAla: 5.691 ± 0.099	0.626AspCys: 0.626 ± 0.023	3.435AspAsp: 3.435 ± 0.07	3.686AspGlu: 3.686 ± 0.057	2.466AspPhe: 2.466 ± 0.05	4.634AspGly: 4.634 ± 0.089	1.156AspHis: 1.156 ± 0.032	3.964AspIle: 3.964 ± 0.059	2.433AspLys: 2.433 ± 0.049	5.652AspLeu: 5.652 ± 0.074	1.346AspMet: 1.346 ± 0.032	2.196AspAsn: 2.196 ± 0.047	2.488AspPro: 2.488 ± 0.058	2.085AspGln: 2.085 ± 0.046	2.9AspArg: 2.9 ± 0.05	3.454AspSer: 3.454 ± 0.069	2.907AspThr: 2.907 ± 0.069	3.993AspVal: 3.993 ± 0.065	0.906AspTrp: 0.906 ± 0.025	2.114AspTyr: 2.114 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	5.944GluAla: 5.944 ± 0.075	0.518GluCys: 0.518 ± 0.019	2.788GluAsp: 2.788 ± 0.056	3.287GluGlu: 3.287 ± 0.053	2.33GluPhe: 2.33 ± 0.047	3.682GluGly: 3.682 ± 0.055	1.468GluHis: 1.468 ± 0.038	3.664GluIle: 3.664 ± 0.06	2.711GluLys: 2.711 ± 0.054	7.1GluLeu: 7.1 ± 0.08	1.518GluMet: 1.518 ± 0.033	2.221GluAsn: 2.221 ± 0.043	2.054GluPro: 2.054 ± 0.037	3.218GluGln: 3.218 ± 0.06	3.751GluArg: 3.751 ± 0.057	3.331GluSer: 3.331 ± 0.057	2.783GluThr: 2.783 ± 0.052	3.961GluVal: 3.961 ± 0.071	0.729GluTrp: 0.729 ± 0.024	1.68GluTyr: 1.68 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	3.824PheAla: 3.824 ± 0.058	0.498PheCys: 0.498 ± 0.023	2.66PheAsp: 2.66 ± 0.055	2.206PheGlu: 2.206 ± 0.047	1.512PhePhe: 1.512 ± 0.038	3.176PheGly: 3.176 ± 0.06	0.789PheHis: 0.789 ± 0.027	2.323PheIle: 2.323 ± 0.047	1.58PheLys: 1.58 ± 0.044	3.211PheLeu: 3.211 ± 0.058	0.918PheMet: 0.918 ± 0.03	1.65PheAsn: 1.65 ± 0.041	1.423PhePro: 1.423 ± 0.037	1.191PheGln: 1.191 ± 0.029	1.824PheArg: 1.824 ± 0.039	3.094PheSer: 3.094 ± 0.063	2.204PheThr: 2.204 ± 0.061	2.532PheVal: 2.532 ± 0.047	0.491PheTrp: 0.491 ± 0.021	1.269PheTyr: 1.269 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	6.705GlyAla: 6.705 ± 0.091	0.85GlyCys: 0.85 ± 0.026	4.498GlyAsp: 4.498 ± 0.075	4.545GlyGlu: 4.545 ± 0.066	3.364GlyPhe: 3.364 ± 0.06	6.141GlyGly: 6.141 ± 0.093	1.597GlyHis: 1.597 ± 0.034	4.721GlyIle: 4.721 ± 0.071	3.532GlyLys: 3.532 ± 0.058	7.893GlyLeu: 7.893 ± 0.094	2.114GlyMet: 2.114 ± 0.044	2.77GlyAsn: 2.77 ± 0.065	2.304GlyPro: 2.304 ± 0.043	2.75GlyGln: 2.75 ± 0.048	4.149GlyArg: 4.149 ± 0.064	4.753GlySer: 4.753 ± 0.07	3.447GlyThr: 3.447 ± 0.085	5.737GlyVal: 5.737 ± 0.095	0.981GlyTrp: 0.981 ± 0.033	2.372GlyTyr: 2.372 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	1.846HisAla: 1.846 ± 0.041	0.425HisCys: 0.425 ± 0.021	1.068HisAsp: 1.068 ± 0.035	0.999HisGlu: 0.999 ± 0.03	1.024HisPhe: 1.024 ± 0.03	1.688HisGly: 1.688 ± 0.039	0.712HisHis: 0.712 ± 0.031	1.379HisIle: 1.379 ± 0.029	0.779HisLys: 0.779 ± 0.027	2.124HisLeu: 2.124 ± 0.047	0.51HisMet: 0.51 ± 0.022	0.749HisAsn: 0.749 ± 0.027	1.183HisPro: 1.183 ± 0.033	0.903HisGln: 0.903 ± 0.032	1.323HisArg: 1.323 ± 0.035	1.441HisSer: 1.441 ± 0.033	1.003HisThr: 1.003 ± 0.031	1.171HisVal: 1.171 ± 0.036	0.379HisTrp: 0.379 ± 0.018	0.872HisTyr: 0.872 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.971IleAla: 6.971 ± 0.07	0.699IleCys: 0.699 ± 0.024	4.043IleAsp: 4.043 ± 0.068	3.874IleGlu: 3.874 ± 0.055	1.965IlePhe: 1.965 ± 0.043	4.381IleGly: 4.381 ± 0.063	1.233IleHis: 1.233 ± 0.033	3.183IleIle: 3.183 ± 0.055	2.437IleLys: 2.437 ± 0.047	4.811IleLeu: 4.811 ± 0.066	1.14IleMet: 1.14 ± 0.032	2.689IleAsn: 2.689 ± 0.053	2.681IlePro: 2.681 ± 0.052	1.907IleGln: 1.907 ± 0.041	3.074IleArg: 3.074 ± 0.053	4.356IleSer: 4.356 ± 0.071	3.11IleThr: 3.11 ± 0.062	3.828IleVal: 3.828 ± 0.061	0.558IleTrp: 0.558 ± 0.02	1.515IleTyr: 1.515 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	4.378LysAla: 4.378 ± 0.074	0.31LysCys: 0.31 ± 0.018	2.238LysAsp: 2.238 ± 0.043	2.226LysGlu: 2.226 ± 0.053	1.195LysPhe: 1.195 ± 0.034	2.718LysGly: 2.718 ± 0.049	0.961LysHis: 0.961 ± 0.03	2.602LysIle: 2.602 ± 0.053	1.98LysLys: 1.98 ± 0.052	4.478LysLeu: 4.478 ± 0.064	1.152LysMet: 1.152 ± 0.031	1.639LysAsn: 1.639 ± 0.036	2.183LysPro: 2.183 ± 0.043	1.969LysGln: 1.969 ± 0.042	2.577LysArg: 2.577 ± 0.05	2.613LysSer: 2.613 ± 0.049	2.382LysThr: 2.382 ± 0.045	2.995LysVal: 2.995 ± 0.058	0.456LysTrp: 0.456 ± 0.021	1.102LysTyr: 1.102 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	12.06LeuAla: 12.06 ± 0.119	1.24LeuCys: 1.24 ± 0.035	6.077LeuAsp: 6.077 ± 0.078	5.636LeuGlu: 5.636 ± 0.07	3.795LeuPhe: 3.795 ± 0.058	7.837LeuGly: 7.837 ± 0.098	2.123LeuHis: 2.123 ± 0.045	5.646LeuIle: 5.646 ± 0.086	4.459LeuLys: 4.459 ± 0.069	11.109LeuLeu: 11.109 ± 0.136	2.444LeuMet: 2.444 ± 0.048	4.227LeuAsn: 4.227 ± 0.067	5.16LeuPro: 5.16 ± 0.075	4.032LeuGln: 4.032 ± 0.07	6.354LeuArg: 6.354 ± 0.086	8.072LeuSer: 8.072 ± 0.097	4.853LeuThr: 4.853 ± 0.062	6.548LeuVal: 6.548 ± 0.079	1.19LeuTrp: 1.19 ± 0.035	2.512LeuTyr: 2.512 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	2.762MetAla: 2.762 ± 0.051	0.21MetCys: 0.21 ± 0.012	1.245MetAsp: 1.245 ± 0.033	1.13MetGlu: 1.13 ± 0.031	0.733MetPhe: 0.733 ± 0.028	1.715MetGly: 1.715 ± 0.04	0.497MetHis: 0.497 ± 0.021	1.314MetIle: 1.314 ± 0.037	1.134MetLys: 1.134 ± 0.029	2.67MetLeu: 2.67 ± 0.053	0.663MetMet: 0.663 ± 0.023	0.98MetAsn: 0.98 ± 0.03	1.329MetPro: 1.329 ± 0.038	1.051MetGln: 1.051 ± 0.029	1.357MetArg: 1.357 ± 0.034	1.872MetSer: 1.872 ± 0.038	1.412MetThr: 1.412 ± 0.034	1.583MetVal: 1.583 ± 0.037	0.199MetTrp: 0.199 ± 0.012	0.469MetTyr: 0.469 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.786AsnAla: 3.786 ± 0.06	0.457AsnCys: 0.457 ± 0.019	2.123AsnAsp: 2.123 ± 0.048	1.978AsnGlu: 1.978 ± 0.042	1.453AsnPhe: 1.453 ± 0.032	2.899AsnGly: 2.899 ± 0.067	0.776AsnHis: 0.776 ± 0.021	2.588AsnIle: 2.588 ± 0.048	1.584AsnLys: 1.584 ± 0.034	3.496AsnLeu: 3.496 ± 0.051	0.875AsnMet: 0.875 ± 0.024	1.572AsnAsn: 1.572 ± 0.042	2.131AsnPro: 2.131 ± 0.046	1.375AsnGln: 1.375 ± 0.037	1.994AsnArg: 1.994 ± 0.043	2.558AsnSer: 2.558 ± 0.051	2.304AsnThr: 2.304 ± 0.045	2.253AsnVal: 2.253 ± 0.042	0.557AsnTrp: 0.557 ± 0.025	1.243AsnTyr: 1.243 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	4.472ProAla: 4.472 ± 0.068	0.363ProCys: 0.363 ± 0.018	2.64ProAsp: 2.64 ± 0.047	3.342ProGlu: 3.342 ± 0.056	1.574ProPhe: 1.574 ± 0.043	3.109ProGly: 3.109 ± 0.052	0.822ProHis: 0.822 ± 0.026	2.315ProIle: 2.315 ± 0.044	1.791ProLys: 1.791 ± 0.044	4.538ProLeu: 4.538 ± 0.069	1.042ProMet: 1.042 ± 0.029	1.587ProAsn: 1.587 ± 0.034	1.607ProPro: 1.607 ± 0.042	1.686ProGln: 1.686 ± 0.033	2.042ProArg: 2.042 ± 0.04	2.888ProSer: 2.888 ± 0.046	2.001ProThr: 2.001 ± 0.046	3.362ProVal: 3.362 ± 0.059	0.537ProTrp: 0.537 ± 0.022	1.203ProTyr: 1.203 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	4.353GlnAla: 4.353 ± 0.067	0.429GlnCys: 0.429 ± 0.018	1.919GlnAsp: 1.919 ± 0.037	1.953GlnGlu: 1.953 ± 0.044	1.576GlnPhe: 1.576 ± 0.037	2.725GlnGly: 2.725 ± 0.046	1.077GlnHis: 1.077 ± 0.034	2.193GlnIle: 2.193 ± 0.04	1.529GlnLys: 1.529 ± 0.043	4.983GlnLeu: 4.983 ± 0.078	0.934GlnMet: 0.934 ± 0.03	1.403GlnAsn: 1.403 ± 0.034	1.504GlnPro: 1.504 ± 0.033	2.493GlnGln: 2.493 ± 0.058	3.081GlnArg: 3.081 ± 0.056	2.526GlnSer: 2.526 ± 0.048	1.921GlnThr: 1.921 ± 0.034	2.484GlnVal: 2.484 ± 0.049	0.683GlnTrp: 0.683 ± 0.024	1.179GlnTyr: 1.179 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	4.824ArgAla: 4.824 ± 0.071	0.64ArgCys: 0.64 ± 0.024	3.286ArgAsp: 3.286 ± 0.054	3.524ArgGlu: 3.524 ± 0.056	2.431ArgPhe: 2.431 ± 0.045	3.713ArgGly: 3.713 ± 0.06	1.437ArgHis: 1.437 ± 0.036	3.284ArgIle: 3.284 ± 0.054	2.414ArgLys: 2.414 ± 0.046	6.149ArgLeu: 6.149 ± 0.077	1.313ArgMet: 1.313 ± 0.031	2.07ArgAsn: 2.07 ± 0.038	2.107ArgPro: 2.107 ± 0.039	2.632ArgGln: 2.632 ± 0.05	3.635ArgArg: 3.635 ± 0.063	3.589ArgSer: 3.589 ± 0.058	2.086ArgThr: 2.086 ± 0.043	3.661ArgVal: 3.661 ± 0.062	0.88ArgTrp: 0.88 ± 0.026	2.173ArgTyr: 2.173 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	7.058SerAla: 7.058 ± 0.076	0.685SerCys: 0.685 ± 0.024	4.087SerAsp: 4.087 ± 0.058	3.889SerGlu: 3.889 ± 0.06	2.671SerPhe: 2.671 ± 0.065	5.819SerGly: 5.819 ± 0.069	1.418SerHis: 1.418 ± 0.033	3.6SerIle: 3.6 ± 0.075	2.657SerLys: 2.657 ± 0.053	7.127SerLeu: 7.127 ± 0.099	1.476SerMet: 1.476 ± 0.036	2.354SerAsn: 2.354 ± 0.053	2.952SerPro: 2.952 ± 0.057	2.615SerGln: 2.615 ± 0.047	3.58SerArg: 3.58 ± 0.054	4.401SerSer: 4.401 ± 0.071	3.112SerThr: 3.112 ± 0.054	4.466SerVal: 4.466 ± 0.072	0.835SerTrp: 0.835 ± 0.025	1.976SerTyr: 1.976 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	5.402ThrAla: 5.402 ± 0.073	0.361ThrCys: 0.361 ± 0.017	2.695ThrAsp: 2.695 ± 0.054	2.914ThrGlu: 2.914 ± 0.052	1.665ThrPhe: 1.665 ± 0.042	4.291ThrGly: 4.291 ± 0.07	0.936ThrHis: 0.936 ± 0.033	2.769ThrIle: 2.769 ± 0.062	1.655ThrLys: 1.655 ± 0.037	5.591ThrLeu: 5.591 ± 0.073	1.026ThrMet: 1.026 ± 0.028	1.534ThrAsn: 1.534 ± 0.04	2.765ThrPro: 2.765 ± 0.056	1.726ThrGln: 1.726 ± 0.038	2.366ThrArg: 2.366 ± 0.05	3.008ThrSer: 3.008 ± 0.065	2.642ThrThr: 2.642 ± 0.063	3.863ThrVal: 3.863 ± 0.112	0.552ThrTrp: 0.552 ± 0.021	1.219ThrTyr: 1.219 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	7.2ValAla: 7.2 ± 0.087	0.821ValCys: 0.821 ± 0.027	4.285ValAsp: 4.285 ± 0.065	3.953ValGlu: 3.953 ± 0.053	2.847ValPhe: 2.847 ± 0.052	4.717ValGly: 4.717 ± 0.08	1.296ValHis: 1.296 ± 0.036	4.129ValIle: 4.129 ± 0.061	2.777ValLys: 2.777 ± 0.05	7.166ValLeu: 7.166 ± 0.082	1.746ValMet: 1.746 ± 0.043	2.826ValAsn: 2.826 ± 0.049	2.761ValPro: 2.761 ± 0.056	2.156ValGln: 2.156 ± 0.05	3.266ValArg: 3.266 ± 0.054	4.912ValSer: 4.912 ± 0.072	3.504ValThr: 3.504 ± 0.069	5.216ValVal: 5.216 ± 0.078	0.726ValTrp: 0.726 ± 0.024	1.783ValTyr: 1.783 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	0.982TrpAla: 0.982 ± 0.035	0.165TrpCys: 0.165 ± 0.012	0.634TrpAsp: 0.634 ± 0.025	0.56TrpGlu: 0.56 ± 0.023	0.536TrpPhe: 0.536 ± 0.022	0.822TrpGly: 0.822 ± 0.025	0.344TrpHis: 0.344 ± 0.019	0.575TrpIle: 0.575 ± 0.023	0.409TrpLys: 0.409 ± 0.018	1.696TrpLeu: 1.696 ± 0.042	0.322TrpMet: 0.322 ± 0.016	0.423TrpAsn: 0.423 ± 0.021	0.558TrpPro: 0.558 ± 0.022	1.001TrpGln: 1.001 ± 0.031	0.914TrpArg: 0.914 ± 0.025	0.75TrpSer: 0.75 ± 0.023	0.515TrpThr: 0.515 ± 0.022	0.847TrpVal: 0.847 ± 0.029	0.181TrpTrp: 0.181 ± 0.012	0.365TrpTyr: 0.365 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.471TyrAla: 2.471 ± 0.05	0.414TyrCys: 0.414 ± 0.018	1.558TyrAsp: 1.558 ± 0.039	1.457TyrGlu: 1.457 ± 0.034	1.357TyrPhe: 1.357 ± 0.04	2.248TyrGly: 2.248 ± 0.045	0.655TyrHis: 0.655 ± 0.024	1.631TyrIle: 1.631 ± 0.043	1.072TyrLys: 1.072 ± 0.029	3.224TyrLeu: 3.224 ± 0.058	0.597TyrMet: 0.597 ± 0.022	0.97TyrAsn: 0.97 ± 0.025	1.308TyrPro: 1.308 ± 0.035	1.495TyrGln: 1.495 ± 0.037	2.016TyrArg: 2.016 ± 0.043	1.992TyrSer: 1.992 ± 0.046	1.468TyrThr: 1.468 ± 0.041	1.649TyrVal: 1.649 ± 0.032	0.447TyrTrp: 0.447 ± 0.02	0.961TyrTyr: 0.961 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3794 proteins (1259801 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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