Nitrospirae bacterium HCH-1

Taxonomy: cellular organisms; Bacteria; Nitrospirae; unclassified Nitrospirae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A109BTY7|A0A109BTY7_9BACT Potassium transporter TrkA OS=Nitrospirae bacterium HCH-1 OX=1748249 GN=ASN18_3310 PE=4 SV=1
MM1 pKa = 7.03VHH3 pKa = 6.71VDD5 pKa = 5.04DD6 pKa = 5.83PPLDD10 pKa = 3.74TLVGVQLIVPPAPADD25 pKa = 3.54ADD27 pKa = 3.59TAYY30 pKa = 9.75WFNVKK35 pKa = 10.1LAVTEE40 pKa = 3.98QLLVTLVTVYY50 pKa = 9.71VFPDD54 pKa = 3.87NVPPPQVSLTDD65 pKa = 3.08AVYY68 pKa = 9.54PVFGVTVNVVEE79 pKa = 4.71PPLATVFEE87 pKa = 5.01AGDD90 pKa = 3.84MVPPEE95 pKa = 4.6PLTEE99 pKa = 3.96AVTVSAGVNVAAKK112 pKa = 9.83LHH114 pKa = 6.26APVIGAVVNVLPFSVPEE131 pKa = 3.8QPVTVFIWYY140 pKa = 7.69PAFGVSVKK148 pKa = 10.58LAVLPCFTVWLAGLTLPPPPLTDD171 pKa = 3.82AVILL175 pKa = 4.07

Molecular weight: 18.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A109C2R1|A0A109C2R1_9BACT Desulforedoxin OS=Nitrospirae bacterium HCH-1 OX=1748249 GN=ASN18_2726 PE=4 SV=1
MM1 pKa = 7.33AAKK4 pKa = 9.87EE5 pKa = 4.31SKK7 pKa = 10.12EE8 pKa = 3.49EE9 pKa = 3.77HH10 pKa = 5.67RR11 pKa = 11.84RR12 pKa = 11.84LRR14 pKa = 11.84RR15 pKa = 11.84AEE17 pKa = 3.88RR18 pKa = 11.84LLEE21 pKa = 4.16NLSMPSRR28 pKa = 11.84PDD30 pKa = 3.19VLLDD34 pKa = 3.82AVRR37 pKa = 11.84TQAGFAPDD45 pKa = 4.26PLAVTAVILKK55 pKa = 10.72DD56 pKa = 3.51MALSAAVLHH65 pKa = 6.61AANTQLNGWKK75 pKa = 9.74RR76 pKa = 11.84KK77 pKa = 9.53LISIEE82 pKa = 3.87GAVALLGLEE91 pKa = 3.9RR92 pKa = 11.84VRR94 pKa = 11.84AIVAEE99 pKa = 4.16QFLSATLVSQEE110 pKa = 4.73GPLQMVRR117 pKa = 11.84LRR119 pKa = 11.84GVEE122 pKa = 3.87AGRR125 pKa = 11.84VAARR129 pKa = 11.84LARR132 pKa = 11.84EE133 pKa = 4.11LPKK136 pKa = 10.52AAPHH140 pKa = 6.33CLNGYY145 pKa = 8.59LPPVAPDD152 pKa = 3.06EE153 pKa = 4.79AYY155 pKa = 10.37AAGLLHH161 pKa = 7.47DD162 pKa = 5.15CGLVAMMRR170 pKa = 11.84GFPDD174 pKa = 3.35YY175 pKa = 11.41VGFCQEE181 pKa = 3.38MHH183 pKa = 6.21ARR185 pKa = 11.84GGGRR189 pKa = 11.84RR190 pKa = 11.84GGGGEE195 pKa = 3.8RR196 pKa = 11.84AFRR199 pKa = 11.84DD200 pKa = 3.61QPLSGGFFDD209 pKa = 4.29GAEE212 pKa = 4.09MASAGTFLSDD222 pKa = 3.56HH223 pKa = 6.04SHH225 pKa = 6.89PSSDD229 pKa = 3.92GRR231 pKa = 11.84LQPSRR236 pKa = 11.84TEE238 pKa = 3.99GAGTRR243 pKa = 11.84LYY245 pKa = 11.23GSAGHH250 pKa = 5.95TAPFRR255 pKa = 11.84LGLRR259 pKa = 11.84WGVGGGVAGRR269 pKa = 11.84GRR271 pKa = 11.84AHH273 pKa = 7.22HH274 pKa = 6.34LFFWFGSRR282 pKa = 11.84ATGTIARR289 pKa = 11.84PLRR292 pKa = 11.84WGVRR296 pKa = 11.84RR297 pKa = 11.84RR298 pKa = 11.84GWLFSEE304 pKa = 4.99KK305 pKa = 10.27YY306 pKa = 9.22VSIWEE311 pKa = 4.04MKK313 pKa = 9.12RR314 pKa = 11.84AGRR317 pKa = 11.84IHH319 pKa = 7.37RR320 pKa = 11.84GTFLGGG326 pKa = 3.07

Molecular weight: 35.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3334

0

3334

1080144

29

2156

324.0

36.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.903 ± 0.051

1.149 ± 0.018

5.558 ± 0.03

6.239 ± 0.049

4.258 ± 0.031

6.917 ± 0.039

1.889 ± 0.02

8.094 ± 0.042

6.83 ± 0.038

9.335 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.751 ± 0.018

4.225 ± 0.028

3.811 ± 0.028

2.756 ± 0.021

4.659 ± 0.034

6.414 ± 0.041

5.794 ± 0.037

6.829 ± 0.031

0.941 ± 0.016

3.648 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski