Aspergillus ustus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 706 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C1E646|A0A0C1E646_ASPUT C6 zinc finger domain-containing protein OS=Aspergillus ustus OX=40382 GN=HK57_00612 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84FSVLAFIALTASVMASDD19 pKa = 5.54DD20 pKa = 4.13PGPSPTEE27 pKa = 4.06SIGCEE32 pKa = 3.46PHH34 pKa = 6.72GDD36 pKa = 3.33HH37 pKa = 6.46WHH39 pKa = 7.11CDD41 pKa = 3.34APATSTLATTTSSTSEE57 pKa = 3.89SDD59 pKa = 3.43EE60 pKa = 4.85DD61 pKa = 4.46DD62 pKa = 4.06SSVTPGPSPTEE73 pKa = 4.12SIGCEE78 pKa = 3.46PHH80 pKa = 6.57GDD82 pKa = 3.25HH83 pKa = 6.39WHH85 pKa = 6.9CEE87 pKa = 3.79AAATDD92 pKa = 3.49VDD94 pKa = 4.34EE95 pKa = 5.13EE96 pKa = 4.82DD97 pKa = 4.01EE98 pKa = 4.6SVTPGPSPTEE108 pKa = 4.12SIGCEE113 pKa = 3.46PHH115 pKa = 6.72GDD117 pKa = 3.33HH118 pKa = 6.46WHH120 pKa = 7.11CDD122 pKa = 3.36APATTTTDD130 pKa = 3.12SDD132 pKa = 4.43SEE134 pKa = 4.64SSTSAAADD142 pKa = 3.69EE143 pKa = 5.27DD144 pKa = 4.56SSSVTPPPSPTEE156 pKa = 4.04SVGCEE161 pKa = 3.43PHH163 pKa = 6.8GDD165 pKa = 3.13HH166 pKa = 6.47WHH168 pKa = 6.95CDD170 pKa = 3.55GPRR173 pKa = 11.84EE174 pKa = 4.0TDD176 pKa = 3.03EE177 pKa = 4.27ATEE180 pKa = 4.22SSSNATSTTEE190 pKa = 3.46EE191 pKa = 4.23GAAEE195 pKa = 4.0EE196 pKa = 4.41TGDD199 pKa = 4.76DD200 pKa = 3.79EE201 pKa = 4.82DD202 pKa = 3.91AAGIVGVKK210 pKa = 10.27LSGVVAVAVAAAAALLL226 pKa = 4.0

Molecular weight: 22.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C1E790|A0A0C1E790_ASPUT Integral membrane protein OS=Aspergillus ustus OX=40382 GN=HK57_00165 PE=4 SV=1
MM1 pKa = 7.59SRR3 pKa = 11.84GLSALHH9 pKa = 6.39SLLPTVEE16 pKa = 4.03KK17 pKa = 10.75KK18 pKa = 9.88KK19 pKa = 10.68AKK21 pKa = 9.93KK22 pKa = 9.45PRR24 pKa = 11.84PLHH27 pKa = 6.07TIGTSKK33 pKa = 10.69KK34 pKa = 9.32NPIRR38 pKa = 11.84QDD40 pKa = 3.42EE41 pKa = 4.3PPIQCRR47 pKa = 11.84YY48 pKa = 6.47NTHH51 pKa = 5.66AHH53 pKa = 3.93VHH55 pKa = 5.63IPINPSPSSRR65 pKa = 11.84ASTRR69 pKa = 11.84FLLSLRR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84PRR80 pKa = 11.84RR81 pKa = 11.84PARR84 pKa = 11.84LPRR87 pKa = 11.84SRR89 pKa = 11.84AHH91 pKa = 7.07LPGTLPRR98 pKa = 11.84PIKK101 pKa = 10.23ARR103 pKa = 11.84HH104 pKa = 5.91LRR106 pKa = 11.84AQYY109 pKa = 9.63PSTLIVHH116 pKa = 7.3RR117 pKa = 11.84GNAHH121 pKa = 6.0NVTDD125 pKa = 3.98VLACLTVPNSSVRR138 pKa = 11.84QLVSAITFTIGNKK151 pKa = 9.38PDD153 pKa = 3.63LKK155 pKa = 11.19KK156 pKa = 10.38MGEE159 pKa = 4.21GDD161 pKa = 3.83PLVCQKK167 pKa = 10.62GMAVLLEE174 pKa = 4.28ALSVLRR180 pKa = 11.84RR181 pKa = 11.84DD182 pKa = 3.72WGVHH186 pKa = 4.73GQPLLCVVSTTGISEE201 pKa = 4.17TRR203 pKa = 11.84DD204 pKa = 3.16IPLLFYY210 pKa = 10.44PLYY213 pKa = 9.04HH214 pKa = 6.62TLLAVPHH221 pKa = 6.75RR222 pKa = 11.84DD223 pKa = 3.27KK224 pKa = 11.39KK225 pKa = 10.85VMEE228 pKa = 4.48GLLVASGEE236 pKa = 4.17RR237 pKa = 11.84FVLVRR242 pKa = 11.84PSLLWDD248 pKa = 3.51GEE250 pKa = 4.15RR251 pKa = 11.84RR252 pKa = 11.84KK253 pKa = 10.86GKK255 pKa = 10.51GKK257 pKa = 9.96GNGVVRR263 pKa = 11.84VGIADD268 pKa = 3.27ARR270 pKa = 11.84TGVVEE275 pKa = 4.28RR276 pKa = 11.84KK277 pKa = 9.18EE278 pKa = 3.82VGYY281 pKa = 8.78TISRR285 pKa = 11.84EE286 pKa = 4.13AVGGWMFEE294 pKa = 3.89HH295 pKa = 7.31LILRR299 pKa = 11.84AGEE302 pKa = 3.95KK303 pKa = 10.76GLVFEE308 pKa = 5.05EE309 pKa = 4.73KK310 pKa = 10.4AVSLTWW316 pKa = 3.56

Molecular weight: 35.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

706

0

706

453076

70

6946

641.8

70.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.096 ± 0.079

1.396 ± 0.028

5.406 ± 0.046

5.64 ± 0.059

3.925 ± 0.042

7.189 ± 0.063

2.555 ± 0.028

5.417 ± 0.055

3.673 ± 0.046

9.866 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.072 ± 0.028

3.353 ± 0.035

5.507 ± 0.062

3.834 ± 0.043

5.881 ± 0.053

7.884 ± 0.08

6.073 ± 0.047

6.771 ± 0.054

1.593 ± 0.026

2.869 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski