Amino acid dipepetide frequency for Aspergillus ustus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.477AlaAla: 9.477 ± 0.202
1.461AlaCys: 1.461 ± 0.065
4.394AlaAsp: 4.394 ± 0.11
4.856AlaGlu: 4.856 ± 0.123
3.633AlaPhe: 3.633 ± 0.093
6.765AlaGly: 6.765 ± 0.17
2.267AlaHis: 2.267 ± 0.072
5.134AlaIle: 5.134 ± 0.1
3.114AlaLys: 3.114 ± 0.094
9.131AlaLeu: 9.131 ± 0.149
2.22AlaMet: 2.22 ± 0.06
2.944AlaAsn: 2.944 ± 0.079
4.099AlaPro: 4.099 ± 0.106
3.368AlaGln: 3.368 ± 0.084
5.174AlaArg: 5.174 ± 0.131
6.986AlaSer: 6.986 ± 0.127
5.524AlaThr: 5.524 ± 0.113
6.308AlaVal: 6.308 ± 0.127
1.432AlaTrp: 1.432 ± 0.069
2.671AlaTyr: 2.671 ± 0.072
0.0AlaXaa: 0.0 ± 0.0
Cys
1.146CysAla: 1.146 ± 0.056
0.26CysCys: 0.26 ± 0.03
0.768CysAsp: 0.768 ± 0.053
0.656CysGlu: 0.656 ± 0.039
0.671CysPhe: 0.671 ± 0.042
0.934CysGly: 0.934 ± 0.047
0.369CysHis: 0.369 ± 0.029
0.905CysIle: 0.905 ± 0.038
0.45CysLys: 0.45 ± 0.032
1.549CysLeu: 1.549 ± 0.07
0.283CysMet: 0.283 ± 0.023
0.448CysAsn: 0.448 ± 0.032
0.662CysPro: 0.662 ± 0.038
0.543CysGln: 0.543 ± 0.033
0.889CysArg: 0.889 ± 0.049
1.04CysSer: 1.04 ± 0.058
0.814CysThr: 0.814 ± 0.045
1.046CysVal: 1.046 ± 0.05
0.179CysTrp: 0.179 ± 0.023
0.344CysTyr: 0.344 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.842AspAla: 4.842 ± 0.111
0.605AspCys: 0.605 ± 0.038
3.169AspAsp: 3.169 ± 0.105
3.536AspGlu: 3.536 ± 0.102
2.002AspPhe: 2.002 ± 0.067
4.026AspGly: 4.026 ± 0.101
1.216AspHis: 1.216 ± 0.052
3.428AspIle: 3.428 ± 0.111
1.922AspLys: 1.922 ± 0.065
5.352AspLeu: 5.352 ± 0.124
1.07AspMet: 1.07 ± 0.051
1.629AspAsn: 1.629 ± 0.063
3.297AspPro: 3.297 ± 0.079
1.739AspGln: 1.739 ± 0.073
3.066AspArg: 3.066 ± 0.101
3.927AspSer: 3.927 ± 0.108
2.997AspThr: 2.997 ± 0.084
3.772AspVal: 3.772 ± 0.1
0.865AspTrp: 0.865 ± 0.045
1.598AspTyr: 1.598 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
5.187GluAla: 5.187 ± 0.118
0.779GluCys: 0.779 ± 0.041
3.178GluAsp: 3.178 ± 0.083
4.063GluGlu: 4.063 ± 0.127
1.832GluPhe: 1.832 ± 0.064
3.701GluGly: 3.701 ± 0.104
1.344GluHis: 1.344 ± 0.054
3.342GluIle: 3.342 ± 0.088
2.755GluLys: 2.755 ± 0.092
5.149GluLeu: 5.149 ± 0.114
1.349GluMet: 1.349 ± 0.059
2.006GluAsn: 2.006 ± 0.075
2.373GluPro: 2.373 ± 0.076
2.24GluGln: 2.24 ± 0.088
3.573GluArg: 3.573 ± 0.109
3.999GluSer: 3.999 ± 0.092
3.58GluThr: 3.58 ± 0.098
3.399GluVal: 3.399 ± 0.09
0.859GluTrp: 0.859 ± 0.045
1.695GluTyr: 1.695 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
3.355PheAla: 3.355 ± 0.09
0.523PheCys: 0.523 ± 0.034
2.34PheAsp: 2.34 ± 0.076
2.251PheGlu: 2.251 ± 0.074
1.803PhePhe: 1.803 ± 0.068
3.059PheGly: 3.059 ± 0.084
1.035PheHis: 1.035 ± 0.05
2.095PheIle: 2.095 ± 0.077
1.254PheLys: 1.254 ± 0.058
3.761PheLeu: 3.761 ± 0.104
0.766PheMet: 0.766 ± 0.039
1.587PheAsn: 1.587 ± 0.063
1.958PhePro: 1.958 ± 0.068
1.492PheGln: 1.492 ± 0.058
2.061PheArg: 2.061 ± 0.072
3.13PheSer: 3.13 ± 0.098
2.238PheThr: 2.238 ± 0.067
2.582PheVal: 2.582 ± 0.08
0.81PheTrp: 0.81 ± 0.05
1.15PheTyr: 1.15 ± 0.053
0.0PheXaa: 0.0 ± 0.0
Gly
6.006GlyAla: 6.006 ± 0.143
1.176GlyCys: 1.176 ± 0.054
3.512GlyAsp: 3.512 ± 0.091
3.695GlyGlu: 3.695 ± 0.089
3.037GlyPhe: 3.037 ± 0.115
5.836GlyGly: 5.836 ± 0.157
1.9GlyHis: 1.9 ± 0.069
4.074GlyIle: 4.074 ± 0.097
2.854GlyLys: 2.854 ± 0.083
6.957GlyLeu: 6.957 ± 0.133
1.578GlyMet: 1.578 ± 0.063
2.183GlyAsn: 2.183 ± 0.074
3.52GlyPro: 3.52 ± 0.103
2.743GlyGln: 2.743 ± 0.083
4.156GlyArg: 4.156 ± 0.088
5.582GlySer: 5.582 ± 0.135
4.242GlyThr: 4.242 ± 0.11
5.348GlyVal: 5.348 ± 0.12
1.223GlyTrp: 1.223 ± 0.056
2.265GlyTyr: 2.265 ± 0.075
0.0GlyXaa: 0.0 ± 0.0
His
2.306HisAla: 2.306 ± 0.073
0.338HisCys: 0.338 ± 0.027
1.34HisAsp: 1.34 ± 0.056
1.331HisGlu: 1.331 ± 0.056
0.995HisPhe: 0.995 ± 0.047
1.903HisGly: 1.903 ± 0.066
0.87HisHis: 0.87 ± 0.053
1.386HisIle: 1.386 ± 0.053
0.744HisLys: 0.744 ± 0.039
2.567HisLeu: 2.567 ± 0.068
0.516HisMet: 0.516 ± 0.037
0.797HisAsn: 0.797 ± 0.042
1.682HisPro: 1.682 ± 0.059
0.976HisGln: 0.976 ± 0.05
1.666HisArg: 1.666 ± 0.061
1.993HisSer: 1.993 ± 0.077
1.432HisThr: 1.432 ± 0.053
1.583HisVal: 1.583 ± 0.059
0.411HisTrp: 0.411 ± 0.029
0.713HisTyr: 0.713 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
5.231IleAla: 5.231 ± 0.135
0.907IleCys: 0.907 ± 0.056
2.86IleAsp: 2.86 ± 0.085
3.013IleGlu: 3.013 ± 0.095
2.207IlePhe: 2.207 ± 0.082
3.796IleGly: 3.796 ± 0.102
1.492IleHis: 1.492 ± 0.059
2.832IleIle: 2.832 ± 0.104
1.876IleLys: 1.876 ± 0.059
5.319IleLeu: 5.319 ± 0.125
1.029IleMet: 1.029 ± 0.047
1.702IleAsn: 1.702 ± 0.064
3.271IlePro: 3.271 ± 0.091
2.075IleGln: 2.075 ± 0.08
3.052IleArg: 3.052 ± 0.082
4.288IleSer: 4.288 ± 0.101
3.2IleThr: 3.2 ± 0.098
3.712IleVal: 3.712 ± 0.098
0.792IleTrp: 0.792 ± 0.04
1.514IleTyr: 1.514 ± 0.057
0.0IleXaa: 0.0 ± 0.0
Lys
3.39LysAla: 3.39 ± 0.089
0.406LysCys: 0.406 ± 0.035
2.086LysAsp: 2.086 ± 0.062
2.24LysGlu: 2.24 ± 0.081
1.13LysPhe: 1.13 ± 0.053
2.514LysGly: 2.514 ± 0.088
0.909LysHis: 0.909 ± 0.034
1.927LysIle: 1.927 ± 0.067
1.702LysLys: 1.702 ± 0.082
3.584LysLeu: 3.584 ± 0.09
0.733LysMet: 0.733 ± 0.044
1.203LysAsn: 1.203 ± 0.056
2.042LysPro: 2.042 ± 0.072
1.397LysGln: 1.397 ± 0.057
2.253LysArg: 2.253 ± 0.072
2.649LysSer: 2.649 ± 0.087
2.295LysThr: 2.295 ± 0.069
2.545LysVal: 2.545 ± 0.07
0.58LysTrp: 0.58 ± 0.038
1.148LysTyr: 1.148 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
9.588LeuAla: 9.588 ± 0.185
1.448LeuCys: 1.448 ± 0.067
5.922LeuAsp: 5.922 ± 0.112
5.626LeuGlu: 5.626 ± 0.143
3.682LeuPhe: 3.682 ± 0.1
6.924LeuGly: 6.924 ± 0.114
2.71LeuHis: 2.71 ± 0.083
4.399LeuIle: 4.399 ± 0.115
3.388LeuLys: 3.388 ± 0.086
9.797LeuLeu: 9.797 ± 0.194
1.839LeuMet: 1.839 ± 0.062
3.205LeuAsn: 3.205 ± 0.089
5.88LeuPro: 5.88 ± 0.12
4.3LeuGln: 4.3 ± 0.125
6.27LeuArg: 6.27 ± 0.12
8.019LeuSer: 8.019 ± 0.136
5.335LeuThr: 5.335 ± 0.116
6.524LeuVal: 6.524 ± 0.148
1.309LeuTrp: 1.309 ± 0.06
2.498LeuTyr: 2.498 ± 0.08
0.0LeuXaa: 0.0 ± 0.0
Met
2.196MetAla: 2.196 ± 0.066
0.267MetCys: 0.267 ± 0.025
1.081MetAsp: 1.081 ± 0.049
1.192MetGlu: 1.192 ± 0.049
0.766MetPhe: 0.766 ± 0.05
1.366MetGly: 1.366 ± 0.05
0.541MetHis: 0.541 ± 0.036
1.053MetIle: 1.053 ± 0.056
0.755MetLys: 0.755 ± 0.04
2.07MetLeu: 2.07 ± 0.075
0.536MetMet: 0.536 ± 0.042
0.737MetAsn: 0.737 ± 0.041
0.962MetPro: 0.962 ± 0.037
0.854MetGln: 0.854 ± 0.047
1.157MetArg: 1.157 ± 0.047
1.629MetSer: 1.629 ± 0.058
1.355MetThr: 1.355 ± 0.046
1.459MetVal: 1.459 ± 0.052
0.254MetTrp: 0.254 ± 0.024
0.49MetTyr: 0.49 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
2.955AsnAla: 2.955 ± 0.084
0.435AsnCys: 0.435 ± 0.028
1.607AsnAsp: 1.607 ± 0.056
1.761AsnGlu: 1.761 ± 0.078
1.298AsnPhe: 1.298 ± 0.049
2.655AsnGly: 2.655 ± 0.087
0.812AsnHis: 0.812 ± 0.044
2.119AsnIle: 2.119 ± 0.078
1.132AsnLys: 1.132 ± 0.057
3.132AsnLeu: 3.132 ± 0.087
0.77AsnMet: 0.77 ± 0.042
1.355AsnAsn: 1.355 ± 0.067
2.309AsnPro: 2.309 ± 0.075
1.13AsnGln: 1.13 ± 0.055
1.834AsnArg: 1.834 ± 0.058
2.423AsnSer: 2.423 ± 0.082
1.969AsnThr: 1.969 ± 0.065
2.251AsnVal: 2.251 ± 0.067
0.585AsnTrp: 0.585 ± 0.034
0.995AsnTyr: 0.995 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
4.743ProAla: 4.743 ± 0.114
0.611ProCys: 0.611 ± 0.042
3.207ProAsp: 3.207 ± 0.093
3.437ProGlu: 3.437 ± 0.103
2.161ProPhe: 2.161 ± 0.064
3.9ProGly: 3.9 ± 0.122
1.298ProHis: 1.298 ± 0.054
2.794ProIle: 2.794 ± 0.068
2.004ProLys: 2.004 ± 0.074
5.125ProLeu: 5.125 ± 0.102
0.94ProMet: 0.94 ± 0.037
1.834ProAsn: 1.834 ± 0.07
3.58ProPro: 3.58 ± 0.136
1.949ProGln: 1.949 ± 0.073
2.878ProArg: 2.878 ± 0.075
5.123ProSer: 5.123 ± 0.133
3.481ProThr: 3.481 ± 0.099
3.73ProVal: 3.73 ± 0.084
0.865ProTrp: 0.865 ± 0.037
1.406ProTyr: 1.406 ± 0.06
0.0ProXaa: 0.0 ± 0.0
Gln
3.361GlnAla: 3.361 ± 0.096
0.53GlnCys: 0.53 ± 0.035
2.044GlnAsp: 2.044 ± 0.069
2.09GlnGlu: 2.09 ± 0.067
1.397GlnPhe: 1.397 ± 0.046
2.468GlnGly: 2.468 ± 0.073
1.176GlnHis: 1.176 ± 0.055
1.927GlnIle: 1.927 ± 0.063
1.362GlnLys: 1.362 ± 0.051
3.721GlnLeu: 3.721 ± 0.113
0.768GlnMet: 0.768 ± 0.04
1.282GlnAsn: 1.282 ± 0.046
2.123GlnPro: 2.123 ± 0.078
1.911GlnGln: 1.911 ± 0.088
2.604GlnArg: 2.604 ± 0.067
2.827GlnSer: 2.827 ± 0.103
2.39GlnThr: 2.39 ± 0.073
2.441GlnVal: 2.441 ± 0.086
0.695GlnTrp: 0.695 ± 0.039
1.218GlnTyr: 1.218 ± 0.061
0.0GlnXaa: 0.0 ± 0.0
Arg
5.006ArgAla: 5.006 ± 0.127
0.77ArgCys: 0.77 ± 0.047
3.286ArgAsp: 3.286 ± 0.097
3.443ArgGlu: 3.443 ± 0.097
2.421ArgPhe: 2.421 ± 0.067
3.746ArgGly: 3.746 ± 0.103
1.525ArgHis: 1.525 ± 0.051
3.15ArgIle: 3.15 ± 0.086
2.461ArgLys: 2.461 ± 0.078
6.112ArgLeu: 6.112 ± 0.129
1.267ArgMet: 1.267 ± 0.062
1.925ArgAsn: 1.925 ± 0.06
2.801ArgPro: 2.801 ± 0.078
2.388ArgGln: 2.388 ± 0.088
4.216ArgArg: 4.216 ± 0.122
4.185ArgSer: 4.185 ± 0.098
3.269ArgThr: 3.269 ± 0.094
3.922ArgVal: 3.922 ± 0.083
1.108ArgTrp: 1.108 ± 0.058
1.814ArgTyr: 1.814 ± 0.068
0.0ArgXaa: 0.0 ± 0.0
Ser
6.392SerAla: 6.392 ± 0.113
0.989SerCys: 0.989 ± 0.044
3.953SerAsp: 3.953 ± 0.108
3.915SerGlu: 3.915 ± 0.104
3.072SerPhe: 3.072 ± 0.093
5.357SerGly: 5.357 ± 0.102
1.85SerHis: 1.85 ± 0.066
4.412SerIle: 4.412 ± 0.138
2.852SerLys: 2.852 ± 0.09
8.052SerLeu: 8.052 ± 0.162
1.591SerMet: 1.591 ± 0.067
2.713SerAsn: 2.713 ± 0.082
4.681SerPro: 4.681 ± 0.116
2.922SerGln: 2.922 ± 0.089
4.416SerArg: 4.416 ± 0.102
7.906SerSer: 7.906 ± 0.218
5.266SerThr: 5.266 ± 0.138
5.114SerVal: 5.114 ± 0.125
1.289SerTrp: 1.289 ± 0.054
2.095SerTyr: 2.095 ± 0.071
0.0SerXaa: 0.0 ± 0.0
Thr
5.652ThrAla: 5.652 ± 0.101
0.744ThrCys: 0.744 ± 0.044
2.827ThrAsp: 2.827 ± 0.081
3.125ThrGlu: 3.125 ± 0.086
2.459ThrPhe: 2.459 ± 0.076
4.922ThrGly: 4.922 ± 0.108
1.324ThrHis: 1.324 ± 0.051
3.275ThrIle: 3.275 ± 0.094
1.953ThrLys: 1.953 ± 0.07
5.871ThrLeu: 5.871 ± 0.138
1.123ThrMet: 1.123 ± 0.05
1.964ThrAsn: 1.964 ± 0.069
3.858ThrPro: 3.858 ± 0.112
2.112ThrGln: 2.112 ± 0.071
3.055ThrArg: 3.055 ± 0.074
4.973ThrSer: 4.973 ± 0.123
4.397ThrThr: 4.397 ± 0.131
4.302ThrVal: 4.302 ± 0.11
1.004ThrTrp: 1.004 ± 0.046
1.786ThrTyr: 1.786 ± 0.065
0.0ThrXaa: 0.0 ± 0.0
Val
6.156ValAla: 6.156 ± 0.114
1.057ValCys: 1.057 ± 0.057
4.03ValAsp: 4.03 ± 0.094
3.933ValGlu: 3.933 ± 0.1
2.845ValPhe: 2.845 ± 0.077
4.836ValGly: 4.836 ± 0.107
1.56ValHis: 1.56 ± 0.065
3.512ValIle: 3.512 ± 0.092
2.47ValLys: 2.47 ± 0.076
6.802ValLeu: 6.802 ± 0.14
1.287ValMet: 1.287 ± 0.055
2.317ValAsn: 2.317 ± 0.073
3.587ValPro: 3.587 ± 0.098
2.609ValGln: 2.609 ± 0.085
3.929ValArg: 3.929 ± 0.092
5.11ValSer: 5.11 ± 0.131
3.865ValThr: 3.865 ± 0.093
4.979ValVal: 4.979 ± 0.128
0.839ValTrp: 0.839 ± 0.044
1.986ValTyr: 1.986 ± 0.071
0.0ValXaa: 0.0 ± 0.0
Trp
1.419TrpAla: 1.419 ± 0.071
0.219TrpCys: 0.219 ± 0.021
0.923TrpAsp: 0.923 ± 0.044
0.801TrpGlu: 0.801 ± 0.045
0.563TrpPhe: 0.563 ± 0.036
1.0TrpGly: 1.0 ± 0.045
0.397TrpHis: 0.397 ± 0.029
0.859TrpIle: 0.859 ± 0.047
0.788TrpLys: 0.788 ± 0.038
1.56TrpLeu: 1.56 ± 0.061
0.43TrpMet: 0.43 ± 0.032
0.638TrpAsn: 0.638 ± 0.033
0.766TrpPro: 0.766 ± 0.046
0.574TrpGln: 0.574 ± 0.037
0.984TrpArg: 0.984 ± 0.045
1.123TrpSer: 1.123 ± 0.056
1.17TrpThr: 1.17 ± 0.054
0.951TrpVal: 0.951 ± 0.054
0.289TrpTrp: 0.289 ± 0.027
0.472TrpTyr: 0.472 ± 0.033
0.002TrpXaa: 0.002 ± 0.002
Tyr
2.551TyrAla: 2.551 ± 0.065
0.43TyrCys: 0.43 ± 0.036
1.532TyrAsp: 1.532 ± 0.063
1.439TyrGlu: 1.439 ± 0.059
1.276TyrPhe: 1.276 ± 0.051
2.181TyrGly: 2.181 ± 0.066
0.951TyrHis: 0.951 ± 0.045
1.549TyrIle: 1.549 ± 0.056
0.898TyrLys: 0.898 ± 0.044
3.048TyrLeu: 3.048 ± 0.087
0.622TyrMet: 0.622 ± 0.04
1.059TyrAsn: 1.059 ± 0.048
1.616TyrPro: 1.616 ± 0.059
1.015TyrGln: 1.015 ± 0.052
1.576TyrArg: 1.576 ± 0.063
1.927TyrSer: 1.927 ± 0.071
1.907TyrThr: 1.907 ± 0.06
1.739TyrVal: 1.739 ± 0.057
0.541TyrTrp: 0.541 ± 0.038
0.834TyrTyr: 0.834 ± 0.05
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.002XaaArg: 0.002 ± 0.002
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.011XaaXaa: 0.011 ± 0.01
Statistics based on 706 proteins (453076 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski