Qingshengfaniella alkalisoli

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Qingshengfaniella

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3573 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8IUC8|A0A5B8IUC8_9RHOB Uncharacterized protein OS=Qingshengfaniella alkalisoli OX=2599296 GN=FPZ52_06130 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84LYY4 pKa = 10.84ASCAALALGLAAGGAHH20 pKa = 5.93AQSGEE25 pKa = 3.85ITVWSWNIAASSLEE39 pKa = 4.03SVIEE43 pKa = 4.22GFNAEE48 pKa = 4.26YY49 pKa = 10.26PDD51 pKa = 3.55VTVTVEE57 pKa = 4.39DD58 pKa = 4.5LGNQQVFDD66 pKa = 3.86RR67 pKa = 11.84TLAGCAAGGSGLPDD81 pKa = 3.07VLSIEE86 pKa = 4.02NHH88 pKa = 4.75EE89 pKa = 5.05AEE91 pKa = 4.61IFWAQFPDD99 pKa = 3.86CFANLRR105 pKa = 11.84DD106 pKa = 3.67LGYY109 pKa = 10.73DD110 pKa = 3.49EE111 pKa = 5.43EE112 pKa = 5.26SSARR116 pKa = 11.84FPDD119 pKa = 4.97FKK121 pKa = 10.47RR122 pKa = 11.84TEE124 pKa = 4.17LEE126 pKa = 4.2VGDD129 pKa = 4.1VAYY132 pKa = 10.7AMPWDD137 pKa = 4.19SGPVAMFYY145 pKa = 10.74RR146 pKa = 11.84RR147 pKa = 11.84DD148 pKa = 3.71FYY150 pKa = 11.72DD151 pKa = 3.32NAGVDD156 pKa = 3.64PASIQTWDD164 pKa = 3.76DD165 pKa = 4.49FIAAGKK171 pKa = 10.24QIMEE175 pKa = 4.59ANDD178 pKa = 3.88GVTMTNADD186 pKa = 3.96LNGDD190 pKa = 4.03TEE192 pKa = 4.61WFRR195 pKa = 11.84MIANEE200 pKa = 3.9QGCGYY205 pKa = 9.95FSNDD209 pKa = 3.15GEE211 pKa = 5.03SITVNQPSCVAALDD225 pKa = 4.09VVKK228 pKa = 10.96SLVDD232 pKa = 3.64EE233 pKa = 4.93GLMTAADD240 pKa = 4.04WNEE243 pKa = 3.93KK244 pKa = 8.91IQNNNAGAVASQMYY258 pKa = 8.99GGWYY262 pKa = 9.51EE263 pKa = 3.87GTIRR267 pKa = 11.84STAPEE272 pKa = 4.17DD273 pKa = 3.75QAGQWGVYY281 pKa = 10.01LMPSLSADD289 pKa = 3.46GAHH292 pKa = 6.93AANLGGSSLAIPANSEE308 pKa = 3.8NKK310 pKa = 8.19EE311 pKa = 4.01AAWAYY316 pKa = 10.69VNYY319 pKa = 10.87ALGTNDD325 pKa = 3.97GQVTMLKK332 pKa = 10.29SYY334 pKa = 11.1GLVPSLLSALEE345 pKa = 4.12DD346 pKa = 4.4PYY348 pKa = 11.78VQDD351 pKa = 5.64GLPFWGDD358 pKa = 3.23QPVWEE363 pKa = 5.17TILGTLDD370 pKa = 3.31QINPMRR376 pKa = 11.84GTSFFGDD383 pKa = 3.33ADD385 pKa = 3.84QIVQTVQTQYY395 pKa = 11.87LNGGYY400 pKa = 10.46DD401 pKa = 3.37SAQAALDD408 pKa = 3.91DD409 pKa = 3.97AAKK412 pKa = 10.37QIEE415 pKa = 5.23LVTGIPAAQQ424 pKa = 3.19

Molecular weight: 45.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8J6R1|A0A5B8J6R1_9RHOB DUF882 domain-containing protein OS=Qingshengfaniella alkalisoli OX=2599296 GN=FPZ52_11175 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3573

0

3573

1157484

25

5139

324.0

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.322 ± 0.051

0.883 ± 0.014

6.432 ± 0.057

5.911 ± 0.036

3.761 ± 0.026

8.452 ± 0.048

2.016 ± 0.021

5.482 ± 0.029

3.181 ± 0.038

10.013 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.698 ± 0.021

2.8 ± 0.024

4.967 ± 0.025

3.291 ± 0.022

6.574 ± 0.056

5.612 ± 0.03

5.593 ± 0.056

7.38 ± 0.039

1.357 ± 0.019

2.276 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski