Amino acid dipepetide frequency for Streptococcus phage phiNJ2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.9AlaAla: 3.9 ± 1.042
0.254AlaCys: 0.254 ± 0.143
5.002AlaAsp: 5.002 ± 0.955
6.359AlaGlu: 6.359 ± 0.942
2.628AlaPhe: 2.628 ± 0.672
5.256AlaGly: 5.256 ± 0.776
0.678AlaHis: 0.678 ± 0.191
6.274AlaIle: 6.274 ± 1.011
6.189AlaLys: 6.189 ± 0.732
6.613AlaLeu: 6.613 ± 1.299
2.12AlaMet: 2.12 ± 0.57
5.087AlaAsn: 5.087 ± 0.747
2.543AlaPro: 2.543 ± 0.355
2.883AlaGln: 2.883 ± 0.54
1.95AlaArg: 1.95 ± 0.395
4.154AlaSer: 4.154 ± 1.058
4.578AlaThr: 4.578 ± 0.688
4.493AlaVal: 4.493 ± 0.788
1.102AlaTrp: 1.102 ± 0.275
2.798AlaTyr: 2.798 ± 0.538
0.0AlaXaa: 0.0 ± 0.0
Cys
0.339CysAla: 0.339 ± 0.171
0.085CysCys: 0.085 ± 0.082
0.254CysAsp: 0.254 ± 0.133
0.424CysGlu: 0.424 ± 0.144
0.593CysPhe: 0.593 ± 0.237
0.424CysGly: 0.424 ± 0.173
0.085CysHis: 0.085 ± 0.082
0.17CysIle: 0.17 ± 0.135
0.254CysLys: 0.254 ± 0.188
0.17CysLeu: 0.17 ± 0.105
0.085CysMet: 0.085 ± 0.082
0.17CysAsn: 0.17 ± 0.113
0.17CysPro: 0.17 ± 0.186
0.17CysGln: 0.17 ± 0.118
0.085CysArg: 0.085 ± 0.091
0.424CysSer: 0.424 ± 0.167
0.254CysThr: 0.254 ± 0.138
0.593CysVal: 0.593 ± 0.263
0.085CysTrp: 0.085 ± 0.08
0.254CysTyr: 0.254 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
5.341AspAla: 5.341 ± 0.744
0.085AspCys: 0.085 ± 0.093
4.663AspAsp: 4.663 ± 1.023
4.917AspGlu: 4.917 ± 0.839
3.306AspPhe: 3.306 ± 0.472
6.359AspGly: 6.359 ± 1.207
0.593AspHis: 0.593 ± 0.229
5.511AspIle: 5.511 ± 0.813
5.256AspLys: 5.256 ± 0.748
4.154AspLeu: 4.154 ± 0.57
2.12AspMet: 2.12 ± 0.401
3.985AspAsn: 3.985 ± 0.591
1.017AspPro: 1.017 ± 0.34
1.102AspGln: 1.102 ± 0.361
2.204AspArg: 2.204 ± 0.509
4.833AspSer: 4.833 ± 0.772
3.815AspThr: 3.815 ± 0.505
3.815AspVal: 3.815 ± 0.57
0.339AspTrp: 0.339 ± 0.207
2.883AspTyr: 2.883 ± 0.623
0.0AspXaa: 0.0 ± 0.0
Glu
3.137GluAla: 3.137 ± 0.532
0.254GluCys: 0.254 ± 0.211
2.967GluAsp: 2.967 ± 0.535
4.409GluGlu: 4.409 ± 0.691
2.459GluPhe: 2.459 ± 0.51
1.865GluGly: 1.865 ± 0.355
1.187GluHis: 1.187 ± 0.334
5.85GluIle: 5.85 ± 0.793
5.002GluLys: 5.002 ± 0.803
7.206GluLeu: 7.206 ± 1.332
1.611GluMet: 1.611 ± 0.528
4.663GluAsn: 4.663 ± 0.563
1.611GluPro: 1.611 ± 0.452
4.324GluGln: 4.324 ± 0.562
4.324GluArg: 4.324 ± 0.722
2.035GluSer: 2.035 ± 0.311
3.815GluThr: 3.815 ± 0.76
4.239GluVal: 4.239 ± 0.704
0.848GluTrp: 0.848 ± 0.279
2.713GluTyr: 2.713 ± 0.552
0.0GluXaa: 0.0 ± 0.0
Phe
2.628PheAla: 2.628 ± 0.318
0.254PheCys: 0.254 ± 0.166
3.9PheAsp: 3.9 ± 0.719
2.543PheGlu: 2.543 ± 0.566
0.848PhePhe: 0.848 ± 0.253
2.035PheGly: 2.035 ± 0.393
0.593PheHis: 0.593 ± 0.181
2.374PheIle: 2.374 ± 0.377
2.883PheLys: 2.883 ± 0.511
2.628PheLeu: 2.628 ± 0.51
0.509PheMet: 0.509 ± 0.216
2.967PheAsn: 2.967 ± 0.607
0.509PhePro: 0.509 ± 0.287
1.187PheGln: 1.187 ± 0.4
1.102PheArg: 1.102 ± 0.252
3.137PheSer: 3.137 ± 0.564
3.052PheThr: 3.052 ± 0.569
2.883PheVal: 2.883 ± 0.554
0.509PheTrp: 0.509 ± 0.255
1.187PheTyr: 1.187 ± 0.314
0.0PheXaa: 0.0 ± 0.0
Gly
5.087GlyAla: 5.087 ± 1.127
0.593GlyCys: 0.593 ± 0.231
3.815GlyAsp: 3.815 ± 0.668
3.561GlyGlu: 3.561 ± 0.474
2.459GlyPhe: 2.459 ± 0.548
4.663GlyGly: 4.663 ± 0.664
1.357GlyHis: 1.357 ± 0.337
6.274GlyIle: 6.274 ± 1.05
6.189GlyLys: 6.189 ± 0.644
4.748GlyLeu: 4.748 ± 0.71
1.95GlyMet: 1.95 ± 0.484
4.833GlyAsn: 4.833 ± 0.942
0.339GlyPro: 0.339 ± 0.135
2.628GlyGln: 2.628 ± 0.548
2.967GlyArg: 2.967 ± 0.663
3.985GlySer: 3.985 ± 1.185
4.748GlyThr: 4.748 ± 0.917
5.341GlyVal: 5.341 ± 0.763
0.763GlyTrp: 0.763 ± 0.284
2.713GlyTyr: 2.713 ± 0.481
0.0GlyXaa: 0.0 ± 0.0
His
0.848HisAla: 0.848 ± 0.239
0.085HisCys: 0.085 ± 0.069
0.678HisAsp: 0.678 ± 0.222
0.933HisGlu: 0.933 ± 0.275
0.763HisPhe: 0.763 ± 0.231
0.933HisGly: 0.933 ± 0.275
0.424HisHis: 0.424 ± 0.193
1.441HisIle: 1.441 ± 0.425
0.763HisLys: 0.763 ± 0.308
1.017HisLeu: 1.017 ± 0.293
0.085HisMet: 0.085 ± 0.091
0.593HisAsn: 0.593 ± 0.196
0.678HisPro: 0.678 ± 0.265
0.848HisGln: 0.848 ± 0.287
0.678HisArg: 0.678 ± 0.227
0.763HisSer: 0.763 ± 0.249
1.102HisThr: 1.102 ± 0.395
0.678HisVal: 0.678 ± 0.262
0.339HisTrp: 0.339 ± 0.186
0.763HisTyr: 0.763 ± 0.254
0.0HisXaa: 0.0 ± 0.0
Ile
5.511IleAla: 5.511 ± 0.89
0.0IleCys: 0.0 ± 0.0
4.917IleAsp: 4.917 ± 0.726
6.189IleGlu: 6.189 ± 0.847
2.374IlePhe: 2.374 ± 0.517
5.511IleGly: 5.511 ± 1.024
1.017IleHis: 1.017 ± 0.283
3.815IleIle: 3.815 ± 0.593
7.461IleLys: 7.461 ± 0.894
3.73IleLeu: 3.73 ± 0.636
1.526IleMet: 1.526 ± 0.397
3.476IleAsn: 3.476 ± 0.678
2.628IlePro: 2.628 ± 0.583
2.459IleGln: 2.459 ± 0.448
3.137IleArg: 3.137 ± 0.498
4.663IleSer: 4.663 ± 0.861
4.833IleThr: 4.833 ± 0.948
4.578IleVal: 4.578 ± 0.692
0.339IleTrp: 0.339 ± 0.175
2.967IleTyr: 2.967 ± 0.683
0.0IleXaa: 0.0 ± 0.0
Lys
6.189LysAla: 6.189 ± 1.008
0.339LysCys: 0.339 ± 0.178
5.85LysAsp: 5.85 ± 0.917
5.511LysGlu: 5.511 ± 0.806
2.713LysPhe: 2.713 ± 0.429
3.815LysGly: 3.815 ± 0.82
0.933LysHis: 0.933 ± 0.288
4.239LysIle: 4.239 ± 0.662
5.85LysLys: 5.85 ± 1.03
6.613LysLeu: 6.613 ± 0.895
2.12LysMet: 2.12 ± 0.524
4.663LysAsn: 4.663 ± 0.518
2.713LysPro: 2.713 ± 0.502
2.459LysGln: 2.459 ± 0.439
4.324LysArg: 4.324 ± 0.796
5.256LysSer: 5.256 ± 0.611
4.663LysThr: 4.663 ± 0.656
6.019LysVal: 6.019 ± 0.969
0.678LysTrp: 0.678 ± 0.265
2.713LysTyr: 2.713 ± 0.465
0.0LysXaa: 0.0 ± 0.0
Leu
6.698LeuAla: 6.698 ± 1.004
0.848LeuCys: 0.848 ± 0.309
5.765LeuAsp: 5.765 ± 0.853
4.578LeuGlu: 4.578 ± 0.77
2.543LeuPhe: 2.543 ± 0.532
4.409LeuGly: 4.409 ± 0.873
0.848LeuHis: 0.848 ± 0.296
4.154LeuIle: 4.154 ± 0.58
6.867LeuLys: 6.867 ± 1.037
4.239LeuLeu: 4.239 ± 0.675
0.933LeuMet: 0.933 ± 0.256
5.765LeuAsn: 5.765 ± 0.838
2.289LeuPro: 2.289 ± 0.479
3.306LeuGln: 3.306 ± 0.6
2.204LeuArg: 2.204 ± 0.417
7.206LeuSer: 7.206 ± 0.775
4.833LeuThr: 4.833 ± 0.554
4.578LeuVal: 4.578 ± 0.655
0.424LeuTrp: 0.424 ± 0.24
2.543LeuTyr: 2.543 ± 0.474
0.0LeuXaa: 0.0 ± 0.0
Met
1.441MetAla: 1.441 ± 0.494
0.085MetCys: 0.085 ± 0.086
1.526MetAsp: 1.526 ± 0.378
0.678MetGlu: 0.678 ± 0.212
0.593MetPhe: 0.593 ± 0.184
1.526MetGly: 1.526 ± 0.326
0.254MetHis: 0.254 ± 0.14
1.78MetIle: 1.78 ± 0.468
2.035MetLys: 2.035 ± 0.436
2.035MetLeu: 2.035 ± 0.322
0.848MetMet: 0.848 ± 0.274
1.187MetAsn: 1.187 ± 0.291
0.593MetPro: 0.593 ± 0.277
1.696MetGln: 1.696 ± 0.415
1.102MetArg: 1.102 ± 0.315
1.696MetSer: 1.696 ± 0.366
2.289MetThr: 2.289 ± 0.353
1.78MetVal: 1.78 ± 0.371
0.339MetTrp: 0.339 ± 0.167
0.933MetTyr: 0.933 ± 0.369
0.0MetXaa: 0.0 ± 0.0
Asn
5.596AsnAla: 5.596 ± 1.015
0.254AsnCys: 0.254 ± 0.163
4.324AsnAsp: 4.324 ± 0.676
3.137AsnGlu: 3.137 ± 0.422
1.696AsnPhe: 1.696 ± 0.303
4.917AsnGly: 4.917 ± 0.874
1.102AsnHis: 1.102 ± 0.291
3.9AsnIle: 3.9 ± 0.922
3.985AsnLys: 3.985 ± 0.524
4.833AsnLeu: 4.833 ± 0.681
1.357AsnMet: 1.357 ± 0.345
3.561AsnAsn: 3.561 ± 0.601
2.967AsnPro: 2.967 ± 0.584
2.289AsnGln: 2.289 ± 0.634
1.696AsnArg: 1.696 ± 0.379
3.306AsnSer: 3.306 ± 0.537
2.713AsnThr: 2.713 ± 0.583
3.815AsnVal: 3.815 ± 0.428
0.593AsnTrp: 0.593 ± 0.259
1.865AsnTyr: 1.865 ± 0.435
0.0AsnXaa: 0.0 ± 0.0
Pro
2.374ProAla: 2.374 ± 0.414
0.254ProCys: 0.254 ± 0.141
1.865ProAsp: 1.865 ± 0.544
1.017ProGlu: 1.017 ± 0.299
1.611ProPhe: 1.611 ± 0.421
1.441ProGly: 1.441 ± 0.438
0.593ProHis: 0.593 ± 0.213
1.611ProIle: 1.611 ± 0.328
2.035ProLys: 2.035 ± 0.45
2.12ProLeu: 2.12 ± 0.439
0.933ProMet: 0.933 ± 0.283
1.78ProAsn: 1.78 ± 0.569
0.593ProPro: 0.593 ± 0.212
1.611ProGln: 1.611 ± 0.55
0.509ProArg: 0.509 ± 0.205
2.035ProSer: 2.035 ± 0.455
2.374ProThr: 2.374 ± 0.506
1.95ProVal: 1.95 ± 0.464
0.339ProTrp: 0.339 ± 0.174
1.441ProTyr: 1.441 ± 0.372
0.0ProXaa: 0.0 ± 0.0
Gln
4.493GlnAla: 4.493 ± 0.738
0.254GlnCys: 0.254 ± 0.147
1.78GlnAsp: 1.78 ± 0.443
2.543GlnGlu: 2.543 ± 0.678
0.848GlnPhe: 0.848 ± 0.263
4.324GlnGly: 4.324 ± 0.985
0.763GlnHis: 0.763 ± 0.302
3.391GlnIle: 3.391 ± 0.642
2.543GlnLys: 2.543 ± 0.503
3.052GlnLeu: 3.052 ± 0.581
1.357GlnMet: 1.357 ± 0.294
1.78GlnAsn: 1.78 ± 0.478
1.017GlnPro: 1.017 ± 0.344
2.543GlnGln: 2.543 ± 0.768
1.611GlnArg: 1.611 ± 0.328
2.883GlnSer: 2.883 ± 0.597
1.95GlnThr: 1.95 ± 0.426
2.883GlnVal: 2.883 ± 0.413
0.678GlnTrp: 0.678 ± 0.255
1.611GlnTyr: 1.611 ± 0.352
0.0GlnXaa: 0.0 ± 0.0
Arg
2.628ArgAla: 2.628 ± 0.55
0.085ArgCys: 0.085 ± 0.093
1.95ArgAsp: 1.95 ± 0.337
1.865ArgGlu: 1.865 ± 0.326
1.017ArgPhe: 1.017 ± 0.346
2.628ArgGly: 2.628 ± 0.458
0.933ArgHis: 0.933 ± 0.231
2.459ArgIle: 2.459 ± 0.432
3.73ArgLys: 3.73 ± 0.62
2.628ArgLeu: 2.628 ± 0.506
1.102ArgMet: 1.102 ± 0.309
2.12ArgAsn: 2.12 ± 0.365
1.526ArgPro: 1.526 ± 0.348
1.441ArgGln: 1.441 ± 0.373
1.441ArgArg: 1.441 ± 0.414
2.374ArgSer: 2.374 ± 0.475
2.798ArgThr: 2.798 ± 0.647
2.289ArgVal: 2.289 ± 0.413
1.357ArgTrp: 1.357 ± 0.399
2.035ArgTyr: 2.035 ± 0.378
0.0ArgXaa: 0.0 ± 0.0
Ser
5.511SerAla: 5.511 ± 0.905
0.17SerCys: 0.17 ± 0.1
4.748SerAsp: 4.748 ± 0.888
3.9SerGlu: 3.9 ± 0.689
3.222SerPhe: 3.222 ± 0.611
5.765SerGly: 5.765 ± 1.589
0.509SerHis: 0.509 ± 0.166
2.967SerIle: 2.967 ± 0.593
3.561SerLys: 3.561 ± 0.516
5.85SerLeu: 5.85 ± 0.781
1.78SerMet: 1.78 ± 0.506
3.646SerAsn: 3.646 ± 0.641
1.865SerPro: 1.865 ± 0.403
3.052SerGln: 3.052 ± 0.426
1.865SerArg: 1.865 ± 0.404
5.256SerSer: 5.256 ± 0.835
4.663SerThr: 4.663 ± 0.892
3.815SerVal: 3.815 ± 0.803
1.357SerTrp: 1.357 ± 0.423
2.374SerTyr: 2.374 ± 0.423
0.0SerXaa: 0.0 ± 0.0
Thr
4.324ThrAla: 4.324 ± 0.752
0.424ThrCys: 0.424 ± 0.208
3.985ThrAsp: 3.985 ± 0.848
3.476ThrGlu: 3.476 ± 0.805
3.222ThrPhe: 3.222 ± 0.428
5.341ThrGly: 5.341 ± 0.633
1.102ThrHis: 1.102 ± 0.321
5.765ThrIle: 5.765 ± 0.812
5.172ThrLys: 5.172 ± 0.556
5.087ThrLeu: 5.087 ± 0.943
1.526ThrMet: 1.526 ± 0.305
2.289ThrAsn: 2.289 ± 0.509
2.374ThrPro: 2.374 ± 0.592
2.713ThrGln: 2.713 ± 0.576
2.204ThrArg: 2.204 ± 0.412
2.713ThrSer: 2.713 ± 0.567
4.493ThrThr: 4.493 ± 0.654
6.189ThrVal: 6.189 ± 0.709
0.763ThrTrp: 0.763 ± 0.28
2.713ThrTyr: 2.713 ± 0.51
0.0ThrXaa: 0.0 ± 0.0
Val
5.426ValAla: 5.426 ± 0.818
0.17ValCys: 0.17 ± 0.129
4.578ValAsp: 4.578 ± 0.682
5.68ValGlu: 5.68 ± 0.71
2.798ValPhe: 2.798 ± 0.405
4.663ValGly: 4.663 ± 0.567
0.678ValHis: 0.678 ± 0.244
4.578ValIle: 4.578 ± 0.694
4.154ValLys: 4.154 ± 0.702
4.07ValLeu: 4.07 ± 0.649
1.272ValMet: 1.272 ± 0.315
2.798ValAsn: 2.798 ± 0.417
1.865ValPro: 1.865 ± 0.391
2.459ValGln: 2.459 ± 0.565
1.696ValArg: 1.696 ± 0.336
6.189ValSer: 6.189 ± 0.824
5.256ValThr: 5.256 ± 0.71
4.409ValVal: 4.409 ± 0.742
1.187ValTrp: 1.187 ± 0.469
3.646ValTyr: 3.646 ± 0.621
0.0ValXaa: 0.0 ± 0.0
Trp
1.017TrpAla: 1.017 ± 0.312
0.0TrpCys: 0.0 ± 0.0
0.593TrpAsp: 0.593 ± 0.192
0.763TrpGlu: 0.763 ± 0.361
0.254TrpPhe: 0.254 ± 0.152
1.102TrpGly: 1.102 ± 0.453
0.17TrpHis: 0.17 ± 0.122
1.272TrpIle: 1.272 ± 0.461
0.933TrpLys: 0.933 ± 0.326
0.763TrpLeu: 0.763 ± 0.267
0.17TrpMet: 0.17 ± 0.119
0.339TrpAsn: 0.339 ± 0.166
0.0TrpPro: 0.0 ± 0.0
0.678TrpGln: 0.678 ± 0.294
1.017TrpArg: 1.017 ± 0.252
1.017TrpSer: 1.017 ± 0.277
1.017TrpThr: 1.017 ± 0.428
0.678TrpVal: 0.678 ± 0.251
0.17TrpTrp: 0.17 ± 0.176
0.763TrpTyr: 0.763 ± 0.364
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.374TyrAla: 2.374 ± 0.494
0.593TyrCys: 0.593 ± 0.258
3.137TyrAsp: 3.137 ± 0.634
2.035TyrGlu: 2.035 ± 0.396
1.78TyrPhe: 1.78 ± 0.523
2.035TyrGly: 2.035 ± 0.406
0.593TyrHis: 0.593 ± 0.278
3.306TyrIle: 3.306 ± 0.714
2.798TyrLys: 2.798 ± 0.364
3.476TyrLeu: 3.476 ± 0.649
0.933TyrMet: 0.933 ± 0.28
2.204TyrAsn: 2.204 ± 0.582
1.272TyrPro: 1.272 ± 0.331
2.459TyrGln: 2.459 ± 0.384
2.289TyrArg: 2.289 ± 0.441
1.95TyrSer: 1.95 ± 0.395
2.628TyrThr: 2.628 ± 0.525
2.543TyrVal: 2.543 ± 0.417
0.509TyrTrp: 0.509 ± 0.213
1.78TyrTyr: 1.78 ± 0.45
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (11796 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski