Amino acid dipepetide frequency for Nitrospirae bacterium HCH-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.403AlaAla: 9.403 ± 0.133
0.824AlaCys: 0.824 ± 0.039
4.089AlaAsp: 4.089 ± 0.061
4.92AlaGlu: 4.92 ± 0.071
3.138AlaPhe: 3.138 ± 0.056
5.939AlaGly: 5.939 ± 0.089
1.419AlaHis: 1.419 ± 0.038
6.104AlaIle: 6.104 ± 0.078
4.375AlaLys: 4.375 ± 0.071
8.276AlaLeu: 8.276 ± 0.102
2.375AlaMet: 2.375 ± 0.053
2.503AlaAsn: 2.503 ± 0.048
2.268AlaPro: 2.268 ± 0.044
2.116AlaGln: 2.116 ± 0.047
3.306AlaArg: 3.306 ± 0.062
4.761AlaSer: 4.761 ± 0.073
3.222AlaThr: 3.222 ± 0.063
6.881AlaVal: 6.881 ± 0.099
0.653AlaTrp: 0.653 ± 0.031
2.461AlaTyr: 2.461 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.827CysAla: 0.827 ± 0.033
0.162CysCys: 0.162 ± 0.012
0.562CysAsp: 0.562 ± 0.021
0.63CysGlu: 0.63 ± 0.024
0.456CysPhe: 0.456 ± 0.024
1.079CysGly: 1.079 ± 0.04
0.351CysHis: 0.351 ± 0.021
0.763CysIle: 0.763 ± 0.029
0.668CysLys: 0.668 ± 0.026
1.068CysLeu: 1.068 ± 0.035
0.254CysMet: 0.254 ± 0.014
0.48CysAsn: 0.48 ± 0.02
0.616CysPro: 0.616 ± 0.03
0.286CysGln: 0.286 ± 0.016
0.577CysArg: 0.577 ± 0.024
0.776CysSer: 0.776 ± 0.031
0.618CysThr: 0.618 ± 0.027
0.773CysVal: 0.773 ± 0.028
0.121CysTrp: 0.121 ± 0.012
0.424CysTyr: 0.424 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
4.13AspAla: 4.13 ± 0.064
0.692AspCys: 0.692 ± 0.026
3.067AspAsp: 3.067 ± 0.05
3.874AspGlu: 3.874 ± 0.068
2.627AspPhe: 2.627 ± 0.053
3.961AspGly: 3.961 ± 0.068
0.707AspHis: 0.707 ± 0.025
5.624AspIle: 5.624 ± 0.088
4.162AspLys: 4.162 ± 0.066
4.164AspLeu: 4.164 ± 0.063
1.812AspMet: 1.812 ± 0.045
2.583AspAsn: 2.583 ± 0.055
1.834AspPro: 1.834 ± 0.038
0.83AspGln: 0.83 ± 0.029
2.211AspArg: 2.211 ± 0.052
3.238AspSer: 3.238 ± 0.06
3.419AspThr: 3.419 ± 0.059
3.747AspVal: 3.747 ± 0.065
0.561AspTrp: 0.561 ± 0.025
2.334AspTyr: 2.334 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
5.227GluAla: 5.227 ± 0.072
0.533GluCys: 0.533 ± 0.025
3.258GluAsp: 3.258 ± 0.051
4.105GluGlu: 4.105 ± 0.073
2.586GluPhe: 2.586 ± 0.056
4.108GluGly: 4.108 ± 0.067
1.17GluHis: 1.17 ± 0.032
5.706GluIle: 5.706 ± 0.08
4.832GluLys: 4.832 ± 0.083
6.564GluLeu: 6.564 ± 0.1
1.728GluMet: 1.728 ± 0.046
2.473GluAsn: 2.473 ± 0.046
1.903GluPro: 1.903 ± 0.048
2.04GluGln: 2.04 ± 0.051
3.386GluArg: 3.386 ± 0.067
3.127GluSer: 3.127 ± 0.063
3.567GluThr: 3.567 ± 0.055
3.768GluVal: 3.768 ± 0.059
0.414GluTrp: 0.414 ± 0.021
1.896GluTyr: 1.896 ± 0.044
0.0GluXaa: 0.0 ± 0.0
Phe
2.814PheAla: 2.814 ± 0.058
0.527PheCys: 0.527 ± 0.025
2.545PheAsp: 2.545 ± 0.047
2.144PheGlu: 2.144 ± 0.048
2.099PhePhe: 2.099 ± 0.047
2.783PheGly: 2.783 ± 0.052
0.801PheHis: 0.801 ± 0.03
3.666PheIle: 3.666 ± 0.064
2.799PheLys: 2.799 ± 0.056
3.879PheLeu: 3.879 ± 0.077
1.141PheMet: 1.141 ± 0.033
2.192PheAsn: 2.192 ± 0.046
1.543PhePro: 1.543 ± 0.038
1.053PheGln: 1.053 ± 0.031
1.756PheArg: 1.756 ± 0.041
3.097PheSer: 3.097 ± 0.055
2.831PheThr: 2.831 ± 0.054
2.701PheVal: 2.701 ± 0.058
0.448PheTrp: 0.448 ± 0.02
1.759PheTyr: 1.759 ± 0.047
0.0PheXaa: 0.0 ± 0.0
Gly
5.174GlyAla: 5.174 ± 0.085
0.944GlyCys: 0.944 ± 0.035
3.458GlyAsp: 3.458 ± 0.061
3.666GlyGlu: 3.666 ± 0.059
3.207GlyPhe: 3.207 ± 0.057
5.071GlyGly: 5.071 ± 0.101
1.451GlyHis: 1.451 ± 0.042
5.99GlyIle: 5.99 ± 0.074
4.843GlyLys: 4.843 ± 0.072
6.62GlyLeu: 6.62 ± 0.078
2.034GlyMet: 2.034 ± 0.046
2.469GlyAsn: 2.469 ± 0.066
1.503GlyPro: 1.503 ± 0.043
2.234GlyGln: 2.234 ± 0.053
3.498GlyArg: 3.498 ± 0.058
4.245GlySer: 4.245 ± 0.074
4.249GlyThr: 4.249 ± 0.1
5.106GlyVal: 5.106 ± 0.075
0.687GlyTrp: 0.687 ± 0.03
2.72GlyTyr: 2.72 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.252HisAla: 1.252 ± 0.037
0.248HisCys: 0.248 ± 0.016
1.02HisAsp: 1.02 ± 0.032
1.174HisGlu: 1.174 ± 0.034
0.797HisPhe: 0.797 ± 0.027
1.408HisGly: 1.408 ± 0.041
0.494HisHis: 0.494 ± 0.025
1.59HisIle: 1.59 ± 0.041
1.151HisLys: 1.151 ± 0.031
1.534HisLeu: 1.534 ± 0.038
0.547HisMet: 0.547 ± 0.019
0.818HisAsn: 0.818 ± 0.029
0.991HisPro: 0.991 ± 0.031
0.507HisGln: 0.507 ± 0.022
0.93HisArg: 0.93 ± 0.031
1.2HisSer: 1.2 ± 0.032
1.137HisThr: 1.137 ± 0.037
1.167HisVal: 1.167 ± 0.034
0.212HisTrp: 0.212 ± 0.014
0.711HisTyr: 0.711 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
6.461IleAla: 6.461 ± 0.09
0.879IleCys: 0.879 ± 0.033
4.999IleAsp: 4.999 ± 0.078
5.778IleGlu: 5.778 ± 0.094
3.193IlePhe: 3.193 ± 0.059
5.27IleGly: 5.27 ± 0.081
1.406IleHis: 1.406 ± 0.037
6.117IleIle: 6.117 ± 0.092
5.87IleLys: 5.87 ± 0.08
6.769IleLeu: 6.769 ± 0.083
1.936IleMet: 1.936 ± 0.045
3.972IleAsn: 3.972 ± 0.061
3.271IlePro: 3.271 ± 0.062
1.82IleGln: 1.82 ± 0.043
3.609IleArg: 3.609 ± 0.059
5.491IleSer: 5.491 ± 0.073
5.214IleThr: 5.214 ± 0.079
5.672IleVal: 5.672 ± 0.074
0.565IleTrp: 0.565 ± 0.021
2.651IleTyr: 2.651 ± 0.059
0.0IleXaa: 0.0 ± 0.0
Lys
5.146LysAla: 5.146 ± 0.072
0.654LysCys: 0.654 ± 0.028
4.572LysAsp: 4.572 ± 0.083
5.173LysGlu: 5.173 ± 0.079
1.943LysPhe: 1.943 ± 0.049
4.7LysGly: 4.7 ± 0.075
1.329LysHis: 1.329 ± 0.034
5.347LysIle: 5.347 ± 0.08
5.358LysLys: 5.358 ± 0.084
5.484LysLeu: 5.484 ± 0.078
1.867LysMet: 1.867 ± 0.049
3.16LysAsn: 3.16 ± 0.059
2.606LysPro: 2.606 ± 0.05
1.959LysGln: 1.959 ± 0.039
3.297LysArg: 3.297 ± 0.059
4.026LysSer: 4.026 ± 0.061
4.457LysThr: 4.457 ± 0.073
3.901LysVal: 3.901 ± 0.065
0.637LysTrp: 0.637 ± 0.026
2.688LysTyr: 2.688 ± 0.054
0.0LysXaa: 0.0 ± 0.0
Leu
6.28LeuAla: 6.28 ± 0.084
1.179LeuCys: 1.179 ± 0.031
4.792LeuAsp: 4.792 ± 0.067
4.95LeuGlu: 4.95 ± 0.087
4.044LeuPhe: 4.044 ± 0.083
5.578LeuGly: 5.578 ± 0.083
1.671LeuHis: 1.671 ± 0.035
7.234LeuIle: 7.234 ± 0.089
7.417LeuLys: 7.417 ± 0.094
8.622LeuLeu: 8.622 ± 0.102
2.554LeuMet: 2.554 ± 0.053
4.452LeuAsn: 4.452 ± 0.074
3.794LeuPro: 3.794 ± 0.063
2.596LeuGln: 2.596 ± 0.049
5.159LeuArg: 5.159 ± 0.078
7.576LeuSer: 7.576 ± 0.099
5.89LeuThr: 5.89 ± 0.081
5.238LeuVal: 5.238 ± 0.078
0.941LeuTrp: 0.941 ± 0.042
3.385LeuTyr: 3.385 ± 0.063
0.0LeuXaa: 0.0 ± 0.0
Met
2.36MetAla: 2.36 ± 0.056
0.27MetCys: 0.27 ± 0.018
1.522MetAsp: 1.522 ± 0.039
1.767MetGlu: 1.767 ± 0.041
1.125MetPhe: 1.125 ± 0.033
1.913MetGly: 1.913 ± 0.042
0.421MetHis: 0.421 ± 0.019
1.766MetIle: 1.766 ± 0.041
2.071MetLys: 2.071 ± 0.044
2.774MetLeu: 2.774 ± 0.053
0.723MetMet: 0.723 ± 0.026
1.125MetAsn: 1.125 ± 0.037
1.375MetPro: 1.375 ± 0.037
0.7MetGln: 0.7 ± 0.028
1.352MetArg: 1.352 ± 0.035
1.936MetSer: 1.936 ± 0.045
1.649MetThr: 1.649 ± 0.038
1.706MetVal: 1.706 ± 0.04
0.241MetTrp: 0.241 ± 0.013
0.717MetTyr: 0.717 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.206AsnAla: 3.206 ± 0.057
0.493AsnCys: 0.493 ± 0.022
2.187AsnAsp: 2.187 ± 0.044
2.414AsnGlu: 2.414 ± 0.054
1.545AsnPhe: 1.545 ± 0.039
2.778AsnGly: 2.778 ± 0.061
0.686AsnHis: 0.686 ± 0.029
4.038AsnIle: 4.038 ± 0.065
2.917AsnLys: 2.917 ± 0.055
3.68AsnLeu: 3.68 ± 0.065
1.199AsnMet: 1.199 ± 0.031
2.081AsnAsn: 2.081 ± 0.048
2.152AsnPro: 2.152 ± 0.05
0.996AsnGln: 0.996 ± 0.031
1.909AsnArg: 1.909 ± 0.043
2.709AsnSer: 2.709 ± 0.062
2.575AsnThr: 2.575 ± 0.056
2.659AsnVal: 2.659 ± 0.047
0.443AsnTrp: 0.443 ± 0.022
1.585AsnTyr: 1.585 ± 0.044
0.0AsnXaa: 0.0 ± 0.0
Pro
2.947ProAla: 2.947 ± 0.064
0.396ProCys: 0.396 ± 0.02
2.673ProAsp: 2.673 ± 0.055
3.051ProGlu: 3.051 ± 0.064
1.83ProPhe: 1.83 ± 0.044
2.426ProGly: 2.426 ± 0.053
0.772ProHis: 0.772 ± 0.029
2.441ProIle: 2.441 ± 0.051
1.984ProLys: 1.984 ± 0.041
3.591ProLeu: 3.591 ± 0.063
0.996ProMet: 0.996 ± 0.027
1.365ProAsn: 1.365 ± 0.034
1.723ProPro: 1.723 ± 0.063
1.194ProGln: 1.194 ± 0.041
1.292ProArg: 1.292 ± 0.04
2.343ProSer: 2.343 ± 0.051
1.761ProThr: 1.761 ± 0.048
3.415ProVal: 3.415 ± 0.055
0.37ProTrp: 0.37 ± 0.018
1.54ProTyr: 1.54 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
2.116GlnAla: 2.116 ± 0.039
0.357GlnCys: 0.357 ± 0.018
1.296GlnAsp: 1.296 ± 0.037
1.503GlnGlu: 1.503 ± 0.035
1.054GlnPhe: 1.054 ± 0.034
1.637GlnGly: 1.637 ± 0.038
0.579GlnHis: 0.579 ± 0.026
2.122GlnIle: 2.122 ± 0.052
1.941GlnLys: 1.941 ± 0.042
2.659GlnLeu: 2.659 ± 0.053
0.898GlnMet: 0.898 ± 0.028
1.107GlnAsn: 1.107 ± 0.036
1.059GlnPro: 1.059 ± 0.03
1.015GlnGln: 1.015 ± 0.039
1.678GlnArg: 1.678 ± 0.042
1.852GlnSer: 1.852 ± 0.042
1.648GlnThr: 1.648 ± 0.043
1.59GlnVal: 1.59 ± 0.037
0.397GlnTrp: 0.397 ± 0.021
1.051GlnTyr: 1.051 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
3.411ArgAla: 3.411 ± 0.054
0.602ArgCys: 0.602 ± 0.029
2.582ArgAsp: 2.582 ± 0.061
3.185ArgGlu: 3.185 ± 0.069
2.173ArgPhe: 2.173 ± 0.048
2.872ArgGly: 2.872 ± 0.048
1.152ArgHis: 1.152 ± 0.034
3.395ArgIle: 3.395 ± 0.056
3.115ArgLys: 3.115 ± 0.053
5.08ArgLeu: 5.08 ± 0.083
1.233ArgMet: 1.233 ± 0.03
1.776ArgAsn: 1.776 ± 0.035
1.828ArgPro: 1.828 ± 0.041
1.896ArgGln: 1.896 ± 0.041
2.502ArgArg: 2.502 ± 0.059
2.191ArgSer: 2.191 ± 0.048
2.277ArgThr: 2.277 ± 0.042
2.894ArgVal: 2.894 ± 0.054
0.452ArgTrp: 0.452 ± 0.02
1.972ArgTyr: 1.972 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
5.212SerAla: 5.212 ± 0.079
0.736SerCys: 0.736 ± 0.029
3.495SerAsp: 3.495 ± 0.062
3.712SerGlu: 3.712 ± 0.066
2.863SerPhe: 2.863 ± 0.057
5.392SerGly: 5.392 ± 0.1
1.253SerHis: 1.253 ± 0.03
5.072SerIle: 5.072 ± 0.069
3.796SerLys: 3.796 ± 0.07
5.94SerLeu: 5.94 ± 0.079
1.775SerMet: 1.775 ± 0.046
2.42SerAsn: 2.42 ± 0.047
2.475SerPro: 2.475 ± 0.058
1.689SerGln: 1.689 ± 0.046
2.698SerArg: 2.698 ± 0.053
4.423SerSer: 4.423 ± 0.095
3.521SerThr: 3.521 ± 0.074
4.686SerVal: 4.686 ± 0.068
0.6SerTrp: 0.6 ± 0.025
2.38SerTyr: 2.38 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
5.503ThrAla: 5.503 ± 0.086
0.551ThrCys: 0.551 ± 0.024
3.051ThrAsp: 3.051 ± 0.056
3.278ThrGlu: 3.278 ± 0.066
2.184ThrPhe: 2.184 ± 0.051
5.159ThrGly: 5.159 ± 0.082
1.163ThrHis: 1.163 ± 0.037
4.598ThrIle: 4.598 ± 0.071
3.148ThrLys: 3.148 ± 0.055
5.688ThrLeu: 5.688 ± 0.079
1.381ThrMet: 1.381 ± 0.039
2.155ThrAsn: 2.155 ± 0.052
2.734ThrPro: 2.734 ± 0.056
1.646ThrGln: 1.646 ± 0.037
2.176ThrArg: 2.176 ± 0.045
3.437ThrSer: 3.437 ± 0.087
3.255ThrThr: 3.255 ± 0.084
4.414ThrVal: 4.414 ± 0.082
0.5ThrTrp: 0.5 ± 0.022
1.924ThrTyr: 1.924 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
4.429ValAla: 4.429 ± 0.07
0.846ValCys: 0.846 ± 0.031
3.602ValAsp: 3.602 ± 0.062
4.286ValGlu: 4.286 ± 0.074
3.447ValPhe: 3.447 ± 0.063
3.953ValGly: 3.953 ± 0.065
1.131ValHis: 1.131 ± 0.038
5.896ValIle: 5.896 ± 0.072
4.701ValLys: 4.701 ± 0.062
6.615ValLeu: 6.615 ± 0.078
1.911ValMet: 1.911 ± 0.037
2.967ValAsn: 2.967 ± 0.05
2.602ValPro: 2.602 ± 0.048
1.697ValGln: 1.697 ± 0.046
3.055ValArg: 3.055 ± 0.056
4.87ValSer: 4.87 ± 0.067
4.13ValThr: 4.13 ± 0.076
5.134ValVal: 5.134 ± 0.083
0.626ValTrp: 0.626 ± 0.026
2.389ValTyr: 2.389 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.628TrpAla: 0.628 ± 0.028
0.126TrpCys: 0.126 ± 0.01
0.565TrpAsp: 0.565 ± 0.028
0.53TrpGlu: 0.53 ± 0.025
0.441TrpPhe: 0.441 ± 0.026
0.683TrpGly: 0.683 ± 0.037
0.244TrpHis: 0.244 ± 0.014
0.75TrpIle: 0.75 ± 0.025
0.622TrpLys: 0.622 ± 0.026
0.942TrpLeu: 0.942 ± 0.034
0.232TrpMet: 0.232 ± 0.015
0.403TrpAsn: 0.403 ± 0.023
0.335TrpPro: 0.335 ± 0.021
0.373TrpGln: 0.373 ± 0.018
0.492TrpArg: 0.492 ± 0.021
0.557TrpSer: 0.557 ± 0.025
0.472TrpThr: 0.472 ± 0.025
0.561TrpVal: 0.561 ± 0.022
0.121TrpTrp: 0.121 ± 0.012
0.329TrpTyr: 0.329 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.508TyrAla: 2.508 ± 0.053
0.473TyrCys: 0.473 ± 0.021
2.243TyrAsp: 2.243 ± 0.047
2.25TyrGlu: 2.25 ± 0.056
1.827TyrPhe: 1.827 ± 0.046
2.46TyrGly: 2.46 ± 0.048
0.686TyrHis: 0.686 ± 0.028
2.724TyrIle: 2.724 ± 0.06
2.534TyrLys: 2.534 ± 0.054
3.4TyrLeu: 3.4 ± 0.062
0.916TyrMet: 0.916 ± 0.03
1.751TyrAsn: 1.751 ± 0.044
1.499TyrPro: 1.499 ± 0.035
0.91TyrGln: 0.91 ± 0.027
1.706TyrArg: 1.706 ± 0.041
2.281TyrSer: 2.281 ± 0.051
2.052TyrThr: 2.052 ± 0.046
2.277TyrVal: 2.277 ± 0.045
0.418TyrTrp: 0.418 ± 0.02
1.569TyrTyr: 1.569 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3334 proteins (1080144 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski